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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSF218
Library VS
(Link to library)
Clone ID VSF218
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VSF218P
(Link to Original site)
Representative DNA sequence 
>VSF218 (VSF218Q) /CSM/VS/VSF2-A/VSF218Q.Seq.d/
GCTGAATTCGTCTTTGGTTGGTATCTCACAATTAATTTCCAAGTTAGTCATGTCGCTGAA
GATCTCAAATTCTTTGCTACCCCTGAAAGACCAGATGAACCATCTCAAATCAATGAAGAT
TGGGCAATCCTTCAACTTAAAACTACTCAAGATTATGGTCATGGTTCACTCCTTTGTACC
TTTTTTAGTGGTTCTTTAAATCATCAAGTTGTTCATCATTTATTCCCATCAATTGCTCAA
GATTTCTACCCACAACTTGTACCAATTGTAAAAGAAGTTTGTAAAGAACATAACATTACT
TACCACATTAAACCAAACTTCACTGAAGCTATTATGTCACACATTAATTACCTTTACAAA
ATGGGTAATGATCCAGATTATGTTAAAAAACCATTAGCCTCAAAAGATGATTAAATGAAA
TAACTTAAAAACCAATTATTTACTTTTGACAAACAGTAATATTAATAAATACAAAAAAAA
AAXXXXXXXXXXGNTGAATTCGTCTTTGGTTGGTATCTCACAATTAATTTCCAAGTTAGT
CATGTCGCTGAAGATCTCAAATTCTTTGCTACCCCTGAAAGACCAGATGAACCATCTCAA
ATCAATGAAGATTGGGCAATCCTTCAACTTAAAACTACTCAAGATTATGGTCATGGTTCA
CTCCTTTGTACCTTTTTTAGTGGTTCTTTAAATCATCAAGTTGTTCATCATTTATTCCCA
TCAATTGCTCAAGATTTCTACCCACAACTTGTACCAATTGTAAAAGAAGTTTGTAAAGAA
CATAACATTACTTACCACATTAAACCAAACTTCACTGNAGCTATTATGTCACACATTAAT
TACCTTTACAAAATGGGTAATGATCCAGATTATGTTAAAAAACCATTAGCCTCAAAAGAT
GAAAAAAAGGAATAAC
sequence update 2001. 3.22
Translated Amino Acid sequence 
lnsslvgisqlisklvmslkisnsllplkdqmnhlksmkigqsfnlkllkimvmvhsfvp
flvvl*iiklfiiyshqllkisthnlyql*kkfvknitllttlnqtslkllchtlitftk
wvmiqimlknh*pqkmik*NNLKTNYLLLTNSNINKYKKK---

---XEFVFGWYLTINFQVSHVAEDLKFFATPERPDEPSQINEDWAILQLKTTQDYGHGSL
LCTFFSGSLNHQVVHHLFPSIAQDFYPQLVPIVKEVCKEHNITYHIKPNFTXAIMSHINY
LYKMGNDPDYVKKPLASKDEKKE*
Translated Amino Acid sequence (All Frames) 
Frame A:
aefvfgwyltinfqvshvaedlkffatperpdepsqinedwailqlkttqdyghgsllct
ffsgslnhqvvhhlfpsiaqdfypqlvpivkevckehnityhikpnfteaimshinylyk
mgndpdyvkkplaskdd*mk*lknqlftfdkq*y**iqkk---

---XEFVFGWYLTINFQVSHVAEDLKFFATPERPDEPSQINEDWAILQLKTTQDYGHGSL
LCTFFSGSLNHQVVHHLFPSIAQDFYPQLVPIVKEVCKEHNITYHIKPNFTXAIMSHINY
LYKMGNDPDYVKKPLASKDEKKE*

Frame B:
lnsslvgisqlisklvmslkisnsllplkdqmnhlksmkigqsfnlkllkimvmvhsfvp
flvvl*iiklfiiyshqllkisthnlyql*kkfvknitllttlnqtslkllchtlitftk
wvmiqimlknh*pqkmik*NNLKTNYLLLTNSNINKYKKK---

---xnsslvgisqlisklvmslkisnsllplkdqmnhlksmkigqsfnlkllkimvmvhs
fvpflvvl*iiklfiiyshqllkisthnlyql*kkfvknitllttlnqtslxllchtlit
ftkwvmiqimlknh*pqkmkkrnn

