>VSG453 (VSG453Q) /CSM/VS/VSG4-C/VSG453Q.Seq.d/
ACTTGACAAAATAATAAATGATATTAAAAAAGAGGCTAACGACTCTGGTGTTACTTTAGC
TCCACTTTCTGTTCCAAAACCTAAACTAGAGGAACTCTCAGAGCAACAAAAAATTATACT
TGCCGAATACATTGCAGAAGTTGGTTTACAGAATATAACAGCGATCACTCTCTCAAAAAA
ATTGAATATTACCGTAGAAAAAGCTAAAAATTATATAAAAAATTCTAACAGACTTGGCAG
AACAAATAATTTAAAAACTATTGGTATTCTTCAAGAAGAGGTTTCATCTATGGAGGCTAA
ATCTATGACTTGGTAAAGATTTAGGTATAGAACTTGGTTCATTATTTTTTCTAAAAAAAA
TAAAACACTAAAATTAAAATATGAAAATAAAATAAAATAAAGCTAATCATTTAATAGTGA
TTAACAAACCCTCATTGTACGTAAAAAAAAAAXXXXXXXXXXTTGNCNNNATAATCNATG
ATATTANAAAAGNGGCTAACGACTCTGGTGTTACTTTAGCTCCACTTTCTGTTCCAAAAC
CTAAACTAGAGGAACTCTCAGAGCAACAAAAAATTATACTTGCCGAATACATTGCAGAAG
TTGGTTTACAGAATATAACAGCGATCACTCTCTCAAAAAAATTGAATATTACCGTAGAAA
AAGCTAAAAATTATATAAAAAATTCTAACAGACTTGGCAGAACAAATAATTTAAAAACTA
TTGGTATTCTTCAAGAAGAGGTTTCATCTATGGAGGCTAAATCTATGACTTGGTAAAGAT
TTAGGTATAGAACTTGGTTCATTATTTTTTCTNAAGGAAAATACAACACTAAAAAAAAAA
TATGAAAATANAAT

ltk**milkkrlttlvll*lhflfqnln*rnsqsnkklylpntlqklvyri*qrslsqkn
*ilp*kklkii*kiltdlaeqii*kllvffkkrfhlwrlnl*lgkdlgielgslfflkki
kh*n*nmkik*NKANHLIVINKPSLYVKKK---

---XXIIXDIXKXANDSGVTLAPLSVPKPKLEELSEQQKIILAEYIAEVGLQNITAITLS
KKLNITVEKAKNYIKNSNRLGRTNNLKTIGILQEEVSSMEAKSMTW*rfryrtwfiifsx
gkyntkkki*kxn

Frame A:
t*qnnk*y*krg*rlwcyfsstfcskt*trgtlratknytcrihcrswfteynsdhslkk
ieyyrrks*klykkf*qtwqnk*fknywyssrrgfiygg*iydlvki*v*nlvhyff*kk
*ntkiki*k*nkiklii***ltnphct*kk---

---lxx*smilxkxlttlvll*lhflfqnln*rnsqsnkklylpntlqklvyri*qrsls
qkn*ilp*kklkii*kiltdlaeqii*kllvffkkrfhlwrlnl*lgkdlgielgslffl
kenttlkkkyenx

Frame B:
ldkiindikkeandsgvtlaplsvpkpkleelseqqkiilaeyiaevglqnitaitlskk
lnitvekaknyiknsnrlgrtnnlktigilqeevssmeaksmtw*rfryrtwfiifskkn
ktlklkyenkik*s*sfnsd*qtlivrkkk---

---xxxnx*yxkxg*rlwcyfsstfcskt*trgtlratknytcrihcrswfteynsdhsl
kkieyyrrks*klykkf*qtwqnk*fknywyssrrgfiygg*iydlvki*v*nlvhyffx
rkiqh*kknmkix

