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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSF195
Library VS
(Link to library)
Clone ID VSF195
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U14685-1
Original site URL
Representative seq. ID VSF195P
(Link to Original site)
Representative DNA sequence 
>VSF195 (VSF195Q) /CSM/VS/VSF1-D/VSF195Q.Seq.d/
TTAAAAAATAAAATAAAATAAATAAATAAAAAACTAATTATAAAAGTAATATAATAATTA
TTTATAATAAATAAATAAATAAATAAAATAAAAATAAAAAATATGGTTCTTAGTAAACAA
ATTGTATTAAAAAATCACATCGAAAGTGGTACACCAACTTTGGAGGATTTTGAAGAGAAA
ACTTTTGAATTTTCAATTGAAGAATTAAAAAATGAAGAAGTAGTAGTAAAATTAGAGATA
GTTTCACCAGACCCATATATGAGAGGTCGTATGACAACACGTAAATCATATATCCCACCA
TTTGAAATTGGTAAACCAATTGTTGGATATGCTATTGGTAAAGTTACAAAAGCAGGCTCA
GCATCAAAGTATTCAGTAGGTGATTACATTATGGGTCATTTACCATGGCAATCTGAATTC
ATCTACAATTCAAACAGTCCATATGCAAATAAAATCGATACAACATTAGCGCCATTGGAA
TCATTCCTCTCAGTTTTGGGTATGGTCXXXXXXXXXXATCGCAGAACCAAAACAAGGTGA
AACCATGGTAATCAGCGCTGCAGCCGGTGCAGTAGGTCAATTGGTCGGTCAAATTGGTAA
AATCAAAGGTTGCAGAGTTGTAGGTATCGTAGGTTCCGAAGAGAAGATAAAATACATCGT
CGATGAATTAGGTTTTGATGTCGGTGTAAATTACAATTCACCAACTTATAAAGACGATAT
AGCAAAAGCAATTCCAAATGGTGTTGATATATATTGGGAGAATGTTGGTGGTGTTGTATC
TGATGCAATTTGGCCACACTTGAATAAGTTTGCCCGTATTCCACTATGCGGTGTAATCTC
ACAATACAACTCTACTGAAAAGGATGTTGGCCCACGTATTGAAGGTTATCTCTTGAAAAC
TTCCTCAAAATTACAAGGTTTCATCGTTGCAAACTATGCAAGCAAACATGCTGAAGCATT
AAAAGAATTGGCTCAATGGTATAAATCTGGTCAACTCAAAGATAGACACACCATTAATAA
TGGTTTCGATCAATTGGTGCCATCTTTTCTTGCATTATTCAAAGGTACAAATACTGGTAA
AATGATGGTAAAATTGTAAAATAAATAAAAATAAAANCAATTNCTAAAAAA
sequence update 2001. 3.22
Translated Amino Acid sequence 
lknkik*inkkliikvi**lfiink*INKIKIKNMVLSKQIVLKNHIESGTPTLEDFEEK
TFEFSIEELKNEEVVVKLEIVSPDPYMRGRMTTRKSYIPPFEIGKPIVGYAIGKVTKAGS
ASKYSVGDYIMGHLPWQSEFIYNSNSPYANKIDTTLAPLESFLSVLGMV---

---IAEPKQGETMVISAAAGAVGQLVGQIGKIKGCRVVGIVGSEEKIKYIVDELGFDVGV
NYNSPTYKDDIAKAIPNGVDIYWENVGGVVSDAIWPHLNKFARIPLCGVISQYNSTEKDV
GPRIEGYLLKTSSKLQGFIVANYASKHAEALKELAQWYKSGQLKDRHTINNGFDQLVPSF
LALFKGTNTGKMMVKL*nk*k*xqxlk
Translated Amino Acid sequence (All Frames) 
Frame A:
lknkik*inkkliikvi**lfiink*INKIKIKNMVLSKQIVLKNHIESGTPTLEDFEEK
TFEFSIEELKNEEVVVKLEIVSPDPYMRGRMTTRKSYIPPFEIGKPIVGYAIGKVTKAGS
ASKYSVGDYIMGHLPWQSEFIYNSNSPYANKIDTTLAPLESFLSVLGMV---

