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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSF227
Library VS
(Link to library)
Clone ID VSF227
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U05551-1
Original site URL
Representative seq. ID VSF227P
(Link to Original site)
Representative DNA sequence 
>VSF227 (VSF227Q) /CSM/VS/VSF2-B/VSF227Q.Seq.d/
CCCACATACATATATATAAAATNATGGAAAATAGATTAACCACTAGCTCAATTGTCACTA
GACCATTAACCCCAAGATATCCAGTTATTATTAAAGATCCAACAAATAAGGATGTTATAT
CTAACTTTAGATTAGGTGATCATTTCTTAGCTTTAGGTGTTACAACCATCCTTACATCTG
GTTATTACTATTCAACTTATCTTCATAAACCATCAACTATTTTATGGGGTGCATTTATTT
TACCAACTATGTACCGTTTTAAGTGGTGAACGTGTTTCACAAAGATTAAAGGGTGTACAA
GAAAATCAAAAAGATTGTGCTAAATATGGTATTGAATTCACTCAAAATATAACTCCATAC
AATTTATAAATTACAATACAGAAAAAAAAAAATAGTAATAATAATAATATAAAAAAAAAA
XXXXXXXXXXCCACGNGTCCGCCCACATACATATATATAAAATCATGGAAAATAGATTAA
CCACTAGCTCAATTGTCACTAGACCATTAACCCCAAGATATCCAGTTATTATTAAAGATC
CAACAAATAAGGATGTTATATCTAACTTTAGATTAGGTGATCATTTCTTAGCTTTAGGTG
TTACAACCATCCTTACATCTGGTTATTACTATTCAACTTATCTTCATAAACCATCAACTA
TTTTATGGGGTGCATTTATTTTACCAACTATGNACGTTTTAAGTGGTGAACGTGTTTNAC
AAAGATTAAAGGGTGNNCAANAAAANCAAAAAGATTGNGCTAAATATGGTATTGAATTCA
C
sequence update 2001. 3.22
Translated Amino Acid sequence 
ptyiyikxwkid*plaqlsldh*pqdiqlllkiqqirmlyltld*viis*l*vlqpslhl
vitiqlifinhqlfygvhlfyqlctvlsgervsqrlkgvqenqkdcakygieftqnitpy
nl*ITIQKKKNSNNNNIKKK---

---HXSAHIHIYKIMENRLTTSSIVTRPLTPRYPVIIKDPTNKDVISNFRLGDHFLALGV
TTILTSGYYYSTYLHKPSTILWGAFILPTMXVLSGERVXQRLKGXQXXQKDXAKYGIEF
Translated Amino Acid sequence (All Frames) 
Frame A:
ptyiyikxwkid*plaqlsldh*pqdiqlllkiqqirmlyltld*viis*l*vlqpslhl
vitiqlifinhqlfygvhlfyqlctvlsgervsqrlkgvqenqkdcakygieftqnitpy
nl*ITIQKKKNSNNNNIKKK---

---prvrphtyi*nhgk*inh*lnch*tinpkissyy*rsnk*gcyi*l*ir*sflsfrc
ynhpyiwlllfnlss*tinyfmgciyftnyxrfkw*tcxtkikgxxxkxkrlx*iwy*ih

Frame B:
phtyi*nxgk*inh*lnch*tinpkissyy*rsnk*gcyi*l*ir*sflsfrcynhpyiw
lllfnlss*tinyfmgciyftnyvpf*vvnvfhkd*rvykkikkivlnmvlnslki*lht
iyklqyrkkkiviiii*kk---

---HXSAHIHIYKIMENRLTTSSIVTRPLTPRYPVIIKDPTNKDVISNFRLGDHFLALGV
TTILTSGYYYSTYLHKPSTILWGAFILPTMXVLSGERVXQRLKGXQXXQKDXAKYGIEF

Frame C:
hihiykxmenrlttssivtrpltprypviikdptnkdvisnfrlgdhflalgvttiltsg
yyystylhkpstilwgafilptmyrfkw*tcftkikgctrkskrlc*iwy*ihskynsiq
finyntekkk*****ykkk---

