>AHA647 (AHA647Q) /CSM/AH/AHA6-B/AHA647Q.Seq.d/
ATATTTACATATTTTTAAAAAATGGCAACAATTGAAGATACTTANAAAGATAATAACAAT
CCAATTTTAGAACGTAAAAAAGCTCCAAATAGATTATTTGTAGAAGAAGCAATTAATGAT
GATAATTCAGTAGTTACATTAAATCCAGAGACAATGGATCAATTACAATTCTTTAGAGGT
GACACACTTTTAATTAAAGGTAAAAAGAGAAGAGATACCGTTTGTATTGTATTGTCAGAT
CCAACCATCGATCCATCAAAGATCCGTATGAATAAAGTTGTAAGAAATAATCTTCGTGTT
AGATTAGGTGACATGATTTCAGTTCACCAATGTTCAGATGTTAAATATGGCAAACGTATT
CATGTGTTACCAATTGATGATACCATAGAAGGTCTCAGTGGCAATCTCTTTGATCTTTAC
CTCAAACCATACTTTTTAGAAGCCTACCGTCCAGTCCGTAAGGGTGACTTATTTTTAGTA
CGTGGTGGTATGAGAGCCGTAGAATTTAAAGTTGTCGAAACCGACCCAGGTGAATATTGT
ATCGTTGCACCAGAGACATTTATACATTGTGAAGGTGAGGCAGTCAAGAGAGXXXXXXXX
XXTGTGTTTTGTTNTTTGATGAGTTGGATAGTATTGCACGTTTTANAGGTTCATCACAAG
GTGATGCTGGTGGTGCTGGTGATANAGTTATCAATCAAATTTTAACTGAAATGGATGGTA
TGAACGCCAANAANAACGTTTTCATCATTGGNGNTACCAATAGACCAGATATCATCGATC
CAGNTATCCTCCGTCCAGGTNGTTTAGATCAACTCATTTACATTCCATTACCAGATTTAC
CATTTNGNGTTGCCATTTTCAAGGNTTGTTTCAATAAATCNCCAGTCGCCAAGGATGTAG
ATTTANAATTTTTGGGTCAAAANACTCAAGGTTTNTTTGGNGCAGATTTAACTGAAATTT
GTCAACGNGCCTGTAAATTAGCTATTNGTGAATCCATTGAAAAGGATATTGAATCCNCCA
AAGCCCGTCAANAATTTGGTGATACCAANATGGAANATGACTNTGTCGATCCAGNACCAG
AAATTACTNGTGATCACTTCCAANAAGCCATGAGATNTGCTCGTCGTTCTGNATCCGATA
ATGATATTAGAAAATATGAAAGCTTTGCTCAAACTTTAGTTCAATCAANAGGTNTTGGTA
ATAATTTCAAATTCCCAGATCAAGAATNTTCAGGTCAATTCA

iftyf*KMATIEDTXKDNNNPILERKKAPNRLFVEEAINDDNSVVTLNPETMDQLQFFRG
DTLLIKGKKRRDTVCIVLSDPTIDPSKIRMNKVVRNNLRVRLGDMISVHQCSDVKYGKRI
HVLPIDDTIEGLSGNLFDLYLKPYFLEAYRPVRKGDLFLVRGGMRAVEFKVVETDPGEYC
IVAPETFIHCEGEAVKR---

---CVLXFDELDSIARFXGSSQGDAGGAGDXVINQILTEMDGMNAXXNVFIIGXTNRPDI
IDPXILRPGXLDQLIYIPLPDLPFXVAIFKXCFNKSPVAKDVDLXFLGQXTQGXFGADLT
EICQRACKLAIXESIEKDIESXKARQXFGDTXMEXDXVDPXPEITXDHFQXAMRXARRSX
SDNDIRKYESFAQTLVQSXGXGNNFKFPDQEXSGQF

Frame A:
iftyf*KMATIEDTXKDNNNPILERKKAPNRLFVEEAINDDNSVVTLNPETMDQLQFFRG
DTLLIKGKKRRDTVCIVLSDPTIDPSKIRMNKVVRNNLRVRLGDMISVHQCSDVKYGKRI
HVLPIDDTIEGLSGNLFDLYLKPYFLEAYRPVRKGDLFLVRGGMRAVEFKVVETDPGEYC
IVAPETFIHCEGEAVKR---