Frame C:
ltk**milkkrlttlvll*lhflfqnln*rnsqsnkklylpntlqklvyri*qrslsqkn
*ilp*kklkii*kiltdlaeqii*kllvffkkrfhlwrlnl*lgkdlgielgslfflkki
kh*n*nmkik*NKANHLIVINKPSLYVKKK---

---XXIIXDIXKXANDSGVTLAPLSVPKPKLEELSEQQKIILAEYIAEVGLQNITAITLS
KKLNITVEKAKNYIKNSNRLGRTNNLKTIGILQEEVSSMEAKSMTW*rfryrtwfiifsx
gkyntkkki*kxn

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSG453 (VSG453Q) /CSM/VS/VSG4-C/VSG453Q.Seq.d/                        343   3e-93
VSI252 (VSI252Q) /CSM/VS/VSI2-C/VSI252Q.Seq.d/                        341   1e-92
SLE177 (SLE177Q) /CSM/SL/SLE1-D/SLE177Q.Seq.d/                        270   3e-71
AHE844 (AHE844Q) /CSM/AH/AHE8-B/AHE844Q.Seq.d/                         38   0.21
AHE874 (AHE874Q) /CSM/AH/AHE8-D/AHE874Q.Seq.d/                         38   0.21
VHP767 (VHP767Q) /CSM/VH/VHP7-C/VHP767Q.Seq.d/                         38   0.21
VHP436 (VHP436Q) /CSM/VH/VHP4-B/VHP436Q.Seq.d/                         38   0.21
VHO880 (VHO880Q) /CSM/VH/VHO8-D/VHO880Q.Seq.d/                         38   0.21
VHO643 (VHO643Q) /CSM/VH/VHO6-B/VHO643Q.Seq.d/                         38   0.21

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC120008|AC120008.1 Mus musculus clone RP24-184M18, LOW-PASS SEQUENCE SAMPLING.    38  0.27  2
AC117070|AC117070.2 Dictyostelium discoideum chromosome 2 map 2097701-2233941 strain AX4, complete sequence.    40  0.29  7
AV741745|AV741745.1 Homo sapiens cDNA clone:CBLAUF11, 5'end, expressed in human CD34+ hematopoietic stem/progenitor cells.    36  0.50  2
AC011053|AC011053.4 Homo sapiens clone RP11-10G13, WORKING DRAFT SEQUENCE, 3 unordered pieces.    44  0.61  2
AC002368|AC002368.1 Homo sapiens Xq28 BAC PAC and cosmid clones containing FMR2 gene exons 1,2, and 3, complete sequence.    46  0.72  1
BZ349566|BZ349566.1 hr42c09.g1 WGS-SbicolorF (JM107 adapted methyl filtered) Sorghum bicolor genomic clone hr42c09 5', DNA sequence.    34  0.73  2
AC015544|AC015544.19 Homo sapiens chromosome 12 clone RP11-1007B5, WORKING DRAFT SEQUENCE, 7 unordered pieces.    44  0.88  3
AC136342|AC136342.1 Homo sapiens chromosome 18 clone RP13-9J14 map 18, LOW-PASS SEQUENCE SAMPLING.    36  1.3  3
AL359394|AL359394.9 Human DNA sequence from clone RP11-264M20 on chromosome 13. Contains ESTs, STSs and GSSs. Contains a novel pseudogene.    42  1.9  2
AX344573|AX344573.1 Sequence 24 from Patent WO0200932.            38  2.1  5

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BX548174_1381(BX548174|pid:none) Prochlorococcus marinus MED4 co...    35   2.9
(Q73BQ9) Indole-3-glycerol phosphate synthase (EC 4.1.1.48) (IGP...    35   2.9
AM285315_5(AM285315|pid:none) Spiroplasma citri GII3-3X chromoso...    35   3.8
T31826(T31826)hypothetical protein C17E7.3 - Caenorhabditis eleg...    34   5.0
AE000512_67(AE000512|pid:none) Thermotoga maritima MSB8, complet...    33   8.6
EU016580_13(EU016580|pid:none) Uncultured marine microorganism H...    33   8.6
FM992693_183(FM992693|pid:none) Candida dubliniensis CD36 chromo...    33   8.6

 psg: 0.53  gvh: 0.38  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 24.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VSG453 is cyt

