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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSI215
Library VS
(Link to library)
Clone ID VSI215
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16226-1
Original site URL
Representative seq. ID VSI215P
(Link to Original site)
Representative DNA sequence 
>VSI215 (VSI215Q) /CSM/VS/VSI2-A/VSI215Q.Seq.d/
CGAAGNATGATGCTGAAGAACAAGAAGAAGACGAAGTCGAAGAAGAGGAAGAAGAACAAC
AAGAAGAAGAAGAAGGTGAAAATGATGAAGAATTAACTGAAGGAGCCGAAGAAGATTCCC
ACGAACACCCATTCGAAATTGAAGTCACACCAAAAGGTAACGCTTCCGGTAAACTCACTT
TTGGTTGTTATGCTTCACACGACGGTAACTACACTGTCTCTGGTTTCTACAAAGGTGGTT
TCGGTGAAACTGTAAACCCAGTCGATATTGGTGGAACTTCAATGGAATTCCAAGACAATA
TCTTATTAGTTTTACAACAATACGGTATCAATGAACGTTTATCTTTCTTCATCCACGATT
ATGTCCACAACAAGAAAATCAATGATTACGTTGAATCATTCGAAGCCTTAAAAGATTTTG
TTTCAAAAGAATAAATTAAATTTTAAATAAAAAAAATAAAAAAAAAAAATTNAAAAAAAA
AATTAAGGTNNNAAAAAAAXXXXXXXXXXGGNTTCGGNNAAACTNTAAACCCNGNCGAAA
TTGGNGGAACTTCAANGGAANTCCAANACAAAATNTTNTTAGTTTTACAACAAAACGGGA
ACAANGAACGTTTATCNTTNTTCANCCCCGATTANGGCCCCAACAAAAAAAACA
sequence update 2001. 3.22
Translated Amino Acid sequence 
ex*c*rtrrrrsrrrgrrttrrrrr*k**rin*rsrrrfprtpirn*shtkr*rfr*thf
wllcftrr*lhclwflqrwfr*nckpsrywwnfngiprqylisfttiryq*tfiflhprl
cpqqenq*lr*iirslkrfcfkrin*ILNKKNKKKKXKKKIKVXKK---

---GFGXTXNPXEIGGTSXEXQXKXXLVLQQNGNXERLSXFXPDXGPNKKN
Translated Amino Acid sequence (All Frames) 
Frame A:
rxmmlknkkktkskkrkknnkkkkkvkmmkn*lkepkkiptnthsklkshqkvtlpvnsl
lvvmlhttvttlslvstkvvsvkl*tqsilvelqwnsktisy*fynntvsmnvylsssti
msttrksmitlnhskp*kilfqknklnfk*kk*kkkixkkn*gxkk---

---GFGXTXNPXEIGGTSXEXQXKXXLVLQQNGNXERLSXFXPDXGPNKKN

Frame B:
ex*c*rtrrrrsrrrgrrttrrrrr*k**rin*rsrrrfprtpirn*shtkr*rfr*thf
wllcftrr*lhclwflqrwfr*nckpsrywwnfngiprqylisfttiryq*tfiflhprl
cpqqenq*lr*iirslkrfcfkrin*ILNKKNKKKKXKKKIKVXKK---

---xsxkl*txxklxelqxxsxtkxx*fynktgtxnvyxxsxpixaptkkt

Frame C:
kxdaeeqeedeveeeeeeqqeeeegendeeltegaeedshehpfeievtpkgnasgkltf
gcyashdgnytvsgfykggfgetvnpvdiggtsmefqdnillvlqqyginerlsffihdy
vhnkkindyvesfealkdfvske*ikf*ikkikkknxkkklrxxk---

