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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSH706
Library VS
(Link to library)
Clone ID VSH706
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VSH706E
(Link to Original site)
Representative DNA sequence 
>VSH706 (VSH706Q) /CSM/VS/VSH7-A/VSH706Q.Seq.d/
GACCCACGACGTCCGCGGNACGCGTGGGTGTTGATGCAAATGGTATCTTAAATGTATCAG
CTGAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTC
TTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATG
AACAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTA
AAAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTA
TTGAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGG
ATGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAAC
TCTATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATT
CACCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAA
ACATCTCTATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAATCAATAAAGCTTAC
TCATANATAGACACA
sequence update 2001. 3.25
Translated Amino Acid sequence 
PTTSAXRVGVDANGILNVSAEDKSTGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDE
QQKDRVESKNKLENYAFTVKNSIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKD
EYEDKMKALEAVVNPIMSKLYQEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseen
isik*is*kiis**nq*sllixrh
Translated Amino Acid sequence (All Frames) 
Frame A:
dprrprxawvlmqmvs*myqlkinqlvtnkrlqlpmikvvfqkkklkkwllmqrnsnnkm
nnkkivlnqrinwkimhsllkiqlkmkklqpkfqiqinqllnlklkvysng*nqikpqkr
mnmkir*kp*kqlsiqs*vnsikkevchkvvvcqvvcqmihqrvqttk*tn*idgifqrk
tslsnrflkk**vskinkaysxidt


Frame B:
thdvrgtrgc*ckwylkcis*r*inw*qtkdynyq**rssfkrrn*kngc*creiqttr*
ttkrsc*ike*igklcihc*kfn*r*kscsqnfrfr*iny*i*n*kctqmvriksnrrkg
*i*r*deslrsscqsnhe*tlsrrryatrwwyarwyvk*ftkefkqqsrrirlteffrgk
hlyqidflknnklvksiklthx*t


Frame C:
PTTSAXRVGVDANGILNVSAEDKSTGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDE
QQKDRVESKNKLENYAFTVKNSIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKD
EYEDKMKALEAVVNPIMSKLYQEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseen
isik*is*kiis**nq*sllixrh
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSH706 (VSH706Q) /CSM/VS/VSH7-A/VSH706Q.Seq.d/                       1207   0.0
CHI753 (CHI753Q) /CSM/CH/CHI7-C/CHI753Q.Seq.d/                       1152   0.0
VHP873 (VHP873Q) /CSM/VH/VHP8-D/VHP873Q.Seq.d/                       1142   0.0
VHA303 (VHA303Q) /CSM/VH/VHA3-A/VHA303Q.Seq.d/                       1142   0.0
SHC753 (SHC753Q) /CSM/SH/SHC7-C/SHC753Q.Seq.d/                       1142   0.0
CHS341 (CHS341Q) /CSM/CH/CHS3-B/CHS341Q.Seq.d/                       1142   0.0
CHM310 (CHM310Q) /CSM/CH/CHM3-A/CHM310Q.Seq.d/                       1142   0.0
CHI848 (CHI848Q) /CSM/CH/CHI8-B/CHI848Q.Seq.d/                       1142   0.0
CHI502 (CHI502Q) /CSM/CH/CHI5-A/CHI502Q.Seq.d/                       1142   0.0
CHI250 (CHI250Q) /CSM/CH/CHI2-C/CHI250Q.Seq.d/                       1142   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU267848) Dictyostelium discoideum vegetative cDNA clone:VS...  1160   0.0    1
(BJ380878) Dictyostelium discoideum cDNA clone:ddc39j13, 3' ...  1152   0.0    1
(BJ446443) Dictyostelium discoideum cDNA clone:ddv62b20, 3' ...  1142   0.0    1
(BJ446297) Dictyostelium discoideum cDNA clone:ddv62e08, 3' ...  1142   0.0    1
(BJ445794) Dictyostelium discoideum cDNA clone:ddv60m14, 3' ...  1142   0.0    1
(BJ445492) Dictyostelium discoideum cDNA clone:ddv59j16, 3' ...  1142   0.0    1
(BJ442861) Dictyostelium discoideum cDNA clone:ddv51n04, 3' ...  1142   0.0    1
(BJ442410) Dictyostelium discoideum cDNA clone:ddv49e20, 3' ...  1142   0.0    1
(BJ442220) Dictyostelium discoideum cDNA clone:ddv49o01, 3' ...  1142   0.0    1
(BJ441373) Dictyostelium discoideum cDNA clone:ddv46c15, 3' ...  1142   0.0    1
dna update 2009. 6.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   257   1e-67
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   257   1e-67
AF033852_1(AF033852|pid:none) Spinacia oleracea cytosolic heat s...   172   5e-42
FB790108_1(FB790108|pid:none) Sequence 9381 from Patent WO200803...   171   2e-41
(P24629) RecName: Full=Heat shock cognate 70 kDa protein 1;  &FB...   170   2e-41
AY253326_1(AY253326|pid:none) Nicotiana tabacum heat shock prote...   169   7e-41
(Q54BE0) RecName: Full=Heat shock cognate 70 kDa protein 3;           168   9e-41
(P22953) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   168   1e-40
AC147536_23(AC147536|pid:none) Medicago truncatula clone mth2-9l...   168   1e-40
AY830127_1(AY830127|pid:none) Medicago sativa heat shock protein...   168   1e-40
protein update 2009. 3.24
PSORT 

