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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSH527
Library VS
(Link to library)
Clone ID VSH527
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12720-1
Original site URL
Representative seq. ID VSH527P
(Link to Original site)
Representative DNA sequence 
>VSH527 (VSH527Q) /CSM/VS/VSH5-B/VSH527Q.Seq.d/
ATGTCAACAAGAGTACAAAAATCTGCAATTTTAAATGAAAACATAGACAAAGTATGGAAT
GTTTTACGTAATTTCGATTTCCCATCAAAAATCTTCCCAGTAATCGAAAGTTCAATTATT
GAAGGTGACTCAACACCAACCACTGTTGGAGCCATCCGTGTTTTAAAGTGGAAAACTGGT
GAAACCAATAAACAAAGATTATTAGAACTCTCTGATCTTTCACATAAAATCATTTATGAA
TTAATTGAAAGTGAACAAACTGCTGAAGTTACTGCCTACATTAGCACCATTAAATTAATT
CGTATTACTGAATCAAATCAAACCCTTTTGACTTGGGAAAGTGAATTTTCAGCAGATGTA
AAGAAGGATTTAATTAATTTCGAATCAAAATCAGTTTCCTTAAATTTACAAGATTTACAA
AAATTCTTTTCAAAATAAATTAAATTCTCTATTCTTTTAATTTTAAAAATTAANAANNAA
AXXXXXXXXXXATGTCAACAAGAGTACAAAAATCTGCAATTTTAAATGAAAACATAGACA
AAGTATGGAATGTTTTACGTAATTTCGATTTCCCATCAAAAATCTTCCCAGTAATCGAAA
GTTCAATTATTGAAGGTGACTCAACACCAACCACTGTTGGAGCCATCCGTGTTTTAAAGT
GGAAAACTGGTGAAACCAATAAACAAAGATTATTAGAACTCTCTGATCTTTCACATAAAA
TCATTTATGAATTAATTGAAAGTGAACAAACTGCTGAAGTTACTGCCTACATTAGCACCA
TTAAATTAATTCGTATTACTGAATCAAATCAAACCCTTTTGACTTGGGAAAGTGAATTTT
CAGCAGATGTAAAGAAGGATTTAATTAATTTCGAATCAAAATCAGTTTCCTTAAATTTAC
AAGATTTACAAAAATTCTTTTCAAAATAAATTAAATTACTCTATTCTTTTAATTTTAAAA
A
sequence update 2001. 3.22
Translated Amino Acid sequence 
mstrvqksailnenidkvwnvlrnfdfpskifpviessiiegdstpttvgairvlkwktg
etnkqrllelsdlshkiiyelieseqtaevtayistikliritesnqtlltwesefsadv
kkdlinfesksvslnlqdlqkffsk*IKFSILLILKIXXX---

---MSTRVQKSAILNENIDKVWNVLRNFDFPSKIFPVIESSIIEGDSTPTTVGAIRVLKW
KTGETNKQRLLELSDLSHKIIYELIESEQTAEVTAYISTIKLIRITESNQTLLTWESEFS
ADVKKDLINFESKSVSLNLQDLQKFFSK*ikllysfnfk
Translated Amino Acid sequence (All Frames) 
Frame A:
mstrvqksailnenidkvwnvlrnfdfpskifpviessiiegdstpttvgairvlkwktg
etnkqrllelsdlshkiiyelieseqtaevtayistikliritesnqtlltwesefsadv
kkdlinfesksvslnlqdlqkffsk*IKFSILLILKIXXX---

---MSTRVQKSAILNENIDKVWNVLRNFDFPSKIFPVIESSIIEGDSTPTTVGAIRVLKW
KTGETNKQRLLELSDLSHKIIYELIESEQTAEVTAYISTIKLIRITESNQTLLTWESEFS
ADVKKDLINFESKSVSLNLQDLQKFFSK*ikllysfnfk

Frame B:
cqqeyknlqf*mkt*tkygmfyvisishqkssq*skvqllkvtqhqpllepsvf*sgklv
kpinkdy*nslifhiksfmn*lkvnkllkllptlapln*fvllnqikpf*lgkvnfqqm*
rri*lisnqnqfp*iykiyknsfqnklnslff*f*klxxk---

---cqqeyknlqf*mkt*tkygmfyvisishqkssq*skvqllkvtqhqpllepsvf*sg
klvkpinkdy*nslifhiksfmn*lkvnkllkllptlapln*fvllnqikpf*lgkvnfq
qm*rri*lisnqnqfp*iykiyknsfqnklnysillilk

