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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSF803
Library VS
(Link to library)
Clone ID VSF803
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VSF803P
(Link to Original site)
Representative DNA sequence 
>VSF803 (VSF803Q) /CSM/VS/VSF8-A/VSF803Q.Seq.d/
CCACGCCGTCCGACATATATTAATAGAATTGTAATAAAATGTCAAAAGTAATTACAGGAA
AGCAATACTCTTGGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGCAG
TCGATGGTAAAGTTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGAAG
TTTTACTTTTAGCAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAATGT
CAGATAAACCAACATCAATTTTTTAAAGGAATTATGAAATTGGATATATTTCATCATATG
AACATCCAAAATTTGTTCAAAAATCAGATTTCTACXXXXXXXXXXTTTGTTTTCTTCCGT
TTCATCCTTCCACTCCGTTATCATAGCTTTACAGATTTAATTTGTTATTTCCTCATTGCT
GAATTCGTCTTTGGTTGGTATCTCACAATTAATTTCCAAGTTAGTCATGTCGCTGAAGAT
CTCAAATTCTTTGCTACCCCTGAAAGACCAGATGAACCATCTCAAATCAATGAAGATTGG
GCAATCCTTCAACTTAAAACTACTCAAGATTATGGTCATGGTTCACTCCTTTGTACCTTT
TTTAGTGGTTCTTTAAATCATCAAGTTGTTCATCATTTATTCCCATCAATTGCTCAAGAT
TTCTACCCACAACTTGTACCAATTGTAAAAGAAGTTTGTAAAGAACATAACATTACTTAC
CACATTAAACCAAACTTCACTGAAGCTATTATGTCACACATTAATTACCTTTACAAAATG
GGTAATGANCCAGATTATGTTAAAAAACCATTAGCCTCAAAAGATGATTAAATGAAATAA
CTTAAAAACCAATTATTTACTTGACAAACAGTAAT
sequence update 2001. 3.22
Translated Amino Acid sequence 
prrptyinrivikcqk*lqesntlgqs*pnitlkmivglqsmvkfmilqdgyhyiqvvkk
fyf*QQVEMLQIYLKVIIQCQINQHQFFKGIMKLDIFHHMNIQNLFKNQIS---

---FVFFRFILPLRYHSFTDLICYFLIAEFVFGWYLTINFQVSHVAEDLKFFATPERPDE
PSQINEDWAILQLKTTQDYGHGSLLCTFFSGSLNHQVVHHLFPSIAQDFYPQLVPIVKEV
CKEHNITYHIKPNFTEAIMSHINYLYKMGNXPDYVKKPLASKDD*mk*lknqlft*qtv
Translated Amino Acid sequence (All Frames) 
Frame A:
prrptyinrivikcqk*lqesntlgqs*pnitlkmivglqsmvkfmilqdgyhyiqvvkk
fyf*QQVEMLQIYLKVIIQCQINQHQFFKGIMKLDIFHHMNIQNLFKNQIS---

---FVFFRFILPLRYHSFTDLICYFLIAEFVFGWYLTINFQVSHVAEDLKFFATPERPDE
PSQINEDWAILQLKTTQDYGHGSLLCTFFSGSLNHQVVHHLFPSIAQDFYPQLVPIVKEV
CKEHNITYHIKPNFTEAIMSHINYLYKMGNXPDYVKKPLASKDD*mk*lknqlft*qtv

Frame B:
havrhiliel**nvksnyrkaillvrvsqt*h*k*llgcsrw*sl*yykmgtitsrw*rs
ftfssr*rcykfi*klssnvr*tninflkel*nwiyfii*tskicskirfl---

---lfssvssfhsviialqi*fvissllnsslvgisqlisklvmslkisnsllplkdqmn
hlksmkigqsfnlkllkimvmvhsfvpflvvl*iiklfiiyshqllkisthnlyql*kkf
vknitllttlnqtslkllchtlitftkwvmxqimlknh*pqkmik*nnlktnylldkq*

