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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSF637
Library VS
(Link to library)
Clone ID VSF637
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig -
Original site URL
Representative seq. ID VSF637P
(Link to Original site)
Representative DNA sequence 
>VSF637 (VSF637Q) /CSM/VS/VSF6-B/VSF637Q.Seq.d/
AACCACACAAATAAATTAAAATAAAATGACACTCGGAGGATTAAAACCAGAAGTACACGC
CGCCAATGATGAAATTAGACAAGTCGTTGCCAAAGTTGCCGATGAACTCAAATCTAAATT
AAACACCACTGAAGTCGAACCAGTTTCATACAAAACTCAACTCGTTGCTGGTACCAACTA
CTTTATCAAAGTTAAGACCCCAGCTGGTTTTGCTCATGCTCGTGTCTACAAAGATTTACA
ACAAAACCATTCAGTCCACTCCGTCAAAGCTGATGGTATCACCGAAGAATCAGAAATTGT
TTATTTCTAAACTTTCAAGTGTATAATATATAAAACAATTGTTACTTTTATACTAAAATA
AATAAAACACCTAAAAACAAAAAAAAAAXXXXXXXXXXAACCACACAAATAAATTAAAAT
AAAATGACACTCGGAGGATTAAAACCAGAAGTACACGCCGCCAATGATGAAATTAGACAA
GTCGTTGCCAAAGTTGCCGATGAACTCAAATCTAAATTAAACACCACTGAAGTCGAACCA
GTTTCATACAAAACTCAACTCGTTGCTGGTACCAACTACTTTATCAAAGTTAAGACCCCA
GCTGGTTTTGCTCATGCTCGTGTCTACAAAGATTTACAACAAAACCATTCAGTCCACTCC
GTCAAAGCTGATGGTATCACCGAAGAATCAGAAATTGTTTATTTCTAAACTTTCAAGTGT
ATAATATATAAAACAATTGTTACTTTATACTAAAAAAAAAAACACC
sequence update 2001. 3.22
Translated Amino Acid sequence 
nhtnklk*ndtrriktrstrrq**n*tsrcqscr*tqi*ikhh*srtsfiqnstrcwyql
lyqs*dpswfcscsclqrfttkpfsplrqs*wyhrrirnclflnfqvyni*NNCYFYTKI
NKTPKNKKK---

---NHTNKLK*ndtrriktrstrrq**n*tsrcqscr*tqi*ikhh*srtsfiqnstrcw
yqllyqs*dpswfcscsclqrfttkpfsplrqs*wyhrrirnclflnfqvyni*nncyfi
lkkkt
Translated Amino Acid sequence (All Frames) 
Frame A:
nhtnklk*ndtrriktrstrrq**n*tsrcqscr*tqi*ikhh*srtsfiqnstrcwyql
lyqs*dpswfcscsclqrfttkpfsplrqs*wyhrrirnclflnfqvyni*NNCYFYTKI
NKTPKNKKK---

---NHTNKLK*ndtrriktrstrrq**n*tsrcqscr*tqi*ikhh*srtsfiqnstrcw
yqllyqs*dpswfcscsclqrfttkpfsplrqs*wyhrrirnclflnfqvyni*nncyfi
lkkkt

Frame B:
ttqin*nkmtlgglkpevhaandeirqvvakvadelksklnttevepvsyktqlvagtny
fikvktpagfaharvykdlqqnhsvhsvkadgiteeseivyf*tfkciiyktivtfilk*
ikhlktkkk---

---ttqin*nkmtlgglkpevhaandeirqvvakvadelksklnttevepvsyktqlvag
tnyfikvktpagfaharvykdlqqnhsvhsvkadgiteeseivyf*tfkciiyktivtly
*kkkh

Frame C:
phk*ikik*hsed*nqkytppmmkldkslpklpmnsnln*tplksnqfhtklnsllvptt
lsklrpqlvllmlvstkiynktiqstpsklmvspknqklfisklssv*yikqlllly*nk
*nt*kqkk---

