>VSI144 (VSI144Q) /CSM/VS/VSI1-B/VSI144Q.Seq.d/
TTTTCATCTGCACCTACAGTGAATCGTGGTTTGGGACTCGATTCAATGGGATCGGGTATC
ATTTTCACCATTCTTGGTTTCTTTGGTTTCTTATGGCAAATTTTATTGTTCAAAAAGGTT
TCAGGAAGATTCGGTCTAAAGAGACAATATACAGTTGGTATGATTTTATTATCGATTGGT
ATGGCATTGTTCCCATTATCTTATGTCGCTTGGAATATTCAAGGTTATCCATTGGTTTGG
GTTACCATTGGTATCGTGGTGCCAATCATTTCCGTTGGTTTCATGAGTGGACTTCCAATT
GTACAAGGTATGGTTGCAAATTGTTCAAATCCTGAAATTCAAGGTTTAACTCAAGGTTCA
ACCCAATCCATCAATTCAATGCTTCGTTCATTTGGTCCAGCAATCTCTGGTGCCATTTTC
AGTGCTTCAACCGCTCATAGTCAACCATGGGTACTCTTTATTGTTCTCTCCATAATTTAT
GTTTGTGTGGCTATCGTTAGTCTTTATTTACCTGATACAGTTGATATCAATCATAAAGCT
TTAAAACAAAAGAGAGAATTAAGAAAAAATAATTCAAATTAATCTTTTTTTTTTTTTTTT
NCCTTTTNAAAAAAAAAXXXXXXXXXX

FSSAPTVNRGLGLDSMGSGIIFTILGFFGFLWQILLFKKVSGRFGLKRQYTVGMILLSIG
MALFPLSYVAWNIQGYPLVWVTIGIVVPIISVGFMSGLPIVQGMVANCSNPEIQGLTQGS
TQSINSMLRSFGPAISGAIFSASTAHSQPWVLFIVLSIIYVCVAIVSLYLPDTVDINHKA
LKQKRELRKNNSN*sfffffxfxkk---

Frame A:
FSSAPTVNRGLGLDSMGSGIIFTILGFFGFLWQILLFKKVSGRFGLKRQYTVGMILLSIG
MALFPLSYVAWNIQGYPLVWVTIGIVVPIISVGFMSGLPIVQGMVANCSNPEIQGLTQGS
TQSINSMLRSFGPAISGAIFSASTAHSQPWVLFIVLSIIYVCVAIVSLYLPDTVDINHKA
LKQKRELRKNNSN*sfffffxfxkk---


Frame B:
fhlhlq*ivvwdsiqwdrvsfspflvslvsygkfycskrfqedsv*rdniqlv*fyyrlv
whcshylmslgifkvihwfglplvswcqsfplvs*vdfqlykvwlqivqilkfkv*lkvq
pnpsiqcfvhlvqqslvpfsvlqplivnhgysllfsp*fmfvwlslvfiyliqlisiikl
*nkren*ekiiqinlffffxpfxkk---


Frame C:
fictyseswfgtrfngigyhfhhswflwflmanfivqkgfrkirsketiyswydfiidwy
givpiilcrleysrlsiglgyhwyrganhfrwfhewtsnctrygcklfks*nsrfnsrfn
pihqfnasfiwssnlwchfqcfnrs*stmgtlycslhnlclcgyr*slft*ys*yqs*sf
ktkerikkk*fklifffffxlxkk---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI144 (VSI144Q) /CSM/VS/VSI1-B/VSI144Q.Seq.d/                       1158   0.0
VSE117 (VSE117Q) /CSM/VS/VSE1-A/VSE117Q.Seq.d/                         38   0.16
FC-BR08 (FC-BR08Q) /CSM/FC/FC-BR/FC-BR08Q.Seq.d/                       38   0.16
SFI564 (SFI564Q) /CSM/SF/SFI5-C/SFI564Q.Seq.d/                         36   0.62
SFF536 (SFF536Q) /CSM/SF/SFF5-B/SFF536Q.Seq.d/                         36   0.62
CFG401 (CFG401Q) /CSM/CF/CFG4-A/CFG401Q.Seq.d/                         36   0.62
VSB471 (VSB471Q) /CSM/VS/VSB4-C/VSB471Q.Seq.d/                         34   2.4
SSJ724 (SSJ724Q) /CSM/SS/SSJ7-A/SSJ724Q.Seq.d/                         34   2.4
SSJ187 (SSJ187Q) /CSM/SS/SSJ1-D/SSJ187Q.Seq.d/                         34   2.4
SLB253 (SLB253Q) /CSM/SL/SLB2-C/SLB253Q.Seq.d/                         34   2.4

