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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSF284
Library VS
(Link to library)
Clone ID VSF284
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13504-1
Original site URL
Representative seq. ID VSF284P
(Link to Original site)
Representative DNA sequence 
>VSF284 (VSF284Q) /CSM/VS/VSF2-D/VSF284Q.Seq.d/
CGACCCACGCGTCCGCCCACGCGTNCGCXXXXXXXXXXCGTCCGCACATTACCATCACCC
ACAATGTAAAATCACCCAAGCTACTTGGGGTCCATTAAATAAAACTATTTTTGCTTCATG
TGAAGATGGTGCCGTTCGTATTTATTGCACAGAGAAAAGAGAACTCATTAAAACTATCCT
TGATCACAACAAATTAGTCACAAGAATTGAATGGACTAAACATAGAATTATGTTTATGAC
TTGTTCAAAAGATGGTACTGCTAAATTATATGACACAAAAACATTAAAACTCTTAAGAAC
TTTCGATACTGGTCGTCCAATTAATGCAGCTGGTATTTCACCACTTAAACCACACGTTAT
TCTTGGTGGTGGTCAATCAGCTGAATCTGTAACCACAACCAAAGTAGATGCCTCTCAATT
CAAAGTTAGATTTTTCCATATTGTTTATGGTGAAGAATTAGGTGGTTTAATTGGTCATAT
TGGTCCAGTTCACTCAATTTGTTTCACTCCAGATGGTAAAACATTTGCAACTGGTGGTGA
AGAAGGTCTTGTTCAAGTTAATCATTTGGATGAATCATATTTCGAATTTGATGACGATTT
AGTTTATAACCCACCAGTTCAACATTAAAATATAATAAGGGAGAAGAAGCGTATAAATAG
GTTGCACAAAA
sequence update 2001. 3.22
Translated Amino Acid sequence 
RPTRPPTRX---

---SAHYHHPQCKITQATWGPLNKTIFASCEDGAVRIYCTEKRELIKTILDHNKLVTRIE
WTKHRIMFMTCSKDGTAKLYDTKTLKLLRTFDTGRPINAAGISPLKPHVILGGGQSAESV
TTTKVDASQFKVRFFHIVYGEELGGLIGHIGPVHSICFTPDGKTFATGGEEGLVQVNHLD
ESYFEFDDDLVYNPPVQH*niirekkrinrlhk
Translated Amino Acid sequence (All Frames) 
Frame A:
RPTRPPTRX---

---rphitithnvkspkllgvh*iklfllhvkmvpfvfiaqrkenslklslittn*sqel
nglnielcl*lvqkmvllnymtqkh*ns*elsilvvqlmqlvfhhlnhtlflvvvnqlnl
*pqpk*mplnskldfsilfmvkn*vv*lvilvqftqfvslqmvkhlqlvvkkvlfkliiw
mnhisnlmti*fithqfniki**grrsv*igctk

Frame B:
dprvrprvr---

---vrtlpsptm*nhpsylgsik*nyfcfm*rwcrsyllhrekrth*nyp*sqqishkn*
md*t*nyvydlfkrwyc*ii*hkniktlknfrywssn*cswyftt*ttryswwwsis*ic
nhnqsrclsiqs*ifpyclw*rirwfnwsywssslnlfhsrw*nicnww*rrscss*sfg
*iifri**rfsl*ptsstlkynkgeeayk*vaq