---IAEPKQGETMVISAAAGAVGQLVGQIGKIKGCRVVGIVGSEEKIKYIVDELGFDVGV
NYNSPTYKDDIAKAIPNGVDIYWENVGGVVSDAIWPHLNKFARIPLCGVISQYNSTEKDV
GPRIEGYLLKTSSKLQGFIVANYASKHAEALKELAQWYKSGQLKDRHTINNGFDQLVPSF
LALFKGTNTGKMMVKL*nk*k*xqxlk

Frame B:
*kik*nk*ikn*l*k*ynnyl**ink*ik*k*kiwflvnkly*kitskvvhqlwrilkrk
llnfqlkn*kmkk***n*r*fhqthi*evv*qhvnhishhlklvnqlldmllvklqkqaq
hqsiq*vitlwviyhgnlnsstiqtvhmqiksiqh*rhwnhssqfwvw---

---sqnqnkvkpw*salqpvq*vnwsvklvkskvael*vs*vpkrr*ntssmn*vlmsv*
itihqlikti*qkqfqmvliyigrmlvvlylmqfght*islpvfhyav*shnttllkrml
ahvlkvis*klpqnykvsslqtmqanmlkh*knwlnginlvnskidtplimvsinwchlf
lhyskvqilvk*w*nckinknkxnx*k

Frame C:
kk*nkink*ktnyksniiiiynk*ink*nknkkygs**tncikkshrkwytnfggf*ren
f*ifn*rikk*rssskirdsftrpiyersydnt*iiypti*nw*tncwicyw*syksrls
ikvfsr*lhygsftmai*ihlqfkqsick*nrynisaigiiplsfgyg---

