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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSI174
Library VS
(Link to library)
Clone ID VSI174
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16583-1
Original site URL
Representative seq. ID VSI174P
(Link to Original site)
Representative DNA sequence 
>VSI174 (VSI174Q) /CSM/VS/VSI1-D/VSI174Q.Seq.d/
GAAAGAAAGAAGTCAAAGCCCCAAAAGTTGTTGAAGCTTTATCAGCCAAAATCCCATGTG
GTCCAAGTGAATTAGTCTTTGGTGTTGCCCATATCTTCGCCTCATTCAATGATACCTTTG
TCCACGTCACTGATTTATCCTCAAAAGAAACCATTGTTCGTGTTACTGGTGGTATGAAAG
TTAAAACCGATCGTGATGAATCTTCACCATACGCTGCCACCATGGCCGCTCAAGATGTCG
CCCTCCGTTGTAAAGAACTCGGTGTCACTGCTCTCCATATTAAATTAAGAGCTACCGGTG
GTAATGGTTCAAAGACCCCAGGTCCAGGTGCTCAATCTGCCCTCAGAGCTTTAGCTCGTT
CAGGTATGAGAATTGGTCGTATTGAAGATGTTACCCCAATTCCAACTGATTCAACCAGAA
GAAAGAGCGGTCATCGTGGTAGACGTTTATAAACATTTGGATCTCATCATTTCAAGTGCT
TTTTTAATAATAATACTACAACCTTTTTAATTTTAAAAAAAACCAAAAAAAAAACAATTT
GTTTTAATACAACAACAACTXXXXXXXXXXCCACGNGTCCGCCCACGCGTTCCGGAAAGA
AAGAAGTCAAAGCCCCAAAAGTTGTTNAAGCTTTATCAGCCAAAATCCCATGTGGTCCAA
GTGAATTAGTNTTTGGTGTTGCCCATATCTTCGCCTCATTCAATGATACCTTTGTCCACG
TCACTGATTTATCCTCAAAANAAACCATTGTTCGTGTTACTGGTGGTATGAAAGTTAAAA
CCGATCGTGATGAATNTTCACCATACGCTGCCACCATGGCCGCTCAAGATGTCGCCCTCC
GTTGTAAAGAACTCGGTGTCACTGCTCTCCATATTAAATTAANAGCTACCGGTGGTAATG
GTTCAAAGACCCCAGGTCCAGGTGCTCAATCTGCCCTCAGAGCTTTAGCTCGTTCAGGTA
TGAGAATTGGTCGTATTGAAGATGTTACCCCAATTCCAACTGATTCAACCAGAAGAAAGA
GCGGTCATCGTGGTAGACGTTTATAAACATTTGGATCTCATCATTCAAGTGCTTTTTTAA
TAATAATACTACAACCTTTTTAATTTTAAAAAAAACCAAAAAAAAAACAATTTGTTTTAA
TACAACAACAACTCTAATAAAATAGGTA
sequence update 2001. 3.22
Translated Amino Acid sequence 
kkevkapkvvealsakipcgpselvfgvahifasfndtfvhvtdlssketivrvtggmkv
ktdrdesspyaatmaaqdvalrckelgvtalhiklratggngsktpgpgaqsalralars
gmrigriedvtpiptdstrrksghrgrrl*TFGSHHFKCFFNNNTTTFLILKKTKKKTIC
FNTTTT---

---TXPPTRSGKKEVKAPKVVXALSAKIPCGPSELVFGVAHIFASFNDTFVHVTDLSSKX
TIVRVTGGMKVKTDRDEXSPYAATMAAQDVALRCKELGVTALHIKLXATGGNGSKTPGPG
AQSALRALARSGMRIGRIEDVTPIPTDSTRRKSGHRGRRL*tfgshhssafliiilqpf*
f*kkpkkkqfvliqqql**nr
Translated Amino Acid sequence (All Frames) 
Frame A:
erkkskpqkllklyqpkshvvqvn*slvlpissphsmiplstsliypqkkplfvllvv*k
lkpivmnlhhtlppwplkmspsvvknsvsllsiln*elpvvmvqrpqvqvlnlpsel*lv
qv*elvvlkmlpqfqliqpeeravivvdvykhldliissafliiilqpf*f*kkpkkkqf
vliqqq---

