>VSG218 (VSG218Q) /CSM/VS/VSG2-A/VSG218Q.Seq.d/
GAGAATCGTTGACTCTGGTTTAACCTTAAAACATTTAATGGAATTATTGAATCATCGTAA
TCCAAATTCACTTCATACCGCCGTTTTACTTCGTAAAAAAGAAGGTCTCAAAGTTGAAGT
ACCAGTTAAATATATGGGCTTTGATATTCCAATGGTTTTCATCATTGGTTATGGTTTAGA
TTTTGCTGAAAATTATAGAGAATTACCATATTTAGGTGAATTAAAAGAAGAATGTTATAA
AAAATAAATAAATTTTAAAGAAAAAAAAAAXXXXXXXXXXGAAGATATCGTTGACTCTGG
TTTAACCTTAAAACATTTAATGGAATTATTGAATCATCGTAATCCAAATTCACTTCATAC
CGCCGTTTTACTTCGTAAAAAAGAAGGTCTCAAAGTTGAAGTACCAGTTAAATATATGGG
CTTTGATATTCCAATGGTTTTCATCATTGGTTATGGTTTAGATTTTGCTGAAAATTATAG
AGAATTACCAT

rivdsgltlkhlmellnhrnpnslhtavllrkkeglkvevpvkymgfdipmvfiigygld
faenyrelpylgelkeecykk*INFKEKK---

---EDIVDSGLTLKHLMELLNHRNPNSLHTAVLLRKKEGLKVEVPVKYMGFDIPMVFIIG
YGLDFAENYRELP

Frame A:
enr*lwfnlktfngiiess*skftsyrrfts*krrsqs*sts*iygl*ysngfhhwlwfr
fc*kl*ritifr*ikrrml*kinkf*rkkk---

---EDIVDSGLTLKHLMELLNHRNPNSLHTAVLLRKKEGLKVEVPVKYMGFDIPMVFIIG
YGLDFAENYRELP

Frame B:
rivdsgltlkhlmellnhrnpnslhtavllrkkeglkvevpvkymgfdipmvfiigygld
faenyrelpylgelkeecykk*INFKEKK---

---kisltlv*p*ni*wny*iiviqihfippfyfvkkkvsklkyqlniwalifqwfsslv
mv*illkiienyh

Frame C:
esltlv*p*ni*wny*iiviqihfippfyfvkkkvsklkyqlniwalifqwfsslvmv*i
llkiienyhi*vn*kknviknk*ilkkkk---

---ryr*lwfnlktfngiiess*skftsyrrfts*krrsqs*sts*iygl*ysngfhhwl
wfrfc*kl*rit

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSG218 (VSG218Q) /CSM/VS/VSG2-A/VSG218Q.Seq.d/                        515   e-145
SSJ877 (SSJ877Q) /CSM/SS/SSJ8-D/SSJ877Q.Seq.d/                        507   e-143
SSJ501 (SSJ501Q) /CSM/SS/SSJ5-A/SSJ501Q.Seq.d/                        507   e-143
SSI585 (SSI585Q) /CSM/SS/SSI5-D/SSI585Q.Seq.d/                        507   e-143
SFL108 (SFL108Q) /CSM/SF/SFL1-A/SFL108Q.Seq.d/                         40   0.031
AHB477 (AHB477Q) /CSM/AH/AHB4-D/AHB477Q.Seq.d/                         40   0.031
VHH573 (VHH573Q) /CSM/VH/VHH5-D/VHH573Q.Seq.d/                         38   0.12
VHH129 (VHH129Q) /CSM/VH/VHH1-B/VHH129Q.Seq.d/                         38   0.12
VFK587 (VFK587Q) /CSM/VF/VFK5-D/VFK587Q.Seq.d/                         38   0.12
SSM159 (SSM159Q) /CSM/SS/SSM1-C/SSM159Q.Seq.d/                         38   0.12