Frame C:
thasahax---

---SAHYHHPQCKITQATWGPLNKTIFASCEDGAVRIYCTEKRELIKTILDHNKLVTRIE
WTKHRIMFMTCSKDGTAKLYDTKTLKLLRTFDTGRPINAAGISPLKPHVILGGGQSAESV
TTTKVDASQFKVRFFHIVYGEELGGLIGHIGPVHSICFTPDGKTFATGGEEGLVQVNHLD
ESYFEFDDDLVYNPPVQH*niirekkrinrlhk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSF284 (VSF284Q) /CSM/VS/VSF2-D/VSF284Q.Seq.d/                       1265   0.0
CHD850 (CHD850Q) /CSM/CH/CHD8-C/CHD850Q.Seq.d/                       1191   0.0
VFB170 (VFB170Q) /CSM/VF/VFB1-C/VFB170Q.Seq.d/                       1170   0.0
VFB661 (VFB661Q) /CSM/VF/VFB6-C/VFB661Q.Seq.d/                       1166   0.0
VFB682 (VFB682Q) /CSM/VF/VFB6-D/VFB682Q.Seq.d/                       1150   0.0
VFC278 (VFC278Q) /CSM/VF/VFC2-D/VFC278Q.Seq.d/                       1130   0.0
AHG631 (AHG631Q) /CSM/AH/AHG6-B/AHG631Q.Seq.d/                       1128   0.0
VFK135 (VFK135Q) /CSM/VF/VFK1-B/VFK135Q.Seq.d/                       1116   0.0
VSF253 (VSF253Q) /CSM/VS/VSF2-C/VSF253Q.Seq.d/                       1114   0.0
VFJ162 (VFJ162Q) /CSM/VF/VFJ1-C/VFJ162Q.Seq.d/                       1106   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BM605098|BM605098.1 17000687075777 A.Gam.ad.cDNA.blood1 Anopheles gambiae cDNA clone 19600449716696 5', mRNA sequence.    50  5e-05  2
BI217776|BI217776.1 602934636F1 NCI_CGAP_Li9 Mus musculus cDNA clone IMAGE:5098017 5', mRNA sequence.    50  7e-04  2
BG787079|BG787079.1 SEAUMC007036 Sea urchin primary mesenchyme cell cDNA library Strongylocentrotus purpuratus cDNA clone PM990802-02-0482 5', mRNA sequence.    48  0.001  2
BM602053|BM602053.1 17000687068891 A.Gam.ad.cDNA.blood1 Anopheles gambiae cDNA clone 19600449722729 5', mRNA sequence.    50  0.001  2
BD223450|BD223450.1 94 human secretory proteins.                  50  0.001  2
AR243084|AR243084.1 Sequence 54 from patent US 6475753.           50  0.001  2
AW872084|AW872084.1 db22b05.y1 Xenopus laevis oocyte Xenopus laevis cDNA clone XENOPUS_SOURCE_ID: 5' similar to gb:Z23064_cds1 HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN G (HUMAN);, mRNA sequence.    50  0.002  2
AW872134|AW872134.1 db22f08.y1 Xenopus laevis oocyte Xenopus laevis cDNA clone XENOPUS_SOURCE_ID: 5' similar to gb:S66431 RETINOBLASTOMA BINDING PROTEIN 2 (HUMAN);, mRNA sequence.    50  0.002  2
CB104516|CB104516.1 ADP_SQ021@C12 AD-wrmcDNA Caenorhabditis elegans cDNA, mRNA sequence.    50  0.002  2
CB104290|CB104290.1 ADP_SQ018@D2 AD-wrmcDNA Caenorhabditis elegans cDNA, mRNA sequence.    50  0.002  2
dna update 2003. 8.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54MT0) RecName: Full=Eukaryotic translation initiation factor ...   366   e-100
(B5FZ19) RecName: Full=Eukaryotic translation initiation factor ...   161   1e-38
(Q66J51) RecName: Full=Eukaryotic translation initiation factor ...   161   2e-38
(B4N0L0) RecName: Full=Eukaryotic translation initiation factor ...   159   6e-38
AB173142_1(AB173142|pid:none) Macaca fascicularis brain cDNA clo...   159   6e-38
(Q5E966) RecName: Full=Eukaryotic translation initiation factor ...   159   9e-38
(Q9QZD9) RecName: Full=Eukaryotic translation initiation factor ...   159   9e-38
(B0BNA7) RecName: Full=Eukaryotic translation initiation factor ...   157   2e-37
AY888456_1(AY888456|pid:none) Synthetic construct Homo sapiens c...   157   3e-37
(Q16K15) RecName: Full=Eukaryotic translation initiation factor ...   157   4e-37
protein update 2009. 3.22
PSORT 

 psg: 0.67  gvh: 0.26  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.51  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: mitochondrial
	 16.0 %: cytoplasmic
	 12.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane
	  4.0 %: endoplasmic reticulum

