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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSF215
Library VS
(Link to library)
Clone ID VSF215
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U03055-1
Original site URL
Representative seq. ID VSF215F
(Link to Original site)
Representative DNA sequence 
>VSF215 (VSF215Q) /CSM/VS/VSF2-A/VSF215Q.Seq.d/
GAAGAAGAAATCCGTGAAGCATTCAAAGTTTTCGATAAAGACGGTAACGGTTACATCTCA
GCTGCTGAACTTAGACACGTTATGACCAGTCTTGGTGAAAAACTTACCAACGAAGAAGTT
GATGAAATGATTAGAGAAGCTGATTTGGATGGCGATGGTCAAGTTAACTATGACGAATTT
GTTAAAATGATGATTGTTAGAAATTAAGAAAATTTAATATTAAAAAAAAACAAAAACCTT
GAAAAAAAAAAAAAAAAACCAAATTTTTTTTTTTTAANTAAAAAACCCCNTTTTTTTTAN
NGNAAAAAAAAAXXXXXXXXXX
sequence update 2001. 3.22
Translated Amino Acid sequence 
EEEIREAFKVFDKDGNGYISAAELRHVMTSLGEKLTNEEVDEMIREADLDGDGQVNYDEF
VKMMIVRN*enlilkknknlekkkkkpnffflxkkpxffxxkk---
Translated Amino Acid sequence (All Frames) 
Frame A:
EEEIREAFKVFDKDGNGYISAAELRHVMTSLGEKLTNEEVDEMIREADLDGDGQVNYDEF
VKMMIVRN*enlilkknknlekkkkkpnffflxkkpxffxxkk---


Frame B:
kkksvkhskfsiktvtvtsqllnldtl*pvlvknlptkklmk*lekliwmamvkltmtnl
lk**lleikki*y*kktktlkkkkknqifff*xknpxffxxkk---


Frame C:
rrnp*siqsfr*rr*rlhlsc*t*trydqsw*ktyqrrs**nd*rs*fgwrwss*l*ric
*nddc*klrkfnikkkqkp*kkkkktkffffx*ktpfflxxkk---
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSF215 (VSF215Q) /CSM/VS/VSF2-A/VSF215Q.Seq.d/                        438   e-122
VSE268 (VSE268Q) /CSM/VS/VSE2-C/VSE268Q.Seq.d/                        438   e-122
VSD126 (VSD126Q) /CSM/VS/VSD1-B/VSD126Q.Seq.d/                        438   e-122
VSD124 (VSD124Q) /CSM/VS/VSD1-A/VSD124Q.Seq.d/                        438   e-122
SSM156 (SSM156Q) /CSM/SS/SSM1-C/SSM156Q.Seq.d/                        438   e-122
SSM151 (SSM151Q) /CSM/SS/SSM1-C/SSM151Q.Seq.d/                        438   e-122
SSL774 (SSL774Q) /CSM/SS/SSL7-D/SSL774Q.Seq.d/                        438   e-122
SSL632 (SSL632Q) /CSM/SS/SSL6-B/SSL632Q.Seq.d/                        438   e-122
SSL130 (SSL130Q) /CSM/SS/SSL1-B/SSL130Q.Seq.d/                        438   e-122
SSK610 (SSK610Q) /CSM/SS/SSK6-A/SSK610Q.Seq.d/                        438   e-122
own update 2001.11.13
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

M64089|M64089.1 Dictyostelium discoideum calmodulin (calA) gene, complete cds.   438  e-119  1
M13009|M13009.1 D.discoideum calmodulin mRNA, partial cds.       438  e-119  1
BW049016|BW049016.1 Ciona intestinalis cDNA, clone:cibd066e04, 5' end, single read.   139  2e-29  1
BW056144|BW056144.1 Ciona intestinalis cDNA, clone:cibd085i24, 5' end, single read.   137  9e-29  1
AV896823|AV896823.1 Ciona intestinalis cDNA, clone:rciad46i07, 3' end, single read.   137  9e-29  1
AV905771|AV905771.1 Ciona intestinalis cDNA, clone:rciad63m01, 3' end, single read.   137  9e-29  1
BW040349|BW040349.1 Ciona intestinalis cDNA, clone:cibd043e04, 5' end, single read.   137  9e-29  1
BW318188|BW318188.1 Ciona intestinalis cDNA, clone:ciht037o16, 5' end, single read.   137  9e-29  1
BW044070|BW044070.1 Ciona intestinalis cDNA, clone:cibd054i07, 5' end, single read.   137  9e-29  1
BW026437|BW026437.1 Ciona intestinalis cDNA, clone:rcibd083k06, 3' end, single read.   137  9e-29  1
dna update 2003. 8.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

M13009_1(M13009|pid:none) D.discoideum calmodulin mRNA, partial ...   135   4e-31
CP001574_245(CP001574|pid:none) Micromonas sp. RCC299 chromosome...   124   1e-27
AB222133_1(AB222133|pid:none) Pan troglodytes verus calm1 mRNA f...   122   3e-27
BC010730_1(BC010730|pid:none) Mus musculus calmodulin 2, mRNA (c...   122   3e-27
AY891413_1(AY891413|pid:none) Synthetic construct Homo sapiens c...   122   3e-27
DQ479425_1(DQ479425|pid:none) Synthetic construct calcium sensor...   122   3e-27
DQ457005_1(DQ457005|pid:none) Synthetic construct plasmid pN1-4n...   122   3e-27
AK315694_1(AK315694|pid:none) Homo sapiens cDNA, FLJ96792, highl...   122   3e-27
AY894058_1(AY894058|pid:none) Synthetic construct Homo sapiens c...   122   3e-27
I49567(I49567) calmodulin - mouse  &M19381_1(M19381|pid:none)         122   3e-27
protein update 2009. 7.31
PSORT 

 psg: 0.75  gvh: 0.37  alm: 0.56  top: 0.53  tms: 0.00  mit: 0.09  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 32.0 %: cytoplasmic
	 16.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VSF215 is nuc
5' end seq. ID VSF215F
5' end seq. 
>VSF215F.Seq
GAAGAAGAAATCCGTGAAGCATTCAAAGTTTTCGATAAAGACGGTAACGGTTACATCTCA
GCTGCTGAACTTAGACACGTTATGACCAGTCTTGGTGAAAAACTTACCAACGAAGAAGTT
GATGAAATGATTAGAGAAGCTGATTTGGATGGCGATGGTCAAGTTAACTATGACGAATTT
GTTAAAATGATGATTGTTAGAAATTAAGAAAATTTAATATTAAAAAAAAACAAAAACCTT
GAAAAAAAAAAAAAAAAACCAAATTTTTTTTTTTTAANTAAAAAACCCCNTTTTTTTTAN
NGNAAAAAAAAA----------
Length of 5' end seq. 312
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -