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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSF814
Library VS
(Link to library)
Clone ID VSF814
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U01389-1
Original site URL
Representative seq. ID VSF814P
(Link to Original site)
Representative DNA sequence 
>VSF814 (VSF814Q) /CSM/VS/VSF8-A/VSF814Q.Seq.d/
CTTTTCACAAATAAAACTAATATCAATTTATATAATAATTATTCAAAATCATCAAAATCA
GGTAAGAAATCAAAGAAAGATGAAGATGATGAAGATGGAGAAATTGAAACAAGTAAAACA
AGTAAAAAATCAGCATCTTCATCATCAATGGAGAATGTTTTAAAAGAATTAGAGAAAAGT
TTTGGTAAAGGTACATTAATGAAATTAGGGTCCCAATTTTCAACTCAAAAAGTTGAAGTT
ATACCATCAGGATCAATGGGATTAGATATTGCATTAGGAGTAGGAGGTTTACCAAAAGGT
AGAGTCACTGAAATATTTGGACCTGAAAGTAGTGGTAAAACAACTTTAGCATTACATGTC
ATTGCTCAAGCTCAAAAAGCTGGAGGAAATTGTACTTTTATCGATGCTGAACATGCACTA
AACCCACAATGGGCTGCACGTTTAGGTGTAAATTTAGATGAACTATTCGTTTCTCAACCT
GACAATGGTGAACAAGCATTAGAAATCGTTGATTCTTTACTTCGTTCAAAAACAATGTCT
GTTAXXXXXXXXXXTTTAAAGAGGCAATTTTTGATATTGATTTCCCAATCTGGGTATAAA
TAAAACTGGTGAAATCATTGATCTTGCAGTTGCAGAAGGAATTATAGATAAAATGGGATC
TTGGTATAGTTATAACGATATTAAATTAGACCAAGGTAGGGAAAAGACTAAATACCTTTT
AGAAAAGACTCAACCAAATCTTTTAGTAGAAATTGAAAATAAATTAAGAGATAAATTAAT
TAAATCAAAACCATTAATTAATCAACAACAAGAAGAAGAAGGAAATGATCAAACCAGTGA
TGAATTTGATATTGAAAATGATGATGAAATTATAGAAGAAGATATTGATGATGAAACTAT
TAAAAAATAAATAATTGAATTAATTAATTTTAACCAACATCAATTTGATTTCCAAATATA
TAAATTCTGAAAAATAGAATAATTATTTTCTTATATCAAATTTAAATTATAATAATAATA
ATAATAATAATAAATTAAAATAAAATAAATGTTATTA
sequence update 2001. 3.22
Translated Amino Acid sequence 
LFTNKTNINLYNNYSKSSKSGKKSKKDEDDEDGEIETSKTSKKSASSSSMENVLKELEKS
FGKGTLMKLGSQFSTQKVEVIPSGSMGLDIALGVGGLPKGRVTEIFGPESSGKTTLALHV
IAQAQKAGGNCTFIDAEHALNPQWAARLGVNLDELFVSQPDNGEQALEIVDSLLRSKTMS
V---

---LKRQFLILISQSGYK*nw*nh*scscrrnyr*ngilv*l*ry*irpr*gkd*ipfrk
dstksfsrn*k*ikr*in*iktin*sttrrrrk*snq**i*y*k***nyrrry***ny*k
inn*in*f*ptsi*fpni*ilknriiiflyqi*iiiiiiiiin*nkinvi
Translated Amino Acid sequence (All Frames) 
Frame A:
LFTNKTNINLYNNYSKSSKSGKKSKKDEDDEDGEIETSKTSKKSASSSSMENVLKELEKS
FGKGTLMKLGSQFSTQKVEVIPSGSMGLDIALGVGGLPKGRVTEIFGPESSGKTTLALHV
IAQAQKAGGNCTFIDAEHALNPQWAARLGVNLDELFVSQPDNGEQALEIVDSLLRSKTMS
V---

---fkeaifdidfpiwv*iklvkslilqlqkel*ikwdlgivitiln*tkvgkrlntf*k
rlnqif**klkin*ein*lnqnh*linnkkkkemikpvmnlilkmmmkl*kkilmmkllk
nk*ln*liltninliskyinsek*nnyflisnlnynnnnnnnklk*nkcy

Frame B:
fsqiklisiyiiiiqnhqnqvrnqrkmkmmkmeklkqvkqvknqhlhhqwrmf*kn*rkv
lvkvh**n*gpnfqlkklklyhqdqwd*ilh*e*evyqkveslkyldlkvvvkql*hyms
llklkkleeivllsmlnmh*thnglhv*v*i*mnysflnltmvnkh*kslilyfvqkqcl
l---

