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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSG174
Library VS
(Link to library)
Clone ID VSG174
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13926-1
Original site URL
Representative seq. ID VSG174P
(Link to Original site)
Representative DNA sequence 
>VSG174 (VSG174Q) /CSM/VS/VSG1-D/VSG174Q.Seq.d/
CAAATAAAATGAGATTATTATTAGCCCTCTTTTTCGTTCTTGCTTTAGTTTCCCCATCTT
TCACCCAAGTTTGGTCAAATTGTGGTACTGCTGCCGATAAATTCCAAATTACCAATGTAG
TATTAGACCCACCAACACCAGTTAAAGGACAAGATATCACAATCAGTGCATCAGGTATTC
TCGATGAAACCGTCACTGGTGGAAATGTTGCCGTTAAAGTCAAATATGGTTTCATTACAC
TTATCAATGAAAATGTTTCTATTTGCAGTTCTCAAGACCCATTAGCATGCCCAATTGCTG
CTGGTGATTACCAAAAGAATATGACCGAAATGATTCCATCTGATGCTCCATCAGGTAAAT
ACACTGGTAACGTTGTTATCACCGATCAAAACAATGCTGAAATCGCCTGTATTGATGTTG
ATATTAACTTGTAAACGAATAAAATAAAATAAAATAAAATAAAAACACACCATCGATAAA
TTATTTATAAATTAAAAAAAAAAXXXXXXXXXXCAAATAAAATGAGATTATTATTAGCCC
TCTTTTTCGTTCTTGCTTTAGTTTCCCCATCTTTCACCCAAGTTTGGTCAAATTGTGGTA
CTGCTGCCGATAAATTCCAAATTACCAATGTAGTATTAGACCCACCAACACCAGTTAAAG
GACAAGATATCACAATCAGTGCATCAGGTATTCTCGATGAAACCGTCACTGGTGGAAATG
TTGCCGTTAAAGTCAAATATGGTTTCATTACACTTATCAATGAAAATGTTTCTATTTGCA
GTTCTCAAGACCCATTAGCATGCCCAATTGCTGCTGGTGATTACCAAAAGAATATGACCG
AAATGATTCCATCTGATGCTCCATCAGGTAAATACACTGGTAACGTTGTTATCACCGATC
AAAACAATGCTGAAATCGCCTGTATTGCATGTTGATATTAACTTGTAAACGTAATAAAAT
AAAATAAAATAAAATAAAAACACACC
sequence update 2001. 3.22
Translated Amino Acid sequence 
nkmrlllalffvlalvspsftqvwsncgtaadkfqitnvvldpptpvkgqditisasgil
detvtggnvavkvkygfitlinenvsicssqdplacpiaagdyqknmtemipsdapsgky
tgnvvitdqnnaeiacidvdinl*tnkik*nkikthhr*IIYKLKKK---

---NKMRLLLALFFVLALVSPSFTQVWSNCGTAADKFQITNVVLDPPTPVKGQDITISAS
GILDETVTGGNVAVKVKYGFITLINENVSICSSQDPLACPIAAGDYQKNMTEMIPSDAPS
GKYTGNVVITDQNNAEIACIAC*y*lvnvik*nkik*kht
Translated Amino Acid sequence (All Frames) 
Frame A:
qik*dyy*psfsfll*fphlspkfgqivvllpinsklpm*y*thqhqlkdkisqsvhqvf
smkpslvemlplksnmvslhlsmkmflfavlkth*haqlllvitkri*pk*fhlmlhqvn
tlvtllspiktmlkspvlmliltckrik*nkik*khtidklfin*kk---

---qik*dyy*psfsfll*fphlspkfgqivvllpinsklpm*y*thqhqlkdkisqsvh
qvfsmkpslvemlplksnmvslhlsmkmflfavlkth*haqlllvitkri*pk*fhlmlh
qvntlvtllspiktmlkspvlhvdinl*t**nkik*nknt

Frame B:
k*neiiisplfrscfsfpifhpslvklwyccr*ipnyqcsirptnts*rtryhnqcirys
r*nrhwwkccr*sqiwfhytyq*kcfylqfsrpismpnccw*lpkeydrndsi*csir*i
hw*rcyhrskqc*nrly*c*y*lvne*nkik*nkntpsinyl*ikkk---

---k*neiiisplfrscfsfpifhpslvklwyccr*ipnyqcsirptnts*rtryhnqci
rysr*nrhwwkccr*sqiwfhytyq*kcfylqfsrpismpnccw*lpkeydrndsi*csi
r*ihw*rcyhrskqc*nrlycmliltckrnkik*nkikth