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC133957|AC133957.1 Mus musculus chromosome UNK clone RP23-367D8, WORKING DRAFT SEQUENCE, 2 unordered pieces.    40  0.006  7
BE475560|BE475560.1 sp79g03.y1 Gm-c1044 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1044-2189 5' similar to TR:O80629 O80629 PUTATIVE LYSOPHOSPHOLIPASE. ;, mRNA sequence.    52  0.007  1
AX344550|AX344550.1 Sequence 1 from Patent WO0200932.             34  0.026  5
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    42  0.054  6
AC137971|AC137971.4 Mus musculus, clone RP23-135B1, complete sequence.    40  0.084  4
AF242433|AF242433.1 Mus musculus delta Naip1 pseudogene, exons 7-9; and neuronal apoptosis inhibitory protein 7 (Naip7) gene, complete cds.    42  0.15  3
BH860705|BH860705.1 ORNL203 Poplar BAC Library Populus balsamifera subsp. trichocarpa genomic, DNA sequence.    40  0.21  3
AC124683|AC124683.3 Mus musculus chromosome 6 clone RP24-349E11, complete sequence.    44  0.25  5
AL614938|AL614938.1 T3 end of clone 010DG04 of library SmBAC1 from strain Puerto-Rican  of Schistosoma mansoni.    44  0.30  2
BH177981|BH177981.1 010_N_08-rev SmBAC1 Schistosoma mansoni genomic clone 010N08 5', DNA sequence.    44  0.30  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM412317_3532(AM412317|pid:none) Clostridium botulinum A str. AT...    98   8e-20
CP000962_3531(CP000962|pid:none) Clostridium botulinum A3 str. L...    98   8e-20
CP000431_4354(CP000431|pid:none) Rhodococcus jostii RHA1, comple...    94   2e-18
AE001437_3152(AE001437|pid:none) Clostridium acetobutylicum ATCC...    92   5e-18
CP000667_4252(CP000667|pid:none) Salinispora tropica CNB-440, co...    92   5e-18
CR931997_277(CR931997|pid:none) Corynebacterium jeikeium K411 co...    92   6e-18
CP000656_1398(CP000656|pid:none) Mycobacterium gilvum PYR-GCK, c...    92   8e-18
CP000384_4757(CP000384|pid:none) Mycobacterium sp. MCS, complete...    91   1e-17
CP000511_5297(CP000511|pid:none) Mycobacterium vanbaalenii PYR-1...    91   1e-17
CP001337_2578(CP001337|pid:none) Chloroflexus aggregans DSM 9485...    91   1e-17

 psg: 0.81  gvh: 0.34  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.15  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 48.0 %: nuclear
	  4.0 %: vesicles of secretory system

>> prediction for VSG218 is cyt

>VSG218F.Seq
GAGAATCGTTGACTCTGGTTTAACCTTAAAACATTTAATGGAATTATTGAATCATCGTAA
TCCAAATTCACTTCATACCGCCGTTTTACTTCGTAAAAAAGAAGGTCTCAAAGTTGAAGT
ACCAGTTAAATATATGGGCTTTGATATTCCAATGGTTTTCATCATTGGTTATGGTTTAGA
TTTTGCTGAAAATTATAGAGAATTACCATATTTAGGTGAATTAAAAGAAGAATGTTATAA
AAAATAAATAAATTTTAAAGAAAAAAAAAA----------

>VSG218Z.Seq
----------GAAGATATCGTTGACTCTGGTTTAACCTTAAAACATTTAATGGAATTATT
GAATCATCGTAATCCAAATTCACTTCATACCGCCGTTTTACTTCGTAAAAAAGAAGGTCT
CAAAGTTGAAGTACCAGTTAAATATATGGGCTTTGATATTCCAATGGTTTTCATCATTGG
TTATGGTTTAGATTTTGCTGAAAATTATAGAGAATTACCAT

>VSG218P.Seq
GAGAATCGTTGACTCTGGTTTAACCTTAAAACATTTAATGGAATTATTGAATCATCGTAA
TCCAAATTCACTTCATACCGCCGTTTTACTTCGTAAAAAAGAAGGTCTCAAAGTTGAAGT
ACCAGTTAAATATATGGGCTTTGATATTCCAATGGTTTTCATCATTGGTTATGGTTTAGA
TTTTGCTGAAAATTATAGAGAATTACCATATTTAGGTGAATTAAAAGAAGAATGTTATAA
AAAATAAATAAATTTTAAAGAAAAAAAAAA----------GAAGATATCGTTGACTCTGG
TTTAACCTTAAAACATTTAATGGAATTATTGAATCATCGTAATCCAAATTCACTTCATAC
CGCCGTTTTACTTCGTAAAAAAGAAGGTCTCAAAGTTGAAGTACCAGTTAAATATATGGG
CTTTGATATTCCAATGGTTTTCATCATTGGTTATGGTTTAGATTTTGCTGAAAATTATAG
AGAATTACCAT