---rrtktr*nhgnqrcsrcsrsigrsnw*nqrlqscryrrfrredkihrr*irf*crck
lqftnl*rrysksnskwc*yilgecwwcci*cnlatle*vcpystmrcnltiqly*kgcw
pty*rlslenflkitrfhrcklckqtc*sikrigsmv*iwstqr*thh**wfrsigaifs
ciiqrykyw*ndgkivk*ikikxixkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSF195 (VSF195Q) /CSM/VS/VSF1-D/VSF195Q.Seq.d/                       1853   0.0
SSJ106 (SSJ106Q) /CSM/SS/SSJ1-A/SSJ106Q.Seq.d/                       1084   0.0
VHM751 (VHM751Q) /CSM/VH/VHM7-C/VHM751Q.Seq.d/                       1047   0.0
VSC130 (VSC130Q) /CSM/VS/VSC1-B/VSC130Q.Seq.d/                        969   0.0
VFM264 (VFM264Q) /CSM/VF/VFM2-C/VFM264Q.Seq.d/                        948   0.0
VSI692 (VSI692Q) /CSM/VS/VSI6-D/VSI692Q.Seq.d/                        626   e-178
SLD585 (SLD585Q) /CSM/SL/SLD5-D/SLD585Q.Seq.d/                        385   e-105
CHO803 (CHO803Q) /CSM/CH/CHO8-A/CHO803Q.Seq.d/                         76   1e-12
CHK326 (CHK326Q) /CSM/CH/CHK3-B/CHK326Q.Seq.d/                         76   1e-12
CHK301 (CHK301Q) /CSM/CH/CHK3-A/CHK301Q.Seq.d/                         76   1e-12
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AX622398|AX622398.1 Sequence 5361 from Patent WO02094868.         40  0.003  2
AF282920|AF282920.1 Staphylococcus aureus quinone oxidoreductase and glycine betaine transporter genes, complete cds.    40  0.007  3
AP003364|AP003364.2 Staphylococcus aureus subsp. aureus Mu50 genomic DNA, complete sequence, section 7/9.    40  0.030  9
AR354055|AR354055.1 Sequence 173 from patent US 6593114.          40  0.032  2
AP004829|AP004829.1 Staphylococcus aureus subsp. aureus MW2 DNA, complete genome, strain:MW2, section 8/10.    40  0.061  8
AC078831|AC078831.10 Staphylococcus aureus clone sabac-124, complete sequence.    40  0.066  2
AP003136|AP003136.2 Staphylococcus aureus subsp. aureus N315 genomic DNA, complete genome, section 8/10.    40  0.085  7
AC115606|AC115606.2 Dictyostelium discoideum chromosome 2 map 159760-209066 strain AX4, complete sequence.    36  0.13  4
AX144265|AX144265.1 Sequence 2987 from Patent WO0134809.          42  0.14  2
BQ134858|BQ134858.1 INIT1_4_H12.b1_A006 G5 trophont cDNA (INIT1) Ichthyophthirius multifiliis cDNA, mRNA sequence.    48  0.24  1
dna update 2003. 8.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BA000033_2113(BA000033|pid:none) Staphylococcus aureus subsp. au...   190   8e-65
DQ498909_1(DQ498909|pid:none) Staphylococcus xylosus NADP-depend...   191   3e-62
BA000028_2947(BA000028|pid:none) Oceanobacillus iheyensis HTE831...   172   1e-60
CP001022_791(CP001022|pid:none) Exiguobacterium sibiricum 255-15...   184   2e-60
AM295250_1683(AM295250|pid:none) Staphylococcus carnosus subsp. ...   189   2e-58
CP000431_5896(CP000431|pid:none) Rhodococcus jostii RHA1, comple...   156   4e-50
CU459140_14(CU459140|pid:none) Acinetobacter baumannii str. AYE ...   175   1e-49
AL939112_142(AL939112|pid:none) Streptomyces coelicolor A3(2) co...   142   3e-49
CP001037_1356(CP001037|pid:none) Nostoc punctiforme PCC 73102, c...   156   3e-49
BA000030_5783(BA000030|pid:none) Streptomyces avermitilis MA-468...   147   5e-49
protein update 2009. 7.31
PSORT 

