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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSH854
Library VS
(Link to library)
Clone ID VSH854
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VSH854P
(Link to Original site)
Representative DNA sequence 
>VSH854 (VSH854Q) /CSM/VS/VSH8-C/VSH854Q.Seq.d/
CAAAGAATCTATGGGTCATATCATGTCAAAAATTCATCATATCGCTTCTCTTAAAGGTGC
TGATTCTTACAGATTGGTCATTAATGAAGGTGTTTTAGGTCAACAATCTGTTAGATGGTT
ACATATTCATATCCTTGGTGGTAGACAAATGAATTGGCCACCAGGCTAATTTCAATTAAA
GAATTCTTCATTGTACCTATTTAAACCTTTTACTAATAATAGTAAAGAAAATTCACCATT
GGCACGTACATAATAAAAAAATATATCCTTTTAATGGATCACTAAAAAAAAAAXXXXXXX
XXXCAAAGAATCTATAGNTCATATCATGTCAAAAATTCATCATATCGCTTCTCTTAAAGG
TGCTGATTCTTACAGATTGGTCATTAATGAAGGTGTTTTAGGTCAACAATCTGTTAGATG
GTTACATATTCATATCCTTGGTGGTAGACAAATNAATTGGCCACCAGGCTAATTTCAATT
AAAGAATTCTTCATTGTACCTATTTAAACCTTTTACTAATAATAGTAAAGAAAATTCACC
ATTGGCACGTACATAATAAAAAAA
sequence update 2001. 3.22
Translated Amino Acid sequence 
knlwviscqkfiislllkvliltdwslmkvf*vnnlldgyifislvvdk*ighqanfn*R
ILHCTYLNLLLIIVKKIHHWHVHNKKIYPFNGSLKKK---

---KESIXHIMSKIHHIASLKGADSYRLVINEGVLGQQSVRWLHIHILGGRQXNWPPG*f
qlknsslylfkpftnnskensplart**k
Translated Amino Acid sequence (All Frames) 
Frame A:
qriygsyhvknssyrfs*rc*flqigh**rcfrstic*mvtysypww*tnelatrlisik
effivpi*tfy****rkftigtyiikkyillmdh*kk---

---qriyxsyhvknssyrfs*rc*flqigh**rcfrstic*mvtysypww*tnxlatrli
sikeffivpi*tfy****rkftigtyiikk

Frame B:
kesmghimskihhiaslkgadsyrlvinegvlgqqsvrwlhihilggrqmnwppg*fqlk
nsslylfkpftnnskensplart**knisf*witkkk---

---KESIXHIMSKIHHIASLKGADSYRLVINEGVLGQQSVRWLHIHILGGRQXNWPPG*f
qlknsslylfkpftnnskensplart**k

Frame C:
knlwviscqkfiislllkvliltdwslmkvf*vnnlldgyifislvvdk*ighqanfn*R
ILHCTYLNLLLIIVKKIHHWHVHNKKIYPFNGSLKKK---

---knl*xiscqkfiislllkvliltdwslmkvf*vnnlldgyifislvvdkxighqanf
n*rilhctylnllliivkkihhwhvhnkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSH854 (VSH854Q) /CSM/VS/VSH8-C/VSH854Q.Seq.d/                        519   e-146
VSE744 (VSE744Q) /CSM/VS/VSE7-B/VSE744Q.Seq.d/                        519   e-146
VSB884 (VSB884Q) /CSM/VS/VSB8-D/VSB884Q.Seq.d/                        519   e-146
VSB635 (VSB635Q) /CSM/VS/VSB6-B/VSB635Q.Seq.d/                        519   e-146
VHP367 (VHP367Q) /CSM/VH/VHP3-C/VHP367Q.Seq.d/                        519   e-146
SSM528 (SSM528Q) /CSM/SS/SSM5-B/SSM528Q.Seq.d/                        519   e-146
SSM293 (SSM293Q) /CSM/SS/SSM2-D/SSM293Q.Seq.d/                        519   e-146
SSL530 (SSL530Q) /CSM/SS/SSL5-B/SSL530Q.Seq.d/                        519   e-146
SSK724 (SSK724Q) /CSM/SS/SSK7-A/SSK724Q.Seq.d/                        519   e-146
SSK684 (SSK684Q) /CSM/SS/SSK6-D/SSK684Q.Seq.d/                        519   e-146
own update 2003. 1.10
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU051944) Dictyostelium discoideum slug cDNA, clone SSC443.      504   e-145  2
(C89678) Dictyostelium discoideum slug cDNA, clone SSA608.        504   e-145  2
(AU076312) Dictyostelium discoideum slug cDNA, clone SSA116.      504   e-145  2
(AU038148) Dictyostelium discoideum slug cDNA, clone SSH331.      504   e-145  2
(C90113) Dictyostelium discoideum slug cDNA, clone SSI106.        504   e-145  2
(AU038826) Dictyostelium discoideum slug cDNA, clone SSL530.      504   e-145  2
(AU039028) Dictyostelium discoideum slug cDNA, clone SSM293.      504   e-145  2
(AU038255) Dictyostelium discoideum slug cDNA, clone SSH478.      504   e-145  2
(AU038258) Dictyostelium discoideum slug cDNA, clone SSH481.      504   e-145  2
(AU038265) Dictyostelium discoideum slug cDNA, clone SSH491.      504   e-145  2
dna update 2009. 7. 1
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q23921) RecName: Full=Protein pkiA; AltName: Full=Protein DD-1;...   119   7e-26
U61986_1(U61986|pid:none) Dictyostelium discoideum PkiA (pkiA) m...    93   5e-18
(Q8SQ21) RecName: Full=Histidine triad nucleotide-binding protei...    65   9e-10
(Q9BX68) RecName: Full=Histidine triad nucleotide-binding protei...    65   9e-10
(Q9D0S9) RecName: Full=Histidine triad nucleotide-binding protei...    65   9e-10
BC135760_1(BC135760|pid:none) Xenopus tropicalis cDNA clone IMAG...    65   9e-10
AF085236_1(AF085236|pid:none) Homo sapiens protein kinase C inhi...    65   9e-10
BC068885_1(BC068885|pid:none) Xenopus laevis hypothetical protei...    65   2e-09
CR847821_2(CR847821|pid:none) Zebrafish DNA sequence from clone ...    64   2e-09
AK222582_1(AK222582|pid:none) Homo sapiens mRNA for PKCI-1-relat...    62   7e-09
protein update 2009. 3.24
PSORT 