>VSG453F.Seq
ACTTGACAAAATAATAAATGATATTAAAAAAGAGGCTAACGACTCTGGTGTTACTTTAGC
TCCACTTTCTGTTCCAAAACCTAAACTAGAGGAACTCTCAGAGCAACAAAAAATTATACT
TGCCGAATACATTGCAGAAGTTGGTTTACAGAATATAACAGCGATCACTCTCTCAAAAAA
ATTGAATATTACCGTAGAAAAAGCTAAAAATTATATAAAAAATTCTAACAGACTTGGCAG
AACAAATAATTTAAAAACTATTGGTATTCTTCAAGAAGAGGTTTCATCTATGGAGGCTAA
ATCTATGACTTGGTAAAGATTTAGGTATAGAACTTGGTTCATTATTTTTTCTAAAAAAAA
TAAAACACTAAAATTAAAATATGAAAATAAAATAAAATAAAGCTAATCATTTAATAGTGA
TTAACAAACCCTCATTGTACGTAAAAAAAAAA----------

>VSG453Z.Seq
----------TTGNCNNNATAATCNATGATATTANAAAAGNGGCTAACGACTCTGGTGTT
ACTTTAGCTCCACTTTCTGTTCCAAAACCTAAACTAGAGGAACTCTCAGAGCAACAAAAA
ATTATACTTGCCGAATACATTGCAGAAGTTGGTTTACAGAATATAACAGCGATCACTCTC
TCAAAAAAATTGAATATTACCGTAGAAAAAGCTAAAAATTATATAAAAAATTCTAACAGA
CTTGGCAGAACAAATAATTTAAAAACTATTGGTATTCTTCAAGAAGAGGTTTCATCTATG
GAGGCTAAATCTATGACTTGGTAAAGATTTAGGTATAGAACTTGGTTCATTATTTTTTCT
NAAGGAAAATACAACACTAAAAAAAAAATATGAAAATANAAT

>VSG453P.Seq
ACTTGACAAAATAATAAATGATATTAAAAAAGAGGCTAACGACTCTGGTGTTACTTTAGC
TCCACTTTCTGTTCCAAAACCTAAACTAGAGGAACTCTCAGAGCAACAAAAAATTATACT
TGCCGAATACATTGCAGAAGTTGGTTTACAGAATATAACAGCGATCACTCTCTCAAAAAA
ATTGAATATTACCGTAGAAAAAGCTAAAAATTATATAAAAAATTCTAACAGACTTGGCAG
AACAAATAATTTAAAAACTATTGGTATTCTTCAAGAAGAGGTTTCATCTATGGAGGCTAA
ATCTATGACTTGGTAAAGATTTAGGTATAGAACTTGGTTCATTATTTTTTCTAAAAAAAA
TAAAACACTAAAATTAAAATATGAAAATAAAATAAAATAAAGCTAATCATTTAATAGTGA
TTAACAAACCCTCATTGTACGTAAAAAAAAAA----------TTGNCNNNATAATCNATG
ATATTANAAAAGNGGCTAACGACTCTGGTGTTACTTTAGCTCCACTTTCTGTTCCAAAAC
CTAAACTAGAGGAACTCTCAGAGCAACAAAAAATTATACTTGCCGAATACATTGCAGAAG
TTGGTTTACAGAATATAACAGCGATCACTCTCTCAAAAAAATTGAATATTACCGTAGAAA
AAGCTAAAAATTATATAAAAAATTCTAACAGACTTGGCAGAACAAATAATTTAAAAACTA
TTGGTATTCTTCAAGAAGAGGTTTCATCTATGGAGGCTAAATCTATGACTTGGTAAAGAT
TTAGGTATAGAACTTGGTTCATTATTTTTTCTNAAGGAAAATACAACACTAAAAAAAAAA
TATGAAAATANAAT