 psg: 0.70  gvh: 0.25  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for VSF195 is cyt
5' end seq. ID VSF195F
5' end seq. 
>VSF195F.Seq
TTAAAAAATAAAATAAAATAAATAAATAAAAAACTAATTATAAAAGTAATATAATAATTA
TTTATAATAAATAAATAAATAAATAAAATAAAAATAAAAAATATGGTTCTTAGTAAACAA
ATTGTATTAAAAAATCACATCGAAAGTGGTACACCAACTTTGGAGGATTTTGAAGAGAAA
ACTTTTGAATTTTCAATTGAAGAATTAAAAAATGAAGAAGTAGTAGTAAAATTAGAGATA
GTTTCACCAGACCCATATATGAGAGGTCGTATGACAACACGTAAATCATATATCCCACCA
TTTGAAATTGGTAAACCAATTGTTGGATATGCTATTGGTAAAGTTACAAAAGCAGGCTCA
GCATCAAAGTATTCAGTAGGTGATTACATTATGGGTCATTTACCATGGCAATCTGAATTC
ATCTACAATTCAAACAGTCCATATGCAAATAAAATCGATACAACATTAGCGCCATTGGAA
TCATTCCTCTCAGTTTTGGGTATGGTC----------
Length of 5' end seq. 507
3' end seq. ID VSF195Z
3' end seq. 
>VSF195Z.Seq
----------ATCGCAGAACCAAAACAAGGTGAAACCATGGTAATCAGCGCTGCAGCCGG
TGCAGTAGGTCAATTGGTCGGTCAAATTGGTAAAATCAAAGGTTGCAGAGTTGTAGGTAT
CGTAGGTTCCGAAGAGAAGATAAAATACATCGTCGATGAATTAGGTTTTGATGTCGGTGT
AAATTACAATTCACCAACTTATAAAGACGATATAGCAAAAGCAATTCCAAATGGTGTTGA
TATATATTGGGAGAATGTTGGTGGTGTTGTATCTGATGCAATTTGGCCACACTTGAATAA
GTTTGCCCGTATTCCACTATGCGGTGTAATCTCACAATACAACTCTACTGAAAAGGATGT
TGGCCCACGTATTGAAGGTTATCTCTTGAAAACTTCCTCAAAATTACAAGGTTTCATCGT
TGCAAACTATGCAAGCAAACATGCTGAAGCATTAAAAGAATTGGCTCAATGGTATAAATC
TGGTCAACTCAAAGATAGACACACCATTAATAATGGTTTCGATCAATTGGTGCCATCTTT
TCTTGCATTATTCAAAGGTACAAATACTGGTAAAATGATGGTAAAATTGTAAAATAAATA
AAAATAAAANCAATTNCTAAAAAA
Length of 3' end seq. 614
Connected seq. ID VSF195P
Connected seq. 
>VSF195P.Seq
TTAAAAAATAAAATAAAATAAATAAATAAAAAACTAATTATAAAAGTAATATAATAATTA
TTTATAATAAATAAATAAATAAATAAAATAAAAATAAAAAATATGGTTCTTAGTAAACAA
ATTGTATTAAAAAATCACATCGAAAGTGGTACACCAACTTTGGAGGATTTTGAAGAGAAA
ACTTTTGAATTTTCAATTGAAGAATTAAAAAATGAAGAAGTAGTAGTAAAATTAGAGATA
GTTTCACCAGACCCATATATGAGAGGTCGTATGACAACACGTAAATCATATATCCCACCA
TTTGAAATTGGTAAACCAATTGTTGGATATGCTATTGGTAAAGTTACAAAAGCAGGCTCA
GCATCAAAGTATTCAGTAGGTGATTACATTATGGGTCATTTACCATGGCAATCTGAATTC
ATCTACAATTCAAACAGTCCATATGCAAATAAAATCGATACAACATTAGCGCCATTGGAA
TCATTCCTCTCAGTTTTGGGTATGGTC----------ATCGCAGAACCAAAACAAGGTGA
AACCATGGTAATCAGCGCTGCAGCCGGTGCAGTAGGTCAATTGGTCGGTCAAATTGGTAA
AATCAAAGGTTGCAGAGTTGTAGGTATCGTAGGTTCCGAAGAGAAGATAAAATACATCGT
CGATGAATTAGGTTTTGATGTCGGTGTAAATTACAATTCACCAACTTATAAAGACGATAT
AGCAAAAGCAATTCCAAATGGTGTTGATATATATTGGGAGAATGTTGGTGGTGTTGTATC
TGATGCAATTTGGCCACACTTGAATAAGTTTGCCCGTATTCCACTATGCGGTGTAATCTC
ACAATACAACTCTACTGAAAAGGATGTTGGCCCACGTATTGAAGGTTATCTCTTGAAAAC
TTCCTCAAAATTACAAGGTTTCATCGTTGCAAACTATGCAAGCAAACATGCTGAAGCATT
AAAAGAATTGGCTCAATGGTATAAATCTGGTCAACTCAAAGATAGACACACCATTAATAA
TGGTTTCGATCAATTGGTGCCATCTTTTCTTGCATTATTCAAAGGTACAAATACTGGTAA
AATGATGGTAAAATTGTAAAATAAATAAAAATAAAANCAATTNCTAAAAAA
Length of connected seq. 1121
Full length Seq ID -
Full length Seq. -
Length of full length seq. -