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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSG261
Library VS
(Link to library)
Clone ID VSG261
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15984-1
Original site URL
Representative seq. ID VSG261P
(Link to Original site)
Representative DNA sequence 
>VSG261 (VSG261Q) /CSM/VS/VSG2-C/VSG261Q.Seq.d/
GTTTATGTTGCTTATGAATACATTGGTTATGTTTTACCATTTGCTGCTGATCTCTTATAC
AATGGTAAGTTCCCACATTTAGATTCTTTGAAAGAACATTTCGAATCTAATAAAGGTGTA
GCAGAATATCTCAAAAATAGACCAATTACTGAAAGAAAAATTTAAGAAAAAAAAAAXXXX
XXXXXXAATAAAAAAATAAAAAATAAAAATATAAATTAAAAATAAAAAAAAAGTAAACNA
CANCAACAACAACAANGCTTCAATTATTACCTTTTTT
sequence update 2001. 3.22
Translated Amino Acid sequence 
fmllmntlvmfyhlllisytmvsshi*il*knisnlikv*QNISKIDQLLKEKFKKKK--
-

---NKKIKNKNIN*k*kkskxxqqqqxfnyylf
Translated Amino Acid sequence (All Frames) 
Frame A:
vyvayeyigyvlpfaadllyngkfphldslkehfesnkgvaeylknrpiterki*ekk--
-

---NKKIKNKNIN*k*kkskxxqqqqxfnyylf

Frame B:
fmllmntlvmfyhlllisytmvsshi*il*knisnlikv*QNISKIDQLLKEKFKKKK--
-

---ikk*kiki*iknkkkvnxxnnnxasiitff

Frame C:
lccl*ihwlcfticc*sliqw*vptfrffertfri**rcsrisqk*tny*kknlrkkk--
-

---*knkk*kyklkikkk*ttxtttxlqllpf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSG484 (VSG484Q) /CSM/VS/VSG4-D/VSG484Q.Seq.d/                        329   1e-89
VSG261 (VSG261Q) /CSM/VS/VSG2-C/VSG261Q.Seq.d/                        329   1e-89
VSE537 (VSE537Q) /CSM/VS/VSE5-B/VSE537Q.Seq.d/                        329   1e-89
VSE486 (VSE486Q) /CSM/VS/VSE4-D/VSE486Q.Seq.d/                        329   1e-89
VSD160 (VSD160Q) /CSM/VS/VSD1-C/VSD160Q.Seq.d/                        329   1e-89
VSB879 (VSB879Q) /CSM/VS/VSB8-D/VSB879Q.Seq.d/                        329   1e-89
VSB485 (VSB485Q) /CSM/VS/VSB4-D/VSB485Q.Seq.d/                        329   1e-89
SSA686 (SSA686Q) /CSM/SS/SSA6-D/SSA686Q.Seq.d/                        329   1e-89
SSC544 (SSC544Q) /CSM/SS/SSC5-B/SSC544Q.Seq.d/                        317   5e-86
VHM467 (VHM467Q) /CSM/VH/VHM4-C/VHM467Q.Seq.d/                        285   2e-76
own update 2003. 1. 9
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    36  0.087  4
AP002408|AP002408.2 Homo sapiens genomic DNA, chromosome 18q21 clone:RP11-779L15, WORKING DRAFT SEQUENCE, 22 unordered pieces.    38  0.58  4
AC122022|AC122022.4 Mus musculus chromosome 5 clone RP24-372J5, complete sequence.    44  0.87  1
AC022452|AC022452.3 Mus musculus chromosome 5 clone RP23-141N6 strain C57BL6/J, WORKING DRAFT SEQUENCE, 19 unordered pieces.    44  0.87  1
AL161727|AL161727.15 Human DNA sequence from clone RP11-232A1 on chromosome 9.    36  0.97  3
AC021325|AC021325.5 Homo sapiens chromosome , clone RP11-23N15, complete sequence.    38  2.3  5
AL096700|AL096700.14 Human DNA sequence from clone RP3-499B10 on chromosome  Xp22.31-22.13. Contains the 3' end of the gene for serine/threonine  protein phosphatase PPEF, the 3' end of the PHKA2 gene for liver  phosphorylase kinase alpha 2, ESTs, STSs and GSSs.    42  2.4  2
AC124826|AC124826.4 Mus musculus clone RP23-455F18, WORKING DRAFT SEQUENCE, 3 unordered pieces.    42  3.4  1
AC034283|AC034283.3 Homo sapiens chromosome 18 clone RP11-762K20 map 18, WORKING DRAFT SEQUENCE, 31 unordered pieces.    42  3.4  1
AL596323|AL596323.10 Human DNA sequence from clone RP11-83O12 on chromosome 9p13.3-21.    38  4.4  3
dna update 2003. 8.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54VI4) RecName: Full=Putative glutathione S-transferase alpha-...   114   8e-25
(Q86AU1) RecName: Full=Putative glutathione S-transferase alpha-...    60   2e-08
(Q55FF3) RecName: Full=Putative glutathione S-transferase alpha-...    57   2e-07
(Q556G3) RecName: Full=Putative glutathione S-transferase alpha-...    44   0.002
(Q54QV7) RecName: Full=Putative glutathione S-transferase alpha-...    35   0.78
protein update 2009. 3.22
PSORT 

 psg: 0.20  gvh: -0.09  alm: 0.81  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.28  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 68.0 %: nuclear
	 16.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for VSG261 is nuc
5' end seq. ID VSG261F
5' end seq. 
>VSG261F.Seq
GTTTATGTTGCTTATGAATACATTGGTTATGTTTTACCATTTGCTGCTGATCTCTTATAC
AATGGTAAGTTCCCACATTTAGATTCTTTGAAAGAACATTTCGAATCTAATAAAGGTGTA
GCAGAATATCTCAAAAATAGACCAATTACTGAAAGAAAAATTTAAGAAAAAAAAAA----
------
Length of 5' end seq. 176
3' end seq. ID VSG261Z
3' end seq. 
>VSG261Z.Seq
----------AATAAAAAAATAAAAAATAAAAATATAAATTAAAAATAAAAAAAAAGTAA
ACNACANCAACAACAACAANGCTTCAATTATTACCTTTTTT
Length of 3' end seq. 91
Connected seq. ID VSG261P
Connected seq. 
>VSG261P.Seq
GTTTATGTTGCTTATGAATACATTGGTTATGTTTTACCATTTGCTGCTGATCTCTTATAC
AATGGTAAGTTCCCACATTTAGATTCTTTGAAAGAACATTTCGAATCTAATAAAGGTGTA
GCAGAATATCTCAAAAATAGACCAATTACTGAAAGAAAAATTTAAGAAAAAAAAAA----
------AATAAAAAAATAAAAAATAAAAATATAAATTAAAAATAAAAAAAAAGTAAACNA
CANCAACAACAACAANGCTTCAATTATTACCTTTTTT
Length of connected seq. 267
Full length Seq ID -
Full length Seq. -
Length of full length seq. -