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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSI112
Library VS
(Link to library)
Clone ID VSI112
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14933-1
Original site URL
Representative seq. ID VSI112E
(Link to Original site)
Representative DNA sequence 
>VSI112 (VSI112Q) /CSM/VS/VSI1-A/VSI112Q.Seq.d/
TTCCCACCATGTGGTGAATTGGCCTCACCAATCATGCATTTTGATAATGTTACTTTTTCA
TACAGCGGTAAAGAAGCAGATGTTTTATATAGAAATTTAGATTTAGCTATCGATTTAGAC
TCTAGAATTGCACTTGTTGGTCCAAATGGTGCTGGTAAAAGTACTTTATTGAAGCTTATG
GTCGGTCAAATCTCACCAACTCAAGGTTTCATTAAAAAACATAGTCATCTTAAAATGGCT
CGTTATCATCAACATGCTCATGAAGTTTTAGATCTCACCGCTACTCCATTGGATTTTGTT
CGTTCTAAATTTGCTCATATGAATAAAGATACCGAAGAATGGCGTCGTGAAATTGGTAGA
TTTGGTGTTACTGGTAAAGCTCAAACTGAAGCTATTGGTTGTATGTCTGATGGTATTAAA
TCTCGTTTAATTTTTCTGTCTTATGGCTCTCGAAAATCCACATCTTTTATTACTTGATGA
ACCAACAAATCATTTAGATATGGAATGTATTGATAGTTTAGCTTTGGCCATCAATTCTTT
CCCCGGAGGCATGATATTAGTATCTCACGATTTTAGATTAATCTCTCAAGTTGCAAAAGA
AATTTGGGTTTGTGATAATAAAACTATTACTAAATGGGCAGGTGATATCACAAGCTATAA
GAATCACTTAAAGGCTCAAATGAGAGATCTCACAAAGCAAGGCGCATTAGCATCATTAAA
AAAATAATAGATAATAATATAA
sequence update 2001. 3.23
Translated Amino Acid sequence 
FPPCGELASPIMHFDNVTFSYSGKEADVLYRNLDLAIDLDSRIALVGPNGAGKSTLLKLM
VGQISPTQGFIKKHSHLKMARYHQHAHEVLDLTATPLDFVRSKFAHMNKDTEEWRREIGR
FGVTGKAQTEAIGCMSDGIKSRLIFLSYGSRKSTSFIT**tnksfrygmy**fsfghqff
prrhdisisrf*inlssckrnlgl***nyy*mgr*yhkl*eslkgsnershkarrisiik
kiidnni
Translated Amino Acid sequence (All Frames) 
Frame A:
FPPCGELASPIMHFDNVTFSYSGKEADVLYRNLDLAIDLDSRIALVGPNGAGKSTLLKLM
VGQISPTQGFIKKHSHLKMARYHQHAHEVLDLTATPLDFVRSKFAHMNKDTEEWRREIGR
FGVTGKAQTEAIGCMSDGIKSRLIFLSYGSRKSTSFIT**tnksfrygmy**fsfghqff
prrhdisisrf*inlssckrnlgl***nyy*mgr*yhkl*eslkgsnershkarrisiik
kiidnni


Frame B:
shhvvnwphqscilimllfhtavkkqmfyiei*i*lsi*tlelhllvqmvlvkvly*slw
svkshqlkvslknivilkwlviinmlmkf*ispllhwilfvlnlli*ikipkngvvklvd
lvllvklklkllvvclmvlnlv*ffclmalenphlllldeptnhldmecidslalainsf
pggmilvshdfrlisqvakeiwvcdnktitkwagditsyknhlkaqmrdltkqgalaslk
k**iii*


Frame C:
ptmw*igltnhaf**cyffiqr*rsrcfi*kfrfsyrfrl*nctcwskwcw*kyfieayg
rsnltnsrfh*kt*ss*ngslsstcs*sfrshrysigfcsf*icsye*ryrrmas*nw*i
wcyw*ssn*sywlyv*wy*isfnfsvlwlskihifyylmnqqii*iwnvliv*lwpsils
pea*y*yltild*slklqkkfgfviiklllngqvisqairit*rlk*eisqskah*hh*k
nnr**y
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI112 (VSI112Q) /CSM/VS/VSI1-A/VSI112Q.Seq.d/                       1429   0.0
AFK870 (AFK870Q) /CSM/AF/AFK8-C/AFK870Q.Seq.d/                       1405   0.0
CHE373 (CHE373Q) /CSM/CH/CHE3-D/CHE373Q.Seq.d/                       1398   0.0
AFA622 (AFA622Q) /CSM/AF/AFA6-A/AFA622Q.Seq.d/                       1398   0.0
CFI726 (CFI726Q) /CSM/CF/CFI7-B/CFI726Q.Seq.d/                       1376   0.0
SFG269 (SFG269Q) /CSM/SF/SFG2-C/SFG269Q.Seq.d/                       1372   0.0
