>VSG809 (VSG809Q) /CSM/VS/VSG8-A/VSG809Q.Seq.d/
GTTCTAGATCGCGAGCGGTCGCCCTTTTTTTTTTTTTTNATTNATTTTTTTNGTTTTTTT
NGTTTTTTTTTTTTNGXXXXXXXXXX

VLDRERSPFFFFXIXFFXFFXFFFX---

Frame A:
VLDRERSPFFFFXIXFFXFFXFFFX---


Frame B:
f*iasgrpffffxxifxvfxvfff---


Frame C:
srsravalffffxxffxffxffff---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSG809 (VSG809Q) /CSM/VS/VSG8-A/VSG809Q.Seq.d/                         48   2e-05
SLF295 (SLF295Q) /CSM/SL/SLF2-D/SLF295Q.Seq.d/                         48   2e-05
SLC254 (SLC254Q) /CSM/SL/SLC2-C/SLC254Q.Seq.d/                         48   2e-05
SLC236 (SLC236Q) /CSM/SL/SLC2-B/SLC236Q.Seq.d/                         48   2e-05
VSF147 (VSF147Q) /CSM/VS/VSF1-B/VSF147Q.Seq.d/                         42   0.001
VSJ833 (VSJ833Q) /CSM/VS/VSJ8-B/VSJ833Q.Seq.d/                         40   0.005
VSJ150 (VSJ150Q) /CSM/VS/VSJ1-C/VSJ150Q.Seq.d/                         40   0.005
SSG388 (SSG388Q) /CSM/SS/SSG3-D/SSG388Q.Seq.d/                         40   0.005
SSC822 (SSC822Q) /CSM/SS/SSC8-A/SSC822Q.Seq.d/                         40   0.005
SLJ842 (SLJ842Q) /CSM/SL/SLJ8-B/SLJ842Q.Seq.d/                         40   0.005

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BF612178|BF612178.1 daa16c02.y1 NICHD_XGC_Lu1 Xenopus laevis cDNA clone IMAGE:4056195 5' similar to SW:COPA_HUMAN P53621 COATOMER ALPHA SUBUNIT ;, mRNA sequence.    48  0.014  1
BM802223|BM802223.1 AGENCOURT_6461308 NIH_MGC_88 Homo sapiens cDNA clone IMAGE:5560056 5', mRNA sequence.    48  0.014  1
BM593534|BM593534.1 17000687443509 A.Gam.ad.cDNA.blood1 Anopheles gambiae cDNA clone 19600449692125 5', mRNA sequence.    48  0.014  1
BQ919667|BQ919667.1 AGENCOURT_8858608 NCI_CGAP_Mam2 Mus musculus cDNA clone IMAGE:6441744 5', mRNA sequence.    48  0.014  1
CD722181|CD722181.1 oj06e07.y1 Human lacrimal gland, unamplified: oj Homo sapiens cDNA clone oj06e07 5', mRNA sequence.    48  0.014  1
BI376999|BI376999.1 BFLG3_000802 Amphioxus 5-6 hrs cDNA library (Name convention: BFLG or MPMGp498) Branchiostoma floridae cDNA clone MPMGp498P1166 5', mRNA sequence.    48  0.014  1
CD723035|CD723035.1 oj17b11.y1 Human lacrimal gland, unamplified: oj Homo sapiens cDNA clone oj17b11 5', mRNA sequence.    48  0.014  1
AJ442121|AJ442121.1 Gallus gallus EST, clone library dkfz426, clone 1a24r1.    48  0.014  1
BM735656|BM735656.1 UMN05H01 Canine Brain cDNA Library Canis familiaris cDNA 5' similar to PIM-1 ASSOCIATED PROTEIN (PAP-1), mRNA sequence.    48  0.014  1
BM597148|BM597148.1 17000687490356 A.Gam.ad.cDNA.blood1 Anopheles gambiae cDNA clone 19600449717891 5', mRNA sequence.    48  0.014  1

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3019103
Number of Hits to DB: 39,848,782

 psg: 0.98  gvh: 0.38  alm: 0.30  top: 0.47  tms: 0.07  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 36.0 %: mitochondrial
	 24.0 %: nuclear
	 12.0 %: cytoplasmic
	 12.0 %: Golgi
	  8.0 %: endoplasmic reticulum
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane

>> prediction for VSG809 is mit

>VSG809F.Seq
GTTCTAGATCGCGAGCGGTCGCCCTTTTTTTTTTTTTTNATTNATTTTTTTNGTTTTTTT
NGTTTTTTTTTTTTNG----------