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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSG164
Library VS
(Link to library)
Clone ID VSG164
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15350-1
Original site URL
Representative seq. ID VSG164E
(Link to Original site)
Representative DNA sequence 
>VSG164 (VSG164Q) /CSM/VS/VSG1-C/VSG164Q.Seq.d/
GAAGTTAAAATGGCAGAAAAAGAACAAATTAAATTAGTCTTAGTTGGTGATGGTGGTGTT
GGTAAAACCACTTTTGTCCAACGTCATCTTACTGGTGAATTCGAACCAAGATATATCCCA
ACTCTTGTGTATCAGTCCATCCATTAATCTTCTACACCAATTTCGGTAAAATCCACTTCA
ATGTATGGGATACCGCTGGTCAAGAAAAATTCGGTGGTTTAANAGATGGTTATTATATTC
AAGGTAATTGTGCCATTATTATGTTTGATGTCACATCCCGTATTTCATACAAAAATGTTC
CAAATTGGCACTCTGATTTAACCAGAGTTTGTGAAAACATCCCAATCGTTTTATGTGGTA
ACAAAGTTGATGTTAAAGATCGTAAAGTCAAACCATCCCAAATTGTTTTCCATCGTAGAT
ACAATTTATCATACTATGATGTTTTCAGCCAAATCAAATTACAATTTCGAAAAACCATTN
GTNTGGTTAACATCAAAACNCCNCGGNAACAAGGCTGTCACTCTCGTTCAACAACCAACC
TTAAAATTACCAGAAACCGTTCTCGATTCCNACTTAATGAGTTTANATGAAAAANAAGTC
GCTGATGCTGCTGCCCNCCCNTTACCAGAAGACAATGATGACNTGTAAACNAATTTAAAA
AAAAAAAACAATAAAAAAAAATAATAATAATAATAATAAAGCCAACAATAAATTTCAATA
sequence update 2001. 3.25
Translated Amino Acid sequence 
s*ngrkrtn*islsw*wwcw*nhfcptssyw*IRTKIYPNSCVSVHPLIFYTNFGKIHFN
VWDTAGQEKFGGLXDGYYIQGNCAIIMFDVTSRISYKNVPNWHSDLTRVCENIPIVLCGN
KVDVKDRKVKPSQIVFHRRYNLSYYDVFSQIKLQFRKTIXXVNIKTPRXQGCHSRSTTNL
KITRNRSRFXLNEFX*kxsr*cccppxtrrq**xvnxfkkkkq*kkiiiiiikptinfn
Translated Amino Acid sequence (All Frames) 
Frame A:
evkmaekeqiklvlvgdggvgkttfvqrhltgefepryiptlvyqsih*sstpisvksts
mygiplvkknsvv*xmviifkvivpllclmshpvfhtkmfqigtli*pefvktsqsfyvv
tklmlkivksnhpklfsivdtiyhtmmfsaksnynfekpxvwltskxxgnkavtlvqqpt
lklpetvldsxlmslxekxvadaaaxplpednddx*tnlkkknnkkk******sqq*isi


Frame B:
klkwqkknkln*s*lvmvvlvkpllsnvillvnsnqdisqllcispsinllhqfr*nplq
cmgyrwsrkirwfxrwllysr*lchyyv*chipyfiqkcsklal*fnqsl*khpnrfmw*
qs*c*rs*sqtipncfps*iqfiil*cfqpnqitisknhxxg*hqnxxxtrlslsfnnqp
*nyqkpfsipt**vxmkxkslmllpxxyqktmmtckxi*kkktikknnnnnnkannkfq


Frame C:
s*ngrkrtn*islsw*wwcw*nhfcptssyw*IRTKIYPNSCVSVHPLIFYTNFGKIHFN
VWDTAGQEKFGGLXDGYYIQGNCAIIMFDVTSRISYKNVPNWHSDLTRVCENIPIVLCGN
KVDVKDRKVKPSQIVFHRRYNLSYYDVFSQIKLQFRKTIXXVNIKTPRXQGCHSRSTTNL
KITRNRSRFXLNEFX*kxsr*cccppxtrrq**xvnxfkkkkq*kkiiiiiikptinfn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSG164 (VSG164Q) /CSM/VS/VSG1-C/VSG164Q.Seq.d/                       1181   0.0
SSI774 (SSI774Q) /CSM/SS/SSI7-D/SSI774Q.Seq.d/                       1150   0.0
SLA506 (SLA506Q) /CSM/SL/SLA5-A/SLA506Q.Seq.d/                       1148   0.0
VSD229 (VSD229Q) /CSM/VS/VSD2-B/VSD229Q.Seq.d/                       1136   0.0
VSG423 (VSG423Q) /CSM/VS/VSG4-A/VSG423Q.Seq.d/                       1118   0.0
VSI212 (VSI212Q) /CSM/VS/VSI2-A/VSI212Q.Seq.d/                       1114   0.0
AHG822 (AHG822Q) /CSM/AH/AHG8-A/AHG822Q.Seq.d/                       1114   0.0
VSI638 (VSI638Q) /CSM/VS/VSI6-B/VSI638Q.Seq.d/                       1086   0.0
VSF607 (VSF607Q) /CSM/VS/VSF6-A/VSF607Q.Seq.d/                       1031   0.0
VSG244 (VSG244Q) /CSM/VS/VSG2-B/VSG244Q.Seq.d/                       1021   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

L09720|L09720.1 Dictyostelium discoideum GTP-binding protein (TC4/RAN) mRNA, complete cds.   624   0.0    4