SFK663 (SFK663Q) /CSM/SF/SFK6-C/SFK663Q.Seq.d/                       1364   0.0
CFH411 (CFH411Q) /CSM/CF/CFH4-A/CFH411Q.Seq.d/                       1364   0.0
CHR268 (CHR268Q) /CSM/CH/CHR2-C/CHR268Q.Seq.d/                       1360   0.0
VSG243 (VSG243Q) /CSM/VS/VSG2-B/VSG243Q.Seq.d/                       1356   0.0
own update 2003. 7. 8
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AF479254) Dictyostelium discoideum non-transporter ABC prot...   852   0.0    4
(BJ400522) Dictyostelium discoideum cDNA clone:dds13i18, 3' ...   852   0.0    2
(BJ376953) Dictyostelium discoideum cDNA clone:ddc30b19, 3' ...   852   0.0    2
(BJ375320) Dictyostelium discoideum cDNA clone:ddc18d07, 3' ...   852   0.0    3
(BJ343304) Dictyostelium discoideum cDNA clone:dda22l18, 3' ...   852   0.0    3
(AU266185) Dictyostelium discoideum vegetative cDNA clone:VS...   813   0.0    2
(BJ374848) Dictyostelium discoideum cDNA clone:ddc16c17, 3' ...   813   0.0    2
(BJ372941) Dictyostelium discoideum cDNA clone:ddc15f04, 3' ...   811   0.0    3
(BJ385921) Dictyostelium discoideum cDNA clone:ddc56g18, 3' ...   807   0.0    3
(BJ401260) Dictyostelium discoideum cDNA clone:dds22m16, 3' ...   797   0.0    4
dna update 2009. 7. 3
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF479254_1(AF479254|pid:none) Dictyostelium discoideum non-trans...   295   e-132
AJ719522_1(AJ719522|pid:none) Gallus gallus mRNA for hypothetica...   157   3e-69
AC021097_2(AC021097|pid:none) Homo sapiens PAC clone RP4-548D19 ...   156   2e-68
AK137233_1(AK137233|pid:none) Mus musculus adult male urinary bl...   156   2e-68
BC129119_1(BC129119|pid:none) Rattus norvegicus ATP-binding cass...   156   2e-68
AF261091_1(AF261091|pid:none) Homo sapiens iron inhibited ABC tr...   156   2e-68
(Q9UG63) RecName: Full=ATP-binding cassette sub-family F member ...   156   2e-68
AJ005016_1(AJ005016|pid:none) Homo sapiens mRNA for putative ABC...   156   2e-68
AE014298_2288(AE014298|pid:none) Drosophila melanogaster chromos...   155   1e-67
AF213382_1(AF213382|pid:none) Mus musculus ATP-binding cassette ...   156   1e-67
protein update 2009. 3.24
PSORT 

 psg: 0.75  gvh: 0.34  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VSI112 is cyt
5' end seq. ID VSI112F
5' end seq. 
>VSI112F.Seq
TTCCCACCATGTGGTGAATTGGCCTCACCAATCATGCATTTTGATAATGTTACTTTTTCA
TACAGCGGTAAAGAAGCAGATGTTTTATATAGAAATTTAGATTTAGCTATCGATTTAGAC
TCTAGAATTGCACTTGTTGGTCCAAATGGTGCTGGTAAAAGTACTTTATTGAAGCTTATG
GTCGGTCAAATCTCACCAACTCAAGGTTTCATTAAAAAACATAGTCATCTTAAAATGGCT
CGTTATCATCAACATGCTCATGAAGTTTTAGATCTCACCGCTACTCCATTGGATTTTGTT
CGTTCTAAATTTGCTCATATGAATAAAGATACCGAAGAATGGCGTCGTGAAATTGGTAGA
TTTGGTGTTACTGGTAAAGCTCAAACTGAAGCTATTGGTTGTATGTCTGATGGTATTAAA
TCTCGTTTAATTTTTCTGTCTTATGGCTCTCGAAAATCCACATCTTTTATTACTTGATGA
ACCAACAAATCATTTAGATATGGA----------
Length of 5' end seq. 504
3' end seq. ID VSI112Z
3' end seq. 