 psg: 0.91  gvh: 0.34  alm: 0.35  top: 0.77  tms: 0.00  mit: 0.33  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 28.0 %: mitochondrial
	 24.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for VSH854 is cyt
5' end seq. ID VSH854F
5' end seq. 
>VSH854F.Seq
CAAAGAATCTATGGGTCATATCATGTCAAAAATTCATCATATCGCTTCTCTTAAAGGTGC
TGATTCTTACAGATTGGTCATTAATGAAGGTGTTTTAGGTCAACAATCTGTTAGATGGTT
ACATATTCATATCCTTGGTGGTAGACAAATGAATTGGCCACCAGGCTAATTTCAATTAAA
GAATTCTTCATTGTACCTATTTAAACCTTTTACTAATAATAGTAAAGAAAATTCACCATT
GGCACGTACATAATAAAAAAATATATCCTTTTAATGGATCACTAAAAAAAAAA-------
---
Length of 5' end seq. 293
3' end seq. ID VSH854Z
3' end seq. 
>VSH854Z.Seq
----------CAAAGAATCTATAGNTCATATCATGTCAAAAATTCATCATATCGCTTCTC
TTAAAGGTGCTGATTCTTACAGATTGGTCATTAATGAAGGTGTTTTAGGTCAACAATCTG
TTAGATGGTTACATATTCATATCCTTGGTGGTAGACAAATNAATTGGCCACCAGGCTAAT
TTCAATTAAAGAATTCTTCATTGTACCTATTTAAACCTTTTACTAATAATAGTAAAGAAA
ATTCACCATTGGCACGTACATAATAAAAAAA
Length of 3' end seq. 261
Connected seq. ID VSH854P
Connected seq. 
>VSH854P.Seq
CAAAGAATCTATGGGTCATATCATGTCAAAAATTCATCATATCGCTTCTCTTAAAGGTGC
TGATTCTTACAGATTGGTCATTAATGAAGGTGTTTTAGGTCAACAATCTGTTAGATGGTT
ACATATTCATATCCTTGGTGGTAGACAAATGAATTGGCCACCAGGCTAATTTCAATTAAA
GAATTCTTCATTGTACCTATTTAAACCTTTTACTAATAATAGTAAAGAAAATTCACCATT
GGCACGTACATAATAAAAAAATATATCCTTTTAATGGATCACTAAAAAAAAAA-------
---CAAAGAATCTATAGNTCATATCATGTCAAAAATTCATCATATCGCTTCTCTTAAAGG
TGCTGATTCTTACAGATTGGTCATTAATGAAGGTGTTTTAGGTCAACAATCTGTTAGATG
GTTACATATTCATATCCTTGGTGGTAGACAAATNAATTGGCCACCAGGCTAATTTCAATT
AAAGAATTCTTCATTGTACCTATTTAAACCTTTTACTAATAATAGTAAAGAAAATTCACC
ATTGGCACGTACATAATAAAAAAA
Length of connected seq. 554
Full length Seq ID -
Full length Seq. -
Length of full length seq. -