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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSF827
Library VS
(Link to library)
Clone ID VSF827
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VSF827P
(Link to Original site)
Representative DNA sequence 
>VSF827 (VSF827Q) /CSM/VS/VSF8-B/VSF827Q.Seq.d/
ATTTAGCTCACTACATAAAATGGAAAAGATTTACTCAAAAGGTGTCGTTTTAGGTTACAG
AAGATCACAAGCTACTCAATACCCAAACATCAGTTTAATCAAAATTGAAGGTGTTGTCTC
ACGTGAAGATTCAACTTTTTATTTAGGTAAAAAAGTCTGTTTAGTCAGCAAAGTTTCCAA
ATCAGCCAAAAACCCAACTGGTCACAAAATTTCATGGGGTAAGATTTGCAAAACCCACGG
TAGTTCAGGTGTTGTCCAAGCTCGTTTCGCCAGAAACCTCCCACCAAAAGCTATGGGTGC
CCCAGTCAGAGTTATGTTATACCCATCAAACATCTAAATAAATCTTTAAAACGAAAAAAA
AAAXXXXXXXXXXATTTAGCTCACTACATAAAATGGAAAAGATTTACTCAAAAGGTGTCG
TTTTAGGTTACAGAAGATCACAAGCTACTCAATACCCAAACATCAGTTTAATCAAAATTG
AAGGTGTTGTCTCACGTGAAGATTCAACTTTTTATTTAGGTAAAAAAGTCTGTTTAGTCA
GCAAAGTTTCCAAATCAGCCAAAAACCCAACTGGTCACAAAATTTCATGGGGTAAGATTT
GCAAAACCCACGGTAGTTCAGGTGTTGTCCAAGCTCGTTTCGCCAGAAACCTCCCACCAA
AAGCTATGGGTGCCCCAGTCAGAGTTATGTTATACCCATCAAACANCTAAATAA
sequence update 2001. 3.22
Translated Amino Acid sequence 
i*ltt*ngkdllkrcrfrlqkitsysipkhqfnqn*rcclt*rfnflfr*kslfsqqsfq
isqkpnwsqnfmg*dlqnpr*FRCCPSSFRQKPPTKSYGCPSQSYVIPIKHLNKSLKRKK
K---

---FSSLHKMEKIYSKGVVLGYRRSQATQYPNISLIKIEGVVSREDSTFYLGKKVCLVSK
VSKSAKNPTGHKISWGKICKTHGSSGVVQARFARNLPPKAMGAPVRVMLYPSNX*i
Translated Amino Acid sequence (All Frames) 
Frame A:
i*ltt*ngkdllkrcrfrlqkitsysipkhqfnqn*rcclt*rfnflfr*kslfsqqsfq
isqkpnwsqnfmg*dlqnpr*FRCCPSSFRQKPPTKSYGCPSQSYVIPIKHLNKSLKRKK
K---

---i*ltt*ngkdllkrcrfrlqkitsysipkhqfnqn*rcclt*rfnflfr*kslfsqq
sfqisqkpnwsqnfmg*dlqnpr*frccpssfrqkpptksygcpsqsyvipikxln

Frame B:
fsslhkmekiyskgvvlgyrrsqatqypnislikiegvvsredstfylgkkvclvskvsk
saknptghkiswgkickthgssgvvqarfarnlppkamgapvrvmlypsni*inl*nekk
---

---FSSLHKMEKIYSKGVVLGYRRSQATQYPNISLIKIEGVVSREDSTFYLGKKVCLVSK
VSKSAKNPTGHKISWGKICKTHGSSGVVQARFARNLPPKAMGAPVRVMLYPSNX*i

Frame C:
lahyikwkrftqkvsf*vtedhkllntqtsv*sklkvlshvkiqlfi*vkksv*sakfpn
qpktqlvtkfhgvrfakptvvqvlsklvspetshqklwvpqselcythqtsk*ifktkkk
---

