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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSG128
Library VS
(Link to library)
Clone ID VSG128
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16175-1
Original site URL
Representative seq. ID VSG128E
(Link to Original site)
Representative DNA sequence 
>VSG128 (VSG128Q) /CSM/VS/VSG1-B/VSG128Q.Seq.d/
CAAAATGCCAATTCACAACAAAGTCTTAAGTAACGACCATTTCCGTAAACACTGGCAAAT
GCGTGTTAGAACCTGGTTCAACCAACCAGCACGTAAGATCAGAAGAAGAAATAACAGAAT
CGAAAAAGCTGCTAAAGTATTCCCAAGACCAATTGCCACCTTAAAACCAGTTGTTCGTGG
TTCAACCATCAGATACAACATGAAAGTTAGAGCTGGTCGTGGTTTCACCTTAGAGGAATT
AAAGGCTGCTGGTTTAACTGCTCAATACGCCAGAACCATCGGTATTGCTGTTGATACTCG
TCGTGTAAACAAAACCCAACAATCTTTAACCTTAAATACTCAACGTTTAAAGAACTACCA
ATCTAAACTCGTTCTTTTCCCACGTAAAGTCAATGCACCAAAGAAGGGTGAAGCCACCAA
GGAAGAAGTTGCTAAAGCTGTCCAAACCCTCAAACCATTCACTGTTAAATCCGCCATCGC
CGTTACTTGTGAACAAACCCCACGTAAACCAACTGAAGCTGAAAAGAAATTCTCTGCCTA
TGCCACCCTCAAAGCTGCTGACTCCAAAGCCAAGACTGTTGGTATCAGAAGAAAGGCTGC
NAAATCAAAGCTGCCAAAGCTGCNAAAAATAACTCTT
sequence update 2001. 3.25
Translated Amino Acid sequence 
KMPIHNKVLSNDHFRKHWQMRVRTWFNQPARKIRRRNNRIEKAAKVFPRPIATLKPVVRG
STIRYNMKVRAGRGFTLEELKAAGLTAQYARTIGIAVDTRRVNKTQQSLTLNTQRLKNYQ
SKLVLFPRKVNAPKKGEATKEEVAKAVQTLKPFTVKSAIAVTCEQTPRKPTEAEKKFSAY
ATLKAADSKAKTVGIRRKAAKSKLPKLXKITL
Translated Amino Acid sequence (All Frames) 
Frame A:
qnansqqslk*rpfp*tlanac*nlvqptst*dqkkk*qnrksc*sipktnchlktscsw
fnhqiqhes*swswfhlrgikgcwfncsirqnhrycc*yssckqnptifnlkystfkelp
i*trsfpt*sqctkeg*shqgrsc*scpnpqtihc*irhrryl*tnpt*tn*s*keilcl
chpqsc*lqsqdcwyqkkgcxikaakaaknns


Frame B:
KMPIHNKVLSNDHFRKHWQMRVRTWFNQPARKIRRRNNRIEKAAKVFPRPIATLKPVVRG
STIRYNMKVRAGRGFTLEELKAAGLTAQYARTIGIAVDTRRVNKTQQSLTLNTQRLKNYQ
SKLVLFPRKVNAPKKGEATKEEVAKAVQTLKPFTVKSAIAVTCEQTPRKPTEAEKKFSAY
ATLKAADSKAKTVGIRRKAAKSKLPKLXKITL


Frame C:
kcqfttks*vttisvntgkcvlepgstnqhvrseeeiteskkllkysqdqlpp*nqlfvv
qpsdtt*klelvvvsp*rn*rllv*llntpepsvlllilvv*tkpnnl*p*ilnv*rttn
lnsffshvksmhqrrvkpprkkllklskpsnhsllnppspllvnkphvnqlklkrnslpm
ppsklltpkprllvseerlxnqscqscxk*l
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSG128 (VSG128Q) /CSM/VS/VSG1-B/VSG128Q.Seq.d/                       1251   0.0
VSF395 (VSF395Q) /CSM/VS/VSF3-D/VSF395Q.Seq.d/                       1221   0.0
VSJ876 (VSJ876Q) /CSM/VS/VSJ8-D/VSJ876Q.Seq.d/                       1217   0.0
FC-AE16 (FC-AE16Q) /CSM/FC/FC-AE/FC-AE16Q.Seq.d/                     1211   0.0
FC-099 (FC-099Q) /CSM/CG/CG-FC/FC-099Q.Seq.d/                        1211   0.0
VSH659 (VSH659Q) /CSM/VS/VSH6-C/VSH659Q.Seq.d/                       1201   0.0
VSJ414 (VSJ414Q) /CSM/VS/VSJ4-A/VSJ414Q.Seq.d/                       1199   0.0
VSI517 (VSI517Q) /CSM/VS/VSI5-A/VSI517Q.Seq.d/                       1195   0.0
VHG289 (VHG289Q) /CSM/VH/VHG2-D/VHG289Q.Seq.d/                       1164   0.0
FC-BO22 (FC-BO22Q) /CSM/FC/FC-BO/FC-BO22Q.Seq.d/                     1152   0.0
own update 2003. 1. 9
