NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSG423
Library VS
(Link to library)
Clone ID VSG423
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15350-1
Original site URL
Representative seq. ID VSG423E
(Link to Original site)
Representative DNA sequence 
>VSG423 (VSG423Q) /CSM/VS/VSG4-A/VSG423Q.Seq.d/
AGTTAAAATGGCAGAAAAAGAACAAATTAAATTAGTCTTAGTTGGTGATGGTGGTGTTGG
TAAAACCACTTTTGTCCAACGTCATCTTACTGGTGAATTCGAACCAAGATATATCCCAAC
TCTTGGTGTATCAGTCCATCCATTAATNTTCTACACCAATTTCGGTAAAATCCACTTCAA
TGTATGGGATACCGCTGGTCAAGAAAAATTCGGTGGTTTAANAGATGGTTATTATATTCA
AGGTAATTGTGCCATTATTATGTTTGATGTCACATCCCGTATTTCATACAAAAATGTTCC
AAATTGGCACTCTGATTTAACCAGAGTTTGTGAAAACATCCCAATCGTTTTATGTGGTAA
CAAAGTTGATGTTAAAGATCGTAAAGTCAAACCATCCCAAATTGTTTTCCATCGTAGATA
CAATTTATCATACTATGATGTTTCAGCCAAATCAAATTACAATTTCGAAAAACCATTCGT
CTGGTTAACATCAAAACTCCTCGGTAACAAGGCTGTCACTCTCGTTCAACAACCAACCTT
AAAATTACCANAAACCGNTCTCGATTCCNACTTAATGAGTTTANATGAAAAANAAGTCGC
TGANGCTGCTGCCCNCCCNTTACCAGAAGACAATGATGACNTGNAAACNAATTTAAAAAA
AAAAAACAATAAAAAAAAATAATAATAATAATAATAAAGCCAACAATAAATTTCAA
sequence update 2001. 3.25
Translated Amino Acid sequence 
VKMAEKEQIKLVLVGDGGVGKTTFVQRHLTGEFEPRYIPTLGVSVHPLXFYTNFGKIHFN
VWDTAGQEKFGGLXDGYYIQGNCAIIMFDVTSRISYKNVPNWHSDLTRVCENIPIVLCGN
KVDVKDRKVKPSQIVFHRRYNLSYYDVSAKSNYNFEKPFVWLTSKLLGNKAVTLVQQPTL
KLPXTXLDSXLMSLXEKXVAXAAAXPLPEDNDDXXTNLKKKNNKKK******sqq*is
Translated Amino Acid sequence (All Frames) 
Frame A:
s*ngrkrtn*islsw*wwcw*nhfcptssyw*irtkiypnswcispsinxlhqfr*nplq
cmgyrwsrkirwfxrwllysr*lchyyv*chipyfiqkcsklal*fnqsl*khpnrfmw*
qs*c*rs*sqtipncfps*iqfiil*cfsqiklqfrktirlvniktpr*qgchsrsttnl
kitxnrsrfxlnefx*kxsr*xccppxtrrq**xxnxfkkkkq*kkiiiiiikptinf


Frame B:
VKMAEKEQIKLVLVGDGGVGKTTFVQRHLTGEFEPRYIPTLGVSVHPLXFYTNFGKIHFN
VWDTAGQEKFGGLXDGYYIQGNCAIIMFDVTSRISYKNVPNWHSDLTRVCENIPIVLCGN
KVDVKDRKVKPSQIVFHRRYNLSYYDVSAKSNYNFEKPFVWLTSKLLGNKAVTLVQQPTL
KLPXTXLDSXLMSLXEKXVAXAAAXPLPEDNDDXXTNLKKKNNKKK******sqq*is


Frame C:
lkwqkknkln*s*lvmvvlvkpllsnvillvnsnqdisqllvyqsih*xstpisvkstsm
ygiplvkknsvv*xmviifkvivpllclmshpvfhtkmfqigtli*pefvktsqsfyvvt
klmlkivksnhpklfsivdtiyhtmmfqpnqitisknhssg*hqnssvtrlslsfnnqp*
nyxkpxsipt**vxmkxkslxllpxxyqktmmtxkxi*kkktikknnnnnnkannkfq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSG423 (VSG423Q) /CSM/VS/VSG4-A/VSG423Q.Seq.d/                       1219   0.0
SSI774 (SSI774Q) /CSM/SS/SSI7-D/SSI774Q.Seq.d/                       1219   0.0
SLA506 (SLA506Q) /CSM/SL/SLA5-A/SLA506Q.Seq.d/                       1219   0.0
VSD229 (VSD229Q) /CSM/VS/VSD2-B/VSD229Q.Seq.d/                       1205   0.0
VSI212 (VSI212Q) /CSM/VS/VSI2-A/VSI212Q.Seq.d/                       1179   0.0
AHG822 (AHG822Q) /CSM/AH/AHG8-A/AHG822Q.Seq.d/                       1172   0.0
VSI638 (VSI638Q) /CSM/VS/VSI6-B/VSI638Q.Seq.d/                       1164   0.0
VSG164 (VSG164Q) /CSM/VS/VSG1-C/VSG164Q.Seq.d/                       1160   0.0
