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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSF275
Library VS
(Link to library)
Clone ID VSF275
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VSF275P
(Link to Original site)
Representative DNA sequence 
>VSF275 (VSF275Q) /CSM/VS/VSF2-D/VSF275Q.Seq.d/
ATGCCAAACGTCCAAAAAAACCGTCGTAAAGAAATCTCATAAATTCGTCATTGATTGTAC
CGCTCCAGCTGGTAAAATCGTTGATGTTGCTGCTTTCGAAAAGTACTTACACGACAGAAT
CAAAGTTGACAACAAAGTTAGTAACCTCGGTTCAAATGTTGTTATCTCAAAAGATAAATC
AAAGATCATCATCAACACCACCATCCCATTCTCAAAGAGATACCTCAAATATTTAACAAA
GAAATTCTTAAAATTCAAACAAATCAGAGATTTCTTACGTGTTGTTGCCACCACCAAAAA
TACATACGAACTCAGATACTTCAACATTGGTGATTCTGAATCACAAGAATAAATTAATTT
TAATTTTTAATTTATTTTTTTTAAAAAAAAAAXXXXXXXXXXATGCCAAACGTCCAAAAA
AACCGTCGTAAAGAAATCTCATAAATTCGTCATTGATTGTACCGCTCCAGCTGGTAAAAT
CGTTGATGTTGCTGCTTTCGAAAAGTACTTACACGACAGAATCAAAGTTGACAACAAAGT
TAGTAACCTCGGTTCAAATGTTGTTATCTCAAAAGATAAATCAAAGATCATCATCAACAC
CACCATCCCATTCTCAAAGAGATACCTCAAATATTTAACAAAGAAATTCTTAAAATTCAA
ACAAATCAGAGATTTCTTACGTGTTGTTGCCACCACCAAAAATACATACGAACTCAGATA
CTTCAACATTGGGATTCTGAATCACAAGAATAAATTAATTTTAATTTTAA
sequence update 2001. 3.22
Translated Amino Acid sequence 
akrpkkps*rnlinsslivplqlvkslmlllskstyttesklttklvtsvqmllsqkinq
rssstppshsqrdtsni*qrns*NSNKSEISYVLLPPPKIHTNSDTSTLVILNHKNKLIL
IFNLFFLKKK---

---CQTSKKTVVKKSHKFVIDCTAPAGKIVDVAAFEKYLHDRIKVDNKVSNLGSNVVISK
DKSKIIINTTIPFSKRYLKYLTKKFLKFKQIRDFLRVVATTKNTYELRYFNIGILNHKNK
LILIL
Translated Amino Acid sequence (All Frames) 
Frame A:
mpnvqknrrkeis*irh*lyrssw*nr*cccfrkvltrqnqs*qqs**prfkccylkr*i
kdhhqhhhpilkeipqifnkeilkiqtnqrfltccchhqkyirtqilqhw*f*itrin*f
*fliyff*kk---

---mpnvqknrrkeis*irh*lyrssw*nr*cccfrkvltrqnqs*qqs**prfkccylk
r*ikdhhqhhhpilkeipqifnkeilkiqtnqrfltccchhqkyirtqilqhwdsesqe*
infnf

Frame B:
cqtskktvvkkshkfvidctapagkivdvaafekylhdrikvdnkvsnlgsnvviskdks
kiiinttipfskrylkyltkkflkfkqirdflrvvattkntyelryfnigdsesqe*inf
nf*fiffkkk---

---CQTSKKTVVKKSHKFVIDCTAPAGKIVDVAAFEKYLHDRIKVDNKVSNLGSNVVISK
DKSKIIINTTIPFSKRYLKYLTKKFLKFKQIRDFLRVVATTKNTYELRYFNIGILNHKNK
LILIL

Frame C:
akrpkkps*rnlinsslivplqlvkslmlllskstyttesklttklvtsvqmllsqkinq
rssstppshsqrdtsni*qrns*NSNKSEISYVLLPPPKIHTNSDTSTLVILNHKNKLIL
IFNLFFLKKK---