---lahyikwkrftqkvsf*vtedhkllntqtsv*sklkvlshvkiqlfi*vkksv*sak
fpnqpktqlvtkfhgvrfakptvvqvlsklvspetshqklwvpqselcythqtxk*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSK108 (VSK108Q) /CSM/VS/VSK1-A/VSK108Q.Seq.d/                        700   0.0
VSJ573 (VSJ573Q) /CSM/VS/VSJ5-D/VSJ573Q.Seq.d/                        700   0.0
VSJ462 (VSJ462Q) /CSM/VS/VSJ4-C/VSJ462Q.Seq.d/                        700   0.0
VSJ120 (VSJ120Q) /CSM/VS/VSJ1-A/VSJ120Q.Seq.d/                        700   0.0
VSH370 (VSH370Q) /CSM/VS/VSH3-C/VSH370Q.Seq.d/                        700   0.0
VSF827 (VSF827Q) /CSM/VS/VSF8-B/VSF827Q.Seq.d/                        700   0.0
VSE875 (VSE875Q) /CSM/VS/VSE8-D/VSE875Q.Seq.d/                        700   0.0
VSD188 (VSD188Q) /CSM/VS/VSD1-D/VSD188Q.Seq.d/                        700   0.0
VSC278 (VSC278Q) /CSM/VS/VSC2-D/VSC278Q.Seq.d/                        700   0.0
VSA628 (VSA628Q) /CSM/VS/VSA6-B/VSA628Q.Seq.d/                        700   0.0
own update 2001.11.13
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BQ834795|BQ834795.1 Po_ad_03E06_TEXF1 Psoroptes ovis mixed Psoroptes ovis cDNA clone Po_ad_03E06 5' similar to BAB27124 ES CELLS CDNA, RIKEN FULL-LENGTH ENRICHE FULL INSERT, mRNA sequence.    36  0.13  2
BG211495|BG211495.1 RST31062 Athersys RAGE Library Homo sapiens cDNA, mRNA sequence.    38  0.69  2
AC116574|AC116574.2 Mus musculus chromosome UNK clone RP23-234F20, WORKING DRAFT SEQUENCE, 92 unordered pieces.    40  0.99  3
D17450|D17450.1 Mycoplasma salivarium gene for aminopeptidase, complete cds.    34  1.6  2
AC142285|AC142285.1 Pan troglodytes chromosome 7 clone RP43-169B4, complete sequence.    42  1.7  2
AC125010|AC125010.3 Mus musculus clone RP24-117L12, WORKING DRAFT SEQUENCE, 7 unordered pieces.    40  2.0  2
AL606531|AL606531.6 Mouse DNA sequence *** SEQUENCING CANCELLED *** from clone  RP23-104H11.    34  2.6  4
AC133582|AC133582.3 Mus musculus clone RP24-440G8, WORKING DRAFT SEQUENCE, 11 unordered pieces.    34  3.3  3
AL645602|AL645602.14 Mouse DNA sequence from clone RP23-79E13 on chromosome 11.    34  3.8  4
AX344895|AX344895.1 Sequence 320 from Patent WO0200927.           40  4.3  2
dna update 2003. 8.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FB899478_1(FB899478|pid:none) Sequence 118751 from Patent WO2008...   103   5e-21
FB899466_1(FB899466|pid:none) Sequence 118739 from Patent WO2008...   103   6e-21
FJ362592_1(FJ362592|pid:none) Vernicia fordii 60S ribosomal prot...   103   6e-21
EF144854_1(EF144854|pid:none) Populus trichocarpa clone WS01119_...   103   6e-21
(Q9FZH0) RecName: Full=60S ribosomal protein L35a-2;  &AC008046_...   102   8e-21
FB899468_1(FB899468|pid:none) Sequence 118741 from Patent WO2008...   102   8e-21
(P51422) RecName: Full=60S ribosomal protein L35a-4;  &AF446895_...   102   1e-20
AY857450_1(AY857450|pid:none) Suberites domuncula L35a mRNA, com...   102   1e-20
(Q9C912) RecName: Full=60S ribosomal protein L35a-3;  &AC020579_...   102   1e-20
BT051287_1(BT051287|pid:none) Medicago truncatula clone MTYF1_F2...   101   2e-20
protein update 2009. 3.22
PSORT 

