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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSH575
Library VS
(Link to library)
Clone ID VSH575
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15814-1
Original site URL
Representative seq. ID VSH575E
(Link to Original site)
Representative DNA sequence 
>VSH575 (VSH575Q) /CSM/VS/VSH5-D/VSH575Q.Seq.d/
AAATTTTATACCAATCAAATAAAAATGTCTGCTTCAGTTAAATGTGGTGCTTGCGCTAAA
ACCGCCTATCCATTAGAATCAGTTGTTGCTAACAACAATTCATACCACAAGGGTTGTTTC
AAATGCTCAACTTGCAATTCTACTCTCAATGTCAAAACTTTCAAATCATTCGAAGGTAAA
CTCTATTGTCCAGTCCACACACCAAAAGTTTCAGCTACCGCTGTTACTGATTCAGTTGCC
TTAAAGAATGCCCTCAATGCACCAAAGAAAGTTGCTGAAGGTTTAGGTAATGCCCACAGA
GGTTTAGATGAAAAACCAAACATTGGTCTTGACTCAATGGCCACTGCTAATGCCCTCAAT
GCCCCAAAGAAGGTTGTTGAAGGTTTAGGTNATGTCCANAAAGGTATTGGTGGTAAACCA
ACCTACGCCGTATTTGGTGCTGATGGTCAACCAACTGGTGAACAACAAGAACAACAACAA
TACNCT
sequence update 2001. 3.25
Translated Amino Acid sequence 
KFYTNQIKMSASVKCGACAKTAYPLESVVANNNSYHKGCFKCSTCNSTLNVKTFKSFEGK
LYCPVHTPKVSATAVTDSVALKNALNAPKKVAEGLGNAHRGLDEKPNIGLDSMATANALN
APKKVVEGLGXVXKGIGGKPTYAVFGADGQPTGEQQEQQQYX
Translated Amino Acid sequence (All Frames) 
Frame A:
KFYTNQIKMSASVKCGACAKTAYPLESVVANNNSYHKGCFKCSTCNSTLNVKTFKSFEGK
LYCPVHTPKVSATAVTDSVALKNALNAPKKVAEGLGNAHRGLDEKPNIGLDSMATANALN
APKKVVEGLGXVXKGIGGKPTYAVFGADGQPTGEQQEQQQYX


Frame B:
nfipik*kcllqlnvvlalkppih*nqlllttihttrvvsnaqlaillsmsklsnhskvn
sivqsthqkfqlpllliqlp*rmpsmhqrkllkv*vmptev*mknqtlvltqwpllmpsm
pqrrllkv*vmsxkvlvvnqptpylvlmvnqlvnnknnnnt


Frame C:
ilyqsnknvcfs*mwclr*nrlsiriscc*qqfipqglfqmlnlqfysqcqnfqiirr*t
llssphtksfsyrcy*fsclkecpqctkesc*rfr*cpqrfr*ktkhws*lnghc*cpqc
pkegc*rfrxcpxryww*tnlrriwc*wstnw*ttrtttix
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ234 (VSJ234Q) /CSM/VS/VSJ2-B/VSJ234Q.Seq.d/                        946   0.0
VSJ176 (VSJ176Q) /CSM/VS/VSJ1-D/VSJ176Q.Seq.d/                        946   0.0
VSH575 (VSH575Q) /CSM/VS/VSH5-D/VSH575Q.Seq.d/                        946   0.0
VSH568 (VSH568Q) /CSM/VS/VSH5-C/VSH568Q.Seq.d/                        946   0.0
VSH152 (VSH152Q) /CSM/VS/VSH1-C/VSH152Q.Seq.d/                        946   0.0
VSI705 (VSI705Q) /CSM/VS/VSI7-A/VSI705Q.Seq.d/                        944   0.0
VSI675 (VSI675Q) /CSM/VS/VSI6-D/VSI675Q.Seq.d/                        932   0.0
VSI670 (VSI670Q) /CSM/VS/VSI6-C/VSI670Q.Seq.d/                        932   0.0
AHA121 (AHA121Q) /CSM/AH/AHA1-A/AHA121Q.Seq.d/                        926   0.0
FC-BE17 (FC-BE17Q) /CSM/FC/FC-BE/FC-BE17Q.Seq.d/                      922   0.0
own update 2003. 1.10
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(U97699) Dictyostelium discoideum LIM domain protein (CRP) m...   946   0.0    1
(AU269617) Dictyostelium discoideum vegetative cDNA clone:VS...   946   0.0    1
(AU269523) Dictyostelium discoideum vegetative cDNA clone:VS...   946   0.0    1
(AU269062) Dictyostelium discoideum vegetative cDNA clone:VS...   946   0.0    1
(AU267642) Dictyostelium discoideum vegetative cDNA clone:VS...   946   0.0    1
(AU266984) Dictyostelium discoideum vegetative cDNA clone:VS...   946   0.0    1
(BJ330316) Dictyostelium discoideum cDNA clone:dda31i05, 5' ...   946   0.0    1
(AU269108) Dictyostelium discoideum vegetative cDNA clone:VS...   944   0.0    1
(AU269061) Dictyostelium discoideum vegetative cDNA clone:VS...   801   0.0    2
(AU284605) Dictyostelium discoideum gamete cDNA clone:FC-BE1...   706   0.0    2
dna update 2009. 6.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(O60952) RecName: Full=LIM domain-containing protein E; AltName:...   259   3e-68
(Q8I7C3) RecName: Full=LIM and SH3 domain protein Lasp;  &AE0142...    75   6e-13
AJ294538_1(AJ294538|pid:none) Drosophila melanogaster mRNA for L...    75   6e-13
AE014296_2946(AE014296|pid:none) Drosophila melanogaster chromos...    75   6e-13
BC066574_1(BC066574|pid:none) Danio rerio zgc:77542, mRNA (cDNA ...    71   1e-11
BT053270_1(BT053270|pid:none) Medicago truncatula clone MTYFP_FQ...    71   1e-11
AM469183_1(AM469183|pid:none) Vitis vinifera contig VV78X221882....    71   1e-11
(Q5R5W0) RecName: Full=LIM and SH3 domain protein 1;          Sh...    70   2e-11
(Q3B7M5) RecName: Full=LIM and SH3 domain protein 1;          Sh...    70   2e-11
BC012460_1(BC012460|pid:none) Homo sapiens LIM and SH3 protein 1...    70   2e-11
protein update 2009. 3.24
PSORT 

