>VSG208 (VSG208Q) /CSM/VS/VSG2-A/VSG208Q.Seq.d/
ANCGAGGTTATATAACAGTCGAGAAGAATTTAACAAAATGTCAGAAATCAAAGAAAAGAG
TTGTTACAAATGTAAAGAAGTTGGTCATATCTCACGTAATTGCCCAAAGAATCCAGAAGC
TGGTGATCGTGCTTGTTATGTTTGTAACGTTGTTGGTCATTTAAGCCGTGAATGTCCACA
AAACCCACAACCAACCTTCGAAAAGAAAGACCCAATTAAATGTTACCAATGTAATGGTTT
TGGTCATTTTGCTAGAGATTGTAGAAGAGGTAGAGATAACAAATGTTACAACTGTGGTGG
TTTAGGTCACATCTCCAAGGATTGTCCATCACCAAGCACCAGAGGTCAAGGGTCGTGATG
CTGCCAAATGTTACAAATGTAACCAACCAGGTCACATCGCCAAAGCTTGCCCAGAAAACC
AATCCGAAAATTAAATTTGAAAATTAATTATTTTCATATCATCATAATCATTCAATACGC
GATATGATGAATTTTTGTTTTTGTTTCTGTATAAAAACAXXXXXXXXXXAGCGAAGGTTA
TATAACAGTCGAGAAGAATTTAACAAAATGTCAGAAATCAAAGAAAAGAGTTGTTACAAA
TGTAAAGAAGTTGGTCATATCTCACGTAATTGCCCAAAGAATCCAGAAGCTGGTGATCGT
GCTTGTTATGTTTGTAACGTTGTTGGTCATTTAAGCCGTGAATGTCCACAAAACCCACAA
CCAACCTTCGAAAAGAAAGACCCAATTAAATGTTACCAATGTAATGGTTTTGGTCATTTT
GCTAGAGATTGTAGAAGAGGTAGAGATAACAAATGTTACAACTGTGGTGGTTTAGGTCAC
ATCTCCAAGGATTGTCCATCACCAAGCACCAGAGGTCAAGGTCGTGATGCTGCCAAATGT
TACAAATGTAACCAACCAGGTCACATCGCCAAAGCTTGCCCAGAAAACCAATCCGAAAAT
TAAATTTGAAAATTAATTATTTTCATATCATCATAATCATTCAATACGCGATATGATGAA
TTTTTGTTTTGTTTCTGTATAAAAACAATTGGTCAATTTAAATTTNATTGTATTTATTAA
TATCCCTCACCAAAATTTANAATTAAAAATAAAAAAAA

xevi*qsrri*qnvrnqrkellqm*rswsylt*lpkesrsw*scllcl*rcwsfkp*mst
kpttnlrkerpn*mlpm*wfwsfc*rl*kr*r*qmlqlwwfrshlqglsitkhqrsrvvm
lpnvtnvtnqvtspklaqktnpkikfen*LFSYHHNHSIRDMMNFCFCFCIKT---

---RRLYNSREEFNKMSEIKEKSCYKCKEVGHISRNCPKNPEAGDRACYVCNVVGHLSRE
CPQNPQPTFEKKDPIKCYQCNGFGHFARDCRRGRDNKCYNCGGLGHISKDCPSPSTRGQG
RDAAKCYKCNQPGHIAKACPENQSEN*i*kliifiss*sfntrydeflfcfciktigqfk
fxciy*ypspkfxiknkk

Frame A:
xevi*qsrri*qnvrnqrkellqm*rswsylt*lpkesrsw*scllcl*rcwsfkp*mst
kpttnlrkerpn*mlpm*wfwsfc*rl*kr*r*qmlqlwwfrshlqglsitkhqrsrvvm
lpnvtnvtnqvtspklaqktnpkikfen*LFSYHHNHSIRDMMNFCFCFCIKT---

---segyitveknltkcqkskkrvvtnvkklvishviaqriqklvivlvmfvtllvi*av
nvhkthnqpskrktqlnvtnvmvlvilleiveeveitnvttvvv*vtsprivhhqapevk
vvmlpnvtnvtnqvtspklaqktnpkikfen*lfsyhhnhsirdmmnfcfvsv*kqlvnl
nxivfiniphqnlxlkikk

Frame B:
xrlynsreefnkmseikekscykckevghisrncpknpeagdracyvcnvvghlsrecpq
npqptfekkdpikcyqcngfghfardcrrgrdnkcyncgglghiskdcpspstrgqgs*c
cqmlqm*ptrshrqslprkpirklnlkinyfhiiiiiqyai**ifvfvsv*k---

---akvi*qsrri*qnvrnqrkellqm*rswsylt*lpkesrsw*scllcl*rcwsfkp*
mstkpttnlrkerpn*mlpm*wfwsfc*rl*kr*r*qmlqlwwfrshlqglsitkhqrsr
s*ccqmlqm*ptrshrqslprkpirklnlkinyfhiiiiiqyai**ifvlflyknnwsi*
ixlyllisltkixn*k*kk

