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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSH576
Library VS
(Link to library)
Clone ID VSH576
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14804-1
Original site URL
Representative seq. ID VSH576P
(Link to Original site)
Representative DNA sequence 
>VSH576 (VSH576Q) /CSM/VS/VSH5-D/VSH576Q.Seq.d/
AAAAGAAGCTAGTGTTTATCCTTTTAGAAATAATTAGAAAATGAATTTTGAATCGATTGA
ATTTATTACTTGTCCACCAAAACCAATGCCAAAGCCAAATAAGCCATCCAAACCATTAAA
GACTTCTTTAATTGCTTAAGCCAAACAAATCATATCTCTTCCAAGTGGTCTCTTTCTCTT
TTTCTTTCCTTAGACACCAACCATCATTGATGTAGTTTTGTTTATTTTTATTACCAACAT
AAAAAACAAAATAAAAATAAAAATAAAAAAAATTAAAAGAGATCAATAAGACAATATTTT
TTTAAAAAAAAAAXXXXXXXXXXAAAAGAAGCTAGTGTTTATCCTTTTAGAAATAATTAG
AAAATGAATTTTGAATCGATTGAATTTATTACTTGTCCACCAAAACCAATGCCAAAGCCA
AATAAGCCATCCAAACCATTAAAGACTTCTTTAATTGCTTAAGCCAAACAAATCATATCT
CTTCCAAGTGGTCTCTTTCTCTTTTTCTTTCCTTAGACACCAACCATCATTGATGTAGTT
TTGTTTATTTTTATTACCAACATAAAAAACAAAATAAAAATAAAAATAAAAAAAATTAAA
AGAGTATCAATAAGA
sequence update 2001. 3.22
Translated Amino Acid sequence 
krs*clsf*k*lenef*id*iyylstktnakak*aiqtikdffnclsqtnhisskwslsl
flsldtnhh*csfvyfyyqhkkqnknknkkn*KRSIRQYFFKKK---

---KKLVFILLEIIRK*ilnrlnlllvhqnqcqsqishpnh*rll*llkpnksylfqvvs
fsfsflrhqpslm*fclfllpt*ktk*k*k*kklkeyq*
Translated Amino Acid sequence (All Frames) 
Frame A:
krs*clsf*k*lenef*id*iyylstktnakak*aiqtikdffnclsqtnhisskwslsl
flsldtnhh*csfvyfyyqhkkqnknknkkn*KRSIRQYFFKKK---

---krs*clsf*k*lenef*id*iyylstktnakak*aiqtikdffnclsqtnhisskws
lslflsldtnhh*csfvyfyyqhkkqnknknkkn*ksink

Frame B:
keasvypfrnn*kmnfesiefitcppkpmpkpnkpskplktslia*akqiislpsglflf
ffp*tptiidvvlfifitniknkikikikkikrdq*dniflkkk---

---keasvypfrnn*kmnfesiefitcppkpmpkpnkpskplktslia*akqiislpsgl
flfffp*tptiidvvlfifitniknkikikikkikrvsir

Frame C:
kklvfilleiirk*ilnrlnlllvhqnqcqsqishpnh*rll*llkpnksylfqvvsfsf
sflrhqpslm*fclfllpt*ktk*k*k*kklkeinktiff*kk---

---KKLVFILLEIIRK*ilnrlnlllvhqnqcqsqishpnh*rll*llkpnksylfqvvs
fsfsflrhqpslm*fclfllpt*ktk*k*k*kklkeyq*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSH576 (VSH576Q) /CSM/VS/VSH5-D/VSH576Q.Seq.d/                        474   e-133
VHG295 (VHG295Q) /CSM/VH/VHG2-D/VHG295Q.Seq.d/                        474   e-133
VHK539 (VHK539Q) /CSM/VH/VHK5-B/VHK539Q.Seq.d/                         40   0.039
SHL719 (SHL719Q) /CSM/SH/SHL7-A/SHL719Q.Seq.d/                         40   0.039
AFN683 (AFN683Q) /CSM/AF/AFN6-D/AFN683Q.Seq.d/                         40   0.039
VFJ619 (VFJ619Q) /CSM/VF/VFJ6-A/VFJ619Q.Seq.d/                         38   0.15
SSD761 (SSD761Q) /CSM/SS/SSD7-C/SSD761Q.Seq.d/                         38   0.15
SLB178 (SLB178Q) /CSM/SL/SLB1-D/SLB178Q.Seq.d/                         38   0.15
SHE221 (SHE221Q) /CSM/SH/SHE2-A/SHE221Q.Seq.d/                         38   0.15
AFJ755 (AFJ755Q) /CSM/AF/AFJ7-C/AFJ755Q.Seq.d/                         38   0.15
own update 2004. 8. 6
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU267654) Dictyostelium discoideum vegetative cDNA clone:VS...   476   e-132  2
(AU267653) Dictyostelium discoideum vegetative cDNA clone:VS...   476   e-130  1
(BJ421815) Dictyostelium discoideum cDNA clone:ddv43m24, 5' ...   476   e-130  1
(ET698124) CHO_OF845xo03f1.ab1 CHO_OF Nicotiana tabacum geno...    46   8e-05  2
(BZ596741) SALK_095869.38.30.x Arabidopsis thaliana TDNA ins...    44   2e-04  2
(FL642346) TS15-A4 Reticulitermes flavipes symbiont library ...    40   0.001  2
(FL641038) TS00-A11 Reticulitermes flavipes symbiont library...    40   0.001  2
(CX903049) JGI_CAAM14066.fwd NIH_XGC_tropTe3 Xenopus (Silura...    40   0.001  2
(CX900954) JGI_CAAM9443.rev NIH_XGC_tropTe3 Xenopus (Siluran...    40   0.002  2
(AC226201) Zea mays chromosome 3 clone ZMMBBb-411F8; ZMMBBb0...    40   0.003  4
dna update 2009. 6.18
Homology vs Protein 