---prvrprvperkkskpqklxklyqpkshvvqvn*xlvlpissphsmiplstsliypqx
kplfvllvv*klkpivmnxhhtlppwplkmspsvvknsvsllsiln*xlpvvmvqrpqvq
vlnlpsel*lvqv*elvvlkmlpqfqliqpeeravivvdvykhldliiqvlf***yynlf
nfkknqkknnlf*ynnnsnkig

Frame B:
kersqspksc*sfisqnpmwsk*islwccpylrliq*ylcprh*filkrnhcscywwyes
*nrs**iftirchhgrsrcrppl*rtrchcspy*iksyrw*wfkdprsrcsicpqsfssf
ryenwsy*rcypnsn*fnqkkerssw*tfiniwissfqvlf***yynlfnfkknqkknnl
f*ynnn---

---hxsahafrkersqspkscxsfisqnpmwsk*isxwccpylrliq*ylcprh*filkx
nhcscywwyes*nrs**xftirchhgrsrcrppl*rtrchcspy*ixsyrw*wfkdprsr
csicpqsfssfryenwsy*rcypnsn*fnqkkerssw*tfiniwissfkcffnnntttfl
ilkktkkkticfnttttlik*v

Frame C:
kkevkapkvvealsakipcgpselvfgvahifasfndtfvhvtdlssketivrvtggmkv
ktdrdesspyaatmaaqdvalrckelgvtalhiklratggngsktpgpgaqsalralars
gmrigriedvtpiptdstrrksghrgrrl*TFGSHHFKCFFNNNTTTFLILKKTKKKTIC
FNTTTT---

