>VSF221 (VSF221Q) /CSM/VS/VSF2-A/VSF221Q.Seq.d/
ATATGAAAATGTTAATATATTAAATGTAACTAGAACAAAAGTAGCAAGTAGTTATTGTAT
TAAAACAAAATCATCAGCAGTAAAAAGATTCAGTTTAAAAAAAGGTAATGGTAAAGTAAA
ATTTCACCCAAGAGCAAAACCAGATACAACTGCCTACGTTAAAAGCAAAATCTATACAAA
TACATTCAAAGCTTGTTTATTTTCAGGTCACCCAGTTAGAAAAACACAAATAAGAACTAT
TAGTAATCCATTATTAATTGAAACTGTTATTCCAAATTAAAAAAAAAAXXXXXXXXXXAT
ATGAAAATGTTAATATATTAAATGTAACTAGAACAAAAGTAGCAAGTAGTTATTGTATTA
AAACAAAATCATCAGCAGTAAAAAGATTCAGTTTAAAAAAAGGTAATGGTAAAGTAAAAT
TTCACCCAAGAGCAAAACCAGATACAACTGCCTACGTTAAAAGCAAAATCTATACAAATA
CATTCAAAGCTTGTTTATTTTCAGGTCACCCAGTTAGAAAAAC

mkmliy*m*leqk*qvvivlkqnhqq*kdsv*kkvmvk*nftqeqnqiqlptlkaksiqi
hsklvyfqvtqlekhk*ellvihy*LKLLFQIKKK---

---YENVNILNVTRTKVASSYCIKTKSSAVKRFSLKKGNGKVKFHPRAKPDTTAYVKSKI
YTNTFKACLFSGHPVRK

Frame A:
i*kc*yikcn*nkssk*lly*nkiisskkiqfkkr*w*skispksktrynclr*kqnlyk
yiqslfifrsps*kntnkny**siin*ncysklkkk---

---i*kc*yikcn*nkssk*lly*nkiisskkiqfkkr*w*skispksktrynclr*kqn
lykyiqslfifrsps*kn

Frame B:
yenvnilnvtrtkvassyciktkssavkrfslkkgngkvkfhprakpdttayvkskiytn
tfkaclfsghpvrktqirtisnpllietvipn*kk---

---YENVNILNVTRTKVASSYCIKTKSSAVKRFSLKKGNGKVKFHPRAKPDTTAYVKSKI
YTNTFKACLFSGHPVRK

Frame C:
mkmliy*m*leqk*qvvivlkqnhqq*kdsv*kkvmvk*nftqeqnqiqlptlkaksiqi
hsklvyfqvtqlekhk*ellvihy*LKLLFQIKKK---

