NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSI194
Library VS
(Link to library)
Clone ID VSI194
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U06213-1
Original site URL
Representative seq. ID VSI194P
(Link to Original site)
Representative DNA sequence 
>VSI194 (VSI194Q) /CSM/VS/VSI1-D/VSI194Q.Seq.d/
GTCATTATCTGGTATGGTTAGAATTGAATTACCACATATTAATGTATTATCAAAGATGGA
TTTAATTGAACAAAATGGACCATTAGATTTTAATTTAGATTTTTATACAGATGTATTGGA
TTTGAAATATTTAGATGCATTCCTTGATAAAGATCCACGTTTAAAAAAGTATAGTAAATT
GAATAAAGCGATTGCAGGTGTTATAGAAGATTTTAGTTTGGTTAGTTTCATACCATTGAA
TATAATGGACAAGAAATCTGTTGCAAACCTAATTGCATCAATTGATAAAAGTAATGGTTA
TATATATGGTTCACTCGATACAAATACTGCCATTTTAGAGATTCAAGAAAGAGAAACTCA
ATGGAATTTCGATAAATATCAAGAAACTCAAGAGAAATATTATAAATCATACGAAGATGA
TGATGTTATCTTTGAAAATGATCAAGATGAAGATTATGATGAATTTAGTAAATATTTAAA
TAGATAAATAAATAATAATAAAATAATAATAATAATAAAAAAAAAAXXXXXXXXXXGTCA
TTATCTGGTATGGTTAGAATTGAATTACCACATATTAATGTATTATCAAAGATGGATTTA
ATTGAACAAAATGGACCATTAGATTTTAATTTAGATTTTTATACAGATGTATTGGATTTG
AAATATTTAGATGCATTCCTTGATAAAGATCCACGTTTAAAAAAGTATAGTAAATTGAAT
AAAGCGATTGCAGGTGTTATAGAAGATTTTAGTTTGGTTAGTTTCATACCATTGAATATA
ATGGACAAGAAATCTGTTGCAAACCTAATTGCATCAATTGATAAAAGTAATGGTTATATA
TATGGTTCACTCGATACAAATACTGCCATTTTAGAGATTCAAGAAAGAGAAACTCAATGG
AATTTCGATAAATATCAAGAAACTCAAGAGAAATATTATAAATCATACGAAGATGATGAT
GTTATCTTTGAAAATGATCAAGATGAAGATTATGATGAATTTAGTAAATATTTAAATAGA
TAAATAAATAATAATAAAATA
sequence update 2001. 3.22
Translated Amino Acid sequence 
slsgmvrielphinvlskmdlieqngpldfnldfytdvldlkyldafldkdprlkkyskl
nkaiagviedfslvsfiplnimdkksvanliasidksngyiygsldtntaileiqeretq
wnfdkyqetqekyyksyedddvifendqdedydefskylnr*INNNKIIIIIKKK---

---SLSGMVRIELPHINVLSKMDLIEQNGPLDFNLDFYTDVLDLKYLDAFLDKDPRLKKY
SKLNKAIAGVIEDFSLVSFIPLNIMDKKSVANLIASIDKSNGYIYGSLDTNTAILEIQER
ETQWNFDKYQETQEKYYKSYEDDDVIFENDQDEDYDEFSKYLNR*innnki
Translated Amino Acid sequence (All Frames) 
Frame A:
viiwyg*n*itty*ciikdgfn*tkwtirf*frflyrcigfeifrcip**rstfkkv**i
e*sdcrcyrrf*fg*fhtieyngqeicckpncin**k*wlyiwftrykychfrdsrkrns
mefr*isrnsreil*iirr**cyl*k*sr*rl**i**ifk*ink***nnnnnkkk---

---viiwyg*n*itty*ciikdgfn*tkwtirf*frflyrcigfeifrcip**rstfkkv
**ie*sdcrcyrrf*fg*fhtieyngqeicckpncin**k*wlyiwftrykychfrdsrk
rnsmefr*isrnsreil*iirr**cyl*k*sr*rl**i**ifk*ink***n

Frame B:
slsgmvrielphinvlskmdlieqngpldfnldfytdvldlkyldafldkdprlkkyskl
nkaiagviedfslvsfiplnimdkksvanliasidksngyiygsldtntaileiqeretq
wnfdkyqetqekyyksyedddvifendqdedydefskylnr*INNNKIIIIIKKK---

---SLSGMVRIELPHINVLSKMDLIEQNGPLDFNLDFYTDVLDLKYLDAFLDKDPRLKKY
SKLNKAIAGVIEDFSLVSFIPLNIMDKKSVANLIASIDKSNGYIYGSLDTNTAILEIQER
ETQWNFDKYQETQEKYYKSYEDDDVIFENDQDEDYDEFSKYLNR*innnki