>VSI112Z.Seq
----------TATTACTTGATGAACCAACAAATCATTTAGATATGGAATGTATTGATAGT
TTAGCTTTGGCCATCAATTCTTTCCCCGGAGGCATGATATTAGTATCTCACGATTTTAGA
TTAATCTCTCAAGTTGCAAAAGAAATTTGGGTTTGTGATAATAAAACTATTACTAAATGG
GCAGGTGATATCACAAGCTATAAGAATCACTTAAAGGCTCAAATGAGAGATCTCACAAAG
CAAGGCGCATTAGCATCATTAAAAAAATAATAGATAATAATATAA
Length of 3' end seq. 275
Connected seq. ID VSI112P
Connected seq. 
>VSI112P.Seq
TTCCCACCATGTGGTGAATTGGCCTCACCAATCATGCATTTTGATAATGTTACTTTTTCA
TACAGCGGTAAAGAAGCAGATGTTTTATATAGAAATTTAGATTTAGCTATCGATTTAGAC
TCTAGAATTGCACTTGTTGGTCCAAATGGTGCTGGTAAAAGTACTTTATTGAAGCTTATG
GTCGGTCAAATCTCACCAACTCAAGGTTTCATTAAAAAACATAGTCATCTTAAAATGGCT
CGTTATCATCAACATGCTCATGAAGTTTTAGATCTCACCGCTACTCCATTGGATTTTGTT
CGTTCTAAATTTGCTCATATGAATAAAGATACCGAAGAATGGCGTCGTGAAATTGGTAGA
TTTGGTGTTACTGGTAAAGCTCAAACTGAAGCTATTGGTTGTATGTCTGATGGTATTAAA
TCTCGTTTAATTTTTCTGTCTTATGGCTCTCGAAAATCCACATCTTTTATTACTTGATGA
ACCAACAAATCATTTAGATATGGA----------TATTACTTGATGAACCAACAAATCAT
TTAGATATGGAATGTATTGATAGTTTAGCTTTGGCCATCAATTCTTTCCCCGGAGGCATG
ATATTAGTATCTCACGATTTTAGATTAATCTCTCAAGTTGCAAAAGAAATTTGGGTTTGT
GATAATAAAACTATTACTAAATGGGCAGGTGATATCACAAGCTATAAGAATCACTTAAAG
GCTCAAATGAGAGATCTCACAAAGCAAGGCGCATTAGCATCATTAAAAAAATAATAGATA
ATAATATAA
Length of connected seq. 779
Full length Seq ID VSI112E
Full length Seq. 
>VSI112E.Seq
TTCCCACCATGTGGTGAATTGGCCTCACCAATCATGCATTTTGATAATGTTACTTTTTCA
TACAGCGGTAAAGAAGCAGATGTTTTATATAGAAATTTAGATTTAGCTATCGATTTAGAC
TCTAGAATTGCACTTGTTGGTCCAAATGGTGCTGGTAAAAGTACTTTATTGAAGCTTATG
GTCGGTCAAATCTCACCAACTCAAGGTTTCATTAAAAAACATAGTCATCTTAAAATGGCT
CGTTATCATCAACATGCTCATGAAGTTTTAGATCTCACCGCTACTCCATTGGATTTTGTT
CGTTCTAAATTTGCTCATATGAATAAAGATACCGAAGAATGGCGTCGTGAAATTGGTAGA
TTTGGTGTTACTGGTAAAGCTCAAACTGAAGCTATTGGTTGTATGTCTGATGGTATTAAA
TCTCGTTTAATTTTTCTGTCTTATGGCTCTCGAAAATCCACATCTTTTATTACTTGATGA
ACCAACAAATCATTTAGATATGGAATGTATTGATAGTTTAGCTTTGGCCATCAATTCTTT
CCCCGGAGGCATGATATTAGTATCTCACGATTTTAGATTAATCTCTCAAGTTGCAAAAGA
AATTTGGGTTTGTGATAATAAAACTATTACTAAATGGGCAGGTGATATCACAAGCTATAA
GAATCACTTAAAGGCTCAAATGAGAGATCTCACAAAGCAAGGCGCATTAGCATCATTAAA
AAAATAATAGATAATAATATAA
Length of full length seq. 742