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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSG224
Library VS
(Link to library)
Clone ID VSG224
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VSG224E
(Link to Original site)
Representative DNA sequence 
>VSG224 (VSG224Q) /CSM/VS/VSG2-A/VSG224Q.Seq.d/
CTGNTAAGTTCTCGAGAAGAAGACCTTCCGATTCCGCATCGAATAATGATGAGAAGTTTC
CATTAAAAAAGAATCATGCTACTCCAAATCATAACTCTCATAACAGGAATTCCTTTGATG
CACAAGCTGATAAGATTAGAGCTGCAATATTACCTGAAATAAGAAAAGACTCTAAGGTAG
ACCTCAAAAAGATAAGAAGCAGTTTCATCGTTTACCGTCAGAGCAAAGGCTACTGTCTCA
ATTGTGGTAAATCAAATCACTCCACATCTACATGTAGAATTGATCCGGTCGATGCCAAAG
CCAATCCAGGTCCATCAGCCAAAAAGCAACAATGACTCGATGAAGGTGATGAGTCCAAGT
CAAAGATTTTAAATAAAAATTATAAAATTTCACAATATCATCACCTTGAGGATAATGATT
ACCCAGTGAACAAAGATTCACTTTATACTCTTAACTCTTTTTATAATCAAAACATTGTTA
AACAGTTACATCAACAAAGCCAGCAACATGATGTTCATAAAAACATCAACAAACTTAAAG
AATACGTCAAAGACTCATATGGTATAAAACTAGTTCCTGATACAACCGTTCCAATTCCAA
AAGCTGTTGAATCAGAGATGAAGTATAAACCACAGAAGATTGAATTTAATACTCCAACAG
AGTTAACAAATGTTCAAGATAAAAACTANACTGTAAACATNTTCAATCGTCCAAAGATAT
TGAATTCTAGTAAAGAATTAATTAA
sequence update 2001. 3.25
Translated Amino Acid sequence 
xkfsrrrpsdsasnndekfplkknhatpnhnshnrnsfdaqadkiraailpeirkdskvd
lkkirssfivyrqskgyclncgksnhststcridpvdakanpgpsakkqq*LDEGDESKS
KILNKNYKISQYHHLEDNDYPVNKDSLYTLNSFYNQNIVKQLHQQSQQHDVHKNINKLKE
YVKDSYGIKLVPDTTVPIPKAVESEMKYKPQKIEFNTPTELTNVQDKNXTVNXFNRPKIL
NSSKELI
Translated Amino Acid sequence (All Frames) 
Frame A:
lxssreedlpiphrimmrsfh*krimllqiitlitgiplmhklirlelqyylk*ektlr*
tskr*eavssftvrakatvsivvnqitphlhvelirsmpkpiqvhqpksnndsmkvmsps
qrf*ikiikfhniitlrimitq*tkihfilltlfiiktllnsyinkasnmmfiktstnlk
ntskthmv*n*fliqpfqfqkllnqr*sinhrrlnlilqqs*qmfkiktxl*txsivqry
*ilvkn*l


Frame B:
x*vlekktfrfrie***evsikkescysks*ls*qefl*cts**d*scnit*nkkrl*gr
pqkdkkqfhrlpseqrllsqlw*ikslhiym*n*sgrcqsqsrsisqkatmtr*r**vqv
kdfk*kl*nftissp*g**lpseqrftlys*lfl*skhc*tvtstkpat*cs*khqqt*r
irqrliwyktss*ynrsnsksc*irdev*tted*i*ysnrvnkcsr*klxckhxqsskdi
ef**rin*


Frame C:
xkfsrrrpsdsasnndekfplkknhatpnhnshnrnsfdaqadkiraailpeirkdskvd
lkkirssfivyrqskgyclncgksnhststcridpvdakanpgpsakkqq*LDEGDESKS
KILNKNYKISQYHHLEDNDYPVNKDSLYTLNSFYNQNIVKQLHQQSQQHDVHKNINKLKE
YVKDSYGIKLVPDTTVPIPKAVESEMKYKPQKIEFNTPTELTNVQDKNXTVNXFNRPKIL
NSSKELI
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSG224 (VSG224Q) /CSM/VS/VSG2-A/VSG224Q.Seq.d/                       1459   0.0
AHH420 (AHH420Q) /CSM/AH/AHH4-A/AHH420Q.Seq.d/                       1168   0.0
AHN492 (AHN492Q) /CSM/AH/AHN4-D/AHN492Q.Seq.d/                       1162   0.0
VHM471 (VHM471Q) /CSM/VH/VHM4-C/VHM471Q.Seq.d/                       1130   0.0
AHF305 (AHF305Q) /CSM/AH/AHF3-A/AHF305Q.Seq.d/                       1063   0.0
AHG780 (AHG780Q) /CSM/AH/AHG7-D/AHG780Q.Seq.d/                        979   0.0
SFE733 (SFE733Q) /CSM/SF/SFE7-B/SFE733Q.Seq.d/                        940   0.0
VHK409 (VHK409Q) /CSM/VH/VHK4-A/VHK409Q.Seq.d/                        926   0.0
AHC708 (AHC708Q) /CSM/AH/AHC7-A/AHC708Q.Seq.d/                        854   0.0
VHG370 (VHG370Q) /CSM/VH/VHG3-C/VHG370Q.Seq.d/                        827   0.0
