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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSI221
Library VS
(Link to library)
Clone ID VSI221
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10168-1
Original site URL
Representative seq. ID VSI221P
(Link to Original site)
Representative DNA sequence 
>VSI221 (VSI221Q) /CSM/VS/VSI2-A/VSI221Q.Seq.d/
CTTTATCAAGTCAGAATGTCTCAAAAACAAACTAAACCAGCTGCTGCCACCAAAGCTGCT
CCAGCCAACACCACCATTATCACCACCAATCGATGTCTCATCACCATCAAGAATTAAAAC
ATCAACACCTTTTGTACAACTTTTAACTGAAAAATGGTATCTATTCCAAATCAATGATAT
TCCAACCCCACAAAATGATAATGATATTGATAAAAATATTTTACCAATTATTAATGAACT
TTTAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAATAAAAAAAAAAAANG
NGTNGGGTAATACAATATTTTATTTTTATTTTCAAATTTTTTTAAAAAAAAAAXXXXXXX
XXXGGNTTTATCAAGTCAGAATGTTTCAAAAACAAACTAAACCAGNTGNTGCCACCAAAG
GTGNTCCAGCCAACNCCNCCATTATCNCCNCCAATCGATGNNTCATCNCCATCAAGAATT
NAAACATCNACNCCTTTTGGACAACTTTTAACTGAAAAATGGGATNTATTCCAAANCAAT
GATATTCCNACCCCCCAAAATGATAATGANATTGATAAAAANATTTTACCNATTATTAAT
GNACTTTTTAATAAATAAATAAATAAATAAATNAATNAATNAATNAATAATAAAAAAAAA
AAAAGAGTTGTGTAATACAATATTTATTTTATTT
sequence update 2001. 3.22
Translated Amino Acid sequence 
lssqnvsktn*tscchqscssqhhhyhhqsmshhhqelkhqhllynf*lkngiysksmif
qphkmimilikifyqllmnf*ink*ink*ink*IIKKKXXXGNTIFYFYFQIFLKKK---

---GFIKSECFKNKLNQXXPPKVXQPTPPLSPPIDXSSPSRIXTSTPFGQLLTEKWDXFQ
XNDIPTPQNDNXIDKXILPIINXLFNK*ink*xnxxxnnkkkkrvv*yniyfi
Translated Amino Acid sequence (All Frames) 
Frame A:
lyqvrmsqkqtkpaaatkaapanttiittnrclitikn*nintfcttfn*kmvsipnq*y
snptk***y**kyftny**tfk*ink*ink*ink**kkkxxxviqyfififkff*kk---

---GFIKSECFKNKLNQXXPPKVXQPTPPLSPPIDXSSPSRIXTSTPFGQLLTEKWDXFQ
XNDIPTPQNDNXIDKXILPIINXLFNK*ink*xnxxxnnkkkkrvv*yniyfi

Frame B:
fikseclknklnqllppkllqptpplsppidvsspsriktstpfvqlltekwylfqindi
ptpqndndidknilpiinellnk*ink*ink*innkkkkxvg*ynilflfsnffkkk---

---xlssqnvsktn*txxchqrxssqxxhyxxqsmxhxhqexkhxxlldnf*lkngxysk
xmifxppkmimxlikxfyxllmxflink*inkxxnxxiikkkkelcntifilf

Frame C:
lssqnvsktn*tscchqscssqhhhyhhqsmshhhqelkhqhllynf*lkngiysksmif
qphkmimilikifyqllmnf*ink*ink*ink*IIKKKXXXGNTIFYFYFQIFLKKK---

---xyqvrmfqkqtkpxxatkgxpanxxiixxnrxxixiknxnixxfwttfn*kmgxipx
q*ysxppk***x**kxftxy*xtf**ink*inxxxnx**kkkkscviqylfy
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI221 (VSI221Q) /CSM/VS/VSI2-A/VSI221Q.Seq.d/                        480   e-134
SSM253 (SSM253Q) /CSM/SS/SSM2-C/SSM253Q.Seq.d/                        353   2e-96
SFI570 (SFI570Q) /CSM/SF/SFI5-C/SFI570Q.Seq.d/                        347   1e-94
VFL737 (VFL737Q) /CSM/VF/VFL7-B/VFL737Q.Seq.d/                        315   5e-85
SSL759 (SSL759Q) /CSM/SS/SSL7-C/SSL759Q.Seq.d/                        278   1e-73
SSL758 (SSL758Q) /CSM/SS/SSL7-C/SSL758Q.Seq.d/                        192   5e-48
VSJ540 (VSJ540Q) /CSM/VS/VSJ5-B/VSJ540Q.Seq.d/                        127   2e-28
VSK384 (VSK384Q) /CSM/VS/VSK3-D/VSK384Q.Seq.d/                        117   2e-25
VSI829 (VSI829Q) /CSM/VS/VSI8-B/VSI829Q.Seq.d/                        113   4e-24
VSK343 (VSK343Q) /CSM/VS/VSK3-B/VSK343Q.Seq.d/                        109   6e-23
own update 2001.11.29
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU268342) Dictyostelium discoideum vegetative cDNA clone:VS...   391   e-120  2
(AU268343) Dictyostelium discoideum vegetative cDNA clone:VS...   361   e-105  2
(AU038995) Dictyostelium discoideum slug cDNA, clone SSM253.      264   8e-77  2
(BJ434598) Dictyostelium discoideum cDNA clone:ddv24j09, 3' ...   264   4e-66  1
(BJ402904) Dictyostelium discoideum cDNA clone:dds18k17, 3' ...   264   4e-66  1
(C93824) Dictyostelium discoideum slug cDNA, clone SSL759.        210   5e-50  1
(C93823) Dictyostelium discoideum slug cDNA, clone SSL758.        129   2e-25  1
(AU270080) Dictyostelium discoideum vegetative cDNA clone:VS...   127   6e-25  1
(AU271106) Dictyostelium discoideum vegetative cDNA clone:VS...   117   6e-22  1
(AU269296) Dictyostelium discoideum vegetative cDNA clone:VS...   113   9e-21  1
dna update 2009. 7. 5
Homology vs Protein 