---phk*ikik*hsed*nqkytppmmkldkslpklpmnsnln*tplksnqfhtklnsllv
pttlsklrpqlvllmlvstkiynktiqstpsklmvspknqklfisklssv*yikqlllyt
kkknt
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSF637 (VSF637Q) /CSM/VS/VSF6-B/VSF637Q.Seq.d/                        700   0.0
VSE231 (VSE231Q) /CSM/VS/VSE2-B/VSE231Q.Seq.d/                        692   0.0
VHI872 (VHI872Q) /CSM/VH/VHI8-C/VHI872Q.Seq.d/                        692   0.0
VSF605 (VSF605Q) /CSM/VS/VSF6-A/VSF605Q.Seq.d/                        690   0.0
VSF202 (VSF202Q) /CSM/VS/VSF2-A/VSF202Q.Seq.d/                        686   0.0
SSI815 (SSI815Q) /CSM/SS/SSI8-A/SSI815Q.Seq.d/                        686   0.0
VSB135 (VSB135Q) /CSM/VS/VSB1-B/VSB135Q.Seq.d/                        684   0.0
SSK228 (SSK228Q) /CSM/SS/SSK2-B/SSK228Q.Seq.d/                        684   0.0
VSK254 (VSK254Q) /CSM/VS/VSK2-C/VSK254Q.Seq.d/                        682   0.0
VHC585 (VHC585Q) /CSM/VH/VHC5-D/VHC585Q.Seq.d/                        676   0.0
own update 2004. 5.29
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BQ633139|BQ633139.1 PfESToab38c04.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum 3D7 cDNA 5', mRNA sequence.    38  0.002  2
BU496524|BU496524.1 PfESToab51c12.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5', mRNA sequence.    38  0.002  2
BU496772|BU496772.1 PfESToab58f06.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5', mRNA sequence.    38  0.002  2
AX344551|AX344551.1 Sequence 2 from Patent WO0200932.             34  0.002  10
AX251260|AX251260.1 Sequence 228 from Patent WO0168912.           36  0.007  3
M27786|M27786.1 Synthetic human inhibitorily active MS-2 pol-stefin B fusion protein gene, complete cds.    52  0.010  1
BX005206|BX005206.9 Mouse DNA sequence *** SEQUENCING IN PROGRESS *** from clone  RP23-384P3.    38  0.016  3
AE014826|AE014826.1 Plasmodium falciparum 3D7 chromosome 14 section 11 of 13 of the complete sequence.    38  0.017  6
BG601370|BG601370.1 EST500460 Plasmodium yoelii sporozoite cDNA Plasmodium yoelii cDNA clone PYCDB51, mRNA sequence.    36  0.032  2
BG601369|BG601369.1 EST500459 Plasmodium yoelii sporozoite cDNA Plasmodium yoelii cDNA clone PYCDB51, mRNA sequence.    36  0.041  2
dna update 2003. 8.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q65YR8) RecName: Full=Cystatin-A1;  &AB189918_1(AB189918|pid:no...   187   2e-46
(Q65YR7) RecName: Full=Cystatin-A2;  &AB189919_1(AB189919|pid:no...    73   1e-11
(Q5R1U3) RecName: Full=Cystatin-A3; AltName: Full=VSA745;  &AB18...    64   5e-09
AC116987_9(AC116987|pid:none) Dictyostelium discoideum chromosom...    60   7e-08
EU979494_1(EU979494|pid:none) Ornithoctonus huwena clone HWEc85 ...    57   5e-07
(P01040) RecName: Full=Cystatin-A; AltName: Full=Cystatin-AS; Al...    57   5e-07
DQ646915_1(DQ646915|pid:none) Boophilus microplus putative intra...    55   2e-06
BC146444_1(BC146444|pid:none) Synthetic construct Mus musculus c...    54   7e-06
BC166959_1(BC166959|pid:none) Xenopus tropicalis cDNA clone MGC:...    53   1e-05
(P80416) RecName: Full=Cystatin-A; AltName: Full=Cystatin-AS; Al...    52   1e-05
protein update 2009. 3.22
PSORT 