Frame C:
hylvwlelnyhilmyyqrwi*lnkmdh*ili*ifiqmywi*ni*mhslikihv*ksivn*
ikrlqvl*kilvwlvsyh*i*wtrnllqt*lhqlikvmviymvhsiqilpf*rfkkekln
gisinikklkrniinhtkmmmlslkmikmkimmnlvni*idk*iiik*****kk---

---hylvwlelnyhilmyyqrwi*lnkmdh*ili*ifiqmywi*ni*mhslikihv*ksi
vn*ikrlqvl*kilvwlvsyh*i*wtrnllqt*lhqlikvmviymvhsiqilpf*rfkke
klngisinikklkrniinhtkmmmlslkmikmkimmnlvni*idk*iiik
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI194 (VSI194Q) /CSM/VS/VSI1-D/VSI194Q.Seq.d/                        928   0.0
VSA134 (VSA134Q) /CSM/VS/VSA1-B/VSA134Q.Seq.d/                        928   0.0
VSB839 (VSB839Q) /CSM/VS/VSB8-B/VSB839Q.Seq.d/                         36   1.0
VHO303 (VHO303Q) /CSM/VH/VHO3-A/VHO303Q.Seq.d/                         36   1.0
VFM552 (VFM552Q) /CSM/VF/VFM5-C/VFM552Q.Seq.d/                         36   1.0
VFH869 (VFH869Q) /CSM/VF/VFH8-C/VFH869Q.Seq.d/                         36   1.0
VFF596 (VFF596Q) /CSM/VF/VFF5-D/VFF596Q.Seq.d/                         36   1.0
VFA265 (VFA265Q) /CSM/VF/VFA2-C/VFA265Q.Seq.d/                         36   1.0
SSI452 (SSI452Q) /CSM/SS/SSI4-C/SSI452Q.Seq.d/                         36   1.0
SSG592 (SSG592Q) /CSM/SS/SSG5-D/SSG592Q.Seq.d/                         36   1.0
own update 2003. 1.10
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU268302) Dictyostelium discoideum vegetative cDNA clone:VS...   928   0.0    1
(AU268301) Dictyostelium discoideum vegetative cDNA clone:VS...   928   0.0    1
(AU261301) Dictyostelium discoideum vegetative cDNA clone:VS...   928   0.0    1
(EJ054497) 1095456018741 Global-Ocean-Sampling_GS-26-01-01-1...    36   0.006  3
(DR071219) RTDK1_18_A03.g1_A029 Roots, dark Pinus taeda cDNA...    36   0.007  3
(FD453503) EGGAO04TR Haematobia irritans eggs Haematobia irr...    54   0.011  1
(FD453502) EGGAO04TF Haematobia irritans eggs Haematobia irr...    54   0.011  1
(AZ546009) ENTFW53TF Entamoeba histolytica Sheared DNA Entam...    32   0.026  4
(AZ550256) ENTEV58TR Entamoeba histolytica Sheared DNA Entam...    32   0.031  4
(EJ143505) 1092343672350 Global-Ocean-Sampling_GS-27-01-01-1...    42   0.049  2
dna update 2009. 7. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54TE7) RecName: Full=GPN-loop GTPase 2 homolog; AltName: Full=...   325   1e-87
EU968965_1(EU968965|pid:none) Zea mays clone 325263 ATP binding ...   146   1e-33
EU962167_1(EU962167|pid:none) Zea mays clone 240711 ATP binding ...   146   1e-33
BT034798_1(BT034798|pid:none) Zea mays full-length cDNA clone ZM...   146   1e-33
AK121253_1(AK121253|pid:none) Oryza sativa Japonica Group cDNA c...   145   2e-33
AK221541_1(AK221541|pid:none) Arabidopsis thaliana mRNA for puta...   143   1e-32
AM475696_5(AM475696|pid:none) Vitis vinifera contig VV78X042996....   139   2e-31
BC152270_1(BC152270|pid:none) Danio rerio GPN-loop GTPase 2, mRN...   137   8e-31
(Q6PUR6) RecName: Full=GPN-loop GTPase 2; AltName: Full=ATP-bind...   134   4e-30
AY335563_1(AY335563|pid:none) Synthetic construct Homo sapiens h...   126   1e-27
protein update 2009. 3.24
PSORT 

 psg: 0.69  gvh: 0.27  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.05
 nuc: 0.06  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: cytoskeletal
	  8.0 %: vacuolar
	  4.0 %: extracellular, including cell wall
	  4.0 %: mitochondrial
	  4.0 %: plasma membrane

>> prediction for VSI194 is cyt
5' end seq. ID VSI194F
5' end seq. 
>VSI194F.Seq
GTCATTATCTGGTATGGTTAGAATTGAATTACCACATATTAATGTATTATCAAAGATGGA
TTTAATTGAACAAAATGGACCATTAGATTTTAATTTAGATTTTTATACAGATGTATTGGA
TTTGAAATATTTAGATGCATTCCTTGATAAAGATCCACGTTTAAAAAAGTATAGTAAATT
GAATAAAGCGATTGCAGGTGTTATAGAAGATTTTAGTTTGGTTAGTTTCATACCATTGAA
TATAATGGACAAGAAATCTGTTGCAAACCTAATTGCATCAATTGATAAAAGTAATGGTTA
TATATATGGTTCACTCGATACAAATACTGCCATTTTAGAGATTCAAGAAAGAGAAACTCA
ATGGAATTTCGATAAATATCAAGAAACTCAAGAGAAATATTATAAATCATACGAAGATGA
TGATGTTATCTTTGAAAATGATCAAGATGAAGATTATGATGAATTTAGTAAATATTTAAA
TAGATAAATAAATAATAATAAAATAATAATAATAATAAAAAAAAAA----------
Length of 5' end seq. 526
3' end seq. ID VSI194Z
3' end seq. 
>VSI194Z.Seq
----------GTCATTATCTGGTATGGTTAGAATTGAATTACCACATATTAATGTATTAT
CAAAGATGGATTTAATTGAACAAAATGGACCATTAGATTTTAATTTAGATTTTTATACAG
ATGTATTGGATTTGAAATATTTAGATGCATTCCTTGATAAAGATCCACGTTTAAAAAAGT
ATAGTAAATTGAATAAAGCGATTGCAGGTGTTATAGAAGATTTTAGTTTGGTTAGTTTCA
TACCATTGAATATAATGGACAAGAAATCTGTTGCAAACCTAATTGCATCAATTGATAAAA
GTAATGGTTATATATATGGTTCACTCGATACAAATACTGCCATTTTAGAGATTCAAGAAA
GAGAAACTCAATGGAATTTCGATAAATATCAAGAAACTCAAGAGAAATATTATAAATCAT
ACGAAGATGATGATGTTATCTTTGAAAATGATCAAGATGAAGATTATGATGAATTTAGTA
AATATTTAAATAGATAAATAAATAATAATAAAATA
Length of 3' end seq. 505
Connected seq. ID VSI194P
Connected seq. 
>VSI194P.Seq
GTCATTATCTGGTATGGTTAGAATTGAATTACCACATATTAATGTATTATCAAAGATGGA
TTTAATTGAACAAAATGGACCATTAGATTTTAATTTAGATTTTTATACAGATGTATTGGA
TTTGAAATATTTAGATGCATTCCTTGATAAAGATCCACGTTTAAAAAAGTATAGTAAATT
GAATAAAGCGATTGCAGGTGTTATAGAAGATTTTAGTTTGGTTAGTTTCATACCATTGAA
TATAATGGACAAGAAATCTGTTGCAAACCTAATTGCATCAATTGATAAAAGTAATGGTTA
TATATATGGTTCACTCGATACAAATACTGCCATTTTAGAGATTCAAGAAAGAGAAACTCA
ATGGAATTTCGATAAATATCAAGAAACTCAAGAGAAATATTATAAATCATACGAAGATGA
TGATGTTATCTTTGAAAATGATCAAGATGAAGATTATGATGAATTTAGTAAATATTTAAA
TAGATAAATAAATAATAATAAAATAATAATAATAATAAAAAAAAAA----------GTCA
TTATCTGGTATGGTTAGAATTGAATTACCACATATTAATGTATTATCAAAGATGGATTTA
ATTGAACAAAATGGACCATTAGATTTTAATTTAGATTTTTATACAGATGTATTGGATTTG
AAATATTTAGATGCATTCCTTGATAAAGATCCACGTTTAAAAAAGTATAGTAAATTGAAT
AAAGCGATTGCAGGTGTTATAGAAGATTTTAGTTTGGTTAGTTTCATACCATTGAATATA
ATGGACAAGAAATCTGTTGCAAACCTAATTGCATCAATTGATAAAAGTAATGGTTATATA
TATGGTTCACTCGATACAAATACTGCCATTTTAGAGATTCAAGAAAGAGAAACTCAATGG
AATTTCGATAAATATCAAGAAACTCAAGAGAAATATTATAAATCATACGAAGATGATGAT
GTTATCTTTGAAAATGATCAAGATGAAGATTATGATGAATTTAGTAAATATTTAAATAGA
TAAATAAATAATAATAAAATA
Length of connected seq. 1031
Full length Seq ID -
Full length Seq. -
Length of full length seq. -