>VSF265 (VSF265Q) /CSM/VS/VSF2-C/VSF265Q.Seq.d/
XXXXXXXXXXTTTACAGAAAAAGGTGCACGTAATGTTGTAAGACAAGTATGTGCTGGTGT
TGAATATCTCCATTCACAAGGTATTGCTCATAGAGATTTGAAACCAGAGAATTTATTATG
TAATGGTGAAGGTGAAGATATGACAATCAAAATTGCAGATTTTGGTCTTAGTAAAATATT
TGGAACTGGTNAAGCATTGNAAACAAGTTGTGGTACACCAGATTATGTTGCACCAGAAGT
TTTAACTGGTGGTTCATATGATAATGCAGTCGATATGTGGTCAATTGGTGTCATTACCTA
TATTTTATTATGTGGTTTCCCACCATTCCTATGCTTCATCTCACNACTTATTATTTGAAA
AAATTTTANCCGCTGATTATGATTTCCCAGAACCAGAATGGACTCATGTCTCTGAACATG
CCAAAGCTTTCATTAGAAACTTAATTGTCAAAGATCCAGATCAACGTCATACTGCCAAAC
AATGTCTTGATGACCTTTGGCTCTCTGGTAGTGATCAATCAATTGGNTCNGCTGCTGATC
NCCACTCTCANTNCG

---TEKGARNVVRQVCAGVEYLHSQGIAHRDLKPENLLCNGEGEDMTIKIADFGLSKIFG
TGXALXTSCGTPDYVAPEVLTGGSYDNAVDMWSIGVITYILLCGFPPFLCFISXLII*kn
fxr*l*fprtrmdscl*tcqsfh*klncqrsrstsycqtms**plalw**sinwxxc*sp
lsx

Frame A:
---lqkkvhvml*dkyvlvlnisihkvlliei*nqriyyvmvkvki*qsklqilvlvkyl
elvkhxkqvvvhqimlhqkf*lvvhmimqsicgqlvslpifyyvvshhsyasshxllfek
ilxadydfpepewthvsehakafirnlivkdpdqrhtakqclddlwlsgsdqsigsaadx
hsxx


Frame B:
---yrkrct*ccktsmcwc*ispftrycs*rfetrefim*w*r*rydnqncrfws**niw
nwxsixnklwytrlcctrsfnwwfi**csryvvnwchylyfimwfptipmlhlttyylkk
fxplimisqnqnglmslnmpklslet*lskiqinvilpnnvlmtfgslvvinqlxxllix
tlxx


Frame C:
---TEKGARNVVRQVCAGVEYLHSQGIAHRDLKPENLLCNGEGEDMTIKIADFGLSKIFG
TGXALXTSCGTPDYVAPEVLTGGSYDNAVDMWSIGVITYILLCGFPPFLCFISXLII*kn
fxr*l*fprtrmdscl*tcqsfh*klncqrsrstsycqtms**plalw**sinwxxc*sp
lsx

