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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSH265
Library VS
(Link to library)
Clone ID VSH265
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12167-1
Original site URL
Representative seq. ID VSH265P
(Link to Original site)
Representative DNA sequence 
>VSH265 (VSH265Q) /CSM/VS/VSH2-C/VSH265Q.Seq.d/
ATGATCAATGGGTAACTTCATACAAATTAAGATACACACATAGCATATAAATTAACAACA
ATGGAAGGAGAACAATTACAAACTGCACCATACAACCCAAGATTCCCACAACAAAACCAA
ACTAAACATTGTTGGGCTAACTACGTTGATTACTATGGTTGTGTCAAACATTACAATGGT
GACAACTCAAAATGTCAAACTTTTTTCAACTCAATGAACTCATTATGTCCAGCTGCCTGG
ATTTCAGAATGGGATGAACAAAAAGCCGCTGATCTCTTCCCATCAGACAGAGTTTAAATA
TATAATATAAATAATTAATAGTAGTATCAATAATTTATTCAAAAAAAAAAXXXXXXXXXX
ATGATCAATGGGTAACTTCATACAAATTAAGATACACACATAGCATATAAATTAACAACA
ATGGAAGGAGAACAATTACAAACTGCACCATACAACCCAAGATTCCCACAACAAAACCAA
ACTAAACATTGTTGGGCTAACTACGTTGATTACTATGGTTGTGTCAAACATTACAATGGT
GACAACTCAAAATGTCAAACTTTTTTCAACTCAATGAACTCATTATGTCCAGCTGCCTGG
ATTTCAGAATGGGATGAACAAAAAGCCGCTNATCTCTTCCCATCAGACAGAGTTTAAATA
TATAATATAAATAATTAATAGAAGTATCAATAATT
sequence update 2001. 3.22
Translated Amino Acid sequence 
*smgnfiqikiht*hin*qqwkennyklhhttqdshnktklnivglttlitmvvsnitmv
ttqnvklfstq*thyvqlpgfqngmnkkplisshqtefkyii*IINSSINNLFKKK---

---DQWVTSYKLRYTHSI*innngrrtitnctiqpkipttkpn*tllg*lr*llwlcqtl
qw*qlkmsnffqlnelimsscldfrmg*tksrxslpirqslni*yk*lievsii
Translated Amino Acid sequence (All Frames) 
Frame A:
ming*lhtn*dthiayklttmegeqlqtapynprfpqqnqtkhcwanyvdyygcvkhyng
dnskcqtffnsmnslcpaawisewdeqkaadlfpsdrv*iyninn***yq*fiqkk---

---ming*lhtn*dthiayklttmegeqlqtapynprfpqqnqtkhcwanyvdyygcvkh
yngdnskcqtffnsmnslcpaawisewdeqkaaxlfpsdrv*iyninn**kyq*

Frame B:
*smgnfiqikiht*hin*qqwkennyklhhttqdshnktklnivglttlitmvvsnitmv
ttqnvklfstq*thyvqlpgfqngmnkkplisshqtefkyii*IINSSINNLFKKK---

---*smgnfiqikiht*hin*qqwkennyklhhttqdshnktklnivglttlitmvvsni
tmvttqnvklfstq*thyvqlpgfqngmnkkplisshqtefkyii*iinrsinn

Frame C:
dqwvtsyklrythsi*innngrrtitnctiqpkipttkpn*tllg*lr*llwlcqtlqw*
qlkmsnffqlnelimsscldfrmg*tksr*slpirqslni*yk*livvsiiyskkk---