Frame C:
tpsdiy**ncnkmskvitgkqyswselakhntendcwvavdgkvyditrwvplhpggkev
lllaagrdvtnlfesyhpmsdkptsif*rnyeigyissyehpkfvqksdfy---

---cflpfhpstpls*lyrfnllfphc*irlwlvshn*fps*scr*rsqilcyp*ktr*t
isnq*rlgnpst*nysrlwswftplylf*wffkssscssfipincsrflpttctnckrsl
*rt*hylph*tklh*syyvth*lplqng**xrlc*ktislkr*lneit*kpiiyltnsn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSF803 (VSF803Q) /CSM/VS/VSF8-A/VSF803Q.Seq.d/                       1669   0.0
VSC833 (VSC833Q) /CSM/VS/VSC8-B/VSC833Q.Seq.d/                       1027   0.0
SSB813 (SSB813Q) /CSM/SS/SSB8-A/SSB813Q.Seq.d/                       1027   0.0
SLE621 (SLE621Q) /CSM/SL/SLE6-A/SLE621Q.Seq.d/                       1027   0.0
SLA259 (SLA259Q) /CSM/SL/SLA2-C/SLA259Q.Seq.d/                       1027   0.0
SLA256 (SLA256Q) /CSM/SL/SLA2-C/SLA256Q.Seq.d/                       1027   0.0
SLG121 (SLG121Q) /CSM/SL/SLG1-A/SLG121Q.Seq.d/                       1013   0.0
SLC603 (SLC603Q) /CSM/SL/SLC6-A/SLC603Q.Seq.d/                        977   0.0
CFC256 (CFC256Q) /CSM/CF/CFC2-C/CFC256Q.Seq.d/                        969   0.0
VFN880 (VFN880Q) /CSM/VF/VFN8-D/VFN880Q.Seq.d/                        963   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AB029311|AB029311.1 Dictyostelium discoideum FadA gene for fatty acid desaturase, complete cds.   583  0.0  6
BD092935|BD092935.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids in plants.  1019  0.0  2
BD082645|BD082645.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids.  1019  0.0  2
BD082630|BD082630.1 Methods and compositions for synthesis of long chain poly-unsaturated fatty acids.  1019  0.0  2
AB022097|AB022097.1 Dictyostelium discoideum mRNA for delta 5 fatty acid desaturase, complete cds.   149  2e-97  7
BX537102|BX537102.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone DKEY-276L17.    36  0.17  7
BZ067998|BZ067998.1 lki76e10.b1 B.oleracea002 Brassica oleracea genomic, DNA sequence.    46  0.73  1
BZ033449|BZ033449.1 oej70g10.b1 B.oleracea002 Brassica oleracea genomic, DNA sequence.    46  0.73  1
AC102603|AC102603.2 Mus musculus clone RP23-385I17, WORKING DRAFT SEQUENCE, 13 unordered pieces.    38  0.94  3
AJ400907|AJ400907.1 Drosophila melanogaster partial mitochondrial genome.    38  1.0  2
dna update 2003. 8.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9Y1W0) RecName: Full=Delta(5) fatty acid desaturase A;        ...   340   3e-92
(O96099) RecName: Full=Delta(5) fatty acid desaturase B;        ...   237   3e-61
(Q1ZXQ5) RecName: Full=Probable Delta(5) fatty acid desaturase C...   196   6e-49
AF419297_1(AF419297|pid:none) Pythium irregulare delta-5 fatty a...   136   1e-30
AB188307_1(AB188307|pid:none) Mortierella alpina gene for delta5...   134   3e-30
(O74212) RecName: Full=Delta(5) fatty acid desaturase;          ...   132   1e-29
CP000592_278(CP000592|pid:none) Ostreococcus lucimarinus CCE9901...   126   1e-27
CS020137_1(CS020137|pid:none) Sequence 91 from Patent WO20050123...   114   3e-24
EF678404_1(EF678404|pid:none) Picea sitchensis clone WS0291_A04 ...   112   2e-23
AM949596_1(AM949596|pid:none) Mantoniella squamata d5 gene for A...   111   3e-23
protein update 2009. 3.22
PSORT 

