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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSF220
Library VS
(Link to library)
Clone ID VSF220
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16097-1
Original site URL
Representative seq. ID VSF220P
(Link to Original site)
Representative DNA sequence 
>VSF220 (VSF220Q) /CSM/VS/VSF2-A/VSF220Q.Seq.d/
CGCGTCCGATTNGAAGCAGATGCCGCCAATTTAGTTAAAAAGATTGTTTCAGCTGTTGGA
TACCTTCATGGGTTAAATATCGTCCACAGAGATTTAAAACCAGAAAATTTACTTTTGAAA
TCTAAAGAAAACCATTTGGAAGTTGCTATTGCTGATTTTGGTCTTAGTAAAATTATTGGC
CAAACTCTTGTAATGCAAACTGCATGTGGTACACCAAGTTATGTTGCACCAGAGGTATTG
AATGCAACTGGTTATGATAAGGAAGTTGATATGTGGAGTATTGGTGTCATCACTTATATT
TTATTATGTGGTTTCCCACCATTCTACGGTGATACAGTCCCAGAAATCTTTGAACAAATT
ATGGAAGCCAACTATGAATTCCCAGAAGAATATTGGGGTGGTATCTCTAAAGAAGCCAAA
GACTTTATTGGAAAATTATTAGTTGTTGATGTTAGCAAAAGATTAAATGCTACCAATGCT
CTCAATCATCCTTGGTTAAAGTCCAATAACTCAAATAACACCATTGATACTGTTAAAATG
AAAGAATACATTGTTGAAAGACAAAAAACTCAAACAXXXXXXXXXXATTCAGAAGCAGAT
GCCGCCAATTTAGTTAAAAAGATTGTTTCAGCTGTTGGATACCTTCATGGGTTAAATATC
GTCCACAGAGATTTAAAACCAGAAAATTTACTTTTGAAATCTAAAGAAAACCATTTGGAA
GTTGCTATTGCTGATTTTGGTCTTAGTAAAATTATTGGCCAAACTCTTGTAATGCAAACT
GCATGTGGTACACCAAGTTATGTTGCACCAGAGGTATTGAATGCAACTGGTTATGATAAG
GAAGTTGATATGTGGAGTATTGGTGTCATCACTTATATTTTATTATGTGGTTTCCCACCA
TTCTACGGTGATACAGTCCCAGAAATCTTTGAACAAATTATGGAAGCCAACTATGAATTC
CCAGAAGAATATTGGGGTGGTATCTCTAAAGAAGCCAAAGACNAAATTGGAAAATNATNA
G
sequence update 2001. 3.22
Translated Amino Acid sequence 
RVRXEADAANLVKKIVSAVGYLHGLNIVHRDLKPENLLLKSKENHLEVAIADFGLSKIIG
QTLVMQTACGTPSYVAPEVLNATGYDKEVDMWSIGVITYILLCGFPPFYGDTVPEIFEQI
MEANYEFPEEYWGGISKEAKDFIGKLLVVDVSKRLNATNALNHPWLKSNNSNNTIDTVKM
KEYIVERQKTQT---

---SEADAANLVKKIVSAVGYLHGLNIVHRDLKPENLLLKSKENHLEVAIADFGLSKIIG
QTLVMQTACGTPSYVAPEVLNATGYDKEVDMWSIGVITYILLCGFPPFYGDTVPEIFEQI
MEANYEFPEEYWGGISKEAKDXIGKXX
Translated Amino Acid sequence (All Frames) 
Frame A:
RVRXEADAANLVKKIVSAVGYLHGLNIVHRDLKPENLLLKSKENHLEVAIADFGLSKIIG
QTLVMQTACGTPSYVAPEVLNATGYDKEVDMWSIGVITYILLCGFPPFYGDTVPEIFEQI
MEANYEFPEEYWGGISKEAKDFIGKLLVVDVSKRLNATNALNHPWLKSNNSNNTIDTVKM
KEYIVERQKTQT---

---iqkqmppi*lkrlfqlldtfmg*isstei*nqkiyf*nlkktiwkllllilvlvkll
akll*cklhvvhqvmlhqry*mqlvmirklicgvlvsslifyyvvshhstviqsqkslnk
lwkptmnsqknigvvslkkpktklenxx

Frame B:
asdxkqmppi*lkrlfqlldtfmg*isstei*nqkiyf*nlkktiwkllllilvlvklla
kll*cklhvvhqvmlhqry*mqlvmirklicgvlvsslifyyvvshhstviqsqkslnkl
wkptmnsqknigvvslkkpktlleny*llmlakd*mlpmlsiilg*spitqitplillk*
kntllkdkklk---

