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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSF418
Library VS
(Link to library)
Clone ID VSF418
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15178-1
Original site URL
Representative seq. ID VSF418P
(Link to Original site)
Representative DNA sequence 
>VSF418 (VSF418Q) /CSM/VS/VSF4-A/VSF418Q.Seq.d/
AAAATGACAGGAAAAGTTGTAGTTAAAACCACTAATGCACCAGGTGCTATTGGCCCATAC
AGTCAAGCAATTATTGCTAACAATCAAGTTTTCGTTTCTGGTTGTTTAGGTATTGATAAA
GATACTATGCAATTCACATCTGAAACTGATGTTAGTATTCAAACTAAACTTGCTTTAACA
AACATGAAAAACATTGTTGAAGCTGCTGGTTCATCAATGGAAAAAGTTGTTAAAACTACC
ATCTTATTAAAATCAATGGATGATTTCCAAGCTGTTAACACTGTCTATTCTACTTTCTTC
CCAGTTGACCCACCAGCTAGATCAACCTTTGCTGTTGCTTGTTTACCAAAGAATGCTTTA
GTAGAAATTGAAGCTATTGCAATTCACCAATGAACCGAGGGTAAATCAAATAATATCATC
AATTCAAAATTATAAATAATAAATAATAAGTAATAGTTTATTCTATTATTGTAAAAAAGA
ACTAAAATATAATAATAGTTTTTATTTTGTTTCCAAATATAAAAAAAAAAXXXXXXXXXX
AAAATGACAGGAAAAGTTGTAGTTAAAACCACTAATGCACCAGGTGCTATTGGCCCATAC
AGTCAAGCAATTATTGCTAACAATCAAGTTTTCGTTTCTGGTTGTTTAGGTATTGATAAA
GATACTATGCAATTCACATCTGAAACTGATGTTAGTATTCAAACTAAACTTGCTTTAACA
AACATGAAAAACATTGTTGAAGCTGCTGGTTCATCAATGGAAAAAGTTGTTAAAACTACC
ATCTTATTAAAATCAATGGATGATTTCCAAGCTGTTAACACTGTCTATTCTACTTTCTTC
CCAGTTGACCCACCAGCTAGATCAACCTTTGCTGTTGCTTGTTTACCAAAGAATGCTTTA
GTAGAAATTGAAGCTATTGCAATTCACCAATGAACCGAGGGTAAATCAAATAATATCATC
AATTCAAAATTATAAATAATAAATAATAAGNAATAGTTTATTNCTATTATTGTAAAAAAG
CAACTCAAATATAATAATAG
sequence update 2001. 3.22
Translated Amino Acid sequence 
k*qekl*lkplmhqvllahtvkqllltikfsflvv*vlikilcnshlklmlvfklnll*q
t*ktllkllvhqwkkllklpsy*nqwmisklltlsillssqlthqldqpllllvyqrml*
*klkllqftneprvnqiissiqnyk**IISNSLFYYCKKELKYNNSFYFVSKYKKK---

---KMTGKVVVKTTNAPGAIGPYSQAIIANNQVFVSGCLGIDKDTMQFTSETDVSIQTKL
ALTNMKNIVEAAGSSMEKVVKTTILLKSMDDFQAVNTVYSTFFPVDPPARSTFAVACLPK
NALVEIEAIAIHQ*tegksnniinskl*iinnkx*fixiivkkqlkynn
Translated Amino Acid sequence (All Frames) 
Frame A:
kmtgkvvvkttnapgaigpysqaiiannqvfvsgclgidkdtmqftsetdvsiqtklalt
nmkniveaagssmekvvkttillksmddfqavntvystffpvdpparstfavaclpknal
veieaiaihq*tegksnniinskl*iinnk**filll*krtki***flfcfqi*kk---

---KMTGKVVVKTTNAPGAIGPYSQAIIANNQVFVSGCLGIDKDTMQFTSETDVSIQTKL
ALTNMKNIVEAAGSSMEKVVKTTILLKSMDDFQAVNTVYSTFFPVDPPARSTFAVACLPK
NALVEIEAIAIHQ*tegksnniinskl*iinnkx*fixiivkkqlkynn

Frame B:
k*qekl*lkplmhqvllahtvkqllltikfsflvv*vlikilcnshlklmlvfklnll*q
t*ktllkllvhqwkkllklpsy*nqwmisklltlsillssqlthqldqpllllvyqrml*
*klkllqftneprvnqiissiqnyk**IISNSLFYYCKKELKYNNSFYFVSKYKKK---

---k*qekl*lkplmhqvllahtvkqllltikfsflvv*vlikilcnshlklmlvfklnl
l*qt*ktllkllvhqwkkllklpsy*nqwmisklltlsillssqlthqldqpllllvyqr
ml**klkllqftneprvnqiissiqnyk**iixnslxlll*ksnsniii