---mkmliy*m*leqk*qvvivlkqnhqq*kdsv*kkvmvk*nftqeqnqiqlptlkaks
iqihsklvyfqvtqlek

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSF234 (VSF234Q) /CSM/VS/VSF2-B/VSF234Q.Seq.d/                        509   e-143
VSF221 (VSF221Q) /CSM/VS/VSF2-A/VSF221Q.Seq.d/                        509   e-143
VSF810 (VSF810Q) /CSM/VS/VSF8-A/VSF810Q.Seq.d/                        349   3e-95
VSG459 (VSG459Q) /CSM/VS/VSG4-C/VSG459Q.Seq.d/                        305   4e-82
AHC260 (AHC260Q) /CSM/AH/AHC2-C/AHC260Q.Seq.d/                         36   0.51
VSF703 (VSF703Q) /CSM/VS/VSF7-A/VSF703Q.Seq.d/                         34   2.0
VHG252 (VHG252Q) /CSM/VH/VHG2-C/VHG252Q.Seq.d/                         34   2.0
VFG740 (VFG740Q) /CSM/VF/VFG7-B/VFG740Q.Seq.d/                         34   2.0
VFA142 (VFA142Q) /CSM/VF/VFA1-B/VFA142Q.Seq.d/                         34   2.0
SSL308 (SSL308Q) /CSM/SS/SSL3-A/SSL308Q.Seq.d/                         34   2.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC022906|AC022906.2 Homo sapiens chromosome 3 clone RP11-659P16 map 3, LOW-PASS SEQUENCE SAMPLING.    40  0.010  2
AC100441|AC100441.1 Mus musculus clone RP23-137G2, LOW-PASS SEQUENCE SAMPLING.    38  0.047  2
AC005140|AC005140.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    40  0.084  7
AC127691|AC127691.2 Mus musculus chromosome UNK clone RP24-216M10, WORKING DRAFT SEQUENCE, 6 unordered pieces.    48  0.11  3
BX571705|BX571705.2 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone DKEY-261E22.    36  0.13  5
AE014851|AE014851.1 Plasmodium falciparum 3D7 chromosome 12, section 8 of 9 of the complete sequence.    40  0.28  8
AC100636|AC100636.1 Mus musculus clone RP23-161N3, LOW-PASS SEQUENCE SAMPLING.    34  0.60  4
AC073732|AC073732.1 Mus musculus clone RP23-248G16, WORKING DRAFT SEQUENCE, 27 unordered pieces.    38  0.67  4
AC118750|AC118750.3 Mus musculus clone RP24-198J23, WORKING DRAFT SEQUENCE, 7 unordered pieces.    38  1.1  5
AC110096|AC110096.5 Rattus norvegicus clone CH230-225C11, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces.    44  1.2  3

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3268448
Number of Hits to DB: 652,728,631

 psg: 0.60  gvh: 0.36  alm: 0.51  top: 0.53  tms: 0.00  mit: 0.37  mip: 0.00
 nuc: 0.12  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 32.0 %: mitochondrial
	 20.0 %: cytoplasmic
	  4.0 %: cytoskeletal

>> prediction for VSF221 is nuc

>VSF221F.Seq
ATATGAAAATGTTAATATATTAAATGTAACTAGAACAAAAGTAGCAAGTAGTTATTGTAT
TAAAACAAAATCATCAGCAGTAAAAAGATTCAGTTTAAAAAAAGGTAATGGTAAAGTAAA
ATTTCACCCAAGAGCAAAACCAGATACAACTGCCTACGTTAAAAGCAAAATCTATACAAA
TACATTCAAAGCTTGTTTATTTTCAGGTCACCCAGTTAGAAAAACACAAATAAGAACTAT
TAGTAATCCATTATTAATTGAAACTGTTATTCCAAATTAAAAAAAAAA----------

>VSF221Z.Seq
----------ATATGAAAATGTTAATATATTAAATGTAACTAGAACAAAAGTAGCAAGTA
GTTATTGTATTAAAACAAAATCATCAGCAGTAAAAAGATTCAGTTTAAAAAAAGGTAATG
GTAAAGTAAAATTTCACCCAAGAGCAAAACCAGATACAACTGCCTACGTTAAAAGCAAAA
TCTATACAAATACATTCAAAGCTTGTTTATTTTCAGGTCACCCAGTTAGAAAAAC

>VSF221P.Seq
ATATGAAAATGTTAATATATTAAATGTAACTAGAACAAAAGTAGCAAGTAGTTATTGTAT
TAAAACAAAATCATCAGCAGTAAAAAGATTCAGTTTAAAAAAAGGTAATGGTAAAGTAAA
ATTTCACCCAAGAGCAAAACCAGATACAACTGCCTACGTTAAAAGCAAAATCTATACAAA
TACATTCAAAGCTTGTTTATTTTCAGGTCACCCAGTTAGAAAAACACAAATAAGAACTAT
TAGTAATCCATTATTAATTGAAACTGTTATTCCAAATTAAAAAAAAAA----------AT
ATGAAAATGTTAATATATTAAATGTAACTAGAACAAAAGTAGCAAGTAGTTATTGTATTA
AAACAAAATCATCAGCAGTAAAAAGATTCAGTTTAAAAAAAGGTAATGGTAAAGTAAAAT
TTCACCCAAGAGCAAAACCAGATACAACTGCCTACGTTAAAAGCAAAATCTATACAAATA
CATTCAAAGCTTGTTTATTTTCAGGTCACCCAGTTAGAAAAAC