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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSG353
Library VS
(Link to library)
Clone ID VSG353
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14495-1
Original site URL
Representative seq. ID VSG353P
(Link to Original site)
Representative DNA sequence 
>VSG353 (VSG353Q) /CSM/VS/VSG3-C/VSG353Q.Seq.d/
AGTCTTTATAAACTTACACAATGTCAATGAACCCAATCAAAAAAACCGAAGAAACCGTTG
CAGCCCCAGCTGTCAACAAAATCAGTATCACCTTAACCTCAAGAAACCCAAAATCTCTTG
AAAAAGTTTGCGCTGATATCATCACTCTTGCTAAAAACAAGAAAGTCAAGGCTAAGGGTC
CAATCCGTATCCCAAACAAAGTCTTAAAAATCACCACCCGTAAAAGTCCATGTGGTGAAG
GTACAAACACCTGGGATCGTTTCGAAATGAGAATCCACAAACGTGTCATCCACATCTTAT
CCACCCAAGATTTCGTCAAGGAAATGAACACCATCTCAATCGAAGCTGGCGTTGATGTTG
AAGTCATCATGGATAAAAAAGAAGCCAAAAACTAAATTAATTAATCTTTAATTTATTAAT
AAACTTTTTTTATTATTTATAAAAAAAAAAXXXXXXXXXXAGTTGGGNTNCGGGNCCCCC
CTTNAGGTNNACCCACGNGTNCNAGTCTTTATAAACTTNCACAATGTCAATGAACCCAAT
CAAAAAAACCGAAGAAACCGTTGCAGCCCCAGCTGTCAACAAAATCAGTATCACCTTAAC
CTCAAGAAACCCAAAATCTCTTGAAAAAGTTTGCGCTGATATCATCACTCTTGCTAAAAA
CAAGAAAGTCAAGGNTAAGGGTCCAATCCGTATCCCAAACAAAGTCTTAAAAATCACCAC
CCGTAAAAGTCCATGTGGTGAAGGTACAAACACCTGGGATCGTTTCGAAATGAGAATCCA
CAAACGTGTCATCCACATCTTATCCACCCAAGATTTCGTCAAGGAAATGAACACCATCTC
AATCGAAGCTGGCGTTGATGTTGAAGTCATCATGAATAAAAAAGAAGCCAAAAACTAGGA
TTAATTA
sequence update 2001. 3.22
Translated Amino Acid sequence 
slykltqcq*tqskkpkkplqpqlstksvsp*pqetqnllkkfalisslllktrksrlrv
qsvsqtks*ksppvkvhvvkvqtpgivsk*estnvsstsyppkissrk*tpsqsklalml
ksswikkkpktklinl*FINKLFLLFIKKK---

---LGXGXPLXVXPRVXVFINXHNVNEPNQKNRRNRCSPSCQQNQYHLNLKKPKIS*ksl
r*yhhsc*kqesqg*gsnpypkqslknhhp*ksmw*rykhlgsfrnenpqtchphlihpr
frqgnehhlnrswr*c*shhe*krsqklgli
Translated Amino Acid sequence (All Frames) 
Frame A:
slykltqcq*tqskkpkkplqpqlstksvsp*pqetqnllkkfalisslllktrksrlrv
qsvsqtks*ksppvkvhvvkvqtpgivsk*estnvsstsyppkissrk*tpsqsklalml
ksswikkkpktklinl*FINKLFLLFIKKK---

---swxxgppxrxthxxxsl*txtmsmnpikkteetvaapavnkisitltsrnpkslekv
cadiitlaknkkvkxkgpiripnkvlkittrkspcgegtntwdrfemrihkrvihilstq
dfvkemntisieagvdvevimnkkeakn*d*l

Frame B:
vfinlhnvnepnqknrrnrcspscqqnqyhlnlkkpkis*kslr*yhhsc*kqesqg*gs
npypkqslknhhp*ksmw*rykhlgsfrnenpqtchphlihprfrqgnehhlnrswr*c*
shhg*krsqkln*lifnllinffyyl*kk---

---vgxrxppxgxptxxslyklxqcq*tqskkpkkplqpqlstksvsp*pqetqnllkkf
alisslllktrksrxrvqsvsqtks*ksppvkvhvvkvqtpgivsk*estnvsstsyppk
issrk*tpsqsklalmlkss*ikkkpktrin

