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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSF194
Library VS
(Link to library)
Clone ID VSF194
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VSF194P
(Link to Original site)
Representative DNA sequence 
>VSF194 (VSF194Q) /CSM/VS/VSF1-D/VSF194Q.Seq.d/
AGCTCACTACATAAAATGGAAAAGATTTACTCAAAAGGTGTCGTTTTAGGTTACAGAAGA
TCACAAGCTACTCAATACCCAAACATCAGTTTAATCAAAATTGAAGGTGTTGTCTCACGT
GAAGATTCAACTTTTTATTTAGGTAAAAAAGTCTGTTTAGTCAGCAAAGTTTCCAAATCA
GCCAAAAACCCAACTGGTCACAAAATTTCATGGGGTAAGATTTGCAAAACCCACGGTAGT
TCAGGTGTTGTCCAAGCTCGTTTCGCCAGAAACCTCCCACCAAAAGCTATGGGTGCCCCA
GTCAGAGTTATGTTATACCCATCAAACATCTAAATAAATCTTTAAAACGAAAAAAAAAAT
TATTTGAAAAAAAAAAXXXXXXXXXXAGCTCACTACATAAAATGGAAAAGATTTACTCAA
AAGGTGTCGTTTTAGGTTACAGAAGATCACAAGCTACTCAATACCCAAACATCAGTTTAA
TCAAAATTGAAGGTGTTGTCTCACGTGAAGATTCAACTTTTTATTTAGGTAAAAAAGTCT
GTTTAGTCAGCAAAGTTTCCAAATCAGCCAAAAACCCAACTGGTCACAAAATTTCATGGG
GTAAGATTTGCAAAACCCACGGTAGTTCAGGTGTTGTCCAAGCTCGTTTCGCCAGAAACC
TCCCACCAAAAGCTATGGGTGCCCCAGTCAGAGTTATGTTATACCCATCAAACATCTAAA
TAANNCTTAAAACGAAAAAAAAAA
sequence update 2001. 3.22
Translated Amino Acid sequence 
ltt*ngkdllkrcrfrlqkitsysipkhqfnqn*rcclt*rfnflfr*kslfsqqsfqis
qkpnwsqnfmg*dlqnpr*FRCCPSSFRQKPPTKSYGCPSQSYVIPIKHLNKSLKRKKKL
FEKK---

---SSLHKMEKIYSKGVVLGYRRSQATQYPNISLIKIEGVVSREDSTFYLGKKVCLVSKV
SKSAKNPTGHKISWGKICKTHGSSGVVQARFARNLPPKAMGAPVRVMLYPSNI*ixlktk
kk
Translated Amino Acid sequence (All Frames) 
Frame A:
sslhkmekiyskgvvlgyrrsqatqypnislikiegvvsredstfylgkkvclvskvsks
aknptghkiswgkickthgssgvvqarfarnlppkamgapvrvmlypsni*inl*nekkn
ylkkk---

---SSLHKMEKIYSKGVVLGYRRSQATQYPNISLIKIEGVVSREDSTFYLGKKVCLVSKV
SKSAKNPTGHKISWGKICKTHGSSGVVQARFARNLPPKAMGAPVRVMLYPSNI*ixlktk
kk

Frame B:
ahyikwkrftqkvsf*vtedhkllntqtsv*sklkvlshvkiqlfi*vkksv*sakfpnq
pktqlvtkfhgvrfakptvvqvlsklvspetshqklwvpqselcythqtsk*ifktkkki
i*kkk---

---ahyikwkrftqkvsf*vtedhkllntqtsv*sklkvlshvkiqlfi*vkksv*sakf
pnqpktqlvtkfhgvrfakptvvqvlsklvspetshqklwvpqselcythqtsk*xlkrk
kk

Frame C:
ltt*ngkdllkrcrfrlqkitsysipkhqfnqn*rcclt*rfnflfr*kslfsqqsfqis
qkpnwsqnfmg*dlqnpr*FRCCPSSFRQKPPTKSYGCPSQSYVIPIKHLNKSLKRKKKL
FEKK---

