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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSF196
Library VS
(Link to library)
Clone ID VSF196
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14518-1
Original site URL
Representative seq. ID VSF196E
(Link to Original site)
Representative DNA sequence 
>VSF196 (VSF196Q) /CSM/VS/VSF1-D/VSF196Q.Seq.d/
CACACACGTTGTTGTTTACCTAATTTATATTTATTTAATAAATATCATATTTGGAATATT
TACATATTACTAAATTTAAAATGGATGAAGATTATGAACAAACACTTTTAGTCAAAAAGG
AATGTTTTATTTATAGAATTCCACCAAGACCAAGTGCAAGTGGTTATAAAGCTCAAGATT
GGGATCCATCTTCCTTTATTTGGACTGGCAGATTAGTTATTGTAGCAAGAGGTGAAAATT
GTGTTATCAGATTTGAAGATCCAAATAATGGTGAAATCTTTGCACAATGCCCATTTGATC
AAAACTCTGTTGAACCAGTTGTCGATAGTAGTCGTTATTTTGTAATTAGAATTAAAGATG
GTGAACGTCATGCATTTGTTGGAATGGGTTTCACCGATAGAGATGATGCTTTTGAATTCA
ATGCAACTTTACAAGATCATCAAAATTATATTAAAAATAAAAAAGATATTGAAATTCAAA
GAAGAAATTATGAAAATGAACCAAAGAAAGATTATAGTTTAAAGACTGGTCAAACAATTC
ATATTCCATTTAAAGCACCAACTTCAAAAGCACCAGCTGGAAATAATAATAACAATAATA
ATAAAAAGAATTTACCAGTTTTAACAGGTGCAAGTGGTGGTGGTTTCCTTTTATCTCCAC
CTCCACCAGCTTCTTCAAAAACTATTCAAAAACCACCTCAACAAAATGATTTCTTCTCTT
CTCCACAACAAAATAATAATAATAACAATAATAATATTTTTGGAAATGACCCATTTAATT
TCAACTCTTCACCACAACAACCACCAACAACCACCACAACAACAACAACAACAAAATAAT
AATAATAGTAATAATAGAAATAATAATAACAGCTTTGATGGTTTTGGTGACTTTAGCGAT
TTTGGAAATAAGAGCAACAATAGCAACAATAATAGTTTTGGAAATAATAATAATAATGAT
AATAACAATTTTGGAAATAATAATAATAACAACCTCTTTTCACCACCACAACAACAACAA
CAACAACAACAACAACAACAACAAAATAATAATCAATNCGGTTTAACTA
sequence update 2001. 3.25
Translated Amino Acid sequence 
htllft*fifi**ISYLEYLHITKFKMDEDYEQTLLVKKECFIYRIPPRPSASGYKAQDW
DPSSFIWTGRLVIVARGENCVIRFEDPNNGEIFAQCPFDQNSVEPVVDSSRYFVIRIKDG
ERHAFVGMGFTDRDDAFEFNATLQDHQNYIKNKKDIEIQRRNYENEPKKDYSLKTGQTIH
IPFKAPTSKAPAGNNNNNNNKKNLPVLTGASGGGFLLSPPPPASSKTIQKPPQQNDFFSS
PQQNNNNNNNNIFGNDPFNFNSSPQQPPTTTTTTTTTK*******k***ql*wfw*l*rf
wk*eqq*qq**fwk*******qfwk****qplfttttttttttttttk**sxrfn
Translated Amino Acid sequence (All Frames) 
Frame A:
htrcclpnlylfnkyhiwniyillnlkwmkimnkhf*skrnvlfiefhqdqvqvviklki
gihlplfglad*ll*qevkivlsdlkiqimvkslhnahliktllnqlsivvvil*lelkm
vnvmhllewvspiemmllnsmqlykiikiilkikkilkfkeeimkmnqrkiiv*rlvkqf
ifhlkhqlqkhqleiiitiiikriyqf*qvqvvvvsfylhlhqllqklfknhlnkmissl
lhnkiiiitiiiflemthlistlhhnnhqqppqqqqqqnnnnsnnrnnnnsfdgfgdfsd
fgnksnnsnnnsfgnnnnndnnnfgnnnnnnlfsppqqqqqqqqqqqqnnnqxglt


Frame B:
thvvvyliyiyliniifgiftyy*i*ng*rl*tntfsqkgmfyl*nstktkckwl*ssrl
gsiflyldwqisycskr*klcyqi*rsk*w*nlctmpi*sklc*tscr**slfcn*n*rw
*tscicwngfhr*r*cf*iqcnftrsskly*k*kry*nskkkl*k*tkerl*fkdwsnns
ysi*stnfkstswk***q***keftsfnrckwwwfpfiststsffknyskttstk*fllf
sttk****q**yfwk*pi*fqlftttttnnhhnnnnnkiiiiviieiiitalmvlvtlai
leiratiatiivleiiiimiitileiiiittsfhhhnnnnnnnnnnnkiiinxv*l


Frame C:
htllft*fifi**ISYLEYLHITKFKMDEDYEQTLLVKKECFIYRIPPRPSASGYKAQDW
DPSSFIWTGRLVIVARGENCVIRFEDPNNGEIFAQCPFDQNSVEPVVDSSRYFVIRIKDG
ERHAFVGMGFTDRDDAFEFNATLQDHQNYIKNKKDIEIQRRNYENEPKKDYSLKTGQTIH
IPFKAPTSKAPAGNNNNNNNKKNLPVLTGASGGGFLLSPPPPASSKTIQKPPQQNDFFSS
PQQNNNNNNNNIFGNDPFNFNSSPQQPPTTTTTTTTTK*******k***ql*wfw*l*rf
