>VSG302 (VSG302Q) /CSM/VS/VSG3-A/VSG302Q.Seq.d/
GNACACTCTAATAGTAGTTAATTGTGTAATATTAAATAAATATTATTATTTTGTACCATT
ACTTTATAAACAAAAAAAAAAAACCAAAAAAAAAACCAAAAAAAAAAXXXXXXXXXXANN
NAAAAAAAAANTTTTTTTTNNGNCCCCCNTTTTTTTTCCCNAAAAAAAAAACCCNAAAAA
AANCCCNAAAAAAAAAAAAAAAAAAAAAAATTTTTTTTTTTTTTANCCCCNTTNNAAAAC
CNNCCNTTTTTTCCNNNAAAAAAAAAAAAAAAAAAAAAATTAT

XTLIVVNCVILNKYYYFVPLLYKQKKKTKKKTKKK---

---XXKKXFFXXPXFFSXKKKPXKXPXKKKKKKKFFFFLXPXXNXPFFPXKKKKKKKL

Frame A:
xhsnss*lcnik*illfctitl*tkkknqkknqkk---

---XXKKXFFXXPXFFSXKKKPXKXPXKKKKKKKFFFFLXPXXNXPFFPXKKKKKKKL

Frame B:
XTLIVVNCVILNKYYYFVPLLYKQKKKTKKKTKKK---

---xkkkxffxxpxffpxkknpkkxpkkkkkkknffffxpxxktxxffxxkkkkkkny

Frame C:
tl***liv*y*iniiilyhyfinkkkkpkkkpkkk---

---xkkxffxxppfffpkkktxkkxxkkkkkkkiffffxpxxkpxxfsxkkkkkkki

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSG302 (VSG302Q) /CSM/VS/VSG3-A/VSG302Q.Seq.d/                        137   1e-31
VSF724 (VSF724Q) /CSM/VS/VSF7-A/VSF724Q.Seq.d/                         42   0.004
VFM236 (VFM236Q) /CSM/VF/VFM2-B/VFM236Q.Seq.d/                         42   0.004
VFE253 (VFE253Q) /CSM/VF/VFE2-C/VFE253Q.Seq.d/                         42   0.004
AFL586 (AFL586Q) /CSM/AF/AFL5-D/AFL586Q.Seq.d/                         42   0.004
VSF831 (VSF831Q) /CSM/VS/VSF8-B/VSF831Q.Seq.d/                         36   0.27
VSD675 (VSD675Q) /CSM/VS/VSD6-D/VSD675Q.Seq.d/                         36   0.27
VHM693 (VHM693Q) /CSM/VH/VHM6-D/VHM693Q.Seq.d/                         36   0.27
VHH739 (VHH739Q) /CSM/VH/VHH7-B/VHH739Q.Seq.d/                         36   0.27
VHE410 (VHE410Q) /CSM/VH/VHE4-A/VHE410Q.Seq.d/                         36   0.27

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AL035477|AL035477.6 Plasmodium falciparum MAL4P4.                 50  0.014  1
AL355979|AL355979.11 Human DNA sequence from clone RP11-466G24 on chromosome 10.    50  0.048  2
AF242881|AF242881.1 Agrobacterium tumefaciens octopine-type Ti plasmid, complete sequence.    46  0.23  1
CC699547|CC699547.1 OGVBQ79TH ZM_0.7_1.5_KB Zea mays genomic clone ZMMBMa0497N13, genomic survey sequence.    46  0.23  1
BD251969|BD251969.1 Artificial matrix attachment region for increasing expression of genes introduced in plant cells.    46  0.23  1
BD251970|BD251970.1 Artificial matrix attachment region for increasing expression of genes introduced in plant cells.    46  0.23  1
BZ365749|BZ365749.1 ie28g08.g1 WGS-ZmaysF (JM107 adapted methyl filtered) Zea mays genomic clone ie28g08 5', DNA sequence.    46  0.23  1
BD132698|BD132698.1 Regulatory sequences for transgenic plants.    46  0.23  1
BD132699|BD132699.1 Regulatory sequences for transgenic plants.    46  0.23  1
AR209002|AR209002.1 Sequence 9 from patent US 6384207.            46  0.23  1

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3019103
Number of Hits to DB: 86,672,126

 psg: 0.90  gvh: 0.28  alm: 0.39  top: 0.80  tms: 0.00  mit: 0.40  mip: 0.00
 nuc: 0.92  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 72.0 %: nuclear
	 20.0 %: cytoplasmic
	  4.0 %: mitochondrial
	  4.0 %: plasma membrane

>> prediction for VSG302 is nuc

>VSG302F.Seq
GNACACTCTAATAGTAGTTAATTGTGTAATATTAAATAAATATTATTATTTTGTACCATT
ACTTTATAAACAAAAAAAAAAAACCAAAAAAAAAACCAAAAAAAAAA----------

>VSG302Z.Seq
----------ANNNAAAAAAAAANTTTTTTTTNNGNCCCCCNTTTTTTTTCCCNAAAAAA
AAAACCCNAAAAAAANCCCNAAAAAAAAAAAAAAAAAAAAAAATTTTTTTTTTTTTTANC
CCCNTTNNAAAACCNNCCNTTTTTTCCNNNAAAAAAAAAAAAAAAAAAAAAATTAT

>VSG302P.Seq
GNACACTCTAATAGTAGTTAATTGTGTAATATTAAATAAATATTATTATTTTGTACCATT
ACTTTATAAACAAAAAAAAAAAACCAAAAAAAAAACCAAAAAAAAAA----------ANN
NAAAAAAAAANTTTTTTTTNNGNCCCCCNTTTTTTTTCCCNAAAAAAAAAACCCNAAAAA
AANCCCNAAAAAAAAAAAAAAAAAAAAAAATTTTTTTTTTTTTTANCCCCNTTNNAAAAC
CNNCCNTTTTTTCCNNNAAAAAAAAAAAAAAAAAAAAAATTAT