---CVLXFDELDSIARFXGSSQGDAGGAGDXVINQILTEMDGMNAXXNVFIIGXTNRPDI
IDPXILRPGXLDQLIYIPLPDLPFXVAIFKXCFNKSPVAKDVDLXFLGQXTQGXFGADLT
EICQRACKLAIXESIEKDIESXKARQXFGDTXMEXDXVDPXPEITXDHFQXAMRXARRSX
SDNDIRKYESFAQTLVQSXGXGNNFKFPDQEXSGQF

Frame B:
ylhifkkwqqlkilxkiitiqf*nvkklqidyl*kkqlmmiiq*lh*iqrqwinynslev
thf*lkvkreeipfvlycqiqpsihqrsv*ikl*eiifvld*vt*fqftnvqmlnmanvf
mcyqlmip*kvsvaisliftsnhtf*kptvqsvrvtyf*yvvv*ep*nlklskptqvniv
slhqrhlyivkvrqsre---

---vfcxlmswivlhvlxvhhkvmlvvlvixlsikf*lkwmv*tpxxtfsslxxpidqis
siqxssvqvv*insftfhyqiyhxxlpfsrxvsinxqsprm*ixnfwvkxlkvxlxqi*l
kfvnxpvn*lxvnplkrilnppkpvxnlvipxwxmtxsiqxqkllvitsxkp*dxlvvlx
pimilenmkallkl*fnqxvlviisnsqiknxqvns

Frame C:
iyiflkngnn*rylxr**qsnfrt*kssk*iicrrsn****fssyiksrdngsitil*r*
htfn*r*kekryrlycivrsnhrsikdpye*sckk*ssc*ir*hdfsspmfrc*iwqtys
cvtn**yhrrsqwqsl*slpqtilfrslpssp*g*lifstwwyesrri*scrnrpr*ily
rctrdiytl*r*gsqe---

---cfvx**vg*yctfxrfitr*cwwcw*xsyqsnfn*ngwyerqxxrfhhwxyq*tryh
rsxyppsrxfrsthlhsitrftixxchfqglfq*ixsrqgcrfxifgskxsrfxwxrfn*
nlstxl*isyx*ih*kgy*ixqspsxiw*yqxgx*lcrsxtrnyx*slpxshexcssfxi
r**y*ki*klcsnfssixrxw**fqiprsrxfrsi