 psg: 0.76  gvh: 0.36  alm: 0.33  top: 0.53  tms: 0.00  mit: 0.13  mip: 0.10
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	 20.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VSF803 is cyt
5' end seq. ID VSF803F
5' end seq. 
>VSF803F.Seq
CCACGCCGTCCGACATATATTAATAGAATTGTAATAAAATGTCAAAAGTAATTACAGGAA
AGCAATACTCTTGGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGCAG
TCGATGGTAAAGTTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGAAG
TTTTACTTTTAGCAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAATGT
CAGATAAACCAACATCAATTTTTTAAAGGAATTATGAAATTGGATATATTTCATCATATG
AACATCCAAAATTTGTTCAAAAATCAGATTTCTAC----------
Length of 5' end seq. 335
3' end seq. ID VSF803Z
3' end seq. 
>VSF803Z.Seq
----------TTTGTTTTCTTCCGTTTCATCCTTCCACTCCGTTATCATAGCTTTACAGA
TTTAATTTGTTATTTCCTCATTGCTGAATTCGTCTTTGGTTGGTATCTCACAATTAATTT
CCAAGTTAGTCATGTCGCTGAAGATCTCAAATTCTTTGCTACCCCTGAAAGACCAGATGA
ACCATCTCAAATCAATGAAGATTGGGCAATCCTTCAACTTAAAACTACTCAAGATTATGG
TCATGGTTCACTCCTTTGTACCTTTTTTAGTGGTTCTTTAAATCATCAAGTTGTTCATCA
TTTATTCCCATCAATTGCTCAAGATTTCTACCCACAACTTGTACCAATTGTAAAAGAAGT
TTGTAAAGAACATAACATTACTTACCACATTAAACCAAACTTCACTGAAGCTATTATGTC
ACACATTAATTACCTTTACAAAATGGGTAATGANCCAGATTATGTTAAAAAACCATTAGC
CTCAAAAGATGATTAAATGAAATAACTTAAAAACCAATTATTTACTTGACAAACAGTAAT
Length of 3' end seq. 530
Connected seq. ID VSF803P
Connected seq. 
>VSF803P.Seq
CCACGCCGTCCGACATATATTAATAGAATTGTAATAAAATGTCAAAAGTAATTACAGGAA
AGCAATACTCTTGGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGCAG
TCGATGGTAAAGTTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGAAG
TTTTACTTTTAGCAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAATGT
CAGATAAACCAACATCAATTTTTTAAAGGAATTATGAAATTGGATATATTTCATCATATG
AACATCCAAAATTTGTTCAAAAATCAGATTTCTAC----------TTTGTTTTCTTCCGT
TTCATCCTTCCACTCCGTTATCATAGCTTTACAGATTTAATTTGTTATTTCCTCATTGCT
GAATTCGTCTTTGGTTGGTATCTCACAATTAATTTCCAAGTTAGTCATGTCGCTGAAGAT
CTCAAATTCTTTGCTACCCCTGAAAGACCAGATGAACCATCTCAAATCAATGAAGATTGG
GCAATCCTTCAACTTAAAACTACTCAAGATTATGGTCATGGTTCACTCCTTTGTACCTTT
TTTAGTGGTTCTTTAAATCATCAAGTTGTTCATCATTTATTCCCATCAATTGCTCAAGAT
TTCTACCCACAACTTGTACCAATTGTAAAAGAAGTTTGTAAAGAACATAACATTACTTAC
CACATTAAACCAAACTTCACTGAAGCTATTATGTCACACATTAATTACCTTTACAAAATG
GGTAATGANCCAGATTATGTTAAAAAACCATTAGCCTCAAAAGATGATTAAATGAAATAA
CTTAAAAACCAATTATTTACTTGACAAACAGTAAT
Length of connected seq. 865
Full length Seq ID -
Full length Seq. -
Length of full length seq. -