Frame C:
*irlwlvshn*fps*scr*rsqilcyp*ktr*tisnq*rlgnpst*nysrlwswftplyl
f*wffkssscssfipincsrflpttctnckrsl*rt*hylph*tklh*syyvth*lplqn
g**srlc*ktislkr*lneit*kpiiyf*qtvilintkkk---

---*irlwlvshn*fps*scr*rsqilcyp*ktr*tisnq*rlgnpst*nysrlwswftp
lylf*wffkssscssfipincsrflpttctnckrsl*rt*hylph*tklhxsyyvth*lp
lqng**srlc*ktislkr*kkgi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSF218 (VSF218Q) /CSM/VS/VSF2-A/VSF218Q.Seq.d/                        936   0.0
VSC833 (VSC833Q) /CSM/VS/VSC8-B/VSC833Q.Seq.d/                        936   0.0
SSB813 (SSB813Q) /CSM/SS/SSB8-A/SSB813Q.Seq.d/                        936   0.0
SLG121 (SLG121Q) /CSM/SL/SLG1-A/SLG121Q.Seq.d/                        936   0.0
SLE621 (SLE621Q) /CSM/SL/SLE6-A/SLE621Q.Seq.d/                        936   0.0
SLC603 (SLC603Q) /CSM/SL/SLC6-A/SLC603Q.Seq.d/                        936   0.0
SLA259 (SLA259Q) /CSM/SL/SLA2-C/SLA259Q.Seq.d/                        936   0.0
SLA256 (SLA256Q) /CSM/SL/SLA2-C/SLA256Q.Seq.d/                        936   0.0
VSF803 (VSF803Q) /CSM/VS/VSF8-A/VSF803Q.Seq.d/                        884   0.0
VFN880 (VFN880Q) /CSM/VF/VFN8-D/VFN880Q.Seq.d/                        835   0.0
own update 2001.12. 5
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BD092935|BD092935.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids in plants.   936  0.0  1
BD082645|BD082645.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids.   936  0.0  1
BD082630|BD082630.1 Methods and compositions for synthesis of long chain poly-unsaturated fatty acids.   936  0.0  1
AB029311|AB029311.1 Dictyostelium discoideum Dd des5 gene for fatty acid desaturase, complete cds.   496  0.0  2
AB022097|AB022097.1 Dictyostelium discoideum mRNA for delta 5 fatty acid desaturase, complete cds.    90  3e-44  5
AC107492|AC107492.11 Rattus norvegicus clone CH230-86P18, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    40  0.009  4
AC136561|AC136561.3 Rattus norvegicus clone CH230-90E22, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    40  0.009  4
BX465208|BX465208.4 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    40  0.21  6
BZ049099|BZ049099.1 jnr21b05.b1 B.oleracea001 Brassica oleracea genomic, DNA sequence.    38  0.27  2
BZ516756|BZ516756.1 BOMRH14TR BO_2_3_KB Brassica oleracea genomic clone BOMRH14, DNA sequence.    38  0.31  2
dna update 2003. 8.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9Y1W0) RecName: Full=Delta(5) fatty acid desaturase A;        ...   292   1e-77
(O96099) RecName: Full=Delta(5) fatty acid desaturase B;        ...   209   1e-52
(Q1ZXQ5) RecName: Full=Probable Delta(5) fatty acid desaturase C...   164   3e-39
AF419297_1(AF419297|pid:none) Pythium irregulare delta-5 fatty a...   129   2e-28
CP000592_278(CP000592|pid:none) Ostreococcus lucimarinus CCE9901...   118   3e-25
AB188307_1(AB188307|pid:none) Mortierella alpina gene for delta5...   115   2e-24
(O74212) RecName: Full=Delta(5) fatty acid desaturase;          ...   115   3e-24
CS020137_1(CS020137|pid:none) Sequence 91 from Patent WO20050123...   109   1e-22
CP001574_328(CP001574|pid:none) Micromonas sp. RCC299 chromosome...   107   6e-22
EF678404_1(EF678404|pid:none) Picea sitchensis clone WS0291_A04 ...   105   3e-21
protein update 2009. 7.31
PSORT 