---frsrcrqfs*kdcfscwipswvkyrpqrfktrkftfei*rkpfgscyc*fws**nyw
pnscnancmwytklcctrgiecnwl**gs*yveywchhlyfimwfptilr*ysprnl*tn
ygsql*iprrilgwyl*rsqrxnwkxx

Frame C:
rpixsrcrqfs*kdcfscwipswvkyrpqrfktrkftfei*rkpfgscyc*fws**nywp
nscnancmwytklcctrgiecnwl**gs*yveywchhlyfimwfptilr*ysprnl*tny
gsql*iprrilgwyl*rsqrlywkiisc*c*qkikcyqcsqsslvkvq*lk*hh*yc*ne
rihc*ktknsn---

---SEADAANLVKKIVSAVGYLHGLNIVHRDLKPENLLLKSKENHLEVAIADFGLSKIIG
QTLVMQTACGTPSYVAPEVLNATGYDKEVDMWSIGVITYILLCGFPPFYGDTVPEIFEQI
MEANYEFPEEYWGGISKEAKDXIGKXX
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSF220 (VSF220Q) /CSM/VS/VSF2-A/VSF220Q.Seq.d/                       1941   0.0
VSE849 (VSE849Q) /CSM/VS/VSE8-C/VSE849Q.Seq.d/                       1118   0.0
SSG783 (SSG783Q) /CSM/SS/SSG7-D/SSG783Q.Seq.d/                       1118   0.0
SLE324 (SLE324Q) /CSM/SL/SLE3-A/SLE324Q.Seq.d/                       1118   0.0
SSJ247 (SSJ247Q) /CSM/SS/SSJ2-B/SSJ247Q.Seq.d/                       1066   0.0
SSM378 (SSM378Q) /CSM/SS/SSM3-D/SSM378Q.Seq.d/                       1057   0.0
VSE601 (VSE601Q) /CSM/VS/VSE6-A/VSE601Q.Seq.d/                       1053   0.0
SLI519 (SLI519Q) /CSM/SL/SLI5-A/SLI519Q.Seq.d/                        930   0.0
AFL629 (AFL629Q) /CSM/AF/AFL6-B/AFL629Q.Seq.d/                        839   0.0
VSE625 (VSE625Q) /CSM/VS/VSE6-B/VSE625Q.Seq.d/                        728   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

M64176|M64176.1 D.discoideum myosin light chain kinase (MLCK) mRNA, complete cds.  1118  0.0  1
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    84  1e-16  10
AZ667950|AZ667950.1 ENTFN88TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    54  1e-14  5
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    62  1e-09  8
BH137166|BH137166.1 ENTNR83TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    48  2e-08  3
BG809925|BG809925.1 mgct001xp07f Magnaporthe grisea Appressorium Stage cDNA Magnaporthe grisea cDNA clone mgct001xp07f 5', mRNA sequence.    36  1e-06  4
AZ672839|AZ672839.1 ENTIX25TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    48  2e-06  3
AZ545332|AZ545332.1 ENTGA35TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    44  4e-04  3
CB670932|CB670932.1 OSJNEe04E06.f OSJNEe Oryza sativa (japonica cultivar-group) cDNA clone OSJNEe04E06 5', mRNA sequence.    44  5e-04  2
AU293314|AU293314.1 Zinnia elegans cDNA, clone:Z8017, primer:M13 forward, sequence 1.    38  5e-04  4
dna update 2003. 8.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P25323) RecName: Full=Myosin light chain kinase A;          Sho...   382   e-104
A40811(A40811;A37125)myosin-light-chain kinase (EC 2.7.1.117) A ...   373   e-102
(Q54CY9) RecName: Full=Probable myosin light chain kinase DDB_G0...   208   2e-52
AB118103_1(AB118103|pid:none) Entamoeba histolytica gene for hyp...   206   8e-52
(Q86AD7) RecName: Full=Probable myosin light chain kinase DDB_G0...   200   8e-50
BC041721_1(BC041721|pid:none) Xenopus laevis calcium/calmodulin-...   194   3e-48
BC110966_1(BC110966|pid:none) Xenopus laevis calcium/calmodulin-...   193   7e-48
BC074183_1(BC074183|pid:none) Xenopus laevis MGC82022 protein, m...   192   1e-47
(Q8IU85) RecName: Full=Calcium/calmodulin-dependent protein kina...   191   4e-47
AY335686_1(AY335686|pid:none) Synthetic construct Homo sapiens C...   191   4e-47
protein update 2009. 7.31
PSORT 

