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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFB838
Library AF
(Link to library)
Clone ID AFB838
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16391-1
Original site URL
Representative seq. ID AFB838P
(Link to Original site)
Representative DNA sequence 
>AFB838 (AFB838Q) /CSM/AF/AFB8-B/AFB838Q.Seq.d/
ATAAACGTACAATTATAATATAAAAACAATCACACATTTTGTAATAGTAGTAACAGTTAT
ATTTAAATATATAGGTAAAAAAACAGTAACATAAAGGAAAAGAAAAACATATAATAGATT
TCCTTTTTTTTTTTTTTTTTNCCCXXXXXXXXXXAATTAAAAATAATGCTCTCATTGGAT
GTAAATCGGCTCTTGGTGGTTCTCCTTTTGCCATCTCTAATGCTGTAATACCCATACTCC
AAATATCAGCTTTTGAATCATATCCAGTTTGTTTAATAACTTCAGGTGCCATCCAAAATG
GGGTACCAACAAATGTATTTCTCTTTGTCATTTGATCAGTTAATTGACCACTAACACCAA
AATCAGCTAATTTAACATCACCGCTTGCTGAAAGTAAAACATTTGCAGCTTTAATATCTC
TGTGAANTTTACCTTCTGAATGTGAATATTC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
krtiii*kqshil****qlylni*vkkq*HKGKEKHIIDFLFFFFFX---

---IKNNALIGCKSALGGSPFAISNAVIPILQISAFESYPVCLITSGAIQNGVPTNVFLF
VI*svn*pltpksanltsplaesktfaalisl*xlpsecey
Translated Amino Acid sequence (All Frames) 
Frame A:
invql*yknnhtfcnssnsyi*iyr*knsnikekkni**isfffffxp---

---n*k*cshwm*igswwfsfchl*ccnthtpnisf*iisslfnnfrchpkwgtnkcisl
chlis*lttntkis*fnitac*k*nicsfnisvxftf*m*if

Frame B:
*tynyniktithfvivvtvifkyigkktvt*rkrktynrfpfffffx---

---IKNNALIGCKSALGGSPFAISNAVIPILQISAFESYPVCLITSGAIQNGVPTNVFLF
VI*svn*pltpksanltsplaesktfaalisl*xlpsecey

Frame C:
krtiii*kqshil****qlylni*vkkq*HKGKEKHIIDFLFFFFFX---

---lkimlsldvnrllvvlllpslml*ypyskyqllnhiqfv**lqvpskmgyqqmyfsl
sfdqlidh*hqnqli*hhrllkvkhlql*ylcexyllnvni
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFB838 (AFB838Q) /CSM/AF/AFB8-B/AFB838Q.Seq.d/                        583   e-166
SFD665 (SFD665Q) /CSM/SF/SFD6-C/SFD665Q.Seq.d/                        575   e-163
CFK466 (CFK466Q) /CSM/CF/CFK4-C/CFK466Q.Seq.d/                        575   e-163
AFN655 (AFN655Q) /CSM/AF/AFN6-C/AFN655Q.Seq.d/                        575   e-163
AFN101 (AFN101Q) /CSM/AF/AFN1-A/AFN101Q.Seq.d/                        573   e-163
AFI880 (AFI880Q) /CSM/AF/AFI8-D/AFI880Q.Seq.d/                        573   e-163
SFH469 (SFH469Q) /CSM/SF/SFH4-C/SFH469Q.Seq.d/                        569   e-161
VFH696 (VFH696Q) /CSM/VF/VFH6-D/VFH696Q.Seq.d/                        567   e-161
SLG682 (SLG682Q) /CSM/SL/SLG6-D/SLG682Q.Seq.d/                        567   e-161
SFD620 (SFD620Q) /CSM/SF/SFD6-A/SFD620Q.Seq.d/                        561   e-159
own update 2009. 4. 3
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF059534|AF059534.1 Dictyostelium discoideum severin kinase mRNA, complete cds.   575  e-160  1
AF061281|AF061281.1 Dictyostelium discoideum kinase responsive to stress 1-like kinase mRNA, complete cds.    66  7e-19  4
AL872036|AL872036.1 Xenopus tropicalis EST, clone TEgg111e07 5'.    46  4e-13  4
BD126332|BD126332.1 Primer for synthesizing full-length cDNA and use thereof.    58  2e-12  4
BD124923|BD124923.1 Primer for synthesizing full-length cDNA and use thereof.    58  2e-12  4
BX369471|BX369471.1 human full-length cDNA 5-PRIME end of clone CS0DJ014YC06 of T CELLS  (JURKAT CELL LINE) COT 10-NORMALIZED of Homo sapiens (human).    58  4e-12  4
BJ073918|BJ073918.1 Xenopus laevis cDNA clone:XL110a13, 5' end, single read.    42  4e-12  4
BD127314|BD127314.1 Primer for synthesizing full-length cDNA and use thereof.    58  5e-12  5
AK074837|AK074837.1 Homo sapiens cDNA FLJ90356 fis, clone NT2RP2003545, weakly similar to Homo sapiens STE20-like kinase 3 (mst-3) mRNA.    58  5e-12  5
AK000556|AK000556.1 Homo sapiens cDNA FLJ20549 fis, clone KAT11561.    58  6e-12  5
dna update 2003. 7.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(O61122) RecName: Full=Serine/threonine-protein kinase svkA;    ...   198   4e-50
AE017347_297(AE017347|pid:none) Cryptococcus neoformans var. neo...   171   9e-42
BC045867_1(BC045867|pid:none) Danio rerio serine/threonine kinas...   170   1e-41
BT045853_1(BT045853|pid:none) Salmo salar clone ssal-rgf-534-302...   170   1e-41
BX897733_7(BX897733|pid:none) Zebrafish DNA sequence from clone ...   170   1e-41
BC054651_1(BC054651|pid:none) Danio rerio serine/threonine kinas...   170   2e-41
BC167945_1(BC167945|pid:none) Xenopus tropicalis cDNA clone MGC:...   170   2e-41
AK291808_1(AK291808|pid:none) Homo sapiens cDNA FLJ78718 complet...   169   3e-41
(Q99JT2) RecName: Full=Serine/threonine-protein kinase MST4;    ...   169   3e-41
AK303719_1(AK303719|pid:none) Homo sapiens cDNA FLJ61527 complet...   169   3e-41
protein update 2009. 3.27
PSORT 

 psg: 1.01  gvh: 0.46  alm: 0.36  top: 0.30  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFB838 is cyt
5' end seq. ID AFB838F
5' end seq. 
>AFB838F.Seq
ATAAACGTACAATTATAATATAAAAACAATCACACATTTTGTAATAGTAGTAACAGTTAT
ATTTAAATATATAGGTAAAAAAACAGTAACATAAAGGAAAAGAAAAACATATAATAGATT
TCCTTTTTTTTTTTTTTTTTNCCC----------
Length of 5' end seq. 144
3' end seq. ID AFB838Z
3' end seq. 
>AFB838Z.Seq
----------AATTAAAAATAATGCTCTCATTGGATGTAAATCGGCTCTTGGTGGTTCTC
CTTTTGCCATCTCTAATGCTGTAATACCCATACTCCAAATATCAGCTTTTGAATCATATC
CAGTTTGTTTAATAACTTCAGGTGCCATCCAAAATGGGGTACCAACAAATGTATTTCTCT
TTGTCATTTGATCAGTTAATTGACCACTAACACCAAAATCAGCTAATTTAACATCACCGC
TTGCTGAAAGTAAAACATTTGCAGCTTTAATATCTCTGTGAANTTTACCTTCTGAATGTG
AATATTC
Length of 3' end seq. 297
Connected seq. ID AFB838P
Connected seq. 
>AFB838P.Seq
ATAAACGTACAATTATAATATAAAAACAATCACACATTTTGTAATAGTAGTAACAGTTAT
ATTTAAATATATAGGTAAAAAAACAGTAACATAAAGGAAAAGAAAAACATATAATAGATT
TCCTTTTTTTTTTTTTTTTTNCCC----------AATTAAAAATAATGCTCTCATTGGAT
GTAAATCGGCTCTTGGTGGTTCTCCTTTTGCCATCTCTAATGCTGTAATACCCATACTCC
AAATATCAGCTTTTGAATCATATCCAGTTTGTTTAATAACTTCAGGTGCCATCCAAAATG
GGGTACCAACAAATGTATTTCTCTTTGTCATTTGATCAGTTAATTGACCACTAACACCAA
AATCAGCTAATTTAACATCACCGCTTGCTGAAAGTAAAACATTTGCAGCTTTAATATCTC
TGTGAANTTTACCTTCTGAATGTGAATATTC
Length of connected seq. 441
Full length Seq ID -
Full length Seq. -
Length of full length seq. -