wk*eqq*qq**fwk*******qfwk****qplfttttttttttttttk**sxrfn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSF196 (VSF196Q) /CSM/VS/VSF1-D/VSF196Q.Seq.d/                        894   0.0
VFH841 (VFH841Q) /CSM/VF/VFH8-B/VFH841Q.Seq.d/                        894   0.0
AFN416 (AFN416Q) /CSM/AF/AFN4-A/AFN416Q.Seq.d/                        894   0.0
SHG142 (SHG142Q) /CSM/SH/SHG1-B/SHG142Q.Seq.d/                        807   0.0
SFF462 (SFF462Q) /CSM/SF/SFF4-C/SFF462Q.Seq.d/                        515   e-145
VSF850 (VSF850Q) /CSM/VS/VSF8-C/VSF850Q.Seq.d/                        111   2e-23
SFI557 (SFI557Q) /CSM/SF/SFI5-C/SFI557Q.Seq.d/                         38   0.27
SFG593 (SFG593Q) /CSM/SF/SFG5-D/SFG593Q.Seq.d/                         38   0.27
AFL625 (AFL625Q) /CSM/AF/AFL6-B/AFL625Q.Seq.d/                         38   0.27
AFL523 (AFL523Q) /CSM/AF/AFL5-A/AFL523Q.Seq.d/                         38   0.27
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AE014819|AE014819.1 Plasmodium falciparum 3D7 chromosome 14 section 4 of 13 of the complete sequence.    46   7e-04  9
AL160211|AL160211.2 Human chromosome 14 DNA sequence BAC R-134J10 of library RPCI-11  from chromosome 14 of Homo sapiens (Human).    52   0.017  1
AL133239|AL133239.4 Human chromosome 14 DNA sequence BAC R-71I8 of library RPCI-11 from chromosome 14 of Homo sapiens (Human).    52   0.017  1
AE015936|AE015936.1 Clostridium tetani E88, section 1 of 10 of the complete genome.    36   0.033  11
AL844507|AL844507.1 Plasmodium falciparum chromosome 8.            50   0.067  1
AX346159|AX346159.1 Sequence 1230 from Patent WO0200928.           36   0.071  5
Z98551|Z98551.1 Plasmodium falciparum MAL3P6.                      32   0.095  10
CL024796|CL024796.1 CH216-20E18_Sp6.1 CH216 Silurana tropicalis genomic clone CH216-20E18, genomic survey sequence.    48   0.11   2
AC140767|AC140767.2 Rattus norvegicus clone CH230-118M23, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    36   0.15   2
AC139929|AC139929.2 Rattus norvegicus clone CH230-219C6, *** SEQUENCING IN PROGRESS ***, 54 unordered pieces.    36   0.15   2
dna update 2004. 3.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY223256_1(AY223256|pid:none) Schistosoma japonicum clone ZZD434...   155   2e-36
EZ000148_1(EZ000148|pid:none) TSA: Schistosoma japonicum SJCHGC0...   155   2e-36
FN314655_1(FN314655|pid:none) Schistosoma japonicum isolate Anhu...   155   2e-36
AK220236_1(AK220236|pid:none) Mus musculus mRNA for mFLJ00061 pr...   155   2e-36
FN319340_1(FN319340|pid:none) Schistosoma japonicum isolate Anhu...   155   2e-36
BC129007_1(BC129007|pid:none) Xenopus tropicalis hypothetical pr...   155   2e-36
BC072846_1(BC072846|pid:none) Xenopus laevis MGC80231 protein, m...   155   3e-36
BC079728_1(BC079728|pid:none) Xenopus laevis MGC83534 protein, m...   154   4e-36
BC054244_1(BC054244|pid:none) Xenopus laevis hypothetical protei...   154   4e-36
CR860591_1(CR860591|pid:none) Pongo abelii mRNA; cDNA DKFZp459G0...   154   5e-36
protein update 2009. 7.31
PSORT 

 psg: 0.37  gvh: 0.33  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VSF196 is nuc
5' end seq. ID VSF196F
5' end seq. 