 psg: 0.79  gvh: 0.30  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 32.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for VSF218 is cyt
5' end seq. ID VSF218F
5' end seq. 
>VSF218F.Seq
GCTGAATTCGTCTTTGGTTGGTATCTCACAATTAATTTCCAAGTTAGTCATGTCGCTGAA
GATCTCAAATTCTTTGCTACCCCTGAAAGACCAGATGAACCATCTCAAATCAATGAAGAT
TGGGCAATCCTTCAACTTAAAACTACTCAAGATTATGGTCATGGTTCACTCCTTTGTACC
TTTTTTAGTGGTTCTTTAAATCATCAAGTTGTTCATCATTTATTCCCATCAATTGCTCAA
GATTTCTACCCACAACTTGTACCAATTGTAAAAGAAGTTTGTAAAGAACATAACATTACT
TACCACATTAAACCAAACTTCACTGAAGCTATTATGTCACACATTAATTACCTTTACAAA
ATGGGTAATGATCCAGATTATGTTAAAAAACCATTAGCCTCAAAAGATGATTAAATGAAA
TAACTTAAAAACCAATTATTTACTTTTGACAAACAGTAATATTAATAAATACAAAAAAAA
AA----------
Length of 5' end seq. 482
3' end seq. ID VSF218Z
3' end seq. 
>VSF218Z.Seq
----------GNTGAATTCGTCTTTGGTTGGTATCTCACAATTAATTTCCAAGTTAGTCA
TGTCGCTGAAGATCTCAAATTCTTTGCTACCCCTGAAAGACCAGATGAACCATCTCAAAT
CAATGAAGATTGGGCAATCCTTCAACTTAAAACTACTCAAGATTATGGTCATGGTTCACT
CCTTTGTACCTTTTTTAGTGGTTCTTTAAATCATCAAGTTGTTCATCATTTATTCCCATC
AATTGCTCAAGATTTCTACCCACAACTTGTACCAATTGTAAAAGAAGTTTGTAAAGAACA
TAACATTACTTACCACATTAAACCAAACTTCACTGNAGCTATTATGTCACACATTAATTA
CCTTTACAAAATGGGTAATGATCCAGATTATGTTAAAAAACCATTAGCCTCAAAAGATGA
AAAAAAGGAATAAC
Length of 3' end seq. 424
Connected seq. ID VSF218P
Connected seq. 
>VSF218P.Seq
GCTGAATTCGTCTTTGGTTGGTATCTCACAATTAATTTCCAAGTTAGTCATGTCGCTGAA
GATCTCAAATTCTTTGCTACCCCTGAAAGACCAGATGAACCATCTCAAATCAATGAAGAT
TGGGCAATCCTTCAACTTAAAACTACTCAAGATTATGGTCATGGTTCACTCCTTTGTACC
TTTTTTAGTGGTTCTTTAAATCATCAAGTTGTTCATCATTTATTCCCATCAATTGCTCAA
GATTTCTACCCACAACTTGTACCAATTGTAAAAGAAGTTTGTAAAGAACATAACATTACT
TACCACATTAAACCAAACTTCACTGAAGCTATTATGTCACACATTAATTACCTTTACAAA
ATGGGTAATGATCCAGATTATGTTAAAAAACCATTAGCCTCAAAAGATGATTAAATGAAA
TAACTTAAAAACCAATTATTTACTTTTGACAAACAGTAATATTAATAAATACAAAAAAAA
AA----------GNTGAATTCGTCTTTGGTTGGTATCTCACAATTAATTTCCAAGTTAGT
CATGTCGCTGAAGATCTCAAATTCTTTGCTACCCCTGAAAGACCAGATGAACCATCTCAA
ATCAATGAAGATTGGGCAATCCTTCAACTTAAAACTACTCAAGATTATGGTCATGGTTCA
CTCCTTTGTACCTTTTTTAGTGGTTCTTTAAATCATCAAGTTGTTCATCATTTATTCCCA
TCAATTGCTCAAGATTTCTACCCACAACTTGTACCAATTGTAAAAGAAGTTTGTAAAGAA
CATAACATTACTTACCACATTAAACCAAACTTCACTGNAGCTATTATGTCACACATTAAT
TACCTTTACAAAATGGGTAATGATCCAGATTATGTTAAAAAACCATTAGCCTCAAAAGAT
GAAAAAAAGGAATAAC
Length of connected seq. 906
Full length Seq ID -
Full length Seq. -
Length of full length seq. -