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3019103
Number of Hits to DB: 491,709,833
protein update 2009. 3.24
PSORT 

 psg: 0.64  gvh: -0.04  alm: 0.48  top: 0.53  tms: 0.00  mit: 0.47  mip: 0.00
 nuc: 0.45  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 80.0 %: nuclear
	  8.0 %: mitochondrial
	  8.0 %: cytoplasmic
	  4.0 %: endoplasmic reticulum

>> prediction for VSH576 is nuc
5' end seq. ID VSH576F
5' end seq. 
>VSH576F.Seq
AAAAGAAGCTAGTGTTTATCCTTTTAGAAATAATTAGAAAATGAATTTTGAATCGATTGA
ATTTATTACTTGTCCACCAAAACCAATGCCAAAGCCAAATAAGCCATCCAAACCATTAAA
GACTTCTTTAATTGCTTAAGCCAAACAAATCATATCTCTTCCAAGTGGTCTCTTTCTCTT
TTTCTTTCCTTAGACACCAACCATCATTGATGTAGTTTTGTTTATTTTTATTACCAACAT
AAAAAACAAAATAAAAATAAAAATAAAAAAAATTAAAAGAGATCAATAAGACAATATTTT
TTTAAAAAAAAAA----------
Length of 5' end seq. 313
3' end seq. ID VSH576Z
3' end seq. 
>VSH576Z.Seq
----------AAAAGAAGCTAGTGTTTATCCTTTTAGAAATAATTAGAAAATGAATTTTG
AATCGATTGAATTTATTACTTGTCCACCAAAACCAATGCCAAAGCCAAATAAGCCATCCA
AACCATTAAAGACTTCTTTAATTGCTTAAGCCAAACAAATCATATCTCTTCCAAGTGGTC
TCTTTCTCTTTTTCTTTCCTTAGACACCAACCATCATTGATGTAGTTTTGTTTATTTTTA
TTACCAACATAAAAAACAAAATAAAAATAAAAATAAAAAAAATTAAAAGAGTATCAATAA
GA
Length of 3' end seq. 292
Connected seq. ID VSH576P
Connected seq. 
>VSH576P.Seq
AAAAGAAGCTAGTGTTTATCCTTTTAGAAATAATTAGAAAATGAATTTTGAATCGATTGA
ATTTATTACTTGTCCACCAAAACCAATGCCAAAGCCAAATAAGCCATCCAAACCATTAAA
GACTTCTTTAATTGCTTAAGCCAAACAAATCATATCTCTTCCAAGTGGTCTCTTTCTCTT
TTTCTTTCCTTAGACACCAACCATCATTGATGTAGTTTTGTTTATTTTTATTACCAACAT
AAAAAACAAAATAAAAATAAAAATAAAAAAAATTAAAAGAGATCAATAAGACAATATTTT
TTTAAAAAAAAAA----------AAAAGAAGCTAGTGTTTATCCTTTTAGAAATAATTAG
AAAATGAATTTTGAATCGATTGAATTTATTACTTGTCCACCAAAACCAATGCCAAAGCCA
AATAAGCCATCCAAACCATTAAAGACTTCTTTAATTGCTTAAGCCAAACAAATCATATCT
CTTCCAAGTGGTCTCTTTCTCTTTTTCTTTCCTTAGACACCAACCATCATTGATGTAGTT
TTGTTTATTTTTATTACCAACATAAAAAACAAAATAAAAATAAAAATAAAAAAAATTAAA
AGAGTATCAATAAGA
Length of connected seq. 605
Full length Seq ID -
Full length Seq. -
Length of full length seq. -