---xrxnxkpxrnwxnfxgxpxqnxxsfttkreqxtfixxxprlxpqqkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI215 (VSI215Q) /CSM/VS/VSI2-A/VSI215Q.Seq.d/                        706   0.0
VSF150 (VSF150Q) /CSM/VS/VSF1-C/VSF150Q.Seq.d/                        706   0.0
VSB674 (VSB674Q) /CSM/VS/VSB6-D/VSB674Q.Seq.d/                        706   0.0
VSE558 (VSE558Q) /CSM/VS/VSE5-C/VSE558Q.Seq.d/                        692   0.0
FC-BG07 (FC-BG07Q) /CSM/FC/FC-BG/FC-BG07Q.Seq.d/                      690   0.0
FC-AW06 (FC-AW06Q) /CSM/FC/FC-AW/FC-AW06Q.Seq.d/                      690   0.0
FC-336 (FC-336Q) /CSM/CG/CG-FC/FC-336Q.Seq.d/                         690   0.0
CHK474 (CHK474Q) /CSM/CH/CHK4-D/CHK474Q.Seq.d/                        650   0.0
VFJ605 (VFJ605Q) /CSM/VF/VFJ6-A/VFJ605Q.Seq.d/                        561   e-159
VFL352 (VFL352Q) /CSM/VF/VFL3-C/VFL352Q.Seq.d/                        353   2e-96
own update 2003. 1.10
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU268332) Dictyostelium discoideum vegetative cDNA clone:VS...   613   e-171  1
(AU264783) Dictyostelium discoideum vegetative cDNA clone:VS...   613   e-171  1
(AU262173) Dictyostelium discoideum vegetative cDNA clone:VS...   613   e-171  1
(AU263198) Dictyostelium discoideum vegetative cDNA clone:VS...   599   e-167  1
(AU284653) Dictyostelium discoideum gamete cDNA clone:FC-BG0...   597   e-166  1
(C25722) Dictyostelium discoideum gamete cDNA, clone FC-AW06.     597   e-166  1
(BJ381823) Dictyostelium discoideum cDNA clone:ddc42d20, 3' ...   547   e-161  2
(BJ432868) Dictyostelium discoideum cDNA clone:ddv20i02, 3' ...   505   e-142  2
(BJ433999) Dictyostelium discoideum cDNA clone:ddv23h13, 3' ...   325   1e-84  1
(AU268333) Dictyostelium discoideum vegetative cDNA clone:VS...   167   7e-37  1
dna update 2009. 7. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY086223_1(AY086223|pid:none) Arabidopsis thaliana clone 22656 m...    40   0.056
AP004702_16(AP004702|pid:none) Oryza sativa Japonica Group genom...    36   0.81
CP000721_1484(CP000721|pid:none) Clostridium beijerinckii NCIMB ...    36   0.81
(P40513) RecName: Full=Mitochondrial acidic protein MAM33; Flags...    35   1.4
CR380959_553(CR380959|pid:none) Candida glabrata strain CBS138 c...    35   1.4
AY557860_1(AY557860|pid:none) Saccharomyces cerevisiae clone FLH...    35   1.4
CP001348_1465(CP001348|pid:none) Clostridium cellulolyticum H10,...    35   1.8
EU960105_1(EU960105|pid:none) Zea mays clone 221712 mitochondria...    33   5.2
CR382127_70(CR382127|pid:none) Yarrowia lipolytica strain CLIB12...    33   6.8
BC138874_1(BC138874|pid:none) Mus musculus poly (ADP-ribose) pol...    33   8.9
protein update 2009. 3.24
PSORT 

 psg: 0.45  gvh: 0.41  alm: 0.63  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.23  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: mitochondrial

>> prediction for VSI215 is nuc
5' end seq. ID VSI215F
5' end seq. 
>VSI215F.Seq
CGAAGNATGATGCTGAAGAACAAGAAGAAGACGAAGTCGAAGAAGAGGAAGAAGAACAAC
AAGAAGAAGAAGAAGGTGAAAATGATGAAGAATTAACTGAAGGAGCCGAAGAAGATTCCC
ACGAACACCCATTCGAAATTGAAGTCACACCAAAAGGTAACGCTTCCGGTAAACTCACTT
TTGGTTGTTATGCTTCACACGACGGTAACTACACTGTCTCTGGTTTCTACAAAGGTGGTT
TCGGTGAAACTGTAAACCCAGTCGATATTGGTGGAACTTCAATGGAATTCCAAGACAATA
TCTTATTAGTTTTACAACAATACGGTATCAATGAACGTTTATCTTTCTTCATCCACGATT
ATGTCCACAACAAGAAAATCAATGATTACGTTGAATCATTCGAAGCCTTAAAAGATTTTG
TTTCAAAAGAATAAATTAAATTTTAAATAAAAAAAATAAAAAAAAAAAATTNAAAAAAAA
AATTAAGGTNNNAAAAAAA----------
Length of 5' end seq. 499
3' end seq. ID VSI215Z
3' end seq. 
>VSI215Z.Seq
----------GGNTTCGGNNAAACTNTAAACCCNGNCGAAATTGGNGGAACTTCAANGGA
ANTCCAANACAAAATNTTNTTAGTTTTACAACAAAACGGGAACAANGAACGTTTATCNTT
NTTCANCCCCGATTANGGCCCCAACAAAAAAAACA
Length of 3' end seq. 145
Connected seq. ID VSI215P
Connected seq. 
>VSI215P.Seq
CGAAGNATGATGCTGAAGAACAAGAAGAAGACGAAGTCGAAGAAGAGGAAGAAGAACAAC
AAGAAGAAGAAGAAGGTGAAAATGATGAAGAATTAACTGAAGGAGCCGAAGAAGATTCCC
ACGAACACCCATTCGAAATTGAAGTCACACCAAAAGGTAACGCTTCCGGTAAACTCACTT
TTGGTTGTTATGCTTCACACGACGGTAACTACACTGTCTCTGGTTTCTACAAAGGTGGTT
TCGGTGAAACTGTAAACCCAGTCGATATTGGTGGAACTTCAATGGAATTCCAAGACAATA
TCTTATTAGTTTTACAACAATACGGTATCAATGAACGTTTATCTTTCTTCATCCACGATT
ATGTCCACAACAAGAAAATCAATGATTACGTTGAATCATTCGAAGCCTTAAAAGATTTTG
TTTCAAAAGAATAAATTAAATTTTAAATAAAAAAAATAAAAAAAAAAAATTNAAAAAAAA
AATTAAGGTNNNAAAAAAA----------GGNTTCGGNNAAACTNTAAACCCNGNCGAAA
TTGGNGGAACTTCAANGGAANTCCAANACAAAATNTTNTTAGTTTTACAACAAAACGGGA
ACAANGAACGTTTATCNTTNTTCANCCCCGATTANGGCCCCAACAAAAAAAACA
Length of connected seq. 644
Full length Seq ID -
Full length Seq. -
Length of full length seq. -