own update 2003. 1. 9
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF049230|AF049230.1 Dictyostelium discoideum LTR-retrotransposon Skipper, Gag (gag) gene, complete cds; and Pro (pro) and Pol (pol) genes, partial cds.  1459   0.0    1
AF017040|AF017040.1 Dictyostelium discoideum LTR-retrotransposon Skipper, Gag (gag) mRNA, complete cds; and Pro (pro) and Pol (pol) mRNA, partial cds.  1459   0.0    1
AC115612|AC115612.2 Dictyostelium discoideum chromosome 2 map 6245135-6357017 strain AX4, complete sequence.   371   1e-98  3
AC041029|AC041029.2 Homo sapiens chromosome 2 clone RP11-202B22 map 2, WORKING DRAFT SEQUENCE, 12 unordered pieces.    44   4e-04  2
AC109233|AC109233.10 Mus musculus chromosome 16 clone RP23-219N5 map 16, *** SEQUENCING IN PROGRESS ***, 2 ordered pieces.    56   8e-04  1
AZ088987|AZ088987.1 RPCI-23-26M13.TV RPCI-23 Mus musculus genomic clone RPCI-23-26M13, genomic survey sequence.    56   8e-04  1
AC105980|AC105980.5 Mus musculus chromosome 3 clone RP24-234E11 map 3, *** SEQUENCING IN PROGRESS ***.    56   8e-04  1
AC117080|AC117080.2 Dictyostelium discoideum chromosome 2 map complement(821514-735598) strain AX4, complete sequence.    42   0.005  6
AL445565|AL445565.1 Mycoplasma pulmonis (strain UAB CTIP) complete genome; segment 3/3.    36   0.017  11
AE014834|AE014834.1 Plasmodium falciparum 3D7 chromosome 10 section 6 of 7 of the complete sequence.    34   0.028  11
dna update 2004. 3.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF017040_2(AF017040|pid:none) Dictyostelium discoideum LTR-retro...   275   1e-72
AF017040_1(AF017040|pid:none) Dictyostelium discoideum LTR-retro...   226   5e-58
AL031745_47(AL031745|pid:none) Plasmodium falciparum DNA from MA...    40   0.094
AE014829_35(AE014829|pid:none) Plasmodium falciparum 3D7 chromos...    39   0.12
CP001019_1675(CP001019|pid:none) Coxiella burnetii CbuG_Q212, co...    38   0.27
AL844506_214(AL844506|pid:none) Plasmodium falciparum 3D7 chromo...    38   0.36
AP009049_1905(AP009049|pid:none) Clostridium kluyveri NBRC 12016...    38   0.36
AL844507_12(AL844507|pid:none) Plasmodium falciparum 3D7 chromos...    37   0.47
AE014819_52(AE014819|pid:none) Plasmodium falciparum 3D7 chromos...    37   0.79
AE013599_3336(AE013599|pid:none) Drosophila melanogaster chromos...    37   0.79
protein update 2009. 3.22
PSORT 

 psg: 0.75  gvh: 0.33  alm: 0.53  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for VSG224 is nuc
5' end seq. ID VSG224F
5' end seq. 
>VSG224F.Seq
CTGNTAAGTTCTCGAGAAGAAGACCTTCCGATTCCGCATCGAATAATGATGAGAAGTTTC
CATTAAAAAAGAATCATGCTACTCCAAATCATAACTCTCATAACAGGAATTCCTTTGATG
CACAAGCTGATAAGATTAGAGCTGCAATATTACCTGAAATAAGAAAAGACTCTAAGGTAG
ACCTCAAAAAGATAAGAAGCAGTTTCATCGTTTACCGTCAGAGCAAAGGCTACTGTCTCA
ATTGTGGTAAATCAAATCACTCCACATCTACATGTAGAATTGATCCGGTCGATGCCAAAG
CCAATCCAGGTCCATCAGCCAAAAAGCAACAATGACTCGATGAAGGTGATGAGTCCAAGT
CAAAGATTTTAAATAAAAATTATAAAATTTCACAATATCATCACCTTGAGGATAATGATT
ACCCAGTGAACAAAGATTCACTTTATACTCTTAACTCTTTTTATAATC----------
Length of 5' end seq. 468
3' end seq. ID VSG224Z
3' end seq. 