 psg: 0.79  gvh: 0.45  alm: 0.50  top: 0.53  tms: 0.00  mit: 0.32  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.12  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 24.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for VSH575 is nuc
5' end seq. ID VSH575F
5' end seq. 
>VSH575F.Seq
AAATTTTATACCAATCAAATAAAAATGTCTGCTTCAGTTAAATGTGGTGCTTGCGCTAAA
ACCGCCTATCCATTAGAATCAGTTGTTGCTAACAACAATTCATACCACAAGGGTTGTTTC
AAATGCTCAACTTGCAATTCTACTCTCAATGTCAAAACTTTCAAATCATTCGAAGGTAAA
CTCTATTGTCCAGTCCACACACCAAAAGTTTCAGCTACCGCTGTTACTGATTCAGTTGCC
TTAAAGAATGCCCTCAATGCACCAAAGAAAGTTGCTGAAGGTTTAGGTAATGCCCACAGA
GGTTTAGATGAAAAACCAAACATTGGTCTTGACTCAATGGCCACTGCTAATGCCCTCAAT
GCCCCAAAGAAGGTTGTTGAAGGTTTAGGTAATGTCCAAAAAGGTATTGGTGGTAAACCA
ACCTACGCCGTATTTGGTGCTGATGGTCAACC----------
Length of 5' end seq. 452
3' end seq. ID VSH575Z
3' end seq. 
>VSH575Z.Seq
----------TTTAGGTTNTGTCCATAAAGGTATTGGTGGTAAACCAACCTACGCCGTAT
TTGGTGCTGATGGTCAACCAACTGGTGAACAACAAGAACAACAACAATACNCT
Length of 3' end seq. 103
Connected seq. ID VSH575P
Connected seq. 
>VSH575P.Seq
AAATTTTATACCAATCAAATAAAAATGTCTGCTTCAGTTAAATGTGGTGCTTGCGCTAAA
ACCGCCTATCCATTAGAATCAGTTGTTGCTAACAACAATTCATACCACAAGGGTTGTTTC
AAATGCTCAACTTGCAATTCTACTCTCAATGTCAAAACTTTCAAATCATTCGAAGGTAAA
CTCTATTGTCCAGTCCACACACCAAAAGTTTCAGCTACCGCTGTTACTGATTCAGTTGCC
TTAAAGAATGCCCTCAATGCACCAAAGAAAGTTGCTGAAGGTTTAGGTAATGCCCACAGA
GGTTTAGATGAAAAACCAAACATTGGTCTTGACTCAATGGCCACTGCTAATGCCCTCAAT
GCCCCAAAGAAGGTTGTTGAAGGTTTAGGTAATGTCCAAAAAGGTATTGGTGGTAAACCA
ACCTACGCCGTATTTGGTGCTGATGGTCAACC----------TTTAGGTTNTGTCCATAA
AGGTATTGGTGGTAAACCAACCTACGCCGTATTTGGTGCTGATGGTCAACCAACTGGTGA
ACAACAAGAACAACAACAATACNCT
Length of connected seq. 555
Full length Seq ID VSH575E
Full length Seq. 
>VSH575E.Seq
AAATTTTATACCAATCAAATAAAAATGTCTGCTTCAGTTAAATGTGGTGCTTGCGCTAAA
ACCGCCTATCCATTAGAATCAGTTGTTGCTAACAACAATTCATACCACAAGGGTTGTTTC
AAATGCTCAACTTGCAATTCTACTCTCAATGTCAAAACTTTCAAATCATTCGAAGGTAAA
CTCTATTGTCCAGTCCACACACCAAAAGTTTCAGCTACCGCTGTTACTGATTCAGTTGCC
TTAAAGAATGCCCTCAATGCACCAAAGAAAGTTGCTGAAGGTTTAGGTAATGCCCACAGA
GGTTTAGATGAAAAACCAAACATTGGTCTTGACTCAATGGCCACTGCTAATGCCCTCAAT
GCCCCAAAGAAGGTTGTTGAAGGTTTAGGTNATGTCCANAAAGGTATTGGTGGTAAACCA
ACCTACGCCGTATTTGGTGCTGATGGTCAACCAACTGGTGAACAACAAGAACAACAACAA
TACNCT
Length of full length seq. 486