---DQWVTSYKLRYTHSI*innngrrtitnctiqpkipttkpn*tllg*lr*llwlcqtl
qw*qlkmsnffqlnelimsscldfrmg*tksrxslpirqslni*yk*lievsii
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSH265 (VSH265Q) /CSM/VS/VSH2-C/VSH265Q.Seq.d/                        674   0.0
VSD559 (VSD559Q) /CSM/VS/VSD5-C/VSD559Q.Seq.d/                        613   e-174
VHK558 (VHK558Q) /CSM/VH/VHK5-C/VHK558Q.Seq.d/                        613   e-174
VSA196 (VSA196Q) /CSM/VS/VSA1-D/VSA196Q.Seq.d/                        605   e-172
VSI263 (VSI263Q) /CSM/VS/VSI2-C/VSI263Q.Seq.d/                        603   e-171
SSF773 (SSF773Q) /CSM/SS/SSF7-D/SSF773Q.Seq.d/                        589   e-167
VSH131 (VSH131Q) /CSM/VS/VSH1-B/VSH131Q.Seq.d/                        587   e-167
SSE738 (SSE738Q) /CSM/SS/SSE7-B/SSE738Q.Seq.d/                        583   e-165
VSJ228 (VSJ228Q) /CSM/VS/VSJ2-B/VSJ228Q.Seq.d/                        565   e-160
VSF762 (VSF762Q) /CSM/VS/VSF7-C/VSF762Q.Seq.d/                        565   e-160
own update 2003. 1.10
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU267162) Dictyostelium discoideum vegetative cDNA clone:VS...   674   0.0    1
(AU267163) Dictyostelium discoideum vegetative cDNA clone:VS...   658   0.0    1
(AU264240) Dictyostelium discoideum vegetative cDNA clone:VS...   613   e-171  1
(BJ424463) Dictyostelium discoideum cDNA clone:ddv52c15, 5' ...   613   e-171  1
(AU261346) Dictyostelium discoideum vegetative cDNA clone:VS...   605   e-169  1
(AU268413) Dictyostelium discoideum vegetative cDNA clone:VS...   603   e-168  1
(C93078) Dictyostelium discoideum slug cDNA, clone SSF773.        589   e-164  1
(C84718) Dictyostelium discoideum slug cDNA, clone SSE738.        583   e-162  1
(AU266949) Dictyostelium discoideum vegetative cDNA clone:VS...   474   e-161  2
(AU269606) Dictyostelium discoideum vegetative cDNA clone:VS...   565   e-157  1
dna update 2009. 6. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q01519) RecName: Full=Cytochrome c oxidase polypeptide VIb;    ...    89   1e-16
AM920427_744(AM920427|pid:none) Penicillium chrysogenum Wisconsi...    87   3e-16
AM269997_1(AM269997|pid:none) Aspergillus niger contig An02c0020...    87   6e-16
CR382131_683(CR382131|pid:none) Yarrowia lipolytica strain CLIB1...    86   1e-15
CR382126_746(CR382126|pid:none) Kluyveromyces lactis strain NRRL...    86   1e-15
CP000497_298(CP000497|pid:none) Pichia stipitis CBS 6054 chromos...    84   5e-15
AE017346_138(AE017346|pid:none) Cryptococcus neoformans var. neo...    84   5e-15
(P14854) RecName: Full=Cytochrome c oxidase subunit VIb isoform ...    82   1e-14
(Q5RCT0) RecName: Full=Cytochrome c oxidase subunit VIb isoform ...    82   1e-14
AE014298_3053(AE014298|pid:none) Drosophila melanogaster chromos...    80   4e-14
protein update 2009. 3.23
PSORT 

 psg: 0.50  gvh: 0.07  alm: 0.67  top: 0.53  tms: 0.00  mit: 0.42  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 24.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for VSH265 is nuc
5' end seq. ID VSH265F
5' end seq. 
>VSH265F.Seq
ATGATCAATGGGTAACTTCATACAAATTAAGATACACACATAGCATATAAATTAACAACA
ATGGAAGGAGAACAATTACAAACTGCACCATACAACCCAAGATTCCCACAACAAAACCAA
ACTAAACATTGTTGGGCTAACTACGTTGATTACTATGGTTGTGTCAAACATTACAATGGT
GACAACTCAAAATGTCAAACTTTTTTCAACTCAATGAACTCATTATGTCCAGCTGCCTGG
ATTTCAGAATGGGATGAACAAAAAGCCGCTGATCTCTTCCCATCAGACAGAGTTTAAATA
TATAATATAAATAATTAATAGTAGTATCAATAATTTATTCAAAAAAAAAA----------
Length of 5' end seq. 350
3' end seq. ID VSH265Z
3' end seq. 
>VSH265Z.Seq
----------ATGATCAATGGGTAACTTCATACAAATTAAGATACACACATAGCATATAA
ATTAACAACAATGGAAGGAGAACAATTACAAACTGCACCATACAACCCAAGATTCCCACA
ACAAAACCAAACTAAACATTGTTGGGCTAACTACGTTGATTACTATGGTTGTGTCAAACA
TTACAATGGTGACAACTCAAAATGTCAAACTTTTTTCAACTCAATGAACTCATTATGTCC
AGCTGCCTGGATTTCAGAATGGGATGAACAAAAAGCCGCTNATCTCTTCCCATCAGACAG
AGTTTAAATATATAATATAAATAATTAATAGAAGTATCAATAATT
Length of 3' end seq. 335
Connected seq. ID VSH265P
Connected seq. 
>VSH265P.Seq
ATGATCAATGGGTAACTTCATACAAATTAAGATACACACATAGCATATAAATTAACAACA
ATGGAAGGAGAACAATTACAAACTGCACCATACAACCCAAGATTCCCACAACAAAACCAA
ACTAAACATTGTTGGGCTAACTACGTTGATTACTATGGTTGTGTCAAACATTACAATGGT
GACAACTCAAAATGTCAAACTTTTTTCAACTCAATGAACTCATTATGTCCAGCTGCCTGG
ATTTCAGAATGGGATGAACAAAAAGCCGCTGATCTCTTCCCATCAGACAGAGTTTAAATA
TATAATATAAATAATTAATAGTAGTATCAATAATTTATTCAAAAAAAAAA----------
ATGATCAATGGGTAACTTCATACAAATTAAGATACACACATAGCATATAAATTAACAACA
ATGGAAGGAGAACAATTACAAACTGCACCATACAACCCAAGATTCCCACAACAAAACCAA
ACTAAACATTGTTGGGCTAACTACGTTGATTACTATGGTTGTGTCAAACATTACAATGGT
GACAACTCAAAATGTCAAACTTTTTTCAACTCAATGAACTCATTATGTCCAGCTGCCTGG
ATTTCAGAATGGGATGAACAAAAAGCCGCTNATCTCTTCCCATCAGACAGAGTTTAAATA
TATAATATAAATAATTAATAGAAGTATCAATAATT
Length of connected seq. 685
Full length Seq ID -
Full length Seq. -
Length of full length seq. -