>VSF196F.Seq
CACACACGTTGTTGTTTACCTAATTTATATTTATTTAATAAATATCATATTTGGAATATT
TACATATTACTAAATTTAAAATGGATGAAGATTATGAACAAACACTTTTAGTCAAAAAGG
AATGTTTTATTTATAGAATTCCACCAAGACCAAGTGCAAGTGGTTATAAAGCTCAAGATT
GGGATCCATCTTCCTTTATTTGGACTGGCAGATTAGTTATTGTAGCAAGAGGTGAAAATT
GTGTTATCAGATTTGAAGATCCAAATAATGGTGAAATCTTTGCACAATGCCCATTTGATC
AAAACTCTGTTGAACCAGTTGTCGATAGTAGTCGTTATTTTGTAATTAGAATTAAAGATG
GTGAACGTCATGCATTTGTTGGAATGGGTTTCACCGATAGAGATGATGCTTTTGAATTCA
ATGCAACTTTACAAGATCATCAAAATTATATTAAAAATAAAAAAGATATTGAAATTCAAA
GAAGAAATTATGAAAATGAACCAAAGAAAGATT----------
Length of 5' end seq. 513
3' end seq. ID VSF196Z
3' end seq. 
>VSF196Z.Seq
----------TAAAAAAGATATTGAAATTCAAAGAAGAAATTATGAAAATGAACCAAAGA
AAGATTATAGTTTAAAGACTGGTCAAACAATTCATATTCCATTTAAAGCACCAACTTCAA
AAGCACCAGCTGGAAATAATAATAACAATAATAATAAAAAGAATTTACCAGTTTTAACAG
GTGCAAGTGGTGGTGGTTTCCTTTTATCTCCACCTCCACCAGCTTCTTCAAAAACTATTC
AAAAACCACCTCAACAAAATGATTTCTTCTCTTCTCCACAACAAAATAATAATAATAACA
ATAATAATATTTTTGGAAATGACCCATTTAATTTCAACTCTTCACCACAACAACCACCAA
CAACCACCACAACAACAACAACAACAAAATAATAATAATAGTAATAATAGAAATAATAAT
AACAGCTTTGATGGTTTTGGTGACTTTAGCGATTTTGGAAATAAGAGCAACAATAGCAAC
AATAATAGTTTTGGAAATAATAATAATAATGATAATAACAATTTTGGAAATAATAATAAT
AACAACCTCTTTTCACCACCACAACAACAACAACAACAACAACAACAACAACAACAAAAT
AATAATCAATNCGGTTTAACTA
Length of 3' end seq. 612
Connected seq. ID VSF196P
Connected seq. 
