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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSI127
Library VS
(Link to library)
Clone ID VSI127
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U04565-1
Original site URL
Representative seq. ID VSI127P
(Link to Original site)
Representative DNA sequence 
>VSI127 (VSI127Q) /CSM/VS/VSI1-B/VSI127Q.Seq.d/
CTTAACAAATAGTAGATCATTTAGTACCAATACCGGAAGTGGTAGCAAAATTGGTAATAT
TATTGGTAAAATTGGAATTGGTAAATTAAATCATGTTGCAATTGCCACACCAAATTTACA
AGAGTCAATGGATTTATACAAAAATGTTTTTGGTGCTGATGTTAGTGAACCAGTGAATCA
AGTTGAACATGGTGTAACAACAGTATTTGTTGGATTAGAAAATACAAAAATTGAATTACT
CCATCCATTTGGAGATAAATCACCAATTGAAAATTTTTTAAAAAAGAATCCATCAGGTGG
TATTCATCATATTTGTTTAGAAGTTGATGATATTCATCATGCAGTCAAAACTTTATTAGC
AGAAAATGTTAGAATTATTGATCCAACACCAAAGATTGGTGCTCATGGTAAACCAGTTGT
TTTCTTACATCCAAAATCAATGAATGGTGTTTTAGTAGAATTAGAAGAAAAATAAAAATA
ATATATAGAATTTTTTTTAAAAAAAAAAXXXXXXXXXXCTTAACAAATAGTAGATCATTT
AGTACCAATACCGGAAGTGGTAGCAAAATTGGTAATATTATTGGTAAAATTGGAATTGGT
AAATTAAATCATGTTGCAATTGCCACACCAAATTTACAAGAGTCAATGGATTTATACAAA
AATGTTTTTGGTGCTGATGTTAGTGAACCAGTGAATCAAGTTGAACATGGTGTAACAACA
GTATTTGTTGGATTAGAAAATACAAAAATTGAATTACTCCATCCATTTGGAGATAAATCA
CCAATTGAAAATTTTTTAAAAAAGAATCCATCAGGTGGTATTCATCATATTTGTTTAGAA
GTTGATGATATTCATCATGCAGTCAAAACTTTATTAGCAGAAAATGTTAGAATTATTGAT
CCAACACCAAAGATTGGTGCTCATGGTAAACCAGTTGTTTTCTTACATCCAAAATCAATG
AATGGTGTTTTAGTAGAATTAGAAGAAAAATAAAAATAAT
sequence update 2001. 3.22
Translated Amino Acid sequence 
lnk**ii*yqyrkw*qnw*yyw*nwnw*iksccnchtkftrvngfiqkcfwc*c**tses
s*twcnnsicwirkykn*itpsiwr*itn*kffkkesirwyssylfrs**ysscsqnfis
rkc*ny*sntkdwcsw*TSCFLTSKINEWCFSRIRRKIKIIYRIFFKKK---

---LTNSRSFSTNTGSGSKIGNIIGKIGIGKLNHVAIATPNLQESMDLYKNVFGADVSEP
VNQVEHGVTTVFVGLENTKIELLHPFGDKSPIENFLKKNPSGGIHHICLEVDDIHHAVKT
LLAENVRIIDPTPKIGAHGKPVVFLHPKSMNGVLVELEEK*k*
Translated Amino Acid sequence (All Frames) 
Frame A:
lnk**ii*yqyrkw*qnw*yyw*nwnw*iksccnchtkftrvngfiqkcfwc*c**tses
s*twcnnsicwirkykn*itpsiwr*itn*kffkkesirwyssylfrs**ysscsqnfis
rkc*ny*sntkdwcsw*TSCFLTSKINEWCFSRIRRKIKIIYRIFFKKK---

---lnk**ii*yqyrkw*qnw*yyw*nwnw*iksccnchtkftrvngfiqkcfwc*c**t
sess*twcnnsicwirkykn*itpsiwr*itn*kffkkesirwyssylfrs**ysscsqn
fisrkc*ny*sntkdwcsw*tscfltskinewcfsrirrkiki

Frame B:
ltnsrsfstntgsgskigniigkigigklnhvaiatpnlqesmdlyknvfgadvsepvnq
vehgvttvfvglentkiellhpfgdkspienflkknpsggihhiclevddihhavktlla
envriidptpkigahgkpvvflhpksmngvlveleek*k*yiefflkkk---

---LTNSRSFSTNTGSGSKIGNIIGKIGIGKLNHVAIATPNLQESMDLYKNVFGADVSEP
VNQVEHGVTTVFVGLENTKIELLHPFGDKSPIENFLKKNPSGGIHHICLEVDDIHHAVKT
LLAENVRIIDPTPKIGAHGKPVVFLHPKSMNGVLVELEEK*k*