---txpptyiyikswkid*plaqlsldh*pqdiqlllkiqqirmlyltld*viis*l*vl
qpslhlvitiqlifinhqlfygvhlfyqlxtf*vvnvfxkd*rvxxkxkkixlnmvlns
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSF227 (VSF227Q) /CSM/VS/VSF2-B/VSF227Q.Seq.d/                        749   0.0
VSH883 (VSH883Q) /CSM/VS/VSH8-D/VSH883Q.Seq.d/                        733   0.0
VSH881 (VSH881Q) /CSM/VS/VSH8-D/VSH881Q.Seq.d/                        733   0.0
VSC447 (VSC447Q) /CSM/VS/VSC4-B/VSC447Q.Seq.d/                        733   0.0
SSJ780 (SSJ780Q) /CSM/SS/SSJ7-D/SSJ780Q.Seq.d/                        728   0.0
VSF341 (VSF341Q) /CSM/VS/VSF3-B/VSF341Q.Seq.d/                        523   e-147
SSJ477 (SSJ477Q) /CSM/SS/SSJ4-D/SSJ477Q.Seq.d/                        432   e-120
VSJ490 (VSJ490Q) /CSM/VS/VSJ4-D/VSJ490Q.Seq.d/                        129   7e-29
VSI754 (VSI754Q) /CSM/VS/VSI7-C/VSI754Q.Seq.d/                         36   0.77
VSG202 (VSG202Q) /CSM/VS/VSG2-A/VSG202Q.Seq.d/                         36   0.77
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AL929352|AL929352.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 2/4.    36  0.014  5
AF361475|AF361475.1 Dictyostelium discoideum double histidine kinase DhkD (dhkD) gene, complete cds.    34  0.015  4
AC124215|AC124215.13 Medicago truncatula clone mth2-32m6, WORKING DRAFT SEQUENCE, 5 ordered pieces.    50  0.026  3
AL049184|AL049184.5 Plasmodium falciparum DNA *** SEQUENCING IN PROGRESS *** from  MAL13P3.    44  0.096  5
L22219|L22219.1 Plasmodium falciparum transmission-blocking target antigen (s230) gene, 5' end.    38  0.15  3
AF269242|AF269242.1 Plasmodium falciparum transmission-blocking target antigen Pfs230 (s230) gene, complete cds.    38  0.15  3
I95887|I95887.1 Sequence 1 from patent US 5733772.                38  0.15  3
AR178096|AR178096.1 Sequence 1 from patent US 6316000.            38  0.15  3
L08135|L08135.1 Plasmodium falciparum transmission blocking target antigen (Ps230) mRNA, complete cds.    38  0.15  3
L04162|L04162.1 Plasmodium falciparum (clone sY) s230 mRNA, complete cds.    38  0.15  3
dna update 2003. 8.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY365059_1(AY365059|pid:none) Chlamydomonas reinhardtii NADH:ubi...    35   3.6
protein update 2009. 3.22
PSORT 

 psg: 0.45  gvh: 0.43  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.08
 nuc: 0.02  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 28.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VSF227 is nuc
5' end seq. ID VSF227F
5' end seq. 
>VSF227F.Seq
CCCACATACATATATATAAAATNATGGAAAATAGATTAACCACTAGCTCAATTGTCACTA
GACCATTAACCCCAAGATATCCAGTTATTATTAAAGATCCAACAAATAAGGATGTTATAT
CTAACTTTAGATTAGGTGATCATTTCTTAGCTTTAGGTGTTACAACCATCCTTACATCTG
GTTATTACTATTCAACTTATCTTCATAAACCATCAACTATTTTATGGGGTGCATTTATTT
TACCAACTATGTACCGTTTTAAGTGGTGAACGTGTTTCACAAAGATTAAAGGGTGTACAA
GAAAATCAAAAAGATTGTGCTAAATATGGTATTGAATTCACTCAAAATATAACTCCATAC
AATTTATAAATTACAATACAGAAAAAAAAAAATAGTAATAATAATAATATAAAAAAAAAA
----------
Length of 5' end seq. 420
3' end seq. ID VSF227Z
3' end seq. 
>VSF227Z.Seq
----------CCACGNGTCCGCCCACATACATATATATAAAATCATGGAAAATAGATTAA
CCACTAGCTCAATTGTCACTAGACCATTAACCCCAAGATATCCAGTTATTATTAAAGATC
CAACAAATAAGGATGTTATATCTAACTTTAGATTAGGTGATCATTTCTTAGCTTTAGGTG
TTACAACCATCCTTACATCTGGTTATTACTATTCAACTTATCTTCATAAACCATCAACTA
TTTTATGGGGTGCATTTATTTTACCAACTATGNACGTTTTAAGTGGTGAACGTGTTTNAC
AAAGATTAAAGGGTGNNCAANAAAANCAAAAAGATTGNGCTAAATATGGTATTGAATTCA
C
Length of 3' end seq. 351
Connected seq. ID VSF227P
Connected seq. 
>VSF227P.Seq
CCCACATACATATATATAAAATNATGGAAAATAGATTAACCACTAGCTCAATTGTCACTA
GACCATTAACCCCAAGATATCCAGTTATTATTAAAGATCCAACAAATAAGGATGTTATAT
CTAACTTTAGATTAGGTGATCATTTCTTAGCTTTAGGTGTTACAACCATCCTTACATCTG
GTTATTACTATTCAACTTATCTTCATAAACCATCAACTATTTTATGGGGTGCATTTATTT
TACCAACTATGTACCGTTTTAAGTGGTGAACGTGTTTCACAAAGATTAAAGGGTGTACAA
GAAAATCAAAAAGATTGTGCTAAATATGGTATTGAATTCACTCAAAATATAACTCCATAC
AATTTATAAATTACAATACAGAAAAAAAAAAATAGTAATAATAATAATATAAAAAAAAAA
----------CCACGNGTCCGCCCACATACATATATATAAAATCATGGAAAATAGATTAA
CCACTAGCTCAATTGTCACTAGACCATTAACCCCAAGATATCCAGTTATTATTAAAGATC
CAACAAATAAGGATGTTATATCTAACTTTAGATTAGGTGATCATTTCTTAGCTTTAGGTG
TTACAACCATCCTTACATCTGGTTATTACTATTCAACTTATCTTCATAAACCATCAACTA
TTTTATGGGGTGCATTTATTTTACCAACTATGNACGTTTTAAGTGGTGAACGTGTTTNAC
AAAGATTAAAGGGTGNNCAANAAAANCAAAAAGATTGNGCTAAATATGGTATTGAATTCA
C
Length of connected seq. 771
Full length Seq ID -
Full length Seq. -
Length of full length seq. -