Frame C:
rgyitveknltkcqkskkrvvtnvkklvishviaqriqklvivlvmfvtllvi*avnvhk
thnqpskrktqlnvtnvmvlvilleiveeveitnvttvvv*vtsprivhhqapevkgrda
akcykcnqpghiakacpenqsen*i*kliifiss*sfntrydeflflflykn---

---RRLYNSREEFNKMSEIKEKSCYKCKEVGHISRNCPKNPEAGDRACYVCNVVGHLSRE
CPQNPQPTFEKKDPIKCYQCNGFGHFARDCRRGRDNKCYNCGGLGHISKDCPSPSTRGQG
RDAAKCYKCNQPGHIAKACPENQSEN*i*kliifiss*sfntrydeflfcfciktigqfk
fxciy*ypspkfxiknkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSG208 (VSG208Q) /CSM/VS/VSG2-A/VSG208Q.Seq.d/                       2093   0.0
VSI861 (VSI861Q) /CSM/VS/VSI8-C/VSI861Q.Seq.d/                       1084   0.0
VSE189 (VSE189Q) /CSM/VS/VSE1-D/VSE189Q.Seq.d/                       1021   0.0
VFF106 (VFF106Q) /CSM/VF/VFF1-A/VFF106Q.Seq.d/                        981   0.0
SLD529 (SLD529Q) /CSM/SL/SLD5-B/SLD529Q.Seq.d/                        846   0.0
VSG120 (VSG120Q) /CSM/VS/VSG1-A/VSG120Q.Seq.d/                         54   5e-06
VSH854 (VSH854Q) /CSM/VS/VSH8-C/VSH854Q.Seq.d/                         38   0.28
VSF252 (VSF252Q) /CSM/VS/VSF2-C/VSF252Q.Seq.d/                         38   0.28
VSE744 (VSE744Q) /CSM/VS/VSE7-B/VSE744Q.Seq.d/                         38   0.28
VSD845 (VSD845Q) /CSM/VS/VSD8-B/VSD845Q.Seq.d/                         38   0.28

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AP003244|AP003244.3 Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 1, PAC clone:P0419B01.    44  0.027  3
U88308|U88308.1 Caenorhabditis elegans cosmid C32E8.    36  0.028  4
CD268269|CD268269.1 tab82d02.x1 Hydra EST -III Hydra magnipapillata cDNA 3' similar to SW:HEXP_LEIMA Q04832 DNA-BINDING PROTEIN HEXBP ;, mRNA sequence.    36  0.043  3
BH959920|BH959920.1 odd60c06.g1 B.oleracea002 Brassica oleracea genomic, DNA sequence.    34  0.049  3
BZ014870|BZ014870.1 oeh50c09.b1 B.oleracea002 Brassica oleracea genomic, DNA sequence.    34  0.052  3
AL049911|AL049911.2 Homo sapiens chromosome 21 PAC RPCIP704C216Q2.    42  0.056  3
BX511259|BX511259.2 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-125E6.    34  0.094  8
AL424647|AL424647.1 T3 end of clone XAZ0AA001F04 of library XAZ0AA from strain CBS 712  of Kluyveromyces marxianus.    36  0.099  3
U10402|U10402.2 Caenorhabditis elegans cosmid C34E10, complete sequence.    42  0.10  3
CB936093|CB936093.1 taa33b08.y2 Hydra EST -III Hydra magnipapillata cDNA 5' similar to TR:Q9W6Q5 Q9W6Q5 CELLULAR NUCLEIC ACID BINDING PROTEIN. ;, mRNA sequence.    36  0.10  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270155_34(AM270155|pid:none) Aspergillus niger contig An07c038...   129   2e-28
AP007161_857(AP007161|pid:none) Aspergillus oryzae RIB40 genomic...   127   5e-28
AM920427_519(AM920427|pid:none) Penicillium chrysogenum Wisconsi...   127   9e-28
(Q04832) RecName: Full=DNA-binding protein HEXBP; AltName: Full=...   124   7e-27
CT005272_169(CT005272|pid:none) Leishmania major strain Friedlin...   121   4e-26
EF070485_1(EF070485|pid:none) Maconellicoccus hirsutus clone WHM...   120   8e-26
AM502254_417(AM502254|pid:none) Leishmania infantum chromosome 36.    120   8e-26
L03710_1(L03710|pid:none) Tetrahymena thermophila cnjB (cnjB) ge...   117   5e-25
CU640366_1371(CU640366|pid:none) Podospora anserina genomic DNA ...   117   9e-25
EF638949_1(EF638949|pid:none) Triatoma infestans clone TI-138 E3...   116   1e-24

 psg: 0.80  gvh: 0.39  alm: 0.52  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: mitochondrial
	 32.0 %: cytoplasmic
	 20.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum
	  4.0 %: peroxisomal