Frame C:
*qivdhlvpipevvaklvillvklelvn*imlqlphqiyksqwiytkmflvlmlvnq*ik
lnmv*qqylld*kiqklnysihleinhqlkif*krihqvvfiifv*klmifimqskly*q
kmlelliqhqrlvlmvnqlfsyiqnq*mvf**n*kknknni*nff*kk---

---*qivdhlvpipevvaklvillvklelvn*imlqlphqiyksqwiytkmflvlmlvnq
*iklnmv*qqylld*kiqklnysihleinhqlkif*krihqvvfiifv*klmifimqskl
y*qkmlelliqhqrlvlmvnqlfsyiqnq*mvf**n*kknknn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI127 (VSI127Q) /CSM/VS/VSI1-B/VSI127Q.Seq.d/                        987   0.0
VSG686 (VSG686Q) /CSM/VS/VSG6-D/VSG686Q.Seq.d/                        973   0.0
VSG436 (VSG436Q) /CSM/VS/VSG4-B/VSG436Q.Seq.d/                        914   0.0
SSB502 (SSB502Q) /CSM/SS/SSB5-A/SSB502Q.Seq.d/                        400   e-110
SSJ266 (SSJ266Q) /CSM/SS/SSJ2-C/SSJ266Q.Seq.d/                         40   0.064
VSD428 (VSD428Q) /CSM/VS/VSD4-B/VSD428Q.Seq.d/                         38   0.25
VFN342 (VFN342Q) /CSM/VF/VFN3-B/VFN342Q.Seq.d/                         38   0.25
VFM422 (VFM422Q) /CSM/VF/VFM4-A/VFM422Q.Seq.d/                         38   0.25
SSC560 (SSC560Q) /CSM/SS/SSC5-C/SSC560Q.Seq.d/                         38   0.25
SSA739 (SSA739Q) /CSM/SS/SSA7-B/SSA739Q.Seq.d/                         38   0.25
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU268193) Dictyostelium discoideum vegetative cDNA clone:VS...   971   0.0    1
(AU266725) Dictyostelium discoideum vegetative cDNA clone:VS...   898   0.0    2
(AU268194) Dictyostelium discoideum vegetative cDNA clone:VS...   955   0.0    1
(AU266478) Dictyostelium discoideum vegetative cDNA clone:VS...   866   0.0    2
(AC116305) Dictyostelium discoideum chromosome 2 map 1005175...   626   0.0    6
(AU266477) Dictyostelium discoideum vegetative cDNA clone:VS...   880   0.0    1
(AU071440) Dictyostelium discoideum slug cDNA, clone SSB502.      208   4e-96  2
(AU037123) Dictyostelium discoideum slug cDNA, clone SSB502.      329   1e-85  1
(ES570016) TPAF-aaa70g06.g1 T.spiralis_EST Trichinella spira...    58   2e-12  3
(EX501485) TPAF-aac41f02.g1 T.spiralis_EST Trichinella spira...    58   2e-12  3
dna update 2009. 7. 3
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q553V2) RecName: Full=Methylmalonyl-CoA epimerase, mitochondria...   279   9e-74
AC116305_9(AC116305|pid:none) Dictyostelium discoideum chromosom...   264   4e-69
BC095773_1(BC095773|pid:none) Danio rerio methylmalonyl CoA epim...   168   3e-40
EF064249_1(EF064249|pid:none) Blastocladiella emersonii methylma...   166   8e-40
AY594301_1(AY594301|pid:none) Caenorhabditis elegans mitochondri...   166   1e-39
AP007255_2575(AP007255|pid:none) Magnetospirillum magneticum AMB...   162   2e-38
CP000377_637(CP000377|pid:none) Silicibacter sp. TM1040, complet...   160   8e-38
(Q96PE7) RecName: Full=Methylmalonyl-CoA epimerase, mitochondria...   159   1e-37
BC020825_1(BC020825|pid:none) Homo sapiens methylmalonyl CoA epi...   158   2e-37
DQ629149_1(DQ629149|pid:none) Sus scrofa MCEE (MCEE) mRNA, compl...   158   2e-37
protein update 2009. 3.24
PSORT 

 psg: 0.49  gvh: 0.40  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.42  mip: 0.07
 nuc: 0.06  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 36.0 %: mitochondrial
	  8.0 %: cytoplasmic
	  4.0 %: vesicles of secretory system

