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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSF854
Library VS
(Link to library)
Clone ID VSF854
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16491-1
Original site URL
Representative seq. ID VSF854E
(Link to Original site)
Representative DNA sequence 
>VSF854 (VSF854Q) /CSM/VS/VSF8-C/VSF854Q.Seq.d/
GCTGATATGAAAGACGAATGAATCCCAAGTCTTCAGAAAGATCAAATTAAGAGCTGAAGA
AGTTGAAGGTAGAAACGTTTTAACCAACTTTTACGGTATGGATTTAACCTCAGACAAACT
CCGTTCATTAATCCACAAATGGTCCACCTTGATCGAATGTTTTGTTGATGTTAAGACCAC
TGATGGTTATTTCCTCCGTGTCTTTGCCATCTGTTTCACCAAAAAGGGTGAATCCCAAAA
GAAAAAGACCTCATACGCCAAGACCTCCCGTGTTAAGGCTATCCGTAAGAAGATGGTTGA
CATTTTAACCGAAACCGTCTCATCCAACGACCTCAAAACCGTTGTTGACTATTTAATTGG
TGTCTCATTAAACGGTGTTACCACTAACGCCTCTGGTTTTGCCCCAACCCTCGCTGAAAA
AATCAGAATCGAAGGTTCATCAATCTTCCCAATCCACAACGTCTTCATCAGAAAGGTTAA
AGTCCTCAAGACTCCAAAAGTTGATGCTGCTAAATTATCTGAACTCTATGCTTCATCTGC
CACCTCAACCATCGCCCCAACTGAAGAAGTAGGTACCGCTGTTGAAAGAACTGAAGAAAC
CACCACCACCGCTTAAAAAAAAATAAAATAATNCCTATTTTAAAAA
sequence update 2001. 3.25
Translated Amino Acid sequence 
li*KTNESQVFRKIKLRAEEVEGRNVLTNFYGMDLTSDKLRSLIHKWSTLIECFVDVKTT
DGYFLRVFAICFTKKGESQKKKTSYAKTSRVKAIRKKMVDILTETVSSNDLKTVVDYLIG
VSLNGVTTNASGFAPTLAEKIRIEGSSIFPIHNVFIRKVKVLKTPKVDAAKLSELYASSA
TSTIAPTEEVGTAVERTEETTTTA*kkik*xlf*k
Translated Amino Acid sequence (All Frames) 
Frame A:
admkde*ipslqkdqiks*rs*r*krfnqllrygfnlrqtpfinpqmvhldrmfc*c*dh
*wlfppclchlfhqkg*ipkekdlirqdlpc*gyp*edg*hfnrnrliqrpqnrc*lfnw
clikrcyh*rlwfcpnpr*knqnrrfinlpnpqrlhqkg*spqdsks*cc*ii*tlcfic
hlnhrpn*rsryrc*kn*rnhhhrlkknkixpilk


Frame B:
li*KTNESQVFRKIKLRAEEVEGRNVLTNFYGMDLTSDKLRSLIHKWSTLIECFVDVKTT
DGYFLRVFAICFTKKGESQKKKTSYAKTSRVKAIRKKMVDILTETVSSNDLKTVVDYLIG
VSLNGVTTNASGFAPTLAEKIRIEGSSIFPIHNVFIRKVKVLKTPKVDAAKLSELYASSA
TSTIAPTEEVGTAVERTEETTTTA*kkik*xlf*k


Frame C:
*yerrmnpkssersn*elkklkvetf*ptftvwi*pqtnsvh*stngpp*snvllmlrpl
mvissvslpsvspkrvnpkrkrphtprppvlrlsvrrwltf*pkpshpttskpllti*lv
sh*tvlpltplvlpqpslkkseskvhqssqsttssserlkssrlqklmllnylnsmlhlp
pqpspqlkk*vpllkelkkpppplkkk*nnxyfk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSF854 (VSF854Q) /CSM/VS/VSF8-C/VSF854Q.Seq.d/                       1221   0.0
VSA592 (VSA592Q) /CSM/VS/VSA5-D/VSA592Q.Seq.d/                       1201   0.0
FC-BS17 (FC-BS17Q) /CSM/FC/FC-BS/FC-BS17Q.Seq.d/                     1201   0.0
FC-BO17 (FC-BO17Q) /CSM/FC/FC-BO/FC-BO17Q.Seq.d/                     1201   0.0
FC-BL12 (FC-BL12Q) /CSM/FC/FC-BL/FC-BL12Q.Seq.d/                     1201   0.0
FC-AK15 (FC-AK15Q) /CSM/FC/FC-AK/FC-AK15Q.Seq.d/                     1201   0.0
FC-AG13 (FC-AG13Q) /CSM/FC/FC-AG/FC-AG13Q.Seq.d/                     1201   0.0
FC-111 (FC-111Q) /CSM/CG/CG-FC/FC-111Q.Seq.d/                        1201   0.0
VSF675 (VSF675Q) /CSM/VS/VSF6-D/VSF675Q.Seq.d/                       1199   0.0
VSK462 (VSK462Q) /CSM/VS/VSK4-C/VSK462Q.Seq.d/                       1195   0.0
own update 2001.11.13