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AX829368|AX829368.1 Sequence 88 from Patent WO03072602.            74   9e-12  2
AX818338|AX818338.1 Sequence 88 from Patent EP1338608.             74   9e-12  2
AY144954|AY144954.1 Saccharomyces castellii clone Contig1629 RPL13 gene, partial cds.    58   6e-11  3
AL402736|AL402736.1 T7 end of clone AT0AA004C12 of library AT0AA from strain CBS 4311 of Saccharomyces servazzii.    50   1e-10  3
Z47071|Z47071.1 S.cerevisiae chromosome XIII cosmid 9375.          74   5e-10  3
AY145049|AY145049.1 Kluyveromyces lactis clone P2258 RPL13 gene, complete cds.    68   1e-09  2
AY144953|AY144953.1 Saccharomyces castellii clone Contig2037 RPL13 gene, partial cds.    66   2e-09  2
AY144853|AY144853.1 Saccharomyces bayanus clone Contig4022 RPL13 gene, complete cds.    66   3e-09  2
BZ299426|BZ299426.1 KD0146.R1 Kluyveromyces delphensis Random Genomic Library Kluyveromyces delphensis genomic clone KD0146, DNA sequence.    50   4e-09  2
AL402820|AL402820.1 T7 end of clone AT0AA004H07 of library AT0AA from strain CBS 4311 of Saccharomyces servazzii.    50   2e-08  3
dna update 2004. 3.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54E20) RecName: Full=60S ribosomal protein L13;                     352   6e-96
(Q90YV5) RecName: Full=60S ribosomal protein L13;  &AF401567_1(A...   192   8e-48
BT043698_1(BT043698|pid:none) Salmo salar clone HM5_2256 ribosom...   180   3e-44
(Q90Z10) RecName: Full=60S ribosomal protein L13;  &AF385081_1(A...   178   1e-43
AY961553_1(AY961553|pid:none) Lysiphlebus testaceipes ribosomal ...   176   5e-43
BT058587_1(BT058587|pid:none) Salmo salar clone Contig2246 60S r...   175   1e-42
EU302538_1(EU302538|pid:none) Lineus viridis ribosomal protein r...   174   1e-42
AY395689_1(AY395689|pid:none) Solanum brevidens 60S ribosomal pr...   173   4e-42
BC041531_1(BC041531|pid:none) Xenopus laevis mRNA similar to rib...   173   4e-42
(P41125) RecName: Full=60S ribosomal protein L13; AltName: Full=...   172   5e-42
protein update 2009. 3.22
PSORT 

 psg: 0.55  gvh: 0.42  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.53  mip: 0.13
 nuc: 0.17  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.50  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 76.0 %: cytoplasmic
	 24.0 %: mitochondrial

>> prediction for VSG128 is cyt
5' end seq. ID VSG128F
5' end seq. 
>VSG128F.Seq
CAAAATGCCAATTCACAACAAAGTCTTAAGTAACGACCATTTCCGTAAACACTGGCAAAT
GCGTGTTAGAACCTGGTTCAACCAACCAGCACGTAAGATCAGAAGAAGAAATAACAGAAT
CGAAAAAGCTGCTAAAGTATTCCCAAGACCAATTGCCACCTTAAAACCAGTTGTTCGTGG
TTCAACCATCAGATACAACATGAAAGTTAGAGCTGGTCGTGGTTTCACCTTAGAGGAATT
AAAGGCTGCTGGTTTAACTGCTCAATACGCCAGAACCATCGGTATTGCTGTTGATACTCG
TCGTGTAAACAAAACCCAACAATCTTTAACCTTAAATACTCAACGTTTAAAGAACTACCA
ATCTAAACTCGTTCTTTTCCCACGTAAAGTCAATGCACCAAAGAAGGGTGAAGCC-----
-----
Length of 5' end seq. 415
3' end seq. ID VSG128Z
3' end seq. 
