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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSI146
Library VS
(Link to library)
Clone ID VSI146
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12264-1
Original site URL
Representative seq. ID VSI146P
(Link to Original site)
Representative DNA sequence 
>VSI146 (VSI146Q) /CSM/VS/VSI1-B/VSI146Q.Seq.d/
CAAACAAATAAACAAATAGATAAATAATAAAATAATAATGCCACAAAAATTTTATTTATT
AATATTAACAGTTTTTTCAATATTATTAACAAAAATTAACTGTGAATTACAATTACCTTA
CTTTTATTCATTTGAAAGTACTGGAAATTTTCAAGTTATTAATGTTGAAACTAATGAAGC
CGTTTTAAATAGTACACTATCGATTGATTTACAGGTTTTATTAGTTTATGGGTCAACTAT
TTCCACAGTTTCAAATAAACCAATTTTTAATTTATTCGTTTCAGAAATAAATAGTAGAGT
TTTCTATACAATTGATTATGATTCAGATTCCAATCAATTTAGTAATAAAAGTGTACAATT
AACGTTACCAACTACTCTTGGTTTTGAATACGACCCTTCAACATTTTTCTACAACCCAGT
TACAAATTCATTCATTTTACCTTGTCACATTGATGGTTTAACTCAAAATGTTTTATCCTT
TATTGAATGGGATTTTAAAAATTCAAAAATAAATACTTATAAAAATGTAXXXXXXXXXXC
TTATAAAAATGTAGAAAGTTTTAAATATATGACACTTCCCAATATCAGCATATAATCCAT
ATACTGATGAATTATTTACATTTTTAACAGGTCCAAATAATGCACCCATATGTTCAAATT
AATATGAATAATTCATTTTCAGTTGATAATGTAAAGGTTATTAAATTCCCAAGTAAGAGT
GCAACATTACCAAATATTGGCAACATATTTATAAATACACAAGGTGGGTTAGTTGGTACT
GGCTATATTGAAGGAACATCGGAATTGTTTGTTTGTGAAATTGATTTCAAAACATTATCA
TGTTCAATCGAATATACAACTTTAATTTCATCATTTGATAATAATTTCTCTGTAATTTAT
CCATCATCAGATTTATCTTATTTAATTATATTGGTTGGAAATGGTTTCAATTGTGATACT
ATTGAAATTAATTATTTAAATTTATTTACATATAAAGTAGATTATACATTTAAAATTAAT
AATTTCTTTAAAAATCCTCAAGATAGTCCAGTACTTTTTTTCTAAAGTTAGTAAAAAAAA
TATTTTTTCCAAAA
sequence update 2001. 3.22
Translated Amino Acid sequence 
kqink*INNKIIMPQKFYLLILTVFSILLTKINCELQLPYFYSFESTGNFQVINVETNEA
VLNSTLSIDLQVLLVYGSTISTVSNKPIFNLFVSEINSRVFYTIDYDSDSNQFSNKSVQL
TLPTTLGFEYDPSTFFYNPVTNSFILPCHIDGLTQNVLSFIEWDFKNSKINTYKNV---

---YKNVESFKYMTLPNISI*siy**iiyifnrsk*cthmfkli*iihfqlim*rllnsq
vrvqhyqilatyl*ihkvg*lvlailkehrnclfvkliskhyhvqsniql*fhhliiisl
*fihhqiyli*lywlemvsivillklii*iylhik*iihlkliislkilkivqyffskvs
kknifsk
Translated Amino Acid sequence (All Frames) 
Frame A:
qtnkqidk**nnnatkilfininsffniinkn*l*ititlllfi*kywkfssy*c*n**s
rfk*ytid*ftgfislwvnyfhsfk*tnf*firfrnk**sflyn*l*frfqsi***kcti
nvtnyswf*irpfniflqpsykfihftlsh*wfnskcfily*mgf*kfknkyl*kc---

---likm*kvlni*hfpisaynpytdelftfltgpnnapicsn*ye*fifs**ckgy*ip
k*ecnitkywqhiykytrwvswywly*rnigivcl*n*fqniimfnriynfnfii***fl
cnlsiirfilfnyigwkwfql*yy*n*lfkfiyi*srlyi*n**fl*kssr*sstfflkl
vkkiffpk

Frame B:
kqink*INNKIIMPQKFYLLILTVFSILLTKINCELQLPYFYSFESTGNFQVINVETNEA
VLNSTLSIDLQVLLVYGSTISTVSNKPIFNLFVSEINSRVFYTIDYDSDSNQFSNKSVQL
TLPTTLGFEYDPSTFFYNPVTNSFILPCHIDGLTQNVLSFIEWDFKNSKINTYKNV---