---ltt*ngkdllkrcrfrlqkitsysipkhqfnqn*rcclt*rfnflfr*kslfsqqsf
qisqkpnwsqnfmg*dlqnpr*frccpssfrqkpptksygcpsqsyvipikhlnxx*nek
k
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSK108 (VSK108Q) /CSM/VS/VSK1-A/VSK108Q.Seq.d/                        680   0.0
VSJ573 (VSJ573Q) /CSM/VS/VSJ5-D/VSJ573Q.Seq.d/                        680   0.0
VSJ462 (VSJ462Q) /CSM/VS/VSJ4-C/VSJ462Q.Seq.d/                        680   0.0
VSJ120 (VSJ120Q) /CSM/VS/VSJ1-A/VSJ120Q.Seq.d/                        680   0.0
VSH370 (VSH370Q) /CSM/VS/VSH3-C/VSH370Q.Seq.d/                        680   0.0
VSF827 (VSF827Q) /CSM/VS/VSF8-B/VSF827Q.Seq.d/                        680   0.0
VSF194 (VSF194Q) /CSM/VS/VSF1-D/VSF194Q.Seq.d/                        680   0.0
VSE875 (VSE875Q) /CSM/VS/VSE8-D/VSE875Q.Seq.d/                        680   0.0
VSD188 (VSD188Q) /CSM/VS/VSD1-D/VSD188Q.Seq.d/                        680   0.0
VSC278 (VSC278Q) /CSM/VS/VSC2-D/VSC278Q.Seq.d/                        680   0.0
own update 2004. 5.29
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BQ834795|BQ834795.1 Po_ad_03E06_TEXF1 Psoroptes ovis mixed Psoroptes ovis cDNA clone Po_ad_03E06 5' similar to BAB27124 ES CELLS CDNA, RIKEN FULL-LENGTH ENRICHE FULL INSERT, mRNA sequence.    36  0.15  2
BG211495|BG211495.1 RST31062 Athersys RAGE Library Homo sapiens cDNA, mRNA sequence.    38  0.75  2
D17450|D17450.1 Mycoplasma salivarium gene for aminopeptidase, complete cds.    34  1.7  2
AX344895|AX344895.1 Sequence 320 from Patent WO0200927.           40  4.6  2
AX323826|AX323826.1 Sequence 314 from Patent WO0192565.           40  4.6  2
BM023621|BM023621.1 ie82g07.y1 Melton Normalized Human Islet 4 N4-HIS 1 Homo sapiens cDNA clone IMAGE:5673421 5', mRNA sequence.    32  5.5  3
AP002899|AP002899.2 Oryza sativa genomic DNA, chromosome 1, PAC clone:P0456A01.    36  5.6  2
BF574666|BF574666.1 602132142F1 NIH_MGC_81 Homo sapiens cDNA clone IMAGE:4271554 5', mRNA sequence.    32  5.8  3
AP003142|AP003142.2 Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 1, PAC clone:P0435H01.    36  6.1  2
CD002652| ; putative ABC transporter; protein id: At4g04770.1, supported by cDNA: gi_15529142, supported by cDNA: gi_15912264 [Arabidopsis thaliana] [ E- value 8.00E-76 ], mRNA sequence.    36  6.2  2
dna update 2003. 8.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FB899478_1(FB899478|pid:none) Sequence 118751 from Patent WO2008...   103   6e-21
FB899466_1(FB899466|pid:none) Sequence 118739 from Patent WO2008...   103   7e-21
FJ362592_1(FJ362592|pid:none) Vernicia fordii 60S ribosomal prot...   103   7e-21
EF144854_1(EF144854|pid:none) Populus trichocarpa clone WS01119_...   103   7e-21
(Q9FZH0) RecName: Full=60S ribosomal protein L35a-2;  &AC008046_...   102   1e-20
FB899468_1(FB899468|pid:none) Sequence 118741 from Patent WO2008...   102   1e-20
(P51422) RecName: Full=60S ribosomal protein L35a-4;  &AF446895_...   102   1e-20
AY857450_1(AY857450|pid:none) Suberites domuncula L35a mRNA, com...   102   1e-20
(Q9C912) RecName: Full=60S ribosomal protein L35a-3;  &AC020579_...   102   2e-20
BT051287_1(BT051287|pid:none) Medicago truncatula clone MTYF1_F2...   101   3e-20
protein update 2009. 7.31
PSORT 