 psg: 0.70  gvh: 0.21  alm: 0.53  top: 0.53  tms: 0.00  mit: 0.51  mip: 0.11
 nuc: 0.08  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: mitochondrial
	 28.0 %: nuclear
	 12.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for VSF827 is mit
5' end seq. ID VSF827F
5' end seq. 
>VSF827F.Seq
ATTTAGCTCACTACATAAAATGGAAAAGATTTACTCAAAAGGTGTCGTTTTAGGTTACAG
AAGATCACAAGCTACTCAATACCCAAACATCAGTTTAATCAAAATTGAAGGTGTTGTCTC
ACGTGAAGATTCAACTTTTTATTTAGGTAAAAAAGTCTGTTTAGTCAGCAAAGTTTCCAA
ATCAGCCAAAAACCCAACTGGTCACAAAATTTCATGGGGTAAGATTTGCAAAACCCACGG
TAGTTCAGGTGTTGTCCAAGCTCGTTTCGCCAGAAACCTCCCACCAAAAGCTATGGGTGC
CCCAGTCAGAGTTATGTTATACCCATCAAACATCTAAATAAATCTTTAAAACGAAAAAAA
AAA----------
Length of 5' end seq. 363
3' end seq. ID VSF827Z
3' end seq. 
>VSF827Z.Seq
----------ATTTAGCTCACTACATAAAATGGAAAAGATTTACTCAAAAGGTGTCGTTT
TAGGTTACAGAAGATCACAAGCTACTCAATACCCAAACATCAGTTTAATCAAAATTGAAG
GTGTTGTCTCACGTGAAGATTCAACTTTTTATTTAGGTAAAAAAGTCTGTTTAGTCAGCA
AAGTTTCCAAATCAGCCAAAAACCCAACTGGTCACAAAATTTCATGGGGTAAGATTTGCA
AAACCCACGGTAGTTCAGGTGTTGTCCAAGCTCGTTTCGCCAGAAACCTCCCACCAAAAG
CTATGGGTGCCCCAGTCAGAGTTATGTTATACCCATCAAACANCTAAATAA
Length of 3' end seq. 341
Connected seq. ID VSF827P
Connected seq. 
>VSF827P.Seq
ATTTAGCTCACTACATAAAATGGAAAAGATTTACTCAAAAGGTGTCGTTTTAGGTTACAG
AAGATCACAAGCTACTCAATACCCAAACATCAGTTTAATCAAAATTGAAGGTGTTGTCTC
ACGTGAAGATTCAACTTTTTATTTAGGTAAAAAAGTCTGTTTAGTCAGCAAAGTTTCCAA
ATCAGCCAAAAACCCAACTGGTCACAAAATTTCATGGGGTAAGATTTGCAAAACCCACGG
TAGTTCAGGTGTTGTCCAAGCTCGTTTCGCCAGAAACCTCCCACCAAAAGCTATGGGTGC
CCCAGTCAGAGTTATGTTATACCCATCAAACATCTAAATAAATCTTTAAAACGAAAAAAA
AAA----------ATTTAGCTCACTACATAAAATGGAAAAGATTTACTCAAAAGGTGTCG
TTTTAGGTTACAGAAGATCACAAGCTACTCAATACCCAAACATCAGTTTAATCAAAATTG
AAGGTGTTGTCTCACGTGAAGATTCAACTTTTTATTTAGGTAAAAAAGTCTGTTTAGTCA
GCAAAGTTTCCAAATCAGCCAAAAACCCAACTGGTCACAAAATTTCATGGGGTAAGATTT
GCAAAACCCACGGTAGTTCAGGTGTTGTCCAAGCTCGTTTCGCCAGAAACCTCCCACCAA
AAGCTATGGGTGCCCCAGTCAGAGTTATGTTATACCCATCAAACANCTAAATAA
Length of connected seq. 704
Full length Seq ID -
Full length Seq. -
Length of full length seq. -