---LKRQFLILISQSGYK*nw*nh*scscrrnyr*ngilv*l*ry*irpr*gkd*ipfrk
dstksfsrn*k*ikr*in*iktin*sttrrrrk*snq**i*y*k***nyrrry***ny*k
inn*in*f*ptsi*fpni*ilknriiiflyqi*iiiiiiiiin*nkinvi

Frame C:
fhk*n*yqfi**lfkiikir*eiker*r**rwrn*nk*nk*kisifiingecfkrirekf
w*ryineirvpifnsks*sytiringirycirsrrftkr*sh*niwt*k*w*nnfsitch
cssskswrklyfyrc*tctkptmgctfrckfr*tirfst*qw*tsirnr*fftsfknnvc
---

---*rgnf*y*fpnlginktgeiidlavaegiidkmgswysyndikldqgrektkyllek
tqpnllveienklrdklikskplinqqqeeegndqtsdefdienddeiieediddetikk
*iielinfnqhqfdfqiykf*kie*lfsyikfkl********ikik*mll
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSF814 (VSF814Q) /CSM/VS/VSF8-A/VSF814Q.Seq.d/                       1600   0.0
VSH660 (VSH660Q) /CSM/VS/VSH6-C/VSH660Q.Seq.d/                       1031   0.0
SLI311 (SLI311Q) /CSM/SL/SLI3-A/SLI311Q.Seq.d/                        529   e-149
SSL477 (SSL477Q) /CSM/SS/SSL4-D/SSL477Q.Seq.d/                        402   e-111
CHI755 (CHI755Q) /CSM/CH/CHI7-C/CHI755Q.Seq.d/                         46   0.001
VSI481 (VSI481Q) /CSM/VS/VSI4-D/VSI481Q.Seq.d/                         42   0.017
VSF352 (VSF352Q) /CSM/VS/VSF3-C/VSF352Q.Seq.d/                         42   0.017
VHC794 (VHC794Q) /CSM/VH/VHC7-D/VHC794Q.Seq.d/                         42   0.017
VHC364 (VHC364Q) /CSM/VH/VHC3-C/VHC364Q.Seq.d/                         42   0.017
VFO619 (VFO619Q) /CSM/VF/VFO6-A/VFO619Q.Seq.d/                         42   0.017
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116988|AC116988.2 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.   952  0.0  11
AY135423|AY135423.1 Listeria ivanovii strain FSLc2010 RecA (recA) gene, complete cds.    38  2e-09  4
AY135422|AY135422.1 Listeria ivanovii strain FSLc2011 RecA (recA) gene, complete cds.    38  2e-09  4
AF191135|AF191135.1 Acinetobacter genomosp. 6 RecA protein (recA) gene, partial cds.    56  3e-07  2
AE010566|AE010566.1 Fusobacterium nucleatum subsp. nucleatum ATCC 25586, section 108 of 197 of the complete genome.    38  2e-06  6
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    42  5e-06  10
BX128251|BX128251.1 Danio rerio genomic clone DKEY-279C3, genomic survey sequence.    38  9e-06  4
AY229701|AY229701.1 Listeria monocytogenes isolate 105 serotype 1/2a RecA (recA) gene, partial cds.    40  1e-05  3
AY229740|AY229740.1 Listeria monocytogenes isolate 149 serotype 1/2a RecA (recA) gene, partial cds.    40  1e-05  3
AY229708|AY229708.1 Listeria monocytogenes isolate 195 serotype 1/2a RecA (recA) gene, partial cds.    40  1e-05  3
dna update 2003. 8.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P62209) RecName: Full=Protein recA; AltName: Full=Recombinase A...   185   2e-45
CP000489_592(CP000489|pid:none) Paracoccus denitrificans PD1222 ...   182   2e-44
(A3PLJ2) Protein recA (Recombinase A).  &CP000143_2056(CP000143|...   182   2e-44
AJ294385_1(AJ294385|pid:none) Mycoplana dimorpha partial recA ge...   181   5e-44
CP000699_507(CP000699|pid:none) Sphingomonas wittichii RW1, comp...   180   6e-44
(Q3ST50) Protein recA (Recombinase A).  &CP000115_1273(CP000115|...   180   6e-44
CP001196_1411(CP001196|pid:none) Oligotropha carboxidovorans OM5...   180   8e-44
CP001029_2590(CP001029|pid:none) Methylobacterium populi BJ001, ...   180   8e-44
(P95469) Protein recA (Recombinase A).  &U59631_1(U59631|pid:none)    180   8e-44
(Q2W9P9) Protein recA (Recombinase A).  &AP007255_622(AP007255|p...   179   1e-43
protein update 2009. 3.22
PSORT 