>> prediction for VSI127 is nuc
5' end seq. ID VSI127F
5' end seq. 
>VSI127F.Seq
CTTAACAAATAGTAGATCATTTAGTACCAATACCGGAAGTGGTAGCAAAATTGGTAATAT
TATTGGTAAAATTGGAATTGGTAAATTAAATCATGTTGCAATTGCCACACCAAATTTACA
AGAGTCAATGGATTTATACAAAAATGTTTTTGGTGCTGATGTTAGTGAACCAGTGAATCA
AGTTGAACATGGTGTAACAACAGTATTTGTTGGATTAGAAAATACAAAAATTGAATTACT
CCATCCATTTGGAGATAAATCACCAATTGAAAATTTTTTAAAAAAGAATCCATCAGGTGG
TATTCATCATATTTGTTTAGAAGTTGATGATATTCATCATGCAGTCAAAACTTTATTAGC
AGAAAATGTTAGAATTATTGATCCAACACCAAAGATTGGTGCTCATGGTAAACCAGTTGT
TTTCTTACATCCAAAATCAATGAATGGTGTTTTAGTAGAATTAGAAGAAAAATAAAAATA
ATATATAGAATTTTTTTTAAAAAAAAAA----------
Length of 5' end seq. 508
3' end seq. ID VSI127Z
3' end seq. 
>VSI127Z.Seq
----------CTTAACAAATAGTAGATCATTTAGTACCAATACCGGAAGTGGTAGCAAAA
TTGGTAATATTATTGGTAAAATTGGAATTGGTAAATTAAATCATGTTGCAATTGCCACAC
CAAATTTACAAGAGTCAATGGATTTATACAAAAATGTTTTTGGTGCTGATGTTAGTGAAC
CAGTGAATCAAGTTGAACATGGTGTAACAACAGTATTTGTTGGATTAGAAAATACAAAAA
TTGAATTACTCCATCCATTTGGAGATAAATCACCAATTGAAAATTTTTTAAAAAAGAATC
CATCAGGTGGTATTCATCATATTTGTTTAGAAGTTGATGATATTCATCATGCAGTCAAAA
CTTTATTAGCAGAAAATGTTAGAATTATTGATCCAACACCAAAGATTGGTGCTCATGGTA
AACCAGTTGTTTTCTTACATCCAAAATCAATGAATGGTGTTTTAGTAGAATTAGAAGAAA
AATAAAAATAAT
Length of 3' end seq. 482
Connected seq. ID VSI127P
Connected seq. 
>VSI127P.Seq
CTTAACAAATAGTAGATCATTTAGTACCAATACCGGAAGTGGTAGCAAAATTGGTAATAT
TATTGGTAAAATTGGAATTGGTAAATTAAATCATGTTGCAATTGCCACACCAAATTTACA
AGAGTCAATGGATTTATACAAAAATGTTTTTGGTGCTGATGTTAGTGAACCAGTGAATCA
AGTTGAACATGGTGTAACAACAGTATTTGTTGGATTAGAAAATACAAAAATTGAATTACT
CCATCCATTTGGAGATAAATCACCAATTGAAAATTTTTTAAAAAAGAATCCATCAGGTGG
TATTCATCATATTTGTTTAGAAGTTGATGATATTCATCATGCAGTCAAAACTTTATTAGC
AGAAAATGTTAGAATTATTGATCCAACACCAAAGATTGGTGCTCATGGTAAACCAGTTGT
TTTCTTACATCCAAAATCAATGAATGGTGTTTTAGTAGAATTAGAAGAAAAATAAAAATA
ATATATAGAATTTTTTTTAAAAAAAAAA----------CTTAACAAATAGTAGATCATTT
AGTACCAATACCGGAAGTGGTAGCAAAATTGGTAATATTATTGGTAAAATTGGAATTGGT
AAATTAAATCATGTTGCAATTGCCACACCAAATTTACAAGAGTCAATGGATTTATACAAA
AATGTTTTTGGTGCTGATGTTAGTGAACCAGTGAATCAAGTTGAACATGGTGTAACAACA
GTATTTGTTGGATTAGAAAATACAAAAATTGAATTACTCCATCCATTTGGAGATAAATCA
CCAATTGAAAATTTTTTAAAAAAGAATCCATCAGGTGGTATTCATCATATTTGTTTAGAA
GTTGATGATATTCATCATGCAGTCAAAACTTTATTAGCAGAAAATGTTAGAATTATTGAT
CCAACACCAAAGATTGGTGCTCATGGTAAACCAGTTGTTTTCTTACATCCAAAATCAATG
AATGGTGTTTTAGTAGAATTAGAAGAAAAATAAAAATAAT
Length of connected seq. 990
Full length Seq ID -
Full length Seq. -
Length of full length seq. -