AX489114|AX489114.1 Sequence 6414 from Patent WO02053728.         115   5e-53  4
AF190700|AF190700.1 Candida albicans small G-protein Gsp1p (GSP1) gene, complete cds.   115   5e-51  3
CA758831|AAF60289.1, mRNA sequence.    74   1e-50  5
BM275423|BM275423.1 PfESToaa68f04.y1 Plasmodium falciparum 3D7 gametocyte cDNA library Plasmodium falciparum 3D7 cDNA 5' similar to SW:RAN_PLAFA P38545 GTP-BINDING NUCLEAR PROTEIN RAN/TC4. ;, mRNA sequence.    86   1e-35  4
BI815073|BI815073.1 PfESToaa14a04.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to SW:RAN_PLAFA P38545 GTP-BINDING NUCLEAR PROTEIN RAN/TC4. ;, mRNA sequence.    86   1e-35  4
BU498261|BU498261.1 PfESToab94h09.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to SW:RAN_PLAFA P38545 GTP-BINDING NUCLEAR PROTEIN RAN/TC4. ;, mRNA sequence.    86   1e-35  4
BM273404|BM273404.1 PfESToaa50c11.y1 Plasmodium falciparum 3D7 gametocyte cDNA library Plasmodium falciparum 3D7 cDNA 5' similar to SW:RAN_PLAFA P38545 GTP-BINDING NUCLEAR PROTEIN RAN/TC4. ;, mRNA sequence.    86   1e-35  4
BI815642|BI815642.1 PfESToaa31a10.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to SW:RAN_PLAFA P38545 GTP-BINDING NUCLEAR PROTEIN RAN/TC4. ;, mRNA sequence.    86   1e-35  4
BI815554|BI815554.1 PfESToaa29h11.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to SW:RAN_PLAFA P38545 GTP-BINDING NUCLEAR PROTEIN RAN/TC4. ;, mRNA sequence.    86   1e-35  4
dna update 2004. 3.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P33519) RecName: Full=GTP-binding nuclear protein Ran; AltName:...   228   9e-70
DQ090494_1(DQ090494|pid:none) Marsupenaeus japonicus Ras-related...   187   3e-58
JC1455(JC1455;S20712)GTP-binding protein Ran/TC4 - dog                192   5e-54
AJ608268_1(AJ608268|pid:none) Chironomus tentans ran1 mRNA for G...   193   4e-53
(O17915) RecName: Full=GTP-binding nuclear protein ran-1; AltNam...   191   1e-52
(Q7ZZX9) RecName: Full=GTP-binding nuclear protein Ran; AltName:...   189   1e-52
BC004272_1(BC004272|pid:none) Homo sapiens RAN, member RAS oncog...   191   2e-52
AY890939_1(AY890939|pid:none) Synthetic construct Homo sapiens c...   191   2e-52
(P42558) RecName: Full=GTP-binding nuclear protein Ran; AltName:...   191   2e-52
BC041293_1(BC041293|pid:none) Xenopus laevis RAN, member RAS onc...   191   2e-52
protein update 2009. 3.22
PSORT 

 psg: 0.88  gvh: 0.29  alm: 0.48  top: 0.47  tms: 0.00  mit: 0.33  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 28.0 %: cytoplasmic
	 20.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for VSG164 is nuc
5' end seq. ID VSG164F
5' end seq. 
>VSG164F.Seq
GAAGTTAAAATGGCAGAAAAAGAACAAATTAAATTAGTCTTAGTTGGTGATGGTGGTGTT
GGTAAAACCACTTTTGTCCAACGTCATCTTACTGGTGAATTCGAACCAAGATATATCCCA
ACTCTTGTGTATCAGTCCATCCATTAATCTTCTACACCAATTTCGGTAAAATCCACTTCA
ATGTATGGGATACCGCTGGTCAAGAAAAATTCGGTGGTTTAAGAGATGGTTATTATATTC
AAGGTAATTGTGCCATTATTATGTTTGATGTCACATCCCGTATTTCATACAAAAATGTTC
CAAATTGGCACTCTGATTTAACCAGAGTTTGTGAAAACATCCCAATCGTTTTATGTGGTA
ACAAAGTTGATGTTAAAGATCGTAAAGTCAAACCATCCCAAATTGTTTTCCATCGTAGAT
ACAATTTATCATACTATGATGTTTTCAGCCAAATCAAATTACAATTTCGAAA--------
--
Length of 5' end seq. 472
3' end seq. ID VSG164Z
3' end seq. 