>VSG224Z.Seq
----------AAGAAAAGACTCTAAGGTAGACCTCAAAAAGATAAGAAGCAGTTTCATCG
TTTACCGTCAGAGCAAAGGCTACTGTCTCAATTGTGGTAAATCAAATCACTCCACATCTA
CATGTAGAATTGATCCGGTCGATGCCAAAGCCAATCCAGGTCCATCAGCCAAAAAGCAAC
AATGACTCGATGAAGGTGATGAGTCCAAGTCAAAGATTTTAAATAAAAATTATAAAATTT
CACAATATCATCACCTTGAGGATAATGATTACCCAGTGAACAAAGATTCACTTTATACTC
TTAACTCTTTTTATAATCAAAACATTGTTAAACAGTTACATCAACAAAGCCAGCAACATG
ATGTTCATAAAAACATCAACAAACTTAAAGAATACGTCAAAGACTCATATGGTATAAAAC
TAGTTCCTGATACAACCGTTCCAATTCCAAAAGCTGTTGAATCAGAGATGAAGTATAAAC
CACAGAAGATTGAATTTAATACTCCAACAGAGTTAACAAATGTTCAAGATAAAAACTANA
CTGTAAACATNTTCAATCGTCCAAAGATATTGAATTCTAGTAAAGAATTAATTAA
Length of 3' end seq. 585
Connected seq. ID VSG224P
Connected seq. 
>VSG224P.Seq
CTGNTAAGTTCTCGAGAAGAAGACCTTCCGATTCCGCATCGAATAATGATGAGAAGTTTC
CATTAAAAAAGAATCATGCTACTCCAAATCATAACTCTCATAACAGGAATTCCTTTGATG
CACAAGCTGATAAGATTAGAGCTGCAATATTACCTGAAATAAGAAAAGACTCTAAGGTAG
ACCTCAAAAAGATAAGAAGCAGTTTCATCGTTTACCGTCAGAGCAAAGGCTACTGTCTCA
ATTGTGGTAAATCAAATCACTCCACATCTACATGTAGAATTGATCCGGTCGATGCCAAAG
CCAATCCAGGTCCATCAGCCAAAAAGCAACAATGACTCGATGAAGGTGATGAGTCCAAGT
CAAAGATTTTAAATAAAAATTATAAAATTTCACAATATCATCACCTTGAGGATAATGATT
ACCCAGTGAACAAAGATTCACTTTATACTCTTAACTCTTTTTATAATC----------AA
GAAAAGACTCTAAGGTAGACCTCAAAAAGATAAGAAGCAGTTTCATCGTTTACCGTCAGA
GCAAAGGCTACTGTCTCAATTGTGGTAAATCAAATCACTCCACATCTACATGTAGAATTG
ATCCGGTCGATGCCAAAGCCAATCCAGGTCCATCAGCCAAAAAGCAACAATGACTCGATG
AAGGTGATGAGTCCAAGTCAAAGATTTTAAATAAAAATTATAAAATTTCACAATATCATC
ACCTTGAGGATAATGATTACCCAGTGAACAAAGATTCACTTTATACTCTTAACTCTTTTT
ATAATCAAAACATTGTTAAACAGTTACATCAACAAAGCCAGCAACATGATGTTCATAAAA
ACATCAACAAACTTAAAGAATACGTCAAAGACTCATATGGTATAAAACTAGTTCCTGATA
CAACCGTTCCAATTCCAAAAGCTGTTGAATCAGAGATGAAGTATAAACCACAGAAGATTG
AATTTAATACTCCAACAGAGTTAACAAATGTTCAAGATAAAAACTANACTGTAAACATNT
TCAATCGTCCAAAGATATTGAATTCTAGTAAAGAATTAATTAA
Length of connected seq. 1053
Full length Seq ID VSG224E
Full length Seq. 
>VSG224E.Seq
CTGNTAAGTTCTCGAGAAGAAGACCTTCCGATTCCGCATCGAATAATGATGAGAAGTTTC
CATTAAAAAAGAATCATGCTACTCCAAATCATAACTCTCATAACAGGAATTCCTTTGATG
CACAAGCTGATAAGATTAGAGCTGCAATATTACCTGAAATAAGAAAAGACTCTAAGGTAG
ACCTCAAAAAGATAAGAAGCAGTTTCATCGTTTACCGTCAGAGCAAAGGCTACTGTCTCA
ATTGTGGTAAATCAAATCACTCCACATCTACATGTAGAATTGATCCGGTCGATGCCAAAG
CCAATCCAGGTCCATCAGCCAAAAAGCAACAATGACTCGATGAAGGTGATGAGTCCAAGT
CAAAGATTTTAAATAAAAATTATAAAATTTCACAATATCATCACCTTGAGGATAATGATT
ACCCAGTGAACAAAGATTCACTTTATACTCTTAACTCTTTTTATAATCAAAACATTGTTA
AACAGTTACATCAACAAAGCCAGCAACATGATGTTCATAAAAACATCAACAAACTTAAAG
AATACGTCAAAGACTCATATGGTATAAAACTAGTTCCTGATACAACCGTTCCAATTCCAA
AAGCTGTTGAATCAGAGATGAAGTATAAACCACAGAAGATTGAATTTAATACTCCAACAG
AGTTAACAAATGTTCAAGATAAAAACTANACTGTAAACATNTTCAATCGTCCAAAGATAT
TGAATTCTAGTAAAGAATTAATTAA
Length of full length seq. 745