>VSF196P.Seq
CACACACGTTGTTGTTTACCTAATTTATATTTATTTAATAAATATCATATTTGGAATATT
TACATATTACTAAATTTAAAATGGATGAAGATTATGAACAAACACTTTTAGTCAAAAAGG
AATGTTTTATTTATAGAATTCCACCAAGACCAAGTGCAAGTGGTTATAAAGCTCAAGATT
GGGATCCATCTTCCTTTATTTGGACTGGCAGATTAGTTATTGTAGCAAGAGGTGAAAATT
GTGTTATCAGATTTGAAGATCCAAATAATGGTGAAATCTTTGCACAATGCCCATTTGATC
AAAACTCTGTTGAACCAGTTGTCGATAGTAGTCGTTATTTTGTAATTAGAATTAAAGATG
GTGAACGTCATGCATTTGTTGGAATGGGTTTCACCGATAGAGATGATGCTTTTGAATTCA
ATGCAACTTTACAAGATCATCAAAATTATATTAAAAATAAAAAAGATATTGAAATTCAAA
GAAGAAATTATGAAAATGAACCAAAGAAAGATT----------TAAAAAAGATATTGAAA
TTCAAAGAAGAAATTATGAAAATGAACCAAAGAAAGATTATAGTTTAAAGACTGGTCAAA
CAATTCATATTCCATTTAAAGCACCAACTTCAAAAGCACCAGCTGGAAATAATAATAACA
ATAATAATAAAAAGAATTTACCAGTTTTAACAGGTGCAAGTGGTGGTGGTTTCCTTTTAT
CTCCACCTCCACCAGCTTCTTCAAAAACTATTCAAAAACCACCTCAACAAAATGATTTCT
TCTCTTCTCCACAACAAAATAATAATAATAACAATAATAATATTTTTGGAAATGACCCAT
TTAATTTCAACTCTTCACCACAACAACCACCAACAACCACCACAACAACAACAACAACAA
AATAATAATAATAGTAATAATAGAAATAATAATAACAGCTTTGATGGTTTTGGTGACTTT
AGCGATTTTGGAAATAAGAGCAACAATAGCAACAATAATAGTTTTGGAAATAATAATAAT
AATGATAATAACAATTTTGGAAATAATAATAATAACAACCTCTTTTCACCACCACAACAA
CAACAACAACAACAACAACAACAACAACAAAATAATAATCAATNCGGTTTAACTA
Length of connected seq. 1125
Full length Seq ID VSF196E
Full length Seq. 
>VSF196E.Seq
CACACACGTTGTTGTTTACCTAATTTATATTTATTTAATAAATATCATATTTGGAATATT
TACATATTACTAAATTTAAAATGGATGAAGATTATGAACAAACACTTTTAGTCAAAAAGG
AATGTTTTATTTATAGAATTCCACCAAGACCAAGTGCAAGTGGTTATAAAGCTCAAGATT
GGGATCCATCTTCCTTTATTTGGACTGGCAGATTAGTTATTGTAGCAAGAGGTGAAAATT
GTGTTATCAGATTTGAAGATCCAAATAATGGTGAAATCTTTGCACAATGCCCATTTGATC
AAAACTCTGTTGAACCAGTTGTCGATAGTAGTCGTTATTTTGTAATTAGAATTAAAGATG
GTGAACGTCATGCATTTGTTGGAATGGGTTTCACCGATAGAGATGATGCTTTTGAATTCA
ATGCAACTTTACAAGATCATCAAAATTATATTAAAAATAAAAAAGATATTGAAATTCAAA
GAAGAAATTATGAAAATGAACCAAAGAAAGATTATAGTTTAAAGACTGGTCAAACAATTC
ATATTCCATTTAAAGCACCAACTTCAAAAGCACCAGCTGGAAATAATAATAACAATAATA
ATAAAAAGAATTTACCAGTTTTAACAGGTGCAAGTGGTGGTGGTTTCCTTTTATCTCCAC
CTCCACCAGCTTCTTCAAAAACTATTCAAAAACCACCTCAACAAAATGATTTCTTCTCTT
CTCCACAACAAAATAATAATAATAACAATAATAATATTTTTGGAAATGACCCATTTAATT
TCAACTCTTCACCACAACAACCACCAACAACCACCACAACAACAACAACAACAAAATAAT
AATAATAGTAATAATAGAAATAATAATAACAGCTTTGATGGTTTTGGTGACTTTAGCGAT
TTTGGAAATAAGAGCAACAATAGCAACAATAATAGTTTTGGAAATAATAATAATAATGAT
AATAACAATTTTGGAAATAATAATAATAACAACCTCTTTTCACCACCACAACAACAACAA
CAACAACAACAACAACAACAACAAAATAATAATCAATNCGGTTTAACTA
Length of full length seq. 1069