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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSH234
Library VS
(Link to library)
Clone ID VSH234
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U06849-1
Original site URL
Representative seq. ID VSH234P
(Link to Original site)
Representative DNA sequence 
>VSH234 (VSH234Q) /CSM/VS/VSH2-B/VSH234Q.Seq.d/
CTCTTATATATGCAATCTTTAATTGACTCAAAGGTTGATTTCATACCTAAATTTGATAAT
TTAATGTTATTTAATGATGCTGATGCTGATAATGATAATTATAATAATAATAATAATAAT
AATAAAACAAAAACAACCAATAACGATAACACAATAAACACTTTAGAAATTGAAAAATTA
TTATCATCATTCACTTTATCTAAAAGATTAAATATAGATGATCATTATCTTAGTGGAGTT
AAATTACCAAACCCAAGTAATATTGTACATTTAAAAAGAAAATCAAATAAACATTTAGTA
TCAGCAGAATCTGTAAATAAACAACAACAACAACAACAACCACAACCACAACATGAACAA
ATTAAACAACAAAAAACAATTTCAAATACTCAAACCAATACCATCATTAATAAAGATAAC
GAAGAGATTACATTATCACCAATAGTAAAAGATACAGCAGATAAAGCACCAATTGATAAT
GGAAATGTTACAAGTGATAATTTXXXXXXXXXXCAAAGGTTGATTTNATANTTAAATTTG
ATAATTTAATGTTATTTAATNATGCTGATGC
sequence update 2001. 3.22
Translated Amino Acid sequence 
LLYMQSLIDSKVDFIPKFDNLMLFNDADADNDNYNNNNNNNKTKTTNNDNTINTLEIEKL
LSSFTLSKRLNIDDHYLSGVKLPNPSNIVHLKRKSNKHLVSAESVNKQQQQQQPQPQHEQ
IKQQKTISNTQTNTIINKDNEEITLSPIVKDTADKAPIDNGNVTSDN---

---KVDXIXKFDNLMLFNXAD
Translated Amino Acid sequence (All Frames) 
Frame A:
LLYMQSLIDSKVDFIPKFDNLMLFNDADADNDNYNNNNNNNKTKTTNNDNTINTLEIEKL
LSSFTLSKRLNIDDHYLSGVKLPNPSNIVHLKRKSNKHLVSAESVNKQQQQQQPQPQHEQ
IKQQKTISNTQTNTIINKDNEEITLSPIVKDTADKAPIDNGNVTSDN---

---qrlixxlnlii*cylxmlm

Frame B:
syicnl*ltqrlisylnlii*cylmmlmlimiiiiiiiiiikqkqpititq*tl*klkny
yhhslylkd*i*miiilvelnyqtqvilyi*kenqini*yqqnl*innnnnnnhnhnmnk
lnnkkqfqilkpipslikitkrlhyhq**kiqqikhqlimemlqvii---

---kg*fxx*i**fnvi*xc*c

Frame C:
liyaifn*lkg*fht*i**fnvi**c*c****l********nknnq*r*hnkhfrn*kii
iiihfi*kikyr*sls*ws*itkpk*yctfkkkik*tfsisrick*ttttttttttt*tn
*ttknnfkysnqyhh**r*rrdyiitnskrysr*stn**wkcyk**f---