Frame C:
nkmrlllalffvlalvspsftqvwsncgtaadkfqitnvvldpptpvkgqditisasgil
detvtggnvavkvkygfitlinenvsicssqdplacpiaagdyqknmtemipsdapsgky
tgnvvitdqnnaeiacidvdinl*tnkik*nkikthhr*IIYKLKKK---

---NKMRLLLALFFVLALVSPSFTQVWSNCGTAADKFQITNVVLDPPTPVKGQDITISAS
GILDETVTGGNVAVKVKYGFITLINENVSICSSQDPLACPIAAGDYQKNMTEMIPSDAPS
GKYTGNVVITDQNNAEIACIAC*y*lvnvik*nkik*kht
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSG174 (VSG174Q) /CSM/VS/VSG1-D/VSG174Q.Seq.d/                        868   0.0
VSJ851 (VSJ851Q) /CSM/VS/VSJ8-C/VSJ851Q.Seq.d/                        866   0.0
VSJ841 (VSJ841Q) /CSM/VS/VSJ8-B/VSJ841Q.Seq.d/                        866   0.0
VSJ452 (VSJ452Q) /CSM/VS/VSJ4-C/VSJ452Q.Seq.d/                        866   0.0
VSI417 (VSI417Q) /CSM/VS/VSI4-A/VSI417Q.Seq.d/                        866   0.0
VSI354 (VSI354Q) /CSM/VS/VSI3-C/VSI354Q.Seq.d/                        866   0.0
VSG156 (VSG156Q) /CSM/VS/VSG1-C/VSG156Q.Seq.d/                        866   0.0
VSA770 (VSA770Q) /CSM/VS/VSA7-C/VSA770Q.Seq.d/                        866   0.0
VHF348 (VHF348Q) /CSM/VH/VHF3-B/VHF348Q.Seq.d/                        866   0.0
VSK373 (VSK373Q) /CSM/VS/VSK3-D/VSK373Q.Seq.d/                        862   0.0
own update 2003.10.28
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AX405179|AX405179.1 Sequence 3 from Patent WO0229023.             40  1e-04  4
BX160259|BX160259.1 Danio rerio genomic clone DKEY-146C24, genomic survey sequence.    46  0.83  1
AC115612|AC115612.2 Dictyostelium discoideum chromosome 2 map 6245135-6357017 strain AX4, complete sequence.    32  1.4  5
AC126782|AC126782.12 Medicago truncatula clone mth2-14p11, WORKING DRAFT SEQUENCE, 36 unordered pieces.    44  3.3  1
AQ261015|AQ261015.1 CITBI-E1-2515P5.TR CITBI-E1 Homo sapiens genomic clone 2515P5, DNA sequence.    34  9.3  2
AC120833|AC120833.4 Rattus norvegicus clone CH230-91L4, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    42     13  1
CC016838|CC016838.1 PUGJW25TB ZM_0.6_1.0_KB Zea mays genomic clone ZMMBTa401E01, DNA sequence.    42     13  1
CC918405|CC918405.1 t006c17ba.f1 TAMBT Bos taurus genomic clone t006c17ba, genomic survey sequence.    42     13  1
AC105571|AC105571.4 Rattus norvegicus clone CH230-232I12, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    42     13  1
AC141863|AC141863.6 Medicago truncatula clone mth2-14b10, WORKING DRAFT SEQUENCE, 1 ordered piece.    44     77  3
dna update 2003. 8.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q4P580) RecName: Full=Phosphatidylglycerol/phosphatidylinositol...    84   5e-15
(Q7RZ85) RecName: Full=Phosphatidylglycerol/phosphatidylinositol...    72   2e-11
(Q4X136) RecName: Full=Phosphatidylglycerol/phosphatidylinositol...    69   2e-10
(Q6CNE0) RecName: Full=Phosphatidylglycerol/phosphatidylinositol...    69   2e-10
AK227594_1(AK227594|pid:none) Arabidopsis thaliana mRNA for hypo...    69   3e-10
CP000502_275(CP000502|pid:none) Pichia stipitis CBS 6054 chromos...    67   7e-10
(Q12408) RecName: Full=Phosphatidylglycerol/phosphatidylinositol...    66   2e-09
AM920430_43(AM920430|pid:none) Penicillium chrysogenum Wisconsin...    65   3e-09
(Q6BV42) RecName: Full=Phosphatidylglycerol/phosphatidylinositol...    65   4e-09
(Q5KIR9) RecName: Full=Phosphatidylglycerol/phosphatidylinositol...    64   6e-09
protein update 2009. 3.22
PSORT 