---l*kcrkf*iydtsqyqhiihilmnylhf*qvqimhpyvqinmnnsfsvdnvkvikfp
sksatlpnignifintqgglvgtgyiegtselfvceidfktlscsieyttlissfdnnfs
viypssdlsyliilvgngfncdtieinylnlftykvdytfkinnffknpqdspvlff*s*
*kkyffq

Frame C:
nk*tnr*iik**chknfiy*y*qffqyy*qkltvnynyltfihlkvleifkllmlklmkp
f*ivhyrliyrfy*fmgqlfpqfqinqfliysfqk*ivefsiqlimiqipinlvikvyn*
ryqlllvlnttlqhfsttqlqihsfylvtlmv*lkmfypllngilkiqk*ilikm---

---YKNVESFKYMTLPNISI*siy**iiyifnrsk*cthmfkli*iihfqlim*rllnsq
vrvqhyqilatyl*ihkvg*lvlailkehrnclfvkliskhyhvqsniql*fhhliiisl
*fihhqiyli*lywlemvsivillklii*iylhik*iihlkliislkilkivqyffskvs
kknifsk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI146 (VSI146Q) /CSM/VS/VSI1-B/VSI146Q.Seq.d/                       1744   0.0
VFM448 (VFM448Q) /CSM/VF/VFM4-B/VFM448Q.Seq.d/                        950   0.0
SSF458 (SSF458Q) /CSM/SS/SSF4-C/SSF458Q.Seq.d/                        944   0.0
VSG149 (VSG149Q) /CSM/VS/VSG1-C/VSG149Q.Seq.d/                        936   0.0
VFH305 (VFH305Q) /CSM/VF/VFH3-A/VFH305Q.Seq.d/                        936   0.0
VFB355 (VFB355Q) /CSM/VF/VFB3-C/VFB355Q.Seq.d/                        936   0.0
VSH416 (VSH416Q) /CSM/VS/VSH4-A/VSH416Q.Seq.d/                        932   0.0
SSL234 (SSL234Q) /CSM/SS/SSL2-B/SSL234Q.Seq.d/                        252   1e-65
VSF777 (VSF777Q) /CSM/VS/VSF7-D/VSF777Q.Seq.d/                         46   0.001
SFE386 (SFE386Q) /CSM/SF/SFE3-D/SFE386Q.Seq.d/                         42   0.018
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ429488) Dictyostelium discoideum cDNA clone:ddv3n13, 3' e...   622   0.0    5
(AU266042) Dictyostelium discoideum vegetative cDNA clone:VS...   622   0.0    5
(BJ431689) Dictyostelium discoideum cDNA clone:ddv15j01, 3' ...   622   0.0    4
(BJ411266) Dictyostelium discoideum cDNA clone:ddv3n13, 5' e...   882   0.0    2
(BJ434932) Dictyostelium discoideum cDNA clone:ddv25p12, 3' ...   622   0.0    5
(BJ416258) Dictyostelium discoideum cDNA clone:ddv25p12, 5' ...   882   0.0    2
(AU268223) Dictyostelium discoideum vegetative cDNA clone:VS...   882   0.0    1
(AU266041) Dictyostelium discoideum vegetative cDNA clone:VS...   882   0.0    1
(AU267413) Dictyostelium discoideum vegetative cDNA clone:VS...   864   0.0    1
(AU268224) Dictyostelium discoideum vegetative cDNA clone:VS...   833   0.0    3
dna update 2009. 7. 3
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP001130_820(CP001130|pid:none) Hydrogenobaculum sp. Y04AAS1, co...    38   0.67
AF020283_1(AF020283|pid:none) Dictyostelium discoideum DG2044 ge...    35   3.3
BA000045_213(BA000045|pid:none) Gloeobacter violaceus PCC 7421 D...    35   5.7
BX571860_199(BX571860|pid:none) Photorhabdus luminescens subsp. ...    35   5.7
AC117075_65(AC117075|pid:none) Dictyostelium discoideum chromoso...    35   5.7
AL844509_252(AL844509|pid:none) Plasmodium falciparum 3D7 chromo...    34   7.4
(Q859W7) RecName: Full=Protein ycf2;  &AB086179_21(AB086179|pid:...    34   9.7
protein update 2009. 3.24
PSORT 