 psg: 0.30  gvh: -0.07  alm: 0.87  top: 0.53  tms: 0.00  mit: 0.39  mip: 0.00
 nuc: 0.30  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: nuclear
	 24.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for VSF637 is nuc
5' end seq. ID VSF637F
5' end seq. 
>VSF637F.Seq
AACCACACAAATAAATTAAAATAAAATGACACTCGGAGGATTAAAACCAGAAGTACACGC
CGCCAATGATGAAATTAGACAAGTCGTTGCCAAAGTTGCCGATGAACTCAAATCTAAATT
AAACACCACTGAAGTCGAACCAGTTTCATACAAAACTCAACTCGTTGCTGGTACCAACTA
CTTTATCAAAGTTAAGACCCCAGCTGGTTTTGCTCATGCTCGTGTCTACAAAGATTTACA
ACAAAACCATTCAGTCCACTCCGTCAAAGCTGATGGTATCACCGAAGAATCAGAAATTGT
TTATTTCTAAACTTTCAAGTGTATAATATATAAAACAATTGTTACTTTTATACTAAAATA
AATAAAACACCTAAAAACAAAAAAAAAA----------
Length of 5' end seq. 388
3' end seq. ID VSF637Z
3' end seq. 
>VSF637Z.Seq
----------AACCACACAAATAAATTAAAATAAAATGACACTCGGAGGATTAAAACCAG
AAGTACACGCCGCCAATGATGAAATTAGACAAGTCGTTGCCAAAGTTGCCGATGAACTCA
AATCTAAATTAAACACCACTGAAGTCGAACCAGTTTCATACAAAACTCAACTCGTTGCTG
GTACCAACTACTTTATCAAAGTTAAGACCCCAGCTGGTTTTGCTCATGCTCGTGTCTACA
AAGATTTACAACAAAACCATTCAGTCCACTCCGTCAAAGCTGATGGTATCACCGAAGAAT
CAGAAATTGTTTATTTCTAAACTTTCAAGTGTATAATATATAAAACAATTGTTACTTTAT
ACTAAAAAAAAAAACACC
Length of 3' end seq. 368
Connected seq. ID VSF637P
Connected seq. 
>VSF637P.Seq
AACCACACAAATAAATTAAAATAAAATGACACTCGGAGGATTAAAACCAGAAGTACACGC
CGCCAATGATGAAATTAGACAAGTCGTTGCCAAAGTTGCCGATGAACTCAAATCTAAATT
AAACACCACTGAAGTCGAACCAGTTTCATACAAAACTCAACTCGTTGCTGGTACCAACTA
CTTTATCAAAGTTAAGACCCCAGCTGGTTTTGCTCATGCTCGTGTCTACAAAGATTTACA
ACAAAACCATTCAGTCCACTCCGTCAAAGCTGATGGTATCACCGAAGAATCAGAAATTGT
TTATTTCTAAACTTTCAAGTGTATAATATATAAAACAATTGTTACTTTTATACTAAAATA
AATAAAACACCTAAAAACAAAAAAAAAA----------AACCACACAAATAAATTAAAAT
AAAATGACACTCGGAGGATTAAAACCAGAAGTACACGCCGCCAATGATGAAATTAGACAA
GTCGTTGCCAAAGTTGCCGATGAACTCAAATCTAAATTAAACACCACTGAAGTCGAACCA
GTTTCATACAAAACTCAACTCGTTGCTGGTACCAACTACTTTATCAAAGTTAAGACCCCA
GCTGGTTTTGCTCATGCTCGTGTCTACAAAGATTTACAACAAAACCATTCAGTCCACTCC
GTCAAAGCTGATGGTATCACCGAAGAATCAGAAATTGTTTATTTCTAAACTTTCAAGTGT
ATAATATATAAAACAATTGTTACTTTATACTAAAAAAAAAAACACC
Length of connected seq. 756
Full length Seq ID -
Full length Seq. -
Length of full length seq. -