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3019103
Number of Hits to DB: 567,126,073
protein update 2009. 3.24
PSORT 

 psg: 0.88  gvh: 0.33  alm: 0.49  top: 0.60  tms: 0.00  mit: 0.28  mip: 0.11
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: cytoskeletal
	  8.0 %: vacuolar
	  4.0 %: mitochondrial

>> prediction for VSI221 is cyt
5' end seq. ID VSI221F
5' end seq. 
>VSI221F.Seq
CTTTATCAAGTCAGAATGTCTCAAAAACAAACTAAACCAGCTGCTGCCACCAAAGCTGCT
CCAGCCAACACCACCATTATCACCACCAATCGATGTCTCATCACCATCAAGAATTAAAAC
ATCAACACCTTTTGTACAACTTTTAACTGAAAAATGGTATCTATTCCAAATCAATGATAT
TCCAACCCCACAAAATGATAATGATATTGATAAAAATATTTTACCAATTATTAATGAACT
TTTAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAATAAAAAAAAAAAANG
NGTNGGGTAATACAATATTTTATTTTTATTTTCAAATTTTTTTAAAAAAAAAA-------
---
Length of 5' end seq. 353
3' end seq. ID VSI221Z
3' end seq. 
>VSI221Z.Seq
----------GGNTTTATCAAGTCAGAATGTTTCAAAAACAAACTAAACCAGNTGNTGCC
ACCAAAGGTGNTCCAGCCAACNCCNCCATTATCNCCNCCAATCGATGNNTCATCNCCATC
AAGAATTNAAACATCNACNCCTTTTGGACAACTTTTAACTGAAAAATGGGATNTATTCCA
AANCAATGATATTCCNACCCCCCAAAATGATAATGANATTGATAAAAANATTTTACCNAT
TATTAATGNACTTTTTAATAAATAAATAAATAAATAAATNAATNAATNAATNAATAATAA
AAAAAAAAAAAGAGTTGTGTAATACAATATTTATTTTATTT
Length of 3' end seq. 331
Connected seq. ID VSI221P
Connected seq. 
>VSI221P.Seq
CTTTATCAAGTCAGAATGTCTCAAAAACAAACTAAACCAGCTGCTGCCACCAAAGCTGCT
CCAGCCAACACCACCATTATCACCACCAATCGATGTCTCATCACCATCAAGAATTAAAAC
ATCAACACCTTTTGTACAACTTTTAACTGAAAAATGGTATCTATTCCAAATCAATGATAT
TCCAACCCCACAAAATGATAATGATATTGATAAAAATATTTTACCAATTATTAATGAACT
TTTAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAATAAAAAAAAAAAANG
NGTNGGGTAATACAATATTTTATTTTTATTTTCAAATTTTTTTAAAAAAAAAA-------
---GGNTTTATCAAGTCAGAATGTTTCAAAAACAAACTAAACCAGNTGNTGCCACCAAAG
GTGNTCCAGCCAACNCCNCCATTATCNCCNCCAATCGATGNNTCATCNCCATCAAGAATT
NAAACATCNACNCCTTTTGGACAACTTTTAACTGAAAAATGGGATNTATTCCAAANCAAT
GATATTCCNACCCCCCAAAATGATAATGANATTGATAAAAANATTTTACCNATTATTAAT
GNACTTTTTAATAAATAAATAAATAAATAAATNAATNAATNAATNAATAATAAAAAAAAA
AAAAGAGTTGTGTAATACAATATTTATTTTATTT
Length of connected seq. 684
Full length Seq ID -
Full length Seq. -
Length of full length seq. -