VSG244 (VSG244Q) /CSM/VS/VSG2-B/VSG244Q.Seq.d/                       1104   0.0
VSF607 (VSF607Q) /CSM/VS/VSF6-A/VSF607Q.Seq.d/                       1102   0.0
own update 2003. 1. 9
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

L09720|L09720.1 Dictyostelium discoideum GTP-binding protein (TC4/RAN) mRNA, complete cds.  1219   0.0    1
AX489114|AX489114.1 Sequence 6414 from Patent WO02053728.         115   5e-53  4
AF190700|AF190700.1 Candida albicans small G-protein Gsp1p (GSP1) gene, complete cds.   115   5e-51  3
CA758831|AAF60289.1, mRNA sequence.    74   1e-50  5
BM275423|BM275423.1 PfESToaa68f04.y1 Plasmodium falciparum 3D7 gametocyte cDNA library Plasmodium falciparum 3D7 cDNA 5' similar to SW:RAN_PLAFA P38545 GTP-BINDING NUCLEAR PROTEIN RAN/TC4. ;, mRNA sequence.    86   4e-38  5
BU498261|BU498261.1 PfESToab94h09.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to SW:RAN_PLAFA P38545 GTP-BINDING NUCLEAR PROTEIN RAN/TC4. ;, mRNA sequence.    86   4e-38  5
BM273404|BM273404.1 PfESToaa50c11.y1 Plasmodium falciparum 3D7 gametocyte cDNA library Plasmodium falciparum 3D7 cDNA 5' similar to SW:RAN_PLAFA P38545 GTP-BINDING NUCLEAR PROTEIN RAN/TC4. ;, mRNA sequence.    86   4e-38  5
BI815642|BI815642.1 PfESToaa31a10.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to SW:RAN_PLAFA P38545 GTP-BINDING NUCLEAR PROTEIN RAN/TC4. ;, mRNA sequence.    86   4e-38  5
BI815554|BI815554.1 PfESToaa29h11.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to SW:RAN_PLAFA P38545 GTP-BINDING NUCLEAR PROTEIN RAN/TC4. ;, mRNA sequence.    86   4e-38  5
BQ452295|BQ452295.1 PfESToaa94h01.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to SW:RAN_PLAFA P38545 GTP-BINDING NUCLEAR PROTEIN RAN/TC4. ;, mRNA sequence.    86   4e-38  5
dna update 2003.11.20
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P33519) RecName: Full=GTP-binding nuclear protein Ran; AltName:...   391   e-108
(O17915) RecName: Full=GTP-binding nuclear protein ran-1; AltNam...   286   5e-76
CP000499_469(CP000499|pid:none) Pichia stipitis CBS 6054 chromos...   284   2e-75
CR382138_83(CR382138|pid:none) Debaryomyces hansenii strain CBS7...   284   2e-75
(P32836) RecName: Full=GTP-binding nuclear protein GSP2/CNR2;  &...   283   3e-75
EF103373_1(EF103373|pid:none) Haliotis discus discus Ran-1-prov ...   283   4e-75
(Q74ZA9) RecName: Full=GTP-binding nuclear protein GSP1/Ran;  &A...   283   5e-75
CR382121_184(CR382121|pid:none) Kluyveromyces lactis strain NRRL...   283   5e-75
JC1455(JC1455;S20712)GTP-binding protein Ran/TC4 - dog                283   5e-75
CU633438_787(CU633438|pid:none) Podospora anserina genomic DNA c...   282   6e-75
protein update 2009. 3.23
PSORT 

 psg: 0.80  gvh: 0.35  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VSG423 is nuc
5' end seq. ID VSG423F
5' end seq. 