Frame C:
sl*tytmsmnpikkteetvaapavnkisitltsrnpkslekvcadiitlaknkkvkakgp
iripnkvlkittrkspcgegtntwdrfemrihkrvihilstqdfvkemntisieagvdve
vimdkkeakn*in*sliy**tffiiykkk---

---LGXGXPLXVXPRVXVFINXHNVNEPNQKNRRNRCSPSCQQNQYHLNLKKPKIS*ksl
r*yhhsc*kqesqg*gsnpypkqslknhhp*ksmw*rykhlgsfrnenpqtchphlihpr
frqgnehhlnrswr*c*shhe*krsqklgli
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ856 (VSJ856Q) /CSM/VS/VSJ8-C/VSJ856Q.Seq.d/                        831   0.0
VSG353 (VSG353Q) /CSM/VS/VSG3-C/VSG353Q.Seq.d/                        831   0.0
VSE290 (VSE290Q) /CSM/VS/VSE2-D/VSE290Q.Seq.d/                        831   0.0
VSC595 (VSC595Q) /CSM/VS/VSC5-D/VSC595Q.Seq.d/                        831   0.0
VSB807 (VSB807Q) /CSM/VS/VSB8-A/VSB807Q.Seq.d/                        831   0.0
SHA481 (SHA481Q) /CSM/SH/SHA4-D/SHA481Q.Seq.d/                        831   0.0
VFM670 (VFM670Q) /CSM/VF/VFM6-C/VFM670Q.Seq.d/                        809   0.0
VSD308 (VSD308Q) /CSM/VS/VSD3-A/VSD308Q.Seq.d/                        807   0.0
VSB785 (VSB785Q) /CSM/VS/VSB7-D/VSB785Q.Seq.d/                        801   0.0
VSJ493 (VSJ493Q) /CSM/VS/VSJ4-D/VSJ493Q.Seq.d/                        799   0.0
own update 2003. 1. 9
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BP117144|BP117144.1 Bombyx mori cDNA, clone:brS-1313.             86  9e-13  1
AV399798|AV399798.1 Bombyx mori cDNA clone:NV120797.              86  9e-13  1
AL115017|AL115017.1 Botrytis cinerea strain T4 cDNA library under conditions of nitrogen deprivation.    74  2e-11  2
AL111409|AL111409.1 Botrytis cinerea strain T4 cDNA library under conditions of nitrogen deprivation.    74  2e-11  2
AL116620|AL116620.1 Botrytis cinerea strain T4 cDNA library under conditions of nitrogen deprivation.    74  3e-11  2
AL115591|AL115591.1 Botrytis cinerea strain T4 cDNA library under conditions of nitrogen deprivation.    74  3e-11  2
AL114779|AL114779.1 Botrytis cinerea strain T4 cDNA library under conditions of nitrogen deprivation.    74  3e-11  2
AL112608|AL112608.1 Botrytis cinerea strain T4 cDNA library under conditions of nitrogen deprivation.    74  3e-11  2
AL111708|AL111708.1 Botrytis cinerea strain T4 cDNA library under conditions of nitrogen deprivation.    74  3e-11  2
AT001633|AT001633.1 Bombyx mori egg EST clone E1.                 78  2e-10  1
dna update 2003. 8.25
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54Y41) RecName: Full=40S ribosomal protein S20;                     246   9e-64
(Q9STY6) RecName: Full=40S ribosomal protein S20-2;  &AK227448_1...   146   1e-33
CP000583_102(CP000583|pid:none) Ostreococcus lucimarinus CCE9901...   144   3e-33
AM427997_1(AM427997|pid:none) Vitis vinifera contig VV78X043700....   142   1e-32
AK062522_1(AK062522|pid:none) Oryza sativa Japonica Group cDNA c...   141   3e-32
EU302580_1(EU302580|pid:none) Lineus viridis ribosomal protein r...   140   7e-32
AB019235_13(AB019235|pid:none) Arabidopsis thaliana genomic DNA,...   139   9e-32
AY758353_1(AY758353|pid:none) Zea mays ribosomal protein S10p/S2...   139   1e-31
EU116931_1(EU116931|pid:none) Barentsia elongata putative riboso...   139   1e-31
EU952622_1(EU952622|pid:none) Zea mays clone 1284507 40S ribosom...   138   2e-31
protein update 2009. 3.23
PSORT 

 psg: 0.62  gvh: 0.46  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.07  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	 20.0 %: mitochondrial
	  4.0 %: endoplasmic reticulum