 psg: 0.96  gvh: 0.64  alm: 0.31  top: 0.27  tms: 0.07  mit: 0.32  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: cytoplasmic
	 20.0 %: nuclear
	 20.0 %: endoplasmic reticulum
	 12.0 %: Golgi
	 12.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for VSI146 is cyt
5' end seq. ID VSI146F
5' end seq. 
>VSI146F.Seq
CAAACAAATAAACAAATAGATAAATAATAAAATAATAATGCCACAAAAATTTTATTTATT
AATATTAACAGTTTTTTCAATATTATTAACAAAAATTAACTGTGAATTACAATTACCTTA
CTTTTATTCATTTGAAAGTACTGGAAATTTTCAAGTTATTAATGTTGAAACTAATGAAGC
CGTTTTAAATAGTACACTATCGATTGATTTACAGGTTTTATTAGTTTATGGGTCAACTAT
TTCCACAGTTTCAAATAAACCAATTTTTAATTTATTCGTTTCAGAAATAAATAGTAGAGT
TTTCTATACAATTGATTATGATTCAGATTCCAATCAATTTAGTAATAAAAGTGTACAATT
AACGTTACCAACTACTCTTGGTTTTGAATACGACCCTTCAACATTTTTCTACAACCCAGT
TACAAATTCATTCATTTTACCTTGTCACATTGATGGTTTAACTCAAAATGTTTTATCCTT
TATTGAATGGGATTTTAAAAATTCAAAAATAAATACTTATAAAAATGTA----------
Length of 5' end seq. 529
3' end seq. ID VSI146Z
3' end seq. 
>VSI146Z.Seq
----------CTTATAAAAATGTAGAAAGTTTTAAATATATGACACTTCCCAATATCAGC
ATATAATCCATATACTGATGAATTATTTACATTTTTAACAGGTCCAAATAATGCACCCAT
ATGTTCAAATTAATATGAATAATTCATTTTCAGTTGATAATGTAAAGGTTATTAAATTCC
CAAGTAAGAGTGCAACATTACCAAATATTGGCAACATATTTATAAATACACAAGGTGGGT
TAGTTGGTACTGGCTATATTGAAGGAACATCGGAATTGTTTGTTTGTGAAATTGATTTCA
AAACATTATCATGTTCAATCGAATATACAACTTTAATTTCATCATTTGATAATAATTTCT
CTGTAATTTATCCATCATCAGATTTATCTTATTTAATTATATTGGTTGGAAATGGTTTCA
ATTGTGATACTATTGAAATTAATTATTTAAATTTATTTACATATAAAGTAGATTATACAT
TTAAAATTAATAATTTCTTTAAAAATCCTCAAGATAGTCCAGTACTTTTTTTCTAAAGTT
AGTAAAAAAAATATTTTTTCCAAAA
Length of 3' end seq. 555
Connected seq. ID VSI146P
Connected seq. 
>VSI146P.Seq
CAAACAAATAAACAAATAGATAAATAATAAAATAATAATGCCACAAAAATTTTATTTATT
AATATTAACAGTTTTTTCAATATTATTAACAAAAATTAACTGTGAATTACAATTACCTTA
CTTTTATTCATTTGAAAGTACTGGAAATTTTCAAGTTATTAATGTTGAAACTAATGAAGC
CGTTTTAAATAGTACACTATCGATTGATTTACAGGTTTTATTAGTTTATGGGTCAACTAT
TTCCACAGTTTCAAATAAACCAATTTTTAATTTATTCGTTTCAGAAATAAATAGTAGAGT
TTTCTATACAATTGATTATGATTCAGATTCCAATCAATTTAGTAATAAAAGTGTACAATT
AACGTTACCAACTACTCTTGGTTTTGAATACGACCCTTCAACATTTTTCTACAACCCAGT
TACAAATTCATTCATTTTACCTTGTCACATTGATGGTTTAACTCAAAATGTTTTATCCTT
TATTGAATGGGATTTTAAAAATTCAAAAATAAATACTTATAAAAATGTA----------C
TTATAAAAATGTAGAAAGTTTTAAATATATGACACTTCCCAATATCAGCATATAATCCAT
ATACTGATGAATTATTTACATTTTTAACAGGTCCAAATAATGCACCCATATGTTCAAATT
AATATGAATAATTCATTTTCAGTTGATAATGTAAAGGTTATTAAATTCCCAAGTAAGAGT
GCAACATTACCAAATATTGGCAACATATTTATAAATACACAAGGTGGGTTAGTTGGTACT
GGCTATATTGAAGGAACATCGGAATTGTTTGTTTGTGAAATTGATTTCAAAACATTATCA
TGTTCAATCGAATATACAACTTTAATTTCATCATTTGATAATAATTTCTCTGTAATTTAT
CCATCATCAGATTTATCTTATTTAATTATATTGGTTGGAAATGGTTTCAATTGTGATACT
ATTGAAATTAATTATTTAAATTTATTTACATATAAAGTAGATTATACATTTAAAATTAAT
AATTTCTTTAAAAATCCTCAAGATAGTCCAGTACTTTTTTTCTAAAGTTAGTAAAAAAAA
TATTTTTTCCAAAA
Length of connected seq. 1084
Full length Seq ID -
Full length Seq. -
Length of full length seq. -