Frame C:
ndrkscs*nh*ctrcywpiqssnyc*qssfrfwlfry**ryyaihi*n*c*ysn*tcfnk
hekhc*scwfingksc*nyhliking*fpsc*hclfyflps*pts*inlccclftkecfs
rn*sycnspmnrg*ik*yhqfkiinnk**vivysiivkkn*niiivfilfpnikkk---

---ndrkscs*nh*ctrcywpiqssnyc*qssfrfwlfry**ryyaihi*n*c*ysn*tc
fnkhekhc*scwfingksc*nyhliking*fpsc*hclfyflps*pts*inlccclftke
cfsrn*sycnspmnrg*ik*yhqfkiinnk**xivyxyyckkatqi***
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSF418 (VSF418Q) /CSM/VS/VSF4-A/VSF418Q.Seq.d/                        803   0.0
VFN414 (VFN414Q) /CSM/VF/VFN4-A/VFN414Q.Seq.d/                        803   0.0
SSC695 (SSC695Q) /CSM/SS/SSC6-D/SSC695Q.Seq.d/                        803   0.0
SHA718 (SHA718Q) /CSM/SH/SHA7-A/SHA718Q.Seq.d/                        803   0.0
AFM381 (AFM381Q) /CSM/AF/AFM3-D/AFM381Q.Seq.d/                        803   0.0
VSI115 (VSI115Q) /CSM/VS/VSI1-A/VSI115Q.Seq.d/                        789   0.0
FC-BE13 (FC-BE13Q) /CSM/FC/FC-BE/FC-BE13Q.Seq.d/                      779   0.0
VSH656 (VSH656Q) /CSM/VS/VSH6-C/VSH656Q.Seq.d/                        735   0.0
VSD732 (VSD732Q) /CSM/VS/VSD7-B/VSD732Q.Seq.d/                        585   e-166
SSF115 (SSF115Q) /CSM/SS/SSF1-A/SSF115Q.Seq.d/                         40   0.066
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC115612|AC115612.2 Dictyostelium discoideum chromosome 2 map 6245135-6357017 strain AX4, complete sequence.   341  0.0  9
BI701710|BI701710.1 sai19b12.y1 Gm-c1053 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1053-3551 5', mRNA sequence.    50  0.056  1
AL158031|AL158031.11 Human DNA sequence *** SEQUENCING CANCELLED *** from clone  RP11-92B21.    36  0.31  3
AC116920|AC116920.2 Dictyostelium discoideum chromosome 2 map 3879572-4071762 strain AX4, complete sequence.    44  0.48  7
AL672288|AL672288.5 Mouse DNA sequence from clone RP23-186F4 on chromosome X.    42  0.74  4
AC015634|AC015634.2 Homo sapiens clone RP11-45L24, LOW-PASS SEQUENCE SAMPLING.    42  0.85  3
AQ230683|AQ230683.1 HS_2033_B2_B06_MR CIT Approved Human Genomic Sperm Library D Homo sapiens genomic clone Plate=2033 Col=12 Row=D, genomic survey sequence.    46  0.88  1
CC233496|CC233496.1 CH261-183N21_RM1.1 CH261 Gallus gallus genomic clone CH261-183N21, DNA sequence.    46  0.88  1
AC136634|AC136634.2 Homo sapiens chromosome 16 clone RP11-95C20, complete sequence.    46  0.88  1
BZ610829|BZ610829.1 WHACT95TF Human MCF7 breast cancer cell line library (MCF7_1) Homo sapiens genomic clone MCF7_1-17P22, DNA sequence.    46  0.88  1
dna update 2003. 8.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP001072_955(CP001072|pid:none) Helicobacter pylori Shi470, comp...   132   1e-29
AP008230_1335(AP008230|pid:none) Desulfitobacterium hafniense Y5...   132   2e-29
CP000241_928(CP000241|pid:none) Helicobacter pylori HPAG1, compl...   131   4e-29
(O25598) UPF0076 protein HP_0944.  &AE000511_928(AE000511|pid:no...   130   9e-29
AM260522_1180(AM260522|pid:none) Helicobacter acinonychis str. S...   128   3e-28
AK061373_1(AK061373|pid:none) Oryza sativa Japonica Group cDNA c...   127   5e-28
CP000686_2882(CP000686|pid:none) Roseiflexus sp. RS-1, complete ...   127   8e-28
BT039439_1(BT039439|pid:none) Zea mays full-length cDNA clone ZM...   126   1e-27
CT573071_938(CT573071|pid:none) Kuenenia stuttgartiensis genome ...   125   2e-27
CP000804_1890(CP000804|pid:none) Roseiflexus castenholzii DSM 13...   125   3e-27
protein update 2009. 3.22
PSORT 