>> prediction for VSF284 is mit
5' end seq. ID VSF284F
5' end seq. 
>VSF284F.Seq
CGACCCACGCGTCCGCCCACGCGTNCGC----------
Length of 5' end seq. 28
3' end seq. ID VSF284Z
3' end seq. 
>VSF284Z.Seq
----------CGTCCGCACATTACCATCACCCACAATGTAAAATCACCCAAGCTACTTGG
GGTCCATTAAATAAAACTATTTTTGCTTCATGTGAAGATGGTGCCGTTCGTATTTATTGC
ACAGAGAAAAGAGAACTCATTAAAACTATCCTTGATCACAACAAATTAGTCACAAGAATT
GAATGGACTAAACATAGAATTATGTTTATGACTTGTTCAAAAGATGGTACTGCTAAATTA
TATGACACAAAAACATTAAAACTCTTAAGAACTTTCGATACTGGTCGTCCAATTAATGCA
GCTGGTATTTCACCACTTAAACCACACGTTATTCTTGGTGGTGGTCAATCAGCTGAATCT
GTAACCACAACCAAAGTAGATGCCTCTCAATTCAAAGTTAGATTTTTCCATATTGTTTAT
GGTGAAGAATTAGGTGGTTTAATTGGTCATATTGGTCCAGTTCACTCAATTTGTTTCACT
CCAGATGGTAAAACATTTGCAACTGGTGGTGAAGAAGGTCTTGTTCAAGTTAATCATTTG
GATGAATCATATTTCGAATTTGATGACGATTTAGTTTATAACCCACCAGTTCAACATTAA
AATATAATAAGGGAGAAGAAGCGTATAAATAGGTTGCACAAAA
Length of 3' end seq. 633
Connected seq. ID VSF284P
Connected seq. 
>VSF284P.Seq
CGACCCACGCGTCCGCCCACGCGTNCGC----------CGTCCGCACATTACCATCACCC
ACAATGTAAAATCACCCAAGCTACTTGGGGTCCATTAAATAAAACTATTTTTGCTTCATG
TGAAGATGGTGCCGTTCGTATTTATTGCACAGAGAAAAGAGAACTCATTAAAACTATCCT
TGATCACAACAAATTAGTCACAAGAATTGAATGGACTAAACATAGAATTATGTTTATGAC
TTGTTCAAAAGATGGTACTGCTAAATTATATGACACAAAAACATTAAAACTCTTAAGAAC
TTTCGATACTGGTCGTCCAATTAATGCAGCTGGTATTTCACCACTTAAACCACACGTTAT
TCTTGGTGGTGGTCAATCAGCTGAATCTGTAACCACAACCAAAGTAGATGCCTCTCAATT
CAAAGTTAGATTTTTCCATATTGTTTATGGTGAAGAATTAGGTGGTTTAATTGGTCATAT
TGGTCCAGTTCACTCAATTTGTTTCACTCCAGATGGTAAAACATTTGCAACTGGTGGTGA
AGAAGGTCTTGTTCAAGTTAATCATTTGGATGAATCATATTTCGAATTTGATGACGATTT
AGTTTATAACCCACCAGTTCAACATTAAAATATAATAAGGGAGAAGAAGCGTATAAATAG
GTTGCACAAAA
Length of connected seq. 661
Full length Seq ID -
Full length Seq. -
Length of full length seq. -