 psg: 0.66  gvh: 0.18  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.33  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: nuclear
	 32.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for VSF814 is nuc
5' end seq. ID VSF814F
5' end seq. 
>VSF814F.Seq
CTTTTCACAAATAAAACTAATATCAATTTATATAATAATTATTCAAAATCATCAAAATCA
GGTAAGAAATCAAAGAAAGATGAAGATGATGAAGATGGAGAAATTGAAACAAGTAAAACA
AGTAAAAAATCAGCATCTTCATCATCAATGGAGAATGTTTTAAAAGAATTAGAGAAAAGT
TTTGGTAAAGGTACATTAATGAAATTAGGGTCCCAATTTTCAACTCAAAAAGTTGAAGTT
ATACCATCAGGATCAATGGGATTAGATATTGCATTAGGAGTAGGAGGTTTACCAAAAGGT
AGAGTCACTGAAATATTTGGACCTGAAAGTAGTGGTAAAACAACTTTAGCATTACATGTC
ATTGCTCAAGCTCAAAAAGCTGGAGGAAATTGTACTTTTATCGATGCTGAACATGCACTA
AACCCACAATGGGCTGCACGTTTAGGTGTAAATTTAGATGAACTATTCGTTTCTCAACCT
GACAATGGTGAACAAGCATTAGAAATCGTTGATTCTTTACTTCGTTCAAAAACAATGTCT
GTTA----------
Length of 5' end seq. 544
3' end seq. ID VSF814Z
3' end seq. 
>VSF814Z.Seq
----------TTTAAAGAGGCAATTTTTGATATTGATTTCCCAATCTGGGTATAAATAAA
ACTGGTGAAATCATTGATCTTGCAGTTGCAGAAGGAATTATAGATAAAATGGGATCTTGG
TATAGTTATAACGATATTAAATTAGACCAAGGTAGGGAAAAGACTAAATACCTTTTAGAA
AAGACTCAACCAAATCTTTTAGTAGAAATTGAAAATAAATTAAGAGATAAATTAATTAAA
TCAAAACCATTAATTAATCAACAACAAGAAGAAGAAGGAAATGATCAAACCAGTGATGAA
TTTGATATTGAAAATGATGATGAAATTATAGAAGAAGATATTGATGATGAAACTATTAAA
AAATAAATAATTGAATTAATTAATTTTAACCAACATCAATTTGATTTCCAAATATATAAA
TTCTGAAAAATAGAATAATTATTTTCTTATATCAAATTTAAATTATAATAATAATAATAA
TAATAATAAATTAAAATAAAATAAATGTTATTA
Length of 3' end seq. 503
Connected seq. ID VSF814P
Connected seq. 
>VSF814P.Seq
CTTTTCACAAATAAAACTAATATCAATTTATATAATAATTATTCAAAATCATCAAAATCA
GGTAAGAAATCAAAGAAAGATGAAGATGATGAAGATGGAGAAATTGAAACAAGTAAAACA
AGTAAAAAATCAGCATCTTCATCATCAATGGAGAATGTTTTAAAAGAATTAGAGAAAAGT
TTTGGTAAAGGTACATTAATGAAATTAGGGTCCCAATTTTCAACTCAAAAAGTTGAAGTT
ATACCATCAGGATCAATGGGATTAGATATTGCATTAGGAGTAGGAGGTTTACCAAAAGGT
AGAGTCACTGAAATATTTGGACCTGAAAGTAGTGGTAAAACAACTTTAGCATTACATGTC
ATTGCTCAAGCTCAAAAAGCTGGAGGAAATTGTACTTTTATCGATGCTGAACATGCACTA
AACCCACAATGGGCTGCACGTTTAGGTGTAAATTTAGATGAACTATTCGTTTCTCAACCT
GACAATGGTGAACAAGCATTAGAAATCGTTGATTCTTTACTTCGTTCAAAAACAATGTCT
GTTA----------TTTAAAGAGGCAATTTTTGATATTGATTTCCCAATCTGGGTATAAA
TAAAACTGGTGAAATCATTGATCTTGCAGTTGCAGAAGGAATTATAGATAAAATGGGATC
TTGGTATAGTTATAACGATATTAAATTAGACCAAGGTAGGGAAAAGACTAAATACCTTTT
AGAAAAGACTCAACCAAATCTTTTAGTAGAAATTGAAAATAAATTAAGAGATAAATTAAT
TAAATCAAAACCATTAATTAATCAACAACAAGAAGAAGAAGGAAATGATCAAACCAGTGA
TGAATTTGATATTGAAAATGATGATGAAATTATAGAAGAAGATATTGATGATGAAACTAT
TAAAAAATAAATAATTGAATTAATTAATTTTAACCAACATCAATTTGATTTCCAAATATA
TAAATTCTGAAAAATAGAATAATTATTTTCTTATATCAAATTTAAATTATAATAATAATA
ATAATAATAATAAATTAAAATAAAATAAATGTTATTA
Length of connected seq. 1047
Full length Seq ID -
Full length Seq. -
Length of full length seq. -