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSF265 (VSF265Q) /CSM/VS/VSF2-C/VSF265Q.Seq.d/                       1029   0.0
SSI101 (SSI101Q) /CSM/SS/SSI1-A/SSI101Q.Seq.d/                       1005   0.0
SFK820 (SFK820Q) /CSM/SF/SFK8-A/SFK820Q.Seq.d/                       1005   0.0
SFH150 (SFH150Q) /CSM/SF/SFH1-C/SFH150Q.Seq.d/                        999   0.0
SSC643 (SSC643Q) /CSM/SS/SSC6-B/SSC643Q.Seq.d/                        989   0.0
AFL780 (AFL780Q) /CSM/AF/AFL7-D/AFL780Q.Seq.d/                        989   0.0
CFB218 (CFB218Q) /CSM/CF/CFB2-A/CFB218Q.Seq.d/                        973   0.0
VFG501 (VFG501Q) /CSM/VF/VFG5-A/VFG501Q.Seq.d/                        969   0.0
VFJ379 (VFJ379Q) /CSM/VF/VFJ3-D/VFJ379Q.Seq.d/                        967   0.0
VFG381 (VFG381Q) /CSM/VF/VFG3-D/VFG381Q.Seq.d/                        967   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   618  0.0  2
M64176|M64176.1 D.discoideum myosin light chain kinase (MLCK) mRNA, complete cds.    80  3e-23  3
AZ667950|AZ667950.1 ENTFN88TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52  4e-15  3
AZ545332|AZ545332.1 ENTGA35TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52  3e-11  3
BH137166|BH137166.1 ENTNR83TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52  6e-07  2
AZ672839|AZ672839.1 ENTIX25TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52  8e-07  2
AZ930615|AZ930615.1 474.dhz56f04.s1 Saccharomyces unisporus NRRL Y-1556 Saccharomyces unisporus genomic clone 474.dhz56f04.s1, DNA sequence.    42  2e-05  3
BP190282|BP190282.1 Dugesia japonica cDNA, clone: 04589_HH, expressed in the head.    60  3e-05  1
AZ676962|AZ676962.1 ENTJV60TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52  2e-04  2
AC116977|AC116977.2 Dictyostelium discoideum chromosome 2 map 5515173-5817331 strain AX4, complete sequence.    56  2e-04  8

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q869W6) RecName: Full=Probable myosin light chain kinase DDB_G0...   219   5e-91
(Q86AD7) RecName: Full=Probable myosin light chain kinase DDB_G0...   150   7e-48
BC074183_1(BC074183|pid:none) Xenopus laevis MGC82022 protein, m...   134   2e-43
(Q8IU85) RecName: Full=Calcium/calmodulin-dependent protein kina...   135   6e-43
(Q8BW96) RecName: Full=Calcium/calmodulin-dependent protein kina...   135   6e-43
BC110966_1(BC110966|pid:none) Xenopus laevis calcium/calmodulin-...   137   6e-43
AK052401_1(AK052401|pid:none) Mus musculus 13 days embryo lung c...   135   6e-43
AY335686_1(AY335686|pid:none) Synthetic construct Homo sapiens C...   135   6e-43
BC041721_1(BC041721|pid:none) Xenopus laevis calcium/calmodulin-...   137   2e-42
AB076903_1(AB076903|pid:none) Ciona intestinalis Ci-CaM-K mRNA f...   132   4e-42

 psg: 0.72  gvh: 0.34  alm: 0.26  top: 0.73  tms: 0.07  mit: 0.32  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.05  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 1.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: cytoplasmic
	 20.0 %: nuclear
	 16.0 %: vesicles of secretory system
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  8.0 %: endoplasmic reticulum
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for VSF265 is cyt

>VSF265Z.Seq
----------TTTACAGAAAAAGGTGCACGTAATGTTGTAAGACAAGTATGTGCTGGTGT
TGAATATCTCCATTCACAAGGTATTGCTCATAGAGATTTGAAACCAGAGAATTTATTATG
TAATGGTGAAGGTGAAGATATGACAATCAAAATTGCAGATTTTGGTCTTAGTAAAATATT
TGGAACTGGTNAAGCATTGNAAACAAGTTGTGGTACACCAGATTATGTTGCACCAGAAGT
TTTAACTGGTGGTTCATATGATAATGCAGTCGATATGTGGTCAATTGGTGTCATTACCTA
TATTTTATTATGTGGTTTCCCACCATTCCTATGCTTCATCTCACNACTTATTATTTGAAA
AAATTTTANCCGCTGATTATGATTTCCCAGAACCAGAATGGACTCATGTCTCTGAACATG
CCAAAGCTTTCATTAGAAACTTAATTGTCAAAGATCCAGATCAACGTCATACTGCCAAAC
AATGTCTTGATGACCTTTGGCTCTCTGGTAGTGATCAATCAATTGGNTCNGCTGCTGATC
NCCACTCTCANTNCG