>VSG128Z.Seq
----------CAAAATGCCAATTCACAACAAAGTCTTAAGTAACGACCATTTCCGTAAAC
ACTGGCAAATGCGTGTTAGAACCTGGTTCAACCAACCAGCACGTAAGATCAGAAGAAGAA
ATAACAGAATCGAAAAAGCTGCTAAAGTATTCCCAAGACCAATTGCCACCTTAAAACCAG
TTGTTCGTGGTTCAACCATCAGATACAACATGAAAGTTAGAGCTGGTCGTGGTTTCACCT
TAGAGGAATTAAAGGCTGCTGGTTTAACTGCTCAATACGCCAGAACCATCGGTATTGCTG
TTGATACTCGTCGTGTAAACAAAACCCAACAATCTTTAACCTTAAATACTCAACGTTTAA
AGAACTACCAATCTAAACTCGTTCTTTTCCCACGTAAAGTCAATGCACCAAAGAAGGGTG
AAGCCACCAAGGAAGAAGTTGCTAAAGCTGTCCAAACCCTCAAACCATTCACTGTTAAAT
CCGCCATCGCCGTTACTTGTGAACAAACCCCACGTAAACCAACTGAAGCTGAAAAGAAAT
TCTCTGCCTATGCCACCCTCAAAGCTGCTGACTCCAAAGCCAAGACTGTTGGTATCAGAA
GAAAGGCTGCNAAATCAAAGCTGCCAAAGCTGCNAAAAATAACTCTT
Length of 3' end seq. 637
Connected seq. ID VSG128P
Connected seq. 
>VSG128P.Seq
CAAAATGCCAATTCACAACAAAGTCTTAAGTAACGACCATTTCCGTAAACACTGGCAAAT
GCGTGTTAGAACCTGGTTCAACCAACCAGCACGTAAGATCAGAAGAAGAAATAACAGAAT
CGAAAAAGCTGCTAAAGTATTCCCAAGACCAATTGCCACCTTAAAACCAGTTGTTCGTGG
TTCAACCATCAGATACAACATGAAAGTTAGAGCTGGTCGTGGTTTCACCTTAGAGGAATT
AAAGGCTGCTGGTTTAACTGCTCAATACGCCAGAACCATCGGTATTGCTGTTGATACTCG
TCGTGTAAACAAAACCCAACAATCTTTAACCTTAAATACTCAACGTTTAAAGAACTACCA
ATCTAAACTCGTTCTTTTCCCACGTAAAGTCAATGCACCAAAGAAGGGTGAAGCC-----
-----CAAAATGCCAATTCACAACAAAGTCTTAAGTAACGACCATTTCCGTAAACACTGG
CAAATGCGTGTTAGAACCTGGTTCAACCAACCAGCACGTAAGATCAGAAGAAGAAATAAC
AGAATCGAAAAAGCTGCTAAAGTATTCCCAAGACCAATTGCCACCTTAAAACCAGTTGTT
CGTGGTTCAACCATCAGATACAACATGAAAGTTAGAGCTGGTCGTGGTTTCACCTTAGAG
GAATTAAAGGCTGCTGGTTTAACTGCTCAATACGCCAGAACCATCGGTATTGCTGTTGAT
ACTCGTCGTGTAAACAAAACCCAACAATCTTTAACCTTAAATACTCAACGTTTAAAGAAC
TACCAATCTAAACTCGTTCTTTTCCCACGTAAAGTCAATGCACCAAAGAAGGGTGAAGCC
ACCAAGGAAGAAGTTGCTAAAGCTGTCCAAACCCTCAAACCATTCACTGTTAAATCCGCC
ATCGCCGTTACTTGTGAACAAACCCCACGTAAACCAACTGAAGCTGAAAAGAAATTCTCT
GCCTATGCCACCCTCAAAGCTGCTGACTCCAAAGCCAAGACTGTTGGTATCAGAAGAAAG
GCTGCNAAATCAAAGCTGCCAAAGCTGCNAAAAATAACTCTT
Length of connected seq. 1052
Full length Seq ID VSG128E
Full length Seq. 
>VSG128E.Seq
CAAAATGCCAATTCACAACAAAGTCTTAAGTAACGACCATTTCCGTAAACACTGGCAAAT
GCGTGTTAGAACCTGGTTCAACCAACCAGCACGTAAGATCAGAAGAAGAAATAACAGAAT
CGAAAAAGCTGCTAAAGTATTCCCAAGACCAATTGCCACCTTAAAACCAGTTGTTCGTGG
TTCAACCATCAGATACAACATGAAAGTTAGAGCTGGTCGTGGTTTCACCTTAGAGGAATT
AAAGGCTGCTGGTTTAACTGCTCAATACGCCAGAACCATCGGTATTGCTGTTGATACTCG
TCGTGTAAACAAAACCCAACAATCTTTAACCTTAAATACTCAACGTTTAAAGAACTACCA
ATCTAAACTCGTTCTTTTCCCACGTAAAGTCAATGCACCAAAGAAGGGTGAAGCCACCAA
GGAAGAAGTTGCTAAAGCTGTCCAAACCCTCAAACCATTCACTGTTAAATCCGCCATCGC
CGTTACTTGTGAACAAACCCCACGTAAACCAACTGAAGCTGAAAAGAAATTCTCTGCCTA
TGCCACCCTCAAAGCTGCTGACTCCAAAGCCAAGACTGTTGGTATCAGAAGAAAGGCTGC
NAAATCAAAGCTGCCAAAGCTGCNAAAAATAACTCTT
Length of full length seq. 637