>> prediction for VSG208 is mit

>VSG208F.Seq
ANCGAGGTTATATAACAGTCGAGAAGAATTTAACAAAATGTCAGAAATCAAAGAAAAGAG
TTGTTACAAATGTAAAGAAGTTGGTCATATCTCACGTAATTGCCCAAAGAATCCAGAAGC
TGGTGATCGTGCTTGTTATGTTTGTAACGTTGTTGGTCATTTAAGCCGTGAATGTCCACA
AAACCCACAACCAACCTTCGAAAAGAAAGACCCAATTAAATGTTACCAATGTAATGGTTT
TGGTCATTTTGCTAGAGATTGTAGAAGAGGTAGAGATAACAAATGTTACAACTGTGGTGG
TTTAGGTCACATCTCCAAGGATTGTCCATCACCAAGCACCAGAGGTCAAGGGTCGTGATG
CTGCCAAATGTTACAAATGTAACCAACCAGGTCACATCGCCAAAGCTTGCCCAGAAAACC
AATCCGAAAATTAAATTTGAAAATTAATTATTTTCATATCATCATAATCATTCAATACGC
GATATGATGAATTTTTGTTTTTGTTTCTGTATAAAAACA----------

>VSG208Z.Seq
----------AGCGAAGGTTATATAACAGTCGAGAAGAATTTAACAAAATGTCAGAAATC
AAAGAAAAGAGTTGTTACAAATGTAAAGAAGTTGGTCATATCTCACGTAATTGCCCAAAG
AATCCAGAAGCTGGTGATCGTGCTTGTTATGTTTGTAACGTTGTTGGTCATTTAAGCCGT
GAATGTCCACAAAACCCACAACCAACCTTCGAAAAGAAAGACCCAATTAAATGTTACCAA
TGTAATGGTTTTGGTCATTTTGCTAGAGATTGTAGAAGAGGTAGAGATAACAAATGTTAC
AACTGTGGTGGTTTAGGTCACATCTCCAAGGATTGTCCATCACCAAGCACCAGAGGTCAA
GGTCGTGATGCTGCCAAATGTTACAAATGTAACCAACCAGGTCACATCGCCAAAGCTTGC
CCAGAAAACCAATCCGAAAATTAAATTTGAAAATTAATTATTTTCATATCATCATAATCA
TTCAATACGCGATATGATGAATTTTTGTTTTGTTTCTGTATAAAAACAATTGGTCAATTT
AAATTTNATTGTATTTATTAATATCCCTCACCAAAATTTANAATTAAAAATAAAAAAAA

>VSG208P.Seq
ANCGAGGTTATATAACAGTCGAGAAGAATTTAACAAAATGTCAGAAATCAAAGAAAAGAG
TTGTTACAAATGTAAAGAAGTTGGTCATATCTCACGTAATTGCCCAAAGAATCCAGAAGC
TGGTGATCGTGCTTGTTATGTTTGTAACGTTGTTGGTCATTTAAGCCGTGAATGTCCACA
AAACCCACAACCAACCTTCGAAAAGAAAGACCCAATTAAATGTTACCAATGTAATGGTTT
TGGTCATTTTGCTAGAGATTGTAGAAGAGGTAGAGATAACAAATGTTACAACTGTGGTGG
TTTAGGTCACATCTCCAAGGATTGTCCATCACCAAGCACCAGAGGTCAAGGGTCGTGATG
CTGCCAAATGTTACAAATGTAACCAACCAGGTCACATCGCCAAAGCTTGCCCAGAAAACC
AATCCGAAAATTAAATTTGAAAATTAATTATTTTCATATCATCATAATCATTCAATACGC
GATATGATGAATTTTTGTTTTTGTTTCTGTATAAAAACA----------AGCGAAGGTTA
TATAACAGTCGAGAAGAATTTAACAAAATGTCAGAAATCAAAGAAAAGAGTTGTTACAAA
TGTAAAGAAGTTGGTCATATCTCACGTAATTGCCCAAAGAATCCAGAAGCTGGTGATCGT
GCTTGTTATGTTTGTAACGTTGTTGGTCATTTAAGCCGTGAATGTCCACAAAACCCACAA
CCAACCTTCGAAAAGAAAGACCCAATTAAATGTTACCAATGTAATGGTTTTGGTCATTTT
GCTAGAGATTGTAGAAGAGGTAGAGATAACAAATGTTACAACTGTGGTGGTTTAGGTCAC
ATCTCCAAGGATTGTCCATCACCAAGCACCAGAGGTCAAGGTCGTGATGCTGCCAAATGT
TACAAATGTAACCAACCAGGTCACATCGCCAAAGCTTGCCCAGAAAACCAATCCGAAAAT
TAAATTTGAAAATTAATTATTTTCATATCATCATAATCATTCAATACGCGATATGATGAA
TTTTTGTTTTGTTTCTGTATAAAAACAATTGGTCAATTTAAATTTNATTGTATTTATTAA
TATCCCTCACCAAAATTTANAATTAAAAATAAAAAAAA