 psg: 0.70  gvh: 0.21  alm: 0.53  top: 0.53  tms: 0.00  mit: 0.48  mip: 0.07
 nuc: 0.20  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 36.0 %: mitochondrial
	 12.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for VSF194 is nuc
5' end seq. ID VSF194F
5' end seq. 
>VSF194F.Seq
AGCTCACTACATAAAATGGAAAAGATTTACTCAAAAGGTGTCGTTTTAGGTTACAGAAGA
TCACAAGCTACTCAATACCCAAACATCAGTTTAATCAAAATTGAAGGTGTTGTCTCACGT
GAAGATTCAACTTTTTATTTAGGTAAAAAAGTCTGTTTAGTCAGCAAAGTTTCCAAATCA
GCCAAAAACCCAACTGGTCACAAAATTTCATGGGGTAAGATTTGCAAAACCCACGGTAGT
TCAGGTGTTGTCCAAGCTCGTTTCGCCAGAAACCTCCCACCAAAAGCTATGGGTGCCCCA
GTCAGAGTTATGTTATACCCATCAAACATCTAAATAAATCTTTAAAACGAAAAAAAAAAT
TATTTGAAAAAAAAAA----------
Length of 5' end seq. 376
3' end seq. ID VSF194Z
3' end seq. 
>VSF194Z.Seq
----------AGCTCACTACATAAAATGGAAAAGATTTACTCAAAAGGTGTCGTTTTAGG
TTACAGAAGATCACAAGCTACTCAATACCCAAACATCAGTTTAATCAAAATTGAAGGTGT
TGTCTCACGTGAAGATTCAACTTTTTATTTAGGTAAAAAAGTCTGTTTAGTCAGCAAAGT
TTCCAAATCAGCCAAAAACCCAACTGGTCACAAAATTTCATGGGGTAAGATTTGCAAAAC
CCACGGTAGTTCAGGTGTTGTCCAAGCTCGTTTCGCCAGAAACCTCCCACCAAAAGCTAT
GGGTGCCCCAGTCAGAGTTATGTTATACCCATCAAACATCTAAATAANNCTTAAAACGAA
AAAAAAAA
Length of 3' end seq. 358
Connected seq. ID VSF194P
Connected seq. 
>VSF194P.Seq
AGCTCACTACATAAAATGGAAAAGATTTACTCAAAAGGTGTCGTTTTAGGTTACAGAAGA
TCACAAGCTACTCAATACCCAAACATCAGTTTAATCAAAATTGAAGGTGTTGTCTCACGT
GAAGATTCAACTTTTTATTTAGGTAAAAAAGTCTGTTTAGTCAGCAAAGTTTCCAAATCA
GCCAAAAACCCAACTGGTCACAAAATTTCATGGGGTAAGATTTGCAAAACCCACGGTAGT
TCAGGTGTTGTCCAAGCTCGTTTCGCCAGAAACCTCCCACCAAAAGCTATGGGTGCCCCA
GTCAGAGTTATGTTATACCCATCAAACATCTAAATAAATCTTTAAAACGAAAAAAAAAAT
TATTTGAAAAAAAAAA----------AGCTCACTACATAAAATGGAAAAGATTTACTCAA
AAGGTGTCGTTTTAGGTTACAGAAGATCACAAGCTACTCAATACCCAAACATCAGTTTAA
TCAAAATTGAAGGTGTTGTCTCACