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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSH274
Library VS
(Link to library)
Clone ID VSH274
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14663-1
Original site URL
Representative seq. ID VSH274P
(Link to Original site)
Representative DNA sequence 
>VSH274 (VSH274Q) /CSM/VS/VSH2-D/VSH274Q.Seq.d/
GAACACACACACATAACCAATCATACTATTGTTATAATATCTTTTTTTTTTTTTTTXXXX
XXXXXXTTTAGTTTTAGTTATTGTTGGTGGTATTTGTGTTAGCTTTTTATCAAATTTCAA
AAGATTTCACAAAAGCCAGTGATGCTAAATCAGCTCTTTCAAATCTTACAACCAGTATTC
CAGCTGGTGTTTTAGTTATTGGTATCCTCTTTGTAATTTTCACTGTTGTTGGTTGTTTCG
TTGCTTACAAAGAGAAATTAGTTGGTTTAGTAATCTATTGTGCAGTAATGTTAATTTTAT
TAGTTATTTTAATTGGTGTTGGTGGTAAAGCTATAACATTACACAATGATGATATCATTA
ATGAAGTTGGTGGTGCTTGGGAACATGTTGCCAATGGTACAAAGAATAGCACCCTCACTC
GTCTCGAAAATTTCTTAAAATGTTGTAAATGGTCAAACGTCTCAATAGACTCTTCAGATT
TATGTCCAAAAGATGGTGATAAAATTAAATATGAAGGTCACTATTGTGGTGAAGCTTTAA
GTGACCAATTCAGTTCAAAAATCTATGCTGTTGGTGCTGCTGGTTTAGCCATTGGTATCA
TTGAACTCGTTGCCATCCTTTTCTCATTATTCTTAATTATTCGTATTTGTCGTTCACCAA
GAACTCGTTCATANGCATCAATATTAAATAAATAAATAAAACAAAATAAATAAATAATAG
TAATAGAATCAAAAATTT
sequence update 2001. 3.22
Translated Amino Acid sequence 
EHTHITNHTIVIISFFFF---

---LVLVIVGGICVSFLSNFKRFHKSQ*c*issfksynqysswcfsywyplcnfhccwlf
rclqreiswfsnllcsnvnfisyfnwcww*synitq**yh**swwclgtccqwyke*hph
ssrkflkml*mvkrlnrlfrfmskrw**n*i*rsllw*sfk*piqfknlccwccwfshwy
h*trchpfliilnysylsftknsfixinik*inktk*innsnrikn
Translated Amino Acid sequence (All Frames) 
Frame A:
EHTHITNHTIVIISFFFF---

---fsfsycwwylc*lfikfqkisqkpvmlnqlfqilqpvfqlvf*llvssl*fslllvv
slltkrn*lv**sivq*c*fy*lf*lvlvvkl*hytmmislmklvvlgnmlpmvqriaps
lvskis*nvvngqtsq*tlqiyvqkmviklnmkvtivvkl*vtnsvqksmllvllv*plv
slnslpsfshys*lfvfvvhqelvhxhqy*ink*nkink****nqkf

Frame B:
ntht*piilll*ylffff---

---LVLVIVGGICVSFLSNFKRFHKSQ*c*issfksynqysswcfsywyplcnfhccwlf
rclqreiswfsnllcsnvnfisyfnwcww*synitq**yh**swwclgtccqwyke*hph
ssrkflkml*mvkrlnrlfrfmskrw**n*i*rsllw*sfk*piqfknlccwccwfshwy
h*trchpfliilnysylsftknsfixinik*inktk*innsnrikn

Frame C:
ththnqsyycynifffff---

---*f*lllvvfvlafyqiskdftkasdaksalsnlttsipagvlvigilfviftvvgcf
vaykeklvglviycavmlillviligvggkaitlhnddiinevggawehvangtknstlt
rlenflkcckwsnvsidssdlcpkdgdkikyeghycgealsdqfsskiyavgaaglaigi
ielvailfslfliiricrsprtrsxasilnk*ikqnk*iivieski
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSH274 (VSH274Q) /CSM/VS/VSH2-D/VSH274Q.Seq.d/                       1207   0.0
VSD501 (VSD501Q) /CSM/VS/VSD5-A/VSD501Q.Seq.d/                       1187   0.0
SSE714 (SSE714Q) /CSM/SS/SSE7-A/SSE714Q.Seq.d/                       1174   0.0
VFJ357 (VFJ357Q) /CSM/VF/VFJ3-C/VFJ357Q.Seq.d/                       1134   0.0
SHL205 (SHL205Q) /CSM/SH/SHL2-A/SHL205Q.Seq.d/                       1124   0.0
SLF483 (SLF483Q) /CSM/SL/SLF4-D/SLF483Q.Seq.d/                        961   0.0
SSG881 (SSG881Q) /CSM/SS/SSG8-D/SSG881Q.Seq.d/                        749   0.0
VSD764 (VSD764Q) /CSM/VS/VSD7-C/VSD764Q.Seq.d/                        658   0.0
SSI394 (SSI394Q) /CSM/SS/SSI3-D/SSI394Q.Seq.d/                        656   0.0
SSG410 (SSG410Q) /CSM/SS/SSG4-A/SSG410Q.Seq.d/                        640   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU267180) Dictyostelium discoideum vegetative cDNA clone:VS...  1209   0.0    1
(AB015186) Dictyostelium discoideum mRNA for CD9, complete cds.  1098   0.0    2
(AU264137) Dictyostelium discoideum vegetative cDNA clone:VS...  1098   0.0    2
(C84714) Dictyostelium discoideum slug cDNA, clone SSE714.       1098   0.0    2
(BJ408522) Dictyostelium discoideum cDNA clone:dds46i02, 3' ...  1035   0.0    2
(BJ432706) Dictyostelium discoideum cDNA clone:ddv19b15, 3' ...   989   0.0    3
(AU052998) Dictyostelium discoideum slug cDNA, clone SLF483.      961   0.0    1
(AU264541) Dictyostelium discoideum vegetative cDNA clone:VS...   658   0.0    2
(C90221) Dictyostelium discoideum slug cDNA, clone SSI394.        656   0.0    2
(C94101) Dictyostelium discoideum slug cDNA, clone SSG410.        640   e-179  1
dna update 2009. 6. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55CW7) RecName: Full=Probable tetraspanin tspB;                     187   3e-46
(Q55CV7) RecName: Full=Probable tetraspanin tspC;                     173   4e-42
AE015928_2029(AE015928|pid:none) Bacteroides thetaiotaomicron VP...    39   0.12
(Q5A861) RecName: Full=Palmitoyltransferase SWF1;          EC=2....    37   0.60
CP000500_395(CP000500|pid:none) Pichia stipitis CBS 6054 chromos...    37   0.78
(Q8C110) RecName: Full=SLIT and NTRK-like protein 6; Flags: Prec...    36   1.3
EF025354_1(EF025354|pid:none) Synthetic construct multivalent an...    35   2.3
(P27591) RecName: Full=23 kDa integral membrane protein; AltName...    35   2.3
EF025352_1(EF025352|pid:none) Synthetic construct multivalent an...    35   2.3
EF025355_1(EF025355|pid:none) Synthetic construct multivalent an...    35   2.3
protein update 2009. 3.23
PSORT 

 psg: 0.75  gvh: 0.57  alm: 0.12  top: 0.67  tms: 0.07  mit: 0.35  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: nuclear
	 24.0 %: mitochondrial
	 24.0 %: cytoplasmic
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for VSH274 is nuc
5' end seq. ID VSH274F
5' end seq. 
>VSH274F.Seq
GAACACACACACATAACCAATCATACTATTGTTATAATATCTTTTTTTTTTTTTTT----
------
Length of 5' end seq. 56
3' end seq. ID VSH274Z
3' end seq. 
>VSH274Z.Seq
----------TTTAGTTTTAGTTATTGTTGGTGGTATTTGTGTTAGCTTTTTATCAAATT
TCAAAAGATTTCACAAAAGCCAGTGATGCTAAATCAGCTCTTTCAAATCTTACAACCAGT
ATTCCAGCTGGTGTTTTAGTTATTGGTATCCTCTTTGTAATTTTCACTGTTGTTGGTTGT
TTCGTTGCTTACAAAGAGAAATTAGTTGGTTTAGTAATCTATTGTGCAGTAATGTTAATT
TTATTAGTTATTTTAATTGGTGTTGGTGGTAAAGCTATAACATTACACAATGATGATATC
ATTAATGAAGTTGGTGGTGCTTGGGAACATGTTGCCAATGGTACAAAGAATAGCACCCTC
ACTCGTCTCGAAAATTTCTTAAAATGTTGTAAATGGTCAAACGTCTCAATAGACTCTTCA
GATTTATGTCCAAAAGATGGTGATAAAATTAAATATGAAGGTCACTATTGTGGTGAAGCT
TTAAGTGACCAATTCAGTTCAAAAATCTATGCTGTTGGTGCTGCTGGTTTAGCCATTGGT
ATCATTGAACTCGTTGCCATCCTTTTCTCATTATTCTTAATTATTCGTATTTGTCGTTCA
CCAAGAACTCGTTCATANGCATCAATATTAAATAAATAAATAAAACAAAATAAATAAATA
ATAGTAATAGAATCAAAAATTT
Length of 3' end seq. 672
Connected seq. ID VSH274P
Connected seq. 
>VSH274P.Seq
GAACACACACACATAACCAATCATACTATTGTTATAATATCTTTTTTTTTTTTTTT----
------TTTAGTTTTAGTTATTGTTGGTGGTATTTGTGTTAGCTTTTTATCAAATTTCAA
AAGATTTCACAAAAGCCAGTGATGCTAAATCAGCTCTTTCAAATCTTACAACCAGTATTC
CAGCTGGTGTTTTAGTTATTGGTATCCTCTTTGTAATTTTCACTGTTGTTGGTTGTTTCG
TTGCTTACAAAGAGAAATTAGTTGGTTTAGTAATCTATTGTGCAGTAATGTTAATTTTAT
TAGTTATTTTAATTGGTGTTGGTGGTAAAGCTATAACATTACACAATGATGATATCATTA
ATGAAGTTGGTGGTGCTTGGGAACATGTTGCCAATGGTACAAAGAATAGCACCCTCACTC
GTCTCGAAAATTTCTTAAAATGTTGTAAATGGTCAAACGTCTCAATAGACTCTTCAGATT
TATGTCCAAAAGATGGTGATAAAATTAAATATGAAGGTCACTATTGTGGTGAAGCTTTAA
GTGACCAATTCAGTTCAAAAATCTATGCTGTTGGTGCTGCTGGTTTAGCCATTGGTATCA
TTGAACTCGTTGCCATCCTTTTCTCATTATTCTTAATTATTCGTATTTGTCGTTCACCAA
GAACTCGTTCATANGCATCAATATTAAATAAATAAATAAAACAAAATAAATAAATAATAG
TAATAGAATCAAAAATTT
Length of connected seq. 728
Full length Seq ID -
Full length Seq. -
Length of full length seq. -