>VSG164Z.Seq
----------GGTAAAATCCACTTCAATGTATGGGANACCNCTGGTCAANAAAAATTCGG
TGGTTTAAAAGATGGTTATTATATTCAAGGTAATTGNGCCATTATTATGTTTGATGTCAC
ATCCCGTATTTCANACAAAAATGTTCCAAATTGGCNCTNTGATTTAACCANAGTTTGTGA
AAACATCCCAATNGTTTTATGTGGTAACAAAGTTNATGTTAAANATNGTAAAGTCAAACC
ATCCCAAATTGTTTTCCATNGTANANACAATTTATCANACTATGATGTTTCAGCCAAATC
AAATTACAATTTNGAAAAACCATTNGTNTGGTTAACATCAAAACNCCNCGGNAACAAGGC
TGTCACTCTCGTTCAACAACCAACCTTAAAATTACCAGAAACCGTTCTCGATTCCNACTT
AATGAGTTTANATGAAAAANAAGTCGCTGATGCTGCTGCCCNCCCNTTACCAGAAGACAA
TGATGACNTGTAAACNAATTTAAAAAAAAAAAACAATAAAAAAAAATAATAATAATAATA
ATAAAGCCAACAATAAATTTCAATA
Length of 3' end seq. 555
Connected seq. ID VSG164P
Connected seq. 
>VSG164P.Seq
GAAGTTAAAATGGCAGAAAAAGAACAAATTAAATTAGTCTTAGTTGGTGATGGTGGTGTT
GGTAAAACCACTTTTGTCCAACGTCATCTTACTGGTGAATTCGAACCAAGATATATCCCA
ACTCTTGTGTATCAGTCCATCCATTAATCTTCTACACCAATTTCGGTAAAATCCACTTCA
ATGTATGGGATACCGCTGGTCAAGAAAAATTCGGTGGTTTAAGAGATGGTTATTATATTC
AAGGTAATTGTGCCATTATTATGTTTGATGTCACATCCCGTATTTCATACAAAAATGTTC
CAAATTGGCACTCTGATTTAACCAGAGTTTGTGAAAACATCCCAATCGTTTTATGTGGTA
ACAAAGTTGATGTTAAAGATCGTAAAGTCAAACCATCCCAAATTGTTTTCCATCGTAGAT
ACAATTTATCATACTATGATGTTTTCAGCCAAATCAAATTACAATTTCGAAA--------
--GGTAAAATCCACTTCAATGTATGGGANACCNCTGGTCAANAAAAATTCGGTGGTTTAA
AAGATGGTTATTATATTCAAGGTAATTGNGCCATTATTATGTTTGATGTCACATCCCGTA
TTTCANACAAAAATGTTCCAAATTGGCNCTNTGATTTAACCANAGTTTGTGAAAACATCC
CAATNGTTTTATGTGGTAACAAAGTTNATGTTAAANATNGTAAAGTCAAACCATCCCAAA
TTGTTTTCCATNGTANANACAATTTATCANACTATGATGTTTCAGCCAAATCAAATTACA
ATTTNGAAAAACCATTNGTNTGGTTAACATCAAAACNCCNCGGNAACAAGGCTGTCACTC
TCGTTCAACAACCAACCTTAAAATTACCAGAAACCGTTCTCGATTCCNACTTAATGAGTT
TANATGAAAAANAAGTCGCTGATGCTGCTGCCCNCCCNTTACCAGAAGACAATGATGACN
TGTAAACNAATTTAAAAAAAAAAAACAATAAAAAAAAATAATAATAATAATAATAAAGCC
AACAATAAATTTCAATA
Length of connected seq. 1027
Full length Seq ID VSG164E
Full length Seq. 
>VSG164E.Seq
GAAGTTAAAATGGCAGAAAAAGAACAAATTAAATTAGTCTTAGTTGGTGATGGTGGTGTT
GGTAAAACCACTTTTGTCCAACGTCATCTTACTGGTGAATTCGAACCAAGATATATCCCA
ACTCTTGTGTATCAGTCCATCCATTAATCTTCTACACCAATTTCGGTAAAATCCACTTCA
ATGTATGGGATACCGCTGGTCAAGAAAAATTCGGTGGTTTAANAGATGGTTATTATATTC
AAGGTAATTGTGCCATTATTATGTTTGATGTCACATCCCGTATTTCATACAAAAATGTTC
CAAATTGGCACTCTGATTTAACCAGAGTTTGTGAAAACATCCCAATCGTTTTATGTGGTA
ACAAAGTTGATGTTAAAGATCGTAAAGTCAAACCATCCCAAATTGTTTTCCATCGTAGAT
ACAATTTATCATACTATGATGTTTTCAGCCAAATCAAATTACAATTTCGAAAAACCATTN
GTNTGGTTAACATCAAAACNCCNCGGNAACAAGGCTGTCACTCTCGTTCAACAACCAACC
TTAAAATTACCAGAAACCGTTCTCGATTCCNACTTAATGAGTTTANATGAAAAANAAGTC
GCTGATGCTGCTGCCCNCCCNTTACCAGAAGACAATGATGACNTGTAAACNAATTTAAAA
AAAAAAAACAATAAAAAAAAATAATAATAATAATAATAAAGCCAACAATAAATTTCAATA
Length of full length seq. 720