>VSG423F.Seq
AGTTAAAATGGCAGAAAAAGAACAAATTAAATTAGTCTTAGTTGGTGATGGTGGTGTTGG
TAAAACCACTTTTGTCCAACGTCATCTTACTGGTGAATTCGAACCAAGATATATCCCAAC
TCTTGGTGTATCAGTCCATCCATTAATCTTCTACACCAATTTCGGTAAAATCCACTTCAA
TGTATGGGATACCGCTGGTCAAGAAAAATTCGGTGGTTTAAGAGATGGTTATTATATTCA
AGGTAATTGTGCCATTATTATGTTTGATGTCACATCCCGTATTTCATACAAAAATGTTCC
AAATTGGCACTCTGATTTAACCAGAGTTTGTGAAAACATCCCAATCGTTTTATGTGGTAA
CAAAGTTGATGTTAAAGATCGTAAAGTCAAACCATCCCAAATTGTTTTCCATCGTAGATA
CAATTTATCATACTATGATGTTTCAGCCAAATCAAATTACAATTTCGAAAAACCATTCGT
CTGGTTAACATCAAAACTCCTCGGTAACAAGGCTGTCACT----------
Length of 5' end seq. 520
3' end seq. ID VSG423Z
3' end seq. 
>VSG423Z.Seq
----------GTGNATCAGTCCATCCATTAATTTTNTACACCAATTTCGGTAAAATCCNC
TTCAATGTATGGGANACCNCTGGTCAANAAAAATTCGGTGGTTTAAAANATGGTTATTAT
ATTCAAGGTAATTGNGCCATTATTATGTTTGATGTCACATCCCGTATTTNANACAAAAAT
GTTCCAAATTGGCNCTNTGATTTAACCANAGTTTGTGAAAACATCCCAATNGTTTTATGT
GGTAACAAAGTTNATGTTAAANATNGTAAAGTCAAACCATCCCAAATTGTTTTCCATNGT
ANANACAATTTATCATACTATGATGTTTCAGCCAAATCAAATTACAATTTNGAAAAACCA
TTNGTNTGGTTAACATCAAAACNCCNCGGNAACAAGGCTGTCACTCTCGTTCAACAACCA
ACCTTAAAATTACCANAAACCGNTCTCGATTCCNACTTAATGAGTTTANATGAAAAANAA
GTCGCTGANGCTGCTGCCCNCCCNTTACCAGAAGACAATGATGACNTGNAAACNAATTTA
AAAAAAAAAAACAATAAAAAAAAATAATAATAATAATAATAAAGCCAACAATAAATTTCA
A
Length of 3' end seq. 591
Connected seq. ID VSG423P
Connected seq. 