 psg: 0.83  gvh: 0.88  alm: 0.24  top: 0.00  tms: 0.07  mit: 0.39  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.38  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: cytoplasmic
	 20.0 %: Golgi
	 20.0 %: endoplasmic reticulum
	 16.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for VSG174 is cyt
5' end seq. ID VSG174F
5' end seq. 
>VSG174F.Seq
CAAATAAAATGAGATTATTATTAGCCCTCTTTTTCGTTCTTGCTTTAGTTTCCCCATCTT
TCACCCAAGTTTGGTCAAATTGTGGTACTGCTGCCGATAAATTCCAAATTACCAATGTAG
TATTAGACCCACCAACACCAGTTAAAGGACAAGATATCACAATCAGTGCATCAGGTATTC
TCGATGAAACCGTCACTGGTGGAAATGTTGCCGTTAAAGTCAAATATGGTTTCATTACAC
TTATCAATGAAAATGTTTCTATTTGCAGTTCTCAAGACCCATTAGCATGCCCAATTGCTG
CTGGTGATTACCAAAAGAATATGACCGAAATGATTCCATCTGATGCTCCATCAGGTAAAT
ACACTGGTAACGTTGTTATCACCGATCAAAACAATGCTGAAATCGCCTGTATTGATGTTG
ATATTAACTTGTAAACGAATAAAATAAAATAAAATAAAATAAAAACACACCATCGATAAA
TTATTTATAAATTAAAAAAAAAA----------
Length of 5' end seq. 503
3' end seq. ID VSG174Z
3' end seq. 
>VSG174Z.Seq
----------CAAATAAAATGAGATTATTATTAGCCCTCTTTTTCGTTCTTGCTTTAGTT
TCCCCATCTTTCACCCAAGTTTGGTCAAATTGTGGTACTGCTGCCGATAAATTCCAAATT
ACCAATGTAGTATTAGACCCACCAACACCAGTTAAAGGACAAGATATCACAATCAGTGCA
TCAGGTATTCTCGATGAAACCGTCACTGGTGGAAATGTTGCCGTTAAAGTCAAATATGGT
TTCATTACACTTATCAATGAAAATGTTTCTATTTGCAGTTCTCAAGACCCATTAGCATGC
CCAATTGCTGCTGGTGATTACCAAAAGAATATGACCGAAATGATTCCATCTGATGCTCCA
TCAGGTAAATACACTGGTAACGTTGTTATCACCGATCAAAACAATGCTGAAATCGCCTGT
ATTGCATGTTGATATTAACTTGTAAACGTAATAAAATAAAATAAAATAAAATAAAAACAC
ACC
Length of 3' end seq. 473
Connected seq. ID VSG174P
Connected seq. 
>VSG174P.Seq
CAAATAAAATGAGATTATTATTAGCCCTCTTTTTCGTTCTTGCTTTAGTTTCCCCATCTT
TCACCCAAGTTTGGTCAAATTGTGGTACTGCTGCCGATAAATTCCAAATTACCAATGTAG
TATTAGACCCACCAACACCAGTTAAAGGACAAGATATCACAATCAGTGCATCAGGTATTC
TCGATGAAACCGTCACTGGTGGAAATGTTGCCGTTAAAGTCAAATATGGTTTCATTACAC
TTATCAATGAAAATGTTTCTATTTGCAGTTCTCAAGACCCATTAGCATGCCCAATTGCTG
CTGGTGATTACCAAAAGAATATGACCGAAATGATTCCATCTGATGCTCCATCAGGTAAAT
ACACTGGTAACGTTGTTATCACCGATCAAAACAATGCTGAAATCGCCTGTATTGATGTTG
ATATTAACTTGTAAACGAATAAAATAAAATAAAATAAAATAAAAACACACCATCGATAAA
TTATTTATAAATTAAAAAAAAAA----------CAAATAAAATGAGATTATTATTAGCCC
TCTTTTTCGTTCTTGCTTTAGTTTCCCCATCTTTCACCCAAGTTTGGTCAAATTGTGGTA
CTGCTGCCGATAAATTCCAAATTACCAATGTAGTATTAGACCCACCAACACCAGTTAAAG
GACAAGATATCACAATCAGTGCATCAGGTATTCTCGATGAAACCGTCACTGGTGGAAATG
TTGCCGTTAAAGTCAAATATGGTTTCATTACACTTATCAATGAAAATGTTTCTATTTGCA
GTTCTCAAGACCCATTAGCATGCCCAATTGCTGCTGGTGATTACCAAAAGAATATGACCG
AAATGATTCCATCTGATGCTCCATCAGGTAAATACACTGGTAACGTTGTTATCACCGATC
AAAACAATGCTGAAATCGCCTGTATTGCATGTTGATATTAACTTGTAAACGTAATAAAAT
AAAATAAAATAAAATAAAAACACACC
Length of connected seq. 976
Full length Seq ID -
Full length Seq. -
Length of full length seq. -