>> prediction for VSG353 is cyt
5' end seq. ID VSG353F
5' end seq. 
>VSG353F.Seq
AGTCTTTATAAACTTACACAATGTCAATGAACCCAATCAAAAAAACCGAAGAAACCGTTG
CAGCCCCAGCTGTCAACAAAATCAGTATCACCTTAACCTCAAGAAACCCAAAATCTCTTG
AAAAAGTTTGCGCTGATATCATCACTCTTGCTAAAAACAAGAAAGTCAAGGCTAAGGGTC
CAATCCGTATCCCAAACAAAGTCTTAAAAATCACCACCCGTAAAAGTCCATGTGGTGAAG
GTACAAACACCTGGGATCGTTTCGAAATGAGAATCCACAAACGTGTCATCCACATCTTAT
CCACCCAAGATTTCGTCAAGGAAATGAACACCATCTCAATCGAAGCTGGCGTTGATGTTG
AAGTCATCATGGATAAAAAAGAAGCCAAAAACTAAATTAATTAATCTTTAATTTATTAAT
AAACTTTTTTTATTATTTATAAAAAAAAAA----------
Length of 5' end seq. 450
3' end seq. ID VSG353Z
3' end seq. 
>VSG353Z.Seq
----------AGTTGGGNTNCGGGNCCCCCCTTNAGGTNNACCCACGNGTNCNAGTCTTT
ATAAACTTNCACAATGTCAATGAACCCAATCAAAAAAACCGAAGAAACCGTTGCAGCCCC
AGCTGTCAACAAAATCAGTATCACCTTAACCTCAAGAAACCCAAAATCTCTTGAAAAAGT
TTGCGCTGATATCATCACTCTTGCTAAAAACAAGAAAGTCAAGGNTAAGGGTCCAATCCG
TATCCCAAACAAAGTCTTAAAAATCACCACCCGTAAAAGTCCATGTGGTGAAGGTACAAA
CACCTGGGATCGTTTCGAAATGAGAATCCACAAACGTGTCATCCACATCTTATCCACCCA
AGATTTCGTCAAGGAAATGAACACCATCTCAATCGAAGCTGGCGTTGATGTTGAAGTCAT
CATGAATAAAAAAGAAGCCAAAAACTAGGATTAATTA
Length of 3' end seq. 447
Connected seq. ID VSG353P
Connected seq. 
>VSG353P.Seq
AGTCTTTATAAACTTACACAATGTCAATGAACCCAATCAAAAAAACCGAAGAAACCGTTG
CAGCCCCAGCTGTCAACAAAATCAGTATCACCTTAACCTCAAGAAACCCAAAATCTCTTG
AAAAAGTTTGCGCTGATATCATCACTCTTGCTAAAAACAAGAAAGTCAAGGCTAAGGGTC
CAATCCGTATCCCAAACAAAGTCTTAAAAATCACCACCCGTAAAAGTCCATGTGGTGAAG
GTACAAACACCTGGGATCGTTTCGAAATGAGAATCCACAAACGTGTCATCCACATCTTAT
CCACCCAAGATTTCGTCAAGGAAATGAACACCATCTCAATCGAAGCTGGCGTTGATGTTG
AAGTCATCATGGATAAAAAAGAAGCCAAAAACTAAATTAATTAATCTTTAATTTATTAAT
AAACTTTTTTTATTATTTATAAAAAAAAAA----------AGTTGGGNTNCGGGNCCCCC
CTTNAGGTNNACCCACGNGTNCNAGTCTTTATAAACTTNCACAATGTCAATGAACCCAAT
CAAAAAAACCGAAGAAACCGTTGCAGCCCCAGCTGTCAACAAAATCAGTATCACCTTAAC
CTCAAGAAACCCAAAATCTCTTGAAAAAGTTTGCGCTGATATCATCACTCTTGCTAAAAA
CAAGAAAGTCAAGGNTAAGGGTCCAATCCGTATCCCAAACAAAGTCTTAAAAATCACCAC
CCGTAAAAGTCCATGTGGTGAAGGTACAAACACCTGGGATCGTTTCGAAATGAGAATCCA
CAAACGTGTCATCCACATCTTATCCACCCAAGATTTCGTCAAGGAAATGAACACCATCTC
AATCGAAGCTGGCGTTGATGTTGAAGTCATCATGAATAAAAAAGAAGCCAAAAACTAGGA
TTAATTA
Length of connected seq. 897
Full length Seq ID -
Full length Seq. -
Length of full length seq. -