Frame C:
vnkstkicnfk*khrqsmecft*frfpiknlpsnrkfny*r*lntnhcwshpcfkvenw*
nq*tkiirtl*sft*nhl*in*k*tnc*syclh*hh*insyy*iksnpfdlgk*ifsrck
egfn*frikisflkftrftkilfkin*ilysfnfkn*xx---

---vnkstkicnfk*khrqsmecft*frfpiknlpsnrkfny*r*lntnhcwshpcfkve
nw*nq*tkiirtl*sft*nhl*in*k*tnc*syclh*hh*insyy*iksnpfdlgk*ifs
rckegfn*frikisflkftrftkilfkin*itlff*f*k
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSH527 (VSH527Q) /CSM/VS/VSH5-B/VSH527Q.Seq.d/                        932   0.0
VSH795 (VSH795Q) /CSM/VS/VSH7-D/VSH795Q.Seq.d/                        916   0.0
VSE874 (VSE874Q) /CSM/VS/VSE8-D/VSE874Q.Seq.d/                        916   0.0
AHC765 (AHC765Q) /CSM/AH/AHC7-C/AHC765Q.Seq.d/                        916   0.0
SSD805 (SSD805Q) /CSM/SS/SSD8-A/SSD805Q.Seq.d/                        914   0.0
VSC672 (VSC672Q) /CSM/VS/VSC6-C/VSC672Q.Seq.d/                        906   0.0
VSD151 (VSD151Q) /CSM/VS/VSD1-C/VSD151Q.Seq.d/                        902   0.0
VSB579 (VSB579Q) /CSM/VS/VSB5-D/VSB579Q.Seq.d/                        892   0.0
AHH891 (AHH891Q) /CSM/AH/AHH8-D/AHH891Q.Seq.d/                        860   0.0
VSF407 (VSF407Q) /CSM/VS/VSF4-A/VSF407Q.Seq.d/                        850   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU267986) Dictyostelium discoideum vegetative cDNA clone:VS...   817   0.0    1
(AU267578) Dictyostelium discoideum vegetative cDNA clone:VS...   817   0.0    1
(AU267577) Dictyostelium discoideum vegetative cDNA clone:VS...   817   0.0    1
(AU263447) Dictyostelium discoideum vegetative cDNA clone:VS...   817   0.0    1
(BJ349440) Dictyostelium discoideum cDNA clone:dda36b17, 3' ...   817   0.0    1
(BJ331646) Dictyostelium discoideum cDNA clone:dda36b17, 5' ...   817   0.0    1
(AU037573) Dictyostelium discoideum slug cDNA, clone SSD805.      815   0.0    1
(AU262716) Dictyostelium discoideum vegetative cDNA clone:VS...   807   0.0    1
(AU263557) Dictyostelium discoideum vegetative cDNA clone:VS...   803   0.0    1
(AU263556) Dictyostelium discoideum vegetative cDNA clone:VS...   803   0.0    1
dna update 2009. 6.17
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000393_3865(CP000393|pid:none) Trichodesmium erythraeum IMS101...    72   2e-11
BA000040_3379(BA000040|pid:none) Bradyrhizobium japonicum USDA 1...    70   1e-10
CU234118_4532(CU234118|pid:none) Bradyrhizobium sp. ORS278,compl...    69   2e-10
CP000494_4531(CP000494|pid:none) Bradyrhizobium sp. BTAi1, compl...    67   6e-10
CP000085_877(CP000085|pid:none) Burkholderia thailandensis E264 ...    64   9e-09
BX571966_1516(BX571966|pid:none) Burkholderia pseudomallei strai...    63   2e-08
BA000012_1330(BA000012|pid:none) Mesorhizobium loti MAFF303099 D...    60   8e-08
CP000283_1918(CP000283|pid:none) Rhodopseudomonas palustris BisB...    52   2e-05
BX640428_188(BX640428|pid:none) Bordetella parapertussis strain ...    50   1e-04
BX640414_202(BX640414|pid:none) Bordetella pertussis strain Toha...    50   1e-04
protein update 2009. 3.24
PSORT 