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU268220) Dictyostelium discoideum vegetative cDNA clone:VS...  1158   0.0    1
(M81834) Homo sapiens alpha-2 collagen type VI (COL6A2) gene...    50   0.10   1
(AC210996) Homo sapiens FOSMID clone ABC9-44046100B9 from ch...    50   0.10   1
(AC090614) Homo sapiens chromosome 17, clone RP11-577I13, co...    50   0.10   1
(AC005341) Homo sapiens chromosome , clone RP11-146P2, compl...    50   0.10   1
(AC069003) Homo sapiens chromosome 17 clone RP11-213B3, WORK...    50   0.10   1
(AC026873) Homo sapiens chromosome 17 clone RP11-577I13, WOR...    50   0.10   1
(AC123504) Rattus norvegicus clone CH230-48L14, *** SEQUENCI...    38   0.25   3
(AC133842) Rattus norvegicus clone CH230-185E3, *** SEQUENCI...    42   0.33   5
(AC119716) Rattus norvegicus clone CH230-274I16, *** SEQUENC...    42   0.34   5

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000909_1543(CP000909|pid:none) Chloroflexus aurantiacus J-10-f...    51   2e-05
AB043474_7(AB043474|pid:none) Shuttle vector pNIT6010 DNA, compl...    49   9e-05
AJ628353_13(AJ628353|pid:none) Salmonella enterica plasmid 6/9 m...    49   9e-05
AY903253_1(AY903253|pid:none) Laribacter hongkongensis tetracycl...    49   9e-05
L29404_7(L29404|pid:none) E. coli mercuric reductase (merA) gene...    49   9e-05
U75324_2(U75324|pid:none) Cloning vector pJB3Tc20, complete sequ...    49   9e-05
AB114188_15(AB114188|pid:none) Vibrio cholerae DNA, integrating ...    49   9e-05
(P02982) RecName: Full=Tetracycline resistance protein, class A;...    49   9e-05
AF497970_2(AF497970|pid:none) Shigella sonnei plasmid pKKTET7, c...    49   9e-05
AB027254_3(AB027254|pid:none) Cloning vector pSB4 tetR, tetA, hy...    49   9e-05

 psg: 0.79  gvh: 0.62  alm: 0.14  top: 0.80  tms: 0.27  mit: 0.15  mip: 0.21
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 1.00  m_ : 0.00 

	 32.0 %: endoplasmic reticulum
	 20.0 %: plasma membrane
	 12.0 %: nuclear
	 12.0 %: mitochondrial
	 12.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: cytoplasmic

>> prediction for VSI144 is end

>VSI144F.Seq
TTTTCATCTGCACCTACAGTGAATCGTGGTTTGGGACTCGATTCAATGGGATCGGGTATC
ATTTTCACCATTCTTGGTTTCTTTGGTTTCTTATGGCAAATTTTATTGTTCAAAAAGGTT
TCAGGAAGATTCGGTCTAAAGAGACAATATACAGTTGGTATGATTTTATTATCGATTGGT
ATGGCATTGTTCCCATTATCTTATGTCGCTTGGAATATTCAAGGTTATCCATTGGTTTGG
GTTACCATTGGTATCGTGGTGCCAATCATTTCCGTTGGTTTCATGAGTGGACTTCCAATT
GTACAAGGTATGGTTGCAAATTGTTCAAATCCTGAAATTCAAGGTTTAACTCAAGGTTCA
ACCCAATCCATCAATTCAATGCTTCGTTCATTTGGTCCAGCAATCTCTGGTGCCATTTTC
AGTGCTTCAACCGCTCATAGTCAACCATGGGTACTCTTTATTGTTCTCTCCATAATTTAT
GTTTGTGTGGCTATCGTTAGTCTTTATTTACCTGATACAGTTGATATCAATCATAAAGCT
TTAAAACAAAAGAGAGAATTAAGAAAAAATAATTCAAATTAATCTTTTTTTTTTTTTTTT
NCCTTTTNAAAAAAAAA----------