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AL397917|AL397917.1 T3 end of clone AS0AA005H09 of library AS0AA from strain CLIB 533 ofSaccharomyces bayanus.    60   1e-05  2
AL404835|AL404835.1 T3 end of clone XAT0AA002H02 of library XAT0AA from strain CBS 4311  of Saccharomyces servazzii.    48   2e-04  2
CF643875|CF643875.1 K13_F09 Filamentous Forced Diploid Ustilago maydis cDNA 3', mRNA sequence.    40   0.001  2
CF639788|CF639788.1 D19_G01 Filamentous Forced Diploid Ustilago maydis cDNA 3', mRNA sequence.    40   0.001  2
CD490233|CD490233.1 T34_G02 Teliospore Ustilago maydis cDNA 5', mRNA sequence.    40   0.001  2
CD490021|CD490021.1 T31_E07 Teliospore Ustilago maydis cDNA 5', mRNA sequence.    40   0.001  2
CF642755|CF642755.1 D55_F01 Filamentous Forced Diploid Ustilago maydis cDNA 3', mRNA sequence.    40   0.001  2
CD489854|CD489854.1 T29_A07 Teliospore Ustilago maydis cDNA 5', mRNA sequence.    40   0.001  2
CF639410|CF639410.1 D15_D02 Filamentous Forced Diploid Ustilago maydis cDNA 3', mRNA sequence.    40   0.001  2
CF644777|CF644777.1 K25_C11 Filamentous Forced Diploid Ustilago maydis cDNA 3', mRNA sequence.    40   0.001  2
dna update 2004. 3.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54ZW5) RecName: Full=40S ribosomal protein S3a;                     351   1e-95
AC116977_69(AC116977|pid:none) Dictyostelium discoideum chromoso...   350   2e-95
EU683898_1(EU683898|pid:none) Chironomus riparius ribosomal prot...   182   7e-45
(P49395) RecName: Full=40S ribosomal protein S3a; AltName: Full=...   179   8e-44
AY603568_1(AY603568|pid:none) Culicoides sonorensis clone CsRPS3...   176   5e-43
DQ241862_1(DQ241862|pid:none) Solanum tuberosum clone 027B03 40S...   176   5e-43
DQ252485_1(DQ252485|pid:none) Solanum tuberosum clone 048H05 cyc...   176   5e-43
DQ268849_1(DQ268849|pid:none) Solanum tuberosum clone 155F06 cyc...   176   5e-43
AB029635_1(AB029635|pid:none) Daucus carota mRNA for cyc07, comp...   176   6e-43
AY857460_1(AY857460|pid:none) Suberites domuncula S3a mRNA, comp...   175   8e-43
protein update 2009. 3.22
PSORT 

 psg: 0.47  gvh: 0.40  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.13  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: nuclear
	 28.0 %: cytoplasmic
	  8.0 %: vesicles of secretory system
	  4.0 %: mitochondrial

>> prediction for VSF854 is nuc
5' end seq. ID VSF854F
5' end seq. 
>VSF854F.Seq
GCTGATATGAAAGACGATGAATCCCAAGTCTTCAGAAAGATCAAATTAAGAGCTGAAGAA
GTTGAAGGTAGAAACGTTTTAACCAACTTTTACGGTATGGATTTAACCTCAGACAAACTC
CGTTCATTAATCCACAAATGGTCCACCTTGATCGAATGTTTTGTTGATGTTAAGACCACT
GATGGTTATTTCCTCCGTGTCTTTGCCATCTGTTTCACCAAAAAGGGTGAATCCCAAAAG
AAAAAGACCTCATACGCCAAGACCTCCCGTGTTAAGGCTATCCGTAAGAAGATGGTTGAC
ATTTTAACCGAAACCGTCTCATCCAACGACCTCAAAACCGTTGTTGACTATTTAATTGGT
GTCTCATTAAACGGTGTTACCACTAACGCCTCTGGTTTTGCCCCAACCCTCGCTGAAAAA
ATCAGAATCGAAGGTTCATCAATCTTCCCAATCCACAACGTCTTCATCAGAAAGGTTAAA
GTCCTCAAGA----------
Length of 5' end seq. 490
3' end seq. ID VSF854Z
3' end seq. 