>VSG423P.Seq
AGTTAAAATGGCAGAAAAAGAACAAATTAAATTAGTCTTAGTTGGTGATGGTGGTGTTGG
TAAAACCACTTTTGTCCAACGTCATCTTACTGGTGAATTCGAACCAAGATATATCCCAAC
TCTTGGTGTATCAGTCCATCCATTAATCTTCTACACCAATTTCGGTAAAATCCACTTCAA
TGTATGGGATACCGCTGGTCAAGAAAAATTCGGTGGTTTAAGAGATGGTTATTATATTCA
AGGTAATTGTGCCATTATTATGTTTGATGTCACATCCCGTATTTCATACAAAAATGTTCC
AAATTGGCACTCTGATTTAACCAGAGTTTGTGAAAACATCCCAATCGTTTTATGTGGTAA
CAAAGTTGATGTTAAAGATCGTAAAGTCAAACCATCCCAAATTGTTTTCCATCGTAGATA
CAATTTATCATACTATGATGTTTCAGCCAAATCAAATTACAATTTCGAAAAACCATTCGT
CTGGTTAACATCAAAACTCCTCGGTAACAAGGCTGTCACT----------GTGNATCAGT
CCATCCATTAATTTTNTACACCAATTTCGGTAAAATCCNCTTCAATGTATGGGANACCNC
TGGTCAANAAAAATTCGGTGGTTTAAAANATGGTTATTATATTCAAGGTAATTGNGCCAT
TATTATGTTTGATGTCACATCCCGTATTTNANACAAAAATGTTCCAAATTGGCNCTNTGA
TTTAACCANAGTTTGTGAAAACATCCCAATNGTTTTATGTGGTAACAAAGTTNATGTTAA
ANATNGTAAAGTCAAACCATCCCAAATTGTTTTCCATNGTANANACAATTTATCATACTA
TGATGTTTCAGCCAAATCAAATTACAATTTNGAAAAACCATTNGTNTGGTTAACATCAAA
ACNCCNCGGNAACAAGGCTGTCACTCTCGTTCAACAACCAACCTTAAAATTACCANAAAC
CGNTCTCGATTCCNACTTAATGAGTTTANATGAAAAANAAGTCGCTGANGCTGCTGCCCN
CCCNTTACCAGAAGACAATGATGACNTGNAAACNAATTTAAAAAAAAAAAACAATAAAAA
AAAATAATAATAATAATAATAAAGCCAACAATAAATTTCAA
Length of connected seq. 1111
Full length Seq ID VSG423E
Full length Seq. 
>VSG423E.Seq
AGTTAAAATGGCAGAAAAAGAACAAATTAAATTAGTCTTAGTTGGTGATGGTGGTGTTGG
TAAAACCACTTTTGTCCAACGTCATCTTACTGGTGAATTCGAACCAAGATATATCCCAAC
TCTTGGTGTATCAGTCCATCCATTAATNTTCTACACCAATTTCGGTAAAATCCACTTCAA
TGTATGGGATACCGCTGGTCAAGAAAAATTCGGTGGTTTAANAGATGGTTATTATATTCA
AGGTAATTGTGCCATTATTATGTTTGATGTCACATCCCGTATTTCATACAAAAATGTTCC
AAATTGGCACTCTGATTTAACCAGAGTTTGTGAAAACATCCCAATCGTTTTATGTGGTAA
CAAAGTTGATGTTAAAGATCGTAAAGTCAAACCATCCCAAATTGTTTTCCATCGTAGATA
CAATTTATCATACTATGATGTTTCAGCCAAATCAAATTACAATTTCGAAAAACCATTCGT
CTGGTTAACATCAAAACTCCTCGGTAACAAGGCTGTCACTCTCGTTCAACAACCAACCTT
AAAATTACCANAAACCGNTCTCGATTCCNACTTAATGAGTTTANATGAAAAANAAGTCGC
TGANGCTGCTGCCCNCCCNTTACCAGAAGACAATGATGACNTGNAAACNAATTTAAAAAA
AAAAAACAATAAAAAAAAATAATAATAATAATAATAAAGCCAACAATAAATTTCAA
Length of full length seq. 716