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AHA647 (AHA647Q) /CSM/AH/AHA6-B/AHA647Q.Seq.d/                       2206   0.0
AFK847 (AFK847Q) /CSM/AF/AFK8-B/AFK847Q.Seq.d/                       1164   0.0
VHB764 (VHB764Q) /CSM/VH/VHB7-C/VHB764Q.Seq.d/                       1162   0.0
AHQ207 (AHQ207Q) /CSM/AH/AHQ2-A/AHQ207Q.Seq.d/                       1162   0.0
AHO210 (AHO210Q) /CSM/AH/AHO2-A/AHO210Q.Seq.d/                       1162   0.0
AHL126 (AHL126Q) /CSM/AH/AHL1-B/AHL126Q.Seq.d/                       1162   0.0
AHI502 (AHI502Q) /CSM/AH/AHI5-A/AHI502Q.Seq.d/                       1162   0.0
AHE244 (AHE244Q) /CSM/AH/AHE2-B/AHE244Q.Seq.d/                       1162   0.0
AHB113 (AHB113Q) /CSM/AH/AHB1-A/AHB113Q.Seq.d/                       1162   0.0
AHA242 (AHA242Q) /CSM/AH/AHA2-B/AHA242Q.Seq.d/                       1162   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U83085|U83085.2 Dictyostelium discoideum cell division cycle protein 48 (cdcD) mRNA, complete cds.  1160   0.0    1
BI505199|BI505199.1 BB170019B10G02.5 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170019B10G02 5', mRNA sequence.    62   2e-22  5
CB814564|CB814564.1 USDA-FP_100042 Adult Alate Brown Citrus Aphid Toxoptera citricida cDNA clone WHWTC-01_D12 5', mRNA sequence.   103   6e-18  1
BE579889|BE579889.1 kq34a03.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to WP:C41C4.8 CE05402 TRANSITIONAL ENDOPLASMIC RETICULUM ATPASE HOMOLOG 2 ;, mRNA sequence.    58   8e-18  3
U20213|U20213.1 Glycine max valosin-containing protein mRNA, complete cds.    58   1e-16  4
AL440049|AL440049.1 T7 end of clone BD0AA009F04 of library BD0AA from strain CBS 94 of  Candida tropicalis.    90   2e-16  2
CF588183|CF588183.1 USDA-FP_121400-098 Acyrthosiphon pisum, Pea Aphid Acyrthosiphon pisum cDNA clone WHAP-011_E12 5', mRNA sequence.    84   2e-16  2
BI504030|BI504030.1 BB170028B20C09.5 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170028B20C09 5', mRNA sequence.    68   3e-15  4
BQ134885|BQ134885.1 INIT1_1_D01.b1_A006 G5 trophont cDNA (INIT1) Ichthyophthirius multifiliis cDNA, mRNA sequence.    80   6e-15  2
AL440050|AL440050.1 T3 end of clone BD0AA009F04 of library BD0AA from strain CBS 94 of  Candida tropicalis.    84   1e-14  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U83085_1(U83085|pid:none) Dictyostelium discoideum cell division...   383   e-105
DQ515925_1(DQ515925|pid:none) Nicotiana tabacum putative spindle...   304   4e-81
AL162751_7(AL162751|pid:none) Arabidopsis thaliana DNA chromosom...   303   6e-81
FB781796_1(FB781796|pid:none) Sequence 1069 from Patent WO200803...   300   7e-80
EU606206_1(EU606206|pid:none) Dimocarpus longan cell division cy...   300   7e-80
FB781494_1(FB781494|pid:none) Sequence 767 from Patent WO2008034...   299   1e-79
GM966461_1(GM966461|pid:none) Sequence 1793 from Patent WO200814...   298   2e-79
EU961444_1(EU961444|pid:none) Zea mays clone 235510 unknown mRNA.     298   2e-79
FB905989_1(FB905989|pid:none) Sequence 125262 from Patent WO2008...   298   3e-79
FB781564_1(FB781564|pid:none) Sequence 837 from Patent WO2008034...   298   3e-79

 psg: 0.75  gvh: 0.33  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.07  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 40.0 %: nuclear
	  8.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for AHA647 is cyt

>AHA647F.Seq
ATATTTACATATTTTTAAAAAATGGCAACAATTGAAGATACTTANAAAGATAATAACAAT
CCAATTTTAGAACGTAAAAAAGCTCCAAATAGATTATTTGTAGAAGAAGCAATTAATGAT
GATAATTCAGTAGTTACATTAAATCCAGAGACAATGGATCAATTACAATTCTTTAGAGGT
GACACACTTTTAATTAAAGGTAAAAAGAGAAGAGATACCGTTTGTATTGTATTGTCAGAT
CCAACCATCGATCCATCAAAGATCCGTATGAATAAAGTTGTAAGAAATAATCTTCGTGTT
AGATTAGGTGACATGATTTCAGTTCACCAATGTTCAGATGTTAAATATGGCAAACGTATT
CATGTGTTACCAATTGATGATACCATAGAAGGTCTCAGTGGCAATCTCTTTGATCTTTAC
CTCAAACCATACTTTTTAGAAGCCTACCGTCCAGTCCGTAAGGGTGACTTATTTTTAGTA
CGTGGTGGTATGAGAGCCGTAGAATTTAAAGTTGTCGAAACCGACCCAGGTGAATATTGT
ATCGTTGCACCAGAGACATTTATACATTGTGAAGGTGAGGCAGTCAAGAGAGNNNNNNNN
NN