>VSF854Z.Seq
----------GCTGATATGAAAGACGAATGAATCCCAAGTCTTCAGAAAGATCAAATTAA
GAGCTGAAGAAGTTGAAGGTAGAAACGTTTTAACCAACTTTTACGGTATGGATTTAACCT
CAGACAAACTCCGTTCATTAATCCACAAATGGTCCACCTTGATCGAATGTTTTGTTGATG
TTAAGACCACTGATGGTTATTTCCTCCGTGTCTTTGCCATCTGTTTCACCAAAAAGGGTG
AATCCCAAAAGAAAAAGACCTCATACGCCAAGACCTCCCGTGTTAAGGCTATCCGTAAGA
AGATGGTTGACATTTTAACCGAAACCGTCTCATCCAACGACCTCAAAACCGTTGTTGACT
ATTTAATTGGTGTCTCATTAAACGGTGTTACCACTAACGCCTCTGGTTTTGCCCCAACCC
TCGCTGAAAAAATCAGAATCGAAGGTTCATCAATCTTCCCAATCCACAACGTCTTCATCA
GAAAGGTTAAAGTCCTCAAGACTCCAAAAGTTGATGCTGCTAAATTATCTGAACTCTATG
CTTCATCTGCCACCTCAACCATCGCCCCAACTGAAGAAGTAGGTACCGCTGTTGAAAGAA
CTGAAGAAACCACCACCACCGCTTAAAAAAAAATAAAATAATNCCTATTTTAAAAA
Length of 3' end seq. 646
Connected seq. ID VSF854P
Connected seq. 
>VSF854P.Seq
GCTGATATGAAAGACGATGAATCCCAAGTCTTCAGAAAGATCAAATTAAGAGCTGAAGAA
GTTGAAGGTAGAAACGTTTTAACCAACTTTTACGGTATGGATTTAACCTCAGACAAACTC
CGTTCATTAATCCACAAATGGTCCACCTTGATCGAATGTTTTGTTGATGTTAAGACCACT
GATGGTTATTTCCTCCGTGTCTTTGCCATCTGTTTCACCAAAAAGGGTGAATCCCAAAAG
AAAAAGACCTCATACGCCAAGACCTCCCGTGTTAAGGCTATCCGTAAGAAGATGGTTGAC
ATTTTAACCGAAACCGTCTCATCCAACGACCTCAAAACCGTTGTTGACTATTTAATTGGT
GTCTCATTAAACGGTGTTACCACTAACGCCTCTGGTTTTGCCCCAACCCTCGCTGAAAAA
ATCAGAATCGAAGGTTCATCAATCTTCCCAATCCACAACGTCTTCATCAGAAAGGTTAAA
GTCCTCAAGA----------GCTGATATGAAAGACGAATGAATCCCAAGTCTTCAGAAAG
ATCAAATTAAGAGCTGAAGAAGTTGAAGGTAGAAACGTTTTAACCAACTTTTACGGTATG
GATTTAACCTCAGACAAACTCCGTTCATTAATCCACAAATGGTCCACCTTGATCGAATGT
TTTGTTGATGTTAAGACCACTGATGGTTATTTCCTCCGTGTCTTTGCCATCTGTTTCACC
AAAAAGGGTGAATCCCAAAAGAAAAAGACCTCATACGCCAAGACCTCCCGTGTTAAGGCT
ATCCGTAAGAAGATGGTTGACATTTTAACCGAAACCGTCTCATCCAACGACCTCAAAACC
GTTGTTGACTATTTAATTGGTGTCTCATTAAACGGTGTTACCACTAACGCCTCTGGTTTT
GCCCCAACCCTCGCTGAAAAAATCAGAATCGAAGGTTCATCAATCTTCCCAATCCACAAC
GTCTTCATCAGAAAGGTTAAAGTCCTCAAGACTCCAAAAGTTGATGCTGCTAAATTATCT
GAACTCTATGCTTCATCTGCCACCTCAACCATCGCCCCAACTGAAGAAGTAGGTACCGCT
GTTGAAAGAACTGAAGAAACCACCACCACCGCTTAAAAAAAAATAAAATAATNCCTATTT
TAAAAA
Length of connected seq. 1136
Full length Seq ID VSF854E
Full length Seq. 
>VSF854E.Seq
GCTGATATGAAAGACGAATGAATCCCAAGTCTTCAGAAAGATCAAATTAAGAGCTGAAGA
AGTTGAAGGTAGAAACGTTTTAACCAACTTTTACGGTATGGATTTAACCTCAGACAAACT
CCGTTCATTAATCCACAAATGGTCCACCTTGATCGAATGTTTTGTTGATGTTAAGACCAC
TGATGGTTATTTCCTCCGTGTCTTTGCCATCTGTTTCACCAAAAAGGGTGAATCCCAAAA
GAAAAAGACCTCATACGCCAAGACCTCCCGTGTTAAGGCTATCCGTAAGAAGATGGTTGA
CATTTTAACCGAAACCGTCTCATCCAACGACCTCAAAACCGTTGTTGACTATTTAATTGG
TGTCTCATTAAACGGTGTTACCACTAACGCCTCTGGTTTTGCCCCAACCCTCGCTGAAAA
AATCAGAATCGAAGGTTCATCAATCTTCCCAATCCACAACGTCTTCATCAGAAAGGTTAA
AGTCCTCAAGACTCCAAAAGTTGATGCTGCTAAATTATCTGAACTCTATGCTTCATCTGC
CACCTCAACCATCGCCCCAACTGAAGAAGTAGGTACCGCTGTTGAAAGAACTGAAGAAAC
CACCACCACCGCTTAAAAAAAAATAAAATAATNCCTATTTTAAAAA
Length of full length seq. 646