---TXPPTRSGKKEVKAPKVVXALSAKIPCGPSELVFGVAHIFASFNDTFVHVTDLSSKX
TIVRVTGGMKVKTDRDEXSPYAATMAAQDVALRCKELGVTALHIKLXATGGNGSKTPGPG
AQSALRALARSGMRIGRIEDVTPIPTDSTRRKSGHRGRRL*tfgshhssafliiilqpf*
f*kkpkkkqfvliqqql**nr
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI174 (VSI174Q) /CSM/VS/VSI1-D/VSI174Q.Seq.d/                       1041   0.0
VSJ643 (VSJ643Q) /CSM/VS/VSJ6-B/VSJ643Q.Seq.d/                       1019   0.0
VSJ390 (VSJ390Q) /CSM/VS/VSJ3-D/VSJ390Q.Seq.d/                       1019   0.0
VSG611 (VSG611Q) /CSM/VS/VSG6-A/VSG611Q.Seq.d/                       1019   0.0
VSD704 (VSD704Q) /CSM/VS/VSD7-A/VSD704Q.Seq.d/                       1019   0.0
VSK147 (VSK147Q) /CSM/VS/VSK1-B/VSK147Q.Seq.d/                       1003   0.0
VSH759 (VSH759Q) /CSM/VS/VSH7-C/VSH759Q.Seq.d/                       1003   0.0
VSH736 (VSH736Q) /CSM/VS/VSH7-B/VSH736Q.Seq.d/                       1003   0.0
VSH639 (VSH639Q) /CSM/VS/VSH6-B/VSH639Q.Seq.d/                       1003   0.0
VSH511 (VSH511Q) /CSM/VS/VSH5-A/VSH511Q.Seq.d/                       1003   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU268268) Dictyostelium discoideum vegetative cDNA clone:VS...  1029   0.0    2
(AU270246) Dictyostelium discoideum vegetative cDNA clone:VS...  1019   0.0    2
(AU284713) Dictyostelium discoideum gamete cDNA clone:FC-BI0...   991   0.0    2
(C23683) Dictyostelium discoideum gamete cDNA, clone FC-AE08.     991   0.0    2
(AU284581) Dictyostelium discoideum gamete cDNA clone:FC-BD2...   991   0.0    2
(AU268267) Dictyostelium discoideum vegetative cDNA clone:VS...  1019   0.0    2
(AU267483) Dictyostelium discoideum vegetative cDNA clone:VS...   991   0.0    2
(AU264088) Dictyostelium discoideum vegetative cDNA clone:VS...   985   0.0    2
(AU267911) Dictyostelium discoideum vegetative cDNA clone:VS...   979   0.0    2
(AU267280) Dictyostelium discoideum vegetative cDNA clone:VS...   973   0.0    3
dna update 2009. 7. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54EG3) RecName: Full=40S ribosomal protein S14;                     272   2e-71
EF677282_1(EF677282|pid:none) Picea sitchensis clone WS02764_L13...   227   7e-58
EU960087_1(EU960087|pid:none) Zea mays clone 221629 40S ribosoma...   227   7e-58
(P19950) RecName: Full=40S ribosomal protein S14; AltName: Full=...   227   7e-58
BT034561_1(BT034561|pid:none) Zea mays full-length cDNA clone ZM...   226   1e-57
EU285011_1(EU285011|pid:none) TSA: Elaeis guineensis EgEfMPOB001...   226   1e-57
EU976524_1(EU976524|pid:none) Zea mays clone 896801 40S ribosoma...   226   1e-57
EF676341_1(EF676341|pid:none) Picea sitchensis clone WS02732_D01...   226   1e-57
AK058907_1(AK058907|pid:none) Oryza sativa Japonica Group cDNA c...   225   2e-57
EU976643_1(EU976643|pid:none) Zea mays clone 986007 40S ribosoma...   225   2e-57
protein update 2009. 3.24
PSORT 

 psg: 0.87  gvh: 0.43  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.45  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.50  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 88.0 %: cytoplasmic
	 12.0 %: mitochondrial