 psg: 0.71  gvh: 0.52  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 28.0 %: cytoplasmic
	 24.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for VSH527 is nuc
5' end seq. ID VSH527F
5' end seq. 
>VSH527F.Seq
ATGTCAACAAGAGTACAAAAATCTGCAATTTTAAATGAAAACATAGACAAAGTATGGAAT
GTTTTACGTAATTTCGATTTCCCATCAAAAATCTTCCCAGTAATCGAAAGTTCAATTATT
GAAGGTGACTCAACACCAACCACTGTTGGAGCCATCCGTGTTTTAAAGTGGAAAACTGGT
GAAACCAATAAACAAAGATTATTAGAACTCTCTGATCTTTCACATAAAATCATTTATGAA
TTAATTGAAAGTGAACAAACTGCTGAAGTTACTGCCTACATTAGCACCATTAAATTAATT
CGTATTACTGAATCAAATCAAACCCTTTTGACTTGGGAAAGTGAATTTTCAGCAGATGTA
AAGAAGGATTTAATTAATTTCGAATCAAAATCAGTTTCCTTAAATTTACAAGATTTACAA
AAATTCTTTTCAAAATAAATTAAATTCTCTATTCTTTTAATTTTAAAAATTAANAANNAA
A----------
Length of 5' end seq. 481
3' end seq. ID VSH527Z
3' end seq. 
>VSH527Z.Seq
----------ATGTCAACAAGAGTACAAAAATCTGCAATTTTAAATGAAAACATAGACAA
AGTATGGAATGTTTTACGTAATTTCGATTTCCCATCAAAAATCTTCCCAGTAATCGAAAG
TTCAATTATTGAAGGTGACTCAACACCAACCACTGTTGGAGCCATCCGTGTTTTAAAGTG
GAAAACTGGTGAAACCAATAAACAAAGATTATTAGAACTCTCTGATCTTTCACATAAAAT
CATTTATGAATTAATTGAAAGTGAACAAACTGCTGAAGTTACTGCCTACATTAGCACCAT
TAAATTAATTCGTATTACTGAATCAAATCAAACCCTTTTGACTTGGGAAAGTGAATTTTC
AGCAGATGTAAAGAAGGATTTAATTAATTTCGAATCAAAATCAGTTTCCTTAAATTTACA
AGATTTACAAAAATTCTTTTCAAAATAAATTAAATTACTCTATTCTTTTAATTTTAAAAA
Length of 3' end seq. 470
Connected seq. ID VSH527P
Connected seq. 
>VSH527P.Seq
ATGTCAACAAGAGTACAAAAATCTGCAATTTTAAATGAAAACATAGACAAAGTATGGAAT
GTTTTACGTAATTTCGATTTCCCATCAAAAATCTTCCCAGTAATCGAAAGTTCAATTATT
GAAGGTGACTCAACACCAACCACTGTTGGAGCCATCCGTGTTTTAAAGTGGAAAACTGGT
GAAACCAATAAACAAAGATTATTAGAACTCTCTGATCTTTCACATAAAATCATTTATGAA
TTAATTGAAAGTGAACAAACTGCTGAAGTTACTGCCTACATTAGCACCATTAAATTAATT
CGTATTACTGAATCAAATCAAACCCTTTTGACTTGGGAAAGTGAATTTTCAGCAGATGTA
AAGAAGGATTTAATTAATTTCGAATCAAAATCAGTTTCCTTAAATTTACAAGATTTACAA
AAATTCTTTTCAAAATAAATTAAATTCTCTATTCTTTTAATTTTAAAAATTAANAANNAA
A----------ATGTCAACAAGAGTACAAAAATCTGCAATTTTAAATGAAAACATAGACA
AAGTATGGAATGTTTTACGTAATTTCGATTTCCCATCAAAAATCTTCCCAGTAATCGAAA
GTTCAATTATTGAAGGTGACTCAACACCAACCACTGTTGGAGCCATCCGTGTTTTAAAGT
GGAAAACTGGTGAAACCAATAAACAAAGATTATTAGAACTCTCTGATCTTTCACATAAAA
TCATTTATGAATTAATTGAAAGTGAACAAACTGCTGAAGTTACTGCCTACATTAGCACCA
TTAAATTAATTCGTATTACTGAATCAAATCAAACCCTTTTGACTTGGGAAAGTGAATTTT
CAGCAGATGTAAAGAAGGATTTAATTAATTTCGAATCAAAATCAGTTTCCTTAAATTTAC
AAGATTTACAAAAATTCTTTTCAAAATAAATTAAATTACTCTATTCTTTTAATTTTAAAA
A
Length of connected seq. 951
Full length Seq ID -
Full length Seq. -
Length of full length seq. -