---KVDXIXKFDNLMLFNXAD
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSH234 (VSH234Q) /CSM/VS/VSH2-B/VSH234Q.Seq.d/                        383   e-105
SLD172 (SLD172Q) /CSM/SL/SLD1-C/SLD172Q.Seq.d/                         40   0.036
SFE362 (SFE362Q) /CSM/SF/SFE3-C/SFE362Q.Seq.d/                         40   0.036
CHD384 (CHD384Q) /CSM/CH/CHD3-D/CHD384Q.Seq.d/                         40   0.036
CFJ618 (CFJ618Q) /CSM/CF/CFJ6-A/CFJ618Q.Seq.d/                         40   0.036
CFJ518 (CFJ518Q) /CSM/CF/CFJ5-A/CFJ518Q.Seq.d/                         40   0.036
CFH863 (CFH863Q) /CSM/CF/CFH8-C/CFH863Q.Seq.d/                         40   0.036
CFC505 (CFC505Q) /CSM/CF/CFC5-A/CFC505Q.Seq.d/                         40   0.036
VHI889 (VHI889Q) /CSM/VH/VHI8-D/VHI889Q.Seq.d/                         38   0.14
VHG477 (VHG477Q) /CSM/VH/VHG4-D/VHG477Q.Seq.d/                         38   0.14
own update 2003. 1.10
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU267113) Dictyostelium discoideum vegetative cDNA clone:VS...   278   e-146  3
(U66909) Dictyostelium discoideum ORF DG1003 gene, complete ...   206   e-135  4
(AU267114) Dictyostelium discoideum vegetative cDNA clone:VS...    98   4e-16  1
(AP006628) Onion yellows phytoplasma OY-M DNA, complete genome.    44   0.12   8
(AC103223) Rattus norvegicus clone CH230-50N22, *** SEQUENCI...    34   0.21   2
(AC107538) Rattus norvegicus clone CH230-213H15, *** SEQUENC...    34   0.21   2
(FH337915) CHO_OF4598xn21r1.ab1 CHO_OF4 Nicotiana tabacum ge...    38   0.27   2
(FH255395) CHO_OF3009xk03r1.ab1 CHO_OF3 Nicotiana tabacum ge...    38   0.29   2
(DH174973) Oryzias latipes Fosmid clone:GOLWFno452_d15, reve...    48   0.37   1
(EC313530) REPROTRACTF081141F16 POSSUM_01-POSSUM-C-REPROTRAC...    48   0.37   1
dna update 2009. 6. 6
Homology vs Protein 

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3019103
Number of Hits to DB: 455,656,292
protein update 2009. 3.23
PSORT 

 psg: 0.61  gvh: 0.43  alm: 0.53  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system

>> prediction for VSH234 is cyt
5' end seq. ID VSH234F
5' end seq. 
>VSH234F.Seq
CTCTTATATATGCAATCTTTAATTGACTCAAAGGTTGATTTCATACCTAAATTTGATAAT
TTAATGTTATTTAATGATGCTGATGCTGATAATGATAATTATAATAATAATAATAATAAT
AATAAAACAAAAACAACCAATAACGATAACACAATAAACACTTTAGAAATTGAAAAATTA
TTATCATCATTCACTTTATCTAAAAGATTAAATATAGATGATCATTATCTTAGTGGAGTT
AAATTACCAAACCCAAGTAATATTGTACATTTAAAAAGAAAATCAAATAAACATTTAGTA
TCAGCAGAATCTGTAAATAAACAACAACAACAACAACAACCACAACCACAACATGAACAA
ATTAAACAACAAAAAACAATTTCAAATACTCAAACCAATACCATCATTAATAAAGATAAC
GAAGAGATTACATTATCACCAATAGTAAAAGATACAGCAGATAAAGCACCAATTGATAAT
GGAAATGTTACAAGTGATAATTT----------
Length of 5' end seq. 503
3' end seq. ID VSH234Z
3' end seq. 
>VSH234Z.Seq
----------CAAAGGTTGATTTNATANTTAAATTTGATAATTTAATGTTATTTAATNAT
GCTGATGC
Length of 3' end seq. 58
Connected seq. ID VSH234P
Connected seq. 
>VSH234P.Seq
CTCTTATATATGCAATCTTTAATTGACTCAAAGGTTGATTTCATACCTAAATTTGATAAT
TTAATGTTATTTAATGATGCTGATGCTGATAATGATAATTATAATAATAATAATAATAAT
AATAAAACAAAAACAACCAATAACGATAACACAATAAACACTTTAGAAATTGAAAAATTA
TTATCATCATTCACTTTATCTAAAAGATTAAATATAGATGATCATTATCTTAGTGGAGTT
AAATTACCAAACCCAAGTAATATTGTACATTTAAAAAGAAAATCAAATAAACATTTAGTA
TCAGCAGAATCTGTAAATAAACAACAACAACAACAACAACCACAACCACAACATGAACAA
ATTAAACAACAAAAAACAATTTCAAATACTCAAACCAATACCATCATTAATAAAGATAAC
GAAGAGATTACATTATCACCAATAGTAAAAGATACAGCAGATAAAGCACCAATTGATAAT
GGAAATGTTACAAGTGATAATTT----------CAAAGGTTGATTTNATANTTAAATTTG
ATAATTTAATGTTATTTAATNATGCTGATGC
Length of connected seq. 561
Full length Seq ID -
Full length Seq. -
Length of full length seq. -