 psg: 0.78  gvh: 0.35  alm: 0.53  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VSH706 is cyt
5' end seq. ID VSH706F
5' end seq. 
>VSH706F.Seq
GACCCACGACGTCCGCGGNACGCGTGGGTGTTGATGCAAATGGTATCTTAAATGTATCAG
CTGAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTC
TTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATG
AACAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTA
AAAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTA
TTGAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGG
ATGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAAC
TCTATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATT
CACCAAAGAGTTCAAA----------
Length of 5' end seq. 496
3' end seq. ID VSH706Z
3' end seq. 
>VSH706Z.Seq
----------GTGTTGATGCAAATGGTATCTTAAATGTATCAGCTGAAGATAAATCAACT
GGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTTTCAAAAGAAGAAATT
GAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAACAACAAAAAGATCGT
GTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAAATTCAATTAAAGAT
GAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATTGAATCTGAAACTGAA
AGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATGAATATGAAGATAAG
ATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCTATCAAGAAGGAGGT
ATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCACCAAAGAGTTCAAAC
AACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAACATCTCTATCAAATAG
ATTTCTTAAAAAATAATAAGTTAGTAAAATCAATAAAGCTTACTCATANATAGACACA
Length of 3' end seq. 588
Connected seq. ID VSH706P
Connected seq. 
>VSH706P.Seq
GACCCACGACGTCCGCGGNACGCGTGGGTGTTGATGCAAATGGTATCTTAAATGTATCAG
CTGAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTC
TTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATG
AACAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTA
AAAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTA
TTGAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGG
ATGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAAC
TCTATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATT
CACCAAAGAGTTCAAA----------GTGTTGATGCAAATGGTATCTTAAATGTATCAGC
TGAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCT
TTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGA
ACAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAA
AAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTAT
TGAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGA
TGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACT
CTATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTC
ACCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAA
CATCTCTATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAATCAATAAAGCTTACT
CATANATAGACACA
Length of connected seq. 1084
Full length Seq ID VSH706E
Full length Seq. 
>VSH706E.Seq
GACCCACGACGTCCGCGGNACGCGTGGGTGTTGATGCAAATGGTATCTTAAATGTATCAG
CTGAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTC
TTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATG
AACAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTA
AAAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTA
TTGAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGG
ATGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAAC
TCTATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATT
CACCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAA
ACATCTCTATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAATCAATAAAGCTTAC
TCATANATAGACACA
Length of full length seq. 615