 psg: 0.64  gvh: 0.47  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 24.0 %: nuclear
	 20.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for VSF220 is cyt
5' end seq. ID VSF220F
5' end seq. 
>VSF220F.Seq
CGCGTCCGATTNGAAGCAGATGCCGCCAATTTAGTTAAAAAGATTGTTTCAGCTGTTGGA
TACCTTCATGGGTTAAATATCGTCCACAGAGATTTAAAACCAGAAAATTTACTTTTGAAA
TCTAAAGAAAACCATTTGGAAGTTGCTATTGCTGATTTTGGTCTTAGTAAAATTATTGGC
CAAACTCTTGTAATGCAAACTGCATGTGGTACACCAAGTTATGTTGCACCAGAGGTATTG
AATGCAACTGGTTATGATAAGGAAGTTGATATGTGGAGTATTGGTGTCATCACTTATATT
TTATTATGTGGTTTCCCACCATTCTACGGTGATACAGTCCCAGAAATCTTTGAACAAATT
ATGGAAGCCAACTATGAATTCCCAGAAGAATATTGGGGTGGTATCTCTAAAGAAGCCAAA
GACTTTATTGGAAAATTATTAGTTGTTGATGTTAGCAAAAGATTAAATGCTACCAATGCT
CTCAATCATCCTTGGTTAAAGTCCAATAACTCAAATAACACCATTGATACTGTTAAAATG
AAAGAATACATTGTTGAAAGACAAAAAACTCAAACA----------
Length of 5' end seq. 576
3' end seq. ID VSF220Z
3' end seq. 
>VSF220Z.Seq
----------ATTCAGAAGCAGATGCCGCCAATTTAGTTAAAAAGATTGTTTCAGCTGTT
GGATACCTTCATGGGTTAAATATCGTCCACAGAGATTTAAAACCAGAAAATTTACTTTTG
AAATCTAAAGAAAACCATTTGGAAGTTGCTATTGCTGATTTTGGTCTTAGTAAAATTATT
GGCCAAACTCTTGTAATGCAAACTGCATGTGGTACACCAAGTTATGTTGCACCAGAGGTA
TTGAATGCAACTGGTTATGATAAGGAAGTTGATATGTGGAGTATTGGTGTCATCACTTAT
ATTTTATTATGTGGTTTCCCACCATTCTACGGTGATACAGTCCCAGAAATCTTTGAACAA
ATTATGGAAGCCAACTATGAATTCCCAGAAGAATATTGGGGTGGTATCTCTAAAGAAGCC
AAAGACNAAATTGGAAAATNATNAG
Length of 3' end seq. 435
Connected seq. ID VSF220P
Connected seq. 
>VSF220P.Seq
CGCGTCCGATTNGAAGCAGATGCCGCCAATTTAGTTAAAAAGATTGTTTCAGCTGTTGGA
TACCTTCATGGGTTAAATATCGTCCACAGAGATTTAAAACCAGAAAATTTACTTTTGAAA
TCTAAAGAAAACCATTTGGAAGTTGCTATTGCTGATTTTGGTCTTAGTAAAATTATTGGC
CAAACTCTTGTAATGCAAACTGCATGTGGTACACCAAGTTATGTTGCACCAGAGGTATTG
AATGCAACTGGTTATGATAAGGAAGTTGATATGTGGAGTATTGGTGTCATCACTTATATT
TTATTATGTGGTTTCCCACCATTCTACGGTGATACAGTCCCAGAAATCTTTGAACAAATT
ATGGAAGCCAACTATGAATTCCCAGAAGAATATTGGGGTGGTATCTCTAAAGAAGCCAAA
GACTTTATTGGAAAATTATTAGTTGTTGATGTTAGCAAAAGATTAAATGCTACCAATGCT
CTCAATCATCCTTGGTTAAAGTCCAATAACTCAAATAACACCATTGATACTGTTAAAATG
AAAGAATACATTGTTGAAAGACAAAAAACTCAAACA----------ATTCAGAAGCAGAT
GCCGCCAATTTAGTTAAAAAGATTGTTTCAGCTGTTGGATACCTTCATGGGTTAAATATC
GTCCACAGAGATTTAAAACCAGAAAATTTACTTTTGAAATCTAAAGAAAACCATTTGGAA
GTTGCTATTGCTGATTTTGGTCTTAGTAAAATTATTGGCCAAACTCTTGTAATGCAAACT
GCATGTGGTACACCAAGTTATGTTGCACCAGAGGTATTGAATGCAACTGGTTATGATAAG
GAAGTTGATATGTGGAGTATTGGTGTCATCACTTATATTTTATTATGTGGTTTCCCACCA
TTCTACGGTGATACAGTCCCAGAAATCTTTGAACAAATTATGGAAGCCAACTATGAATTC
CCAGAAGAATATTGGGGTGGTATCTCTAAAGAAGCCAAAGACNAAATTGGAAAATNATNA
G
Length of connected seq. 1011
Full length Seq ID -
Full length Seq. -
Length of full length seq. -