 psg: 0.42  gvh: 0.42  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 32.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VSF418 is nuc
5' end seq. ID VSF418F
5' end seq. 
>VSF418F.Seq
AAAATGACAGGAAAAGTTGTAGTTAAAACCACTAATGCACCAGGTGCTATTGGCCCATAC
AGTCAAGCAATTATTGCTAACAATCAAGTTTTCGTTTCTGGTTGTTTAGGTATTGATAAA
GATACTATGCAATTCACATCTGAAACTGATGTTAGTATTCAAACTAAACTTGCTTTAACA
AACATGAAAAACATTGTTGAAGCTGCTGGTTCATCAATGGAAAAAGTTGTTAAAACTACC
ATCTTATTAAAATCAATGGATGATTTCCAAGCTGTTAACACTGTCTATTCTACTTTCTTC
CCAGTTGACCCACCAGCTAGATCAACCTTTGCTGTTGCTTGTTTACCAAAGAATGCTTTA
GTAGAAATTGAAGCTATTGCAATTCACCAATGAACCGAGGGTAAATCAAATAATATCATC
AATTCAAAATTATAAATAATAAATAATAAGTAATAGTTTATTCTATTATTGTAAAAAAGA
ACTAAAATATAATAATAGTTTTTATTTTGTTTCCAAATATAAAAAAAAAA----------
Length of 5' end seq. 530
3' end seq. ID VSF418Z
3' end seq. 
>VSF418Z.Seq
----------AAAATGACAGGAAAAGTTGTAGTTAAAACCACTAATGCACCAGGTGCTAT
TGGCCCATACAGTCAAGCAATTATTGCTAACAATCAAGTTTTCGTTTCTGGTTGTTTAGG
TATTGATAAAGATACTATGCAATTCACATCTGAAACTGATGTTAGTATTCAAACTAAACT
TGCTTTAACAAACATGAAAAACATTGTTGAAGCTGCTGGTTCATCAATGGAAAAAGTTGT
TAAAACTACCATCTTATTAAAATCAATGGATGATTTCCAAGCTGTTAACACTGTCTATTC
TACTTTCTTCCCAGTTGACCCACCAGCTAGATCAACCTTTGCTGTTGCTTGTTTACCAAA
GAATGCTTTAGTAGAAATTGAAGCTATTGCAATTCACCAATGAACCGAGGGTAAATCAAA
TAATATCATCAATTCAAAATTATAAATAATAAATAATAAGNAATAGTTTATTNCTATTAT
TGTAAAAAAGCAACTCAAATATAATAATAG
Length of 3' end seq. 500
Connected seq. ID VSF418P
Connected seq. 
>VSF418P.Seq
AAAATGACAGGAAAAGTTGTAGTTAAAACCACTAATGCACCAGGTGCTATTGGCCCATAC
AGTCAAGCAATTATTGCTAACAATCAAGTTTTCGTTTCTGGTTGTTTAGGTATTGATAAA
GATACTATGCAATTCACATCTGAAACTGATGTTAGTATTCAAACTAAACTTGCTTTAACA
AACATGAAAAACATTGTTGAAGCTGCTGGTTCATCAATGGAAAAAGTTGTTAAAACTACC
ATCTTATTAAAATCAATGGATGATTTCCAAGCTGTTAACACTGTCTATTCTACTTTCTTC
CCAGTTGACCCACCAGCTAGATCAACCTTTGCTGTTGCTTGTTTACCAAAGAATGCTTTA
GTAGAAATTGAAGCTATTGCAATTCACCAATGAACCGAGGGTAAATCAAATAATATCATC
AATTCAAAATTATAAATAATAAATAATAAGTAATAGTTTATTCTATTATTGTAAAAAAGA
ACTAAAATATAATAATAGTTTTTATTTTGTTTCCAAATATAAAAAAAAAA----------
AAAATGACAGGAAAAGTTGTAGTTAAAACCACTAATGCACCAGGTGCTATTGGCCCATAC
AGTCAAGCAATTATTGCTAACAATCAAGTTTTCGTTTCTGGTTGTTTAGGTATTGATAAA
GATACTATGCAATTCACATCTGAAACTGATGTTAGTATTCAAACTAAACTTGCTTTAACA
AACATGAAAAACATTGTTGAAGCTGCTGGTTCATCAATGGAAAAAGTTGTTAAAACTACC
ATCTTATTAAAATCAATGGATGATTTCCAAGCTGTTAACACTGTCTATTCTACTTTCTTC
CCAGTTGACCCACCAGCTAGATCAACCTTTGCTGTTGCTTGTTTACCAAAGAATGCTTTA
GTAGAAATTGAAGCTATTGCAATTCACCAATGAACCGAGGGTAAATCAAATAATATCATC
AATTCAAAATTATAAATAATAAATAATAAGNAATAGTTTATTNCTATTATTGTAAAAAAG
CAACTCAAATATAATAATAG
Length of connected seq. 1030
Full length Seq ID -
Full length Seq. -
Length of full length seq. -