>> prediction for VSI174 is cyt
5' end seq. ID VSI174F
5' end seq. 
>VSI174F.Seq
GAAAGAAAGAAGTCAAAGCCCCAAAAGTTGTTGAAGCTTTATCAGCCAAAATCCCATGTG
GTCCAAGTGAATTAGTCTTTGGTGTTGCCCATATCTTCGCCTCATTCAATGATACCTTTG
TCCACGTCACTGATTTATCCTCAAAAGAAACCATTGTTCGTGTTACTGGTGGTATGAAAG
TTAAAACCGATCGTGATGAATCTTCACCATACGCTGCCACCATGGCCGCTCAAGATGTCG
CCCTCCGTTGTAAAGAACTCGGTGTCACTGCTCTCCATATTAAATTAAGAGCTACCGGTG
GTAATGGTTCAAAGACCCCAGGTCCAGGTGCTCAATCTGCCCTCAGAGCTTTAGCTCGTT
CAGGTATGAGAATTGGTCGTATTGAAGATGTTACCCCAATTCCAACTGATTCAACCAGAA
GAAAGAGCGGTCATCGTGGTAGACGTTTATAAACATTTGGATCTCATCATTTCAAGTGCT
TTTTTAATAATAATACTACAACCTTTTTAATTTTAAAAAAAACCAAAAAAAAAACAATTT
GTTTTAATACAACAACAACT----------
Length of 5' end seq. 560
3' end seq. ID VSI174Z
3' end seq. 
>VSI174Z.Seq
----------CCACGNGTCCGCCCACGCGTTCCGGAAAGAAAGAAGTCAAAGCCCCAAAA
GTTGTTNAAGCTTTATCAGCCAAAATCCCATGTGGTCCAAGTGAATTAGTNTTTGGTGTT
GCCCATATCTTCGCCTCATTCAATGATACCTTTGTCCACGTCACTGATTTATCCTCAAAA
NAAACCATTGTTCGTGTTACTGGTGGTATGAAAGTTAAAACCGATCGTGATGAATNTTCA
CCATACGCTGCCACCATGGCCGCTCAAGATGTCGCCCTCCGTTGTAAAGAACTCGGTGTC
ACTGCTCTCCATATTAAATTAANAGCTACCGGTGGTAATGGTTCAAAGACCCCAGGTCCA
GGTGCTCAATCTGCCCTCAGAGCTTTAGCTCGTTCAGGTATGAGAATTGGTCGTATTGAA
GATGTTACCCCAATTCCAACTGATTCAACCAGAAGAAAGAGCGGTCATCGTGGTAGACGT
TTATAAACATTTGGATCTCATCATTCAAGTGCTTTTTTAATAATAATACTACAACCTTTT
TAATTTTAAAAAAAACCAAAAAAAAAACAATTTGTTTTAATACAACAACAACTCTAATAA
AATAGGTA
Length of 3' end seq. 598
Connected seq. ID VSI174P
Connected seq. 
>VSI174P.Seq
GAAAGAAAGAAGTCAAAGCCCCAAAAGTTGTTGAAGCTTTATCAGCCAAAATCCCATGTG
GTCCAAGTGAATTAGTCTTTGGTGTTGCCCATATCTTCGCCTCATTCAATGATACCTTTG
TCCACGTCACTGATTTATCCTCAAAAGAAACCATTGTTCGTGTTACTGGTGGTATGAAAG
TTAAAACCGATCGTGATGAATCTTCACCATACGCTGCCACCATGGCCGCTCAAGATGTCG
CCCTCCGTTGTAAAGAACTCGGTGTCACTGCTCTCCATATTAAATTAAGAGCTACCGGTG
GTAATGGTTCAAAGACCCCAGGTCCAGGTGCTCAATCTGCCCTCAGAGCTTTAGCTCGTT
CAGGTATGAGAATTGGTCGTATTGAAGATGTTACCCCAATTCCAACTGATTCAACCAGAA
GAAAGAGCGGTCATCGTGGTAGACGTTTATAAACATTTGGATCTCATCATTTCAAGTGCT
TTTTTAATAATAATACTACAACCTTTTTAATTTTAAAAAAAACCAAAAAAAAAACAATTT
GTTTTAATACAACAACAACT----------CCACGNGTCCGCCCACGCGTTCCGGAAAGA
AAGAAGTCAAAGCCCCAAAAGTTGTTNAAGCTTTATCAGCCAAAATCCCATGTGGTCCAA
GTGAATTAGTNTTTGGTGTTGCCCATATCTTCGCCTCATTCAATGATACCTTTGTCCACG
TCACTGATTTATCCTCAAAANAAACCATTGTTCGTGTTACTGGTGGTATGAAAGTTAAAA
CCGATCGTGATGAATNTTCACCATACGCTGCCACCATGGCCGCTCAAGATGTCGCCCTCC
GTTGTAAAGAACTCGGTGTCACTGCTCTCCATATTAAATTAANAGCTACCGGTGGTAATG
GTTCAAAGACCCCAGGTCCAGGTGCTCAATCTGCCCTCAGAGCTTTAGCTCGTTCAGGTA
TGAGAATTGGTCGTATTGAAGATGTTACCCCAATTCCAACTGATTCAACCAGAAGAAAGA
GCGGTCATCGTGGTAGACGTTTATAAACATTTGGATCTCATCATTCAAGTGCTTTTTTAA
TAATAATACTACAACCTTTTTAATTTTAAAAAAAACCAAAAAAAAAACAATTTGTTTTAA
TACAACAACAACTCTAATAAAATAGGTA
Length of connected seq. 1158
Full length Seq ID -
Full length Seq. -
Length of full length seq. -