>AHA647Z.Seq
NNNNNNNNNNTGTGTTTTGTTNTTTGATGAGTTGGATAGTATTGCACGTTTTANAGGTTC
ATCACAAGGTGATGCTGGTGGTGCTGGTGATANAGTTATCAATCAAATTTTAACTGAAAT
GGATGGTATGAACGCCAANAANAACGTTTTCATCATTGGNGNTACCAATAGACCAGATAT
CATCGATCCAGNTATCCTCCGTCCAGGTNGTTTAGATCAACTCATTTACATTCCATTACC
AGATTTACCATTTNGNGTTGCCATTTTCAAGGNTTGTTTCAATAAATCNCCAGTCGCCAA
GGATGTAGATTTANAATTTTTGGGTCAAAANACTCAAGGTTTNTTTGGNGCAGATTTAAC
TGAAATTTGTCAACGNGCCTGTAAATTAGCTATTNGTGAATCCATTGAAAAGGATATTGA
ATCCNCCAAAGCCCGTCAANAATTTGGTGATACCAANATGGAANATGACTNTGTCGATCC
AGNACCAGAAATTACTNGTGATCACTTCCAANAAGCCATGAGATNTGCTCGTCGTTCTGN
ATCCGATAATGATATTAGAAAATATGAAAGCTTTGCTCAAACTTTAGTTCAATCAANAGG
TNTTGGTAATAATTTCAAATTCCCAGATCAAGAATNTTCAGGTCAATTCA

>AHA647P.Seq
ATATTTACATATTTTTAAAAAATGGCAACAATTGAAGATACTTANAAAGATAATAACAAT
CCAATTTTAGAACGTAAAAAAGCTCCAAATAGATTATTTGTAGAAGAAGCAATTAATGAT
GATAATTCAGTAGTTACATTAAATCCAGAGACAATGGATCAATTACAATTCTTTAGAGGT
GACACACTTTTAATTAAAGGTAAAAAGAGAAGAGATACCGTTTGTATTGTATTGTCAGAT
CCAACCATCGATCCATCAAAGATCCGTATGAATAAAGTTGTAAGAAATAATCTTCGTGTT
AGATTAGGTGACATGATTTCAGTTCACCAATGTTCAGATGTTAAATATGGCAAACGTATT
CATGTGTTACCAATTGATGATACCATAGAAGGTCTCAGTGGCAATCTCTTTGATCTTTAC
CTCAAACCATACTTTTTAGAAGCCTACCGTCCAGTCCGTAAGGGTGACTTATTTTTAGTA
CGTGGTGGTATGAGAGCCGTAGAATTTAAAGTTGTCGAAACCGACCCAGGTGAATATTGT
ATCGTTGCACCAGAGACATTTATACATTGTGAAGGTGAGGCAGTCAAGAGAG--------
--TGTGTTTTGTTNTTTGATGAGTTGGATAGTATTGCACGTTTTANAGGTTCATCACAAG
GTGATGCTGGTGGTGCTGGTGATANAGTTATCAATCAAATTTTAACTGAAATGGATGGTA
TGAACGCCAANAANAACGTTTTCATCATTGGNGNTACCAATAGACCAGATATCATCGATC
CAGNTATCCTCCGTCCAGGTNGTTTAGATCAACTCATTTACATTCCATTACCAGATTTAC
CATTTNGNGTTGCCATTTTCAAGGNTTGTTTCAATAAATCNCCAGTCGCCAAGGATGTAG
ATTTANAATTTTTGGGTCAAAANACTCAAGGTTTNTTTGGNGCAGATTTAACTGAAATTT
GTCAACGNGCCTGTAAATTAGCTATTNGTGAATCCATTGAAAAGGATATTGAATCCNCCA
AAGCCCGTCAANAATTTGGTGATACCAANATGGAANATGACTNTGTCGATCCAGNACCAG
AAATTACTNGTGATCACTTCCAANAAGCCATGAGATNTGCTCGTCGTTCTGNATCCGATA
ATGATATTAGAAAATATGAAAGCTTTGCTCAAACTTTAGTTCAATCAANAGGTNTTGGTA
ATAATTTCAAATTCCCAGATCAAGAATNTTCAGGTCAATTCA