---akrpkkps*rnlinsslivplqlvkslmlllskstyttesklttklvtsvqmllsqk
inqrssstppshsqrdtsni*qrns*nsnkseisyvllpppkihtnsdtstlgf*itrin
*f*f*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ227 (VSJ227Q) /CSM/VS/VSJ2-B/VSJ227Q.Seq.d/                        718   0.0
VSF275 (VSF275Q) /CSM/VS/VSF2-D/VSF275Q.Seq.d/                        718   0.0
VSB760 (VSB760Q) /CSM/VS/VSB7-C/VSB760Q.Seq.d/                        718   0.0
VFE444 (VFE444Q) /CSM/VF/VFE4-B/VFE444Q.Seq.d/                        718   0.0
VSJ148 (VSJ148Q) /CSM/VS/VSJ1-B/VSJ148Q.Seq.d/                        573   e-162
VSI831 (VSI831Q) /CSM/VS/VSI8-B/VSI831Q.Seq.d/                        573   e-162
VSG473 (VSG473Q) /CSM/VS/VSG4-D/VSG473Q.Seq.d/                        573   e-162
VSF864 (VSF864Q) /CSM/VS/VSF8-C/VSF864Q.Seq.d/                        573   e-162
VSF652 (VSF652Q) /CSM/VS/VSF6-C/VSF652Q.Seq.d/                        573   e-162
FC-AT11 (FC-AT11Q) /CSM/FC/FC-AT/FC-AT11Q.Seq.d/                      573   e-162
own update 2004. 5.29
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.    44  4e-06  8
AC084345|AC084345.1 Homo sapiens chromosome 8 clone RP5-295D7 map 8, LOW-PASS SEQUENCE SAMPLING.    58  2e-04  1
AC079015|AC079015.8 Homo sapiens chromosome 8, clone RP11-489O18, complete sequence.    56  2e-04  3
AL512446|AL512446.8 Human DNA sequence from clone RP11-268K9 on chromosome 6.    46  6e-04  5
Z81464|Z81464.1 Caenorhabditis elegans cosmid C08E8.              46  6e-04  4
AF133300|AF133300.2 Mus musculus MOR 3'Beta1, MOR 3'Beta2, MOR 3'Beta3, and MOR 3'Beta4 genes, complete cds; Cbx3 pseudogene, complete sequence; and MOR 3'Beta5 and MOR 3'Beta6 genes, complete cds.    54  0.001  3
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    48  0.001  6
AC142052|AC142052.1 Rattus norvegicus clone CH230-175D14, *** SEQUENCING IN PROGRESS ***, 55 unordered pieces.    54  0.003  1
BZ164125|BZ164125.1 CH230-328B1.TV CHORI-230 Segment 2 Rattus norvegicus genomic clone CH230-328B1, DNA sequence.    54  0.003  1
AC079201|AC079201.2 Homo sapiens chromosome 8 clone RP11-1080K22 map 8, WORKING DRAFT SEQUENCE, 29 unordered pieces.    56  0.003  2
dna update 2003. 8.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54GK6) RecName: Full=60S ribosomal protein L22 2;                   133   5e-30
(Q54JE3) RecName: Full=60S ribosomal protein L22 1;                   132   2e-29
AF542529_8(AF542529|pid:none) Cryptococcus neoformans var. grubi...    79   2e-13
AF542530_16(AF542530|pid:none) Cryptococcus neoformans var. neof...    79   2e-13
AY710429_17(AY710429|pid:none) Cryptococcus gattii strain E566 M...    77   5e-13
AY710430_6(AY710430|pid:none) Cryptococcus gattii strain WM276 M...    76   1e-12
AF548320_1(AF548320|pid:none) Branchiostoma belcheri ribosomal p...    75   2e-12
AM270020_3(AM270020|pid:none) Aspergillus niger contig An02c0250...    74   5e-12
(P50886) RecName: Full=60S ribosomal protein L22;  &(Q28IL6) Rec...    73   1e-11
AP007151_640(AP007151|pid:none) Aspergillus oryzae RIB40 genomic...    72   2e-11
protein update 2009. 7.31
PSORT 

 psg: 0.87  gvh: 0.46  alm: 0.41  top: 0.50  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.06  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 24.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VSF275 is cyt
5' end seq. ID VSF275F
5' end seq. 
>VSF275F.Seq
ATGCCAAACGTCCAAAAAAACCGTCGTAAAGAAATCTCATAAATTCGTCATTGATTGTAC
CGCTCCAGCTGGTAAAATCGTTGATGTTGCTGCTTTCGAAAAGTACTTACACGACAGAAT
CAAAGTTGACAACAAAGTTAGTAACCTCGGTTCAAATGTTGTTATCTCAAAAGATAAATC
AAAGATCATCATCAACACCACCATCCCATTCTCAAAGAGATACCTCAAATATTTAACAAA
GAAATTCTTAAAATTCAAACAAATCAGAGATTTCTTACGTGTTGTTGCCACCACCAAAAA
TACATACGAACTCAGATACTTCAACATTGGTGATTCTGAATCACAAGAATAAATTAATTT
TAATTTTTAATTTATTTTTTTTAAAAAAAAAA----------
Length of 5' end seq. 392
3' end seq. ID VSF275Z
3' end seq. 
>VSF275Z.Seq
----------ATGCCAAACGTCCAAAAAAACCGTCGTAAAGAAATCTCATAAATTCGTCA
TTGATTGTACCGCTCCAGCTGGTAAAATCGTTGATGTTGCTGCTTTCGAAAAGTACTTAC
ACGACAGAATCAAAGTTGACAACAAAGTTAGTAACCTCGGTTCAAATGTTGTTATCTCAA
AAGATAAATCAAAGATCATCATCAACACCACCATCCCATTCTCAAAGAGATACCTCAAAT
ATTTAACAAAGAAATTCTTAAAATTCAAACAAATCAGAGATTTCTTACGTGTTGTTGCCA
CCACCAAAAATACATACGAACTCAGATACTTCAACATTGGGATTCTGAATCACAAGAATA
AATTAATTTTAATTTTAA
Length of 3' end seq. 368
Connected seq. ID VSF275P
Connected seq. 
>VSF275P.Seq
ATGCCAAACGTCCAAAAAAACCGTCGTAAAGAAATCTCATAAATTCGTCATTGATTGTAC
CGCTCCAGCTGGTAAAATCGTTGATGTTGCTGCTTTCGAAAAGTACTTACACGACAGAAT
CAAAGTTGACAACAAAGTTAGTAACCTCGGTTCAAATGTTGTTATCTCAAAAGATAAATC
AAAGATCATCATCAACACCACCATCCCATTCTCAAAGAGATACCTCAAATATTTAACAAA
GAAATTCTTAAAATTCAAACAAATCAGAGATTTCTTACGTGTTGTTGCCACCACCAAAAA
TACATACGAACTCAGATACTTCAACATTGGTGATTCTGAATCACAAGAATAAATTAATTT
TAATTTTTAATTTATTTTTTTTAAAAAAAAAA----------ATGCCAAACGTCCAAAAA
AACCGTCGTAAAGAAATCTCATAAATTCGTCATTGATTGTACCGCTCCAGCTGGTAAAAT
CGTTGATGTTGCTGCTTTCGAAAAGTACTTACACGACAGAATCAAAGTTGACAACAAAGT
TAGTAACCTCGGTTCAAATGTTGTTATCTCAAAAGATAAATCAAAGATCATCATCAACAC
CACCATCCCATTCTCAAAGAGATACCTCAAATATTTAACAAAGAAATTCTTAAAATTCAA
ACAAATCAGAGATTTCTTACGTGTTGTTGCCACCACCAAAAATACATACGAACTCAGATA
CTTCAACATTGGGATTCTGAATCACAAGAATAAATTAATTTTAATTTTAA
Length of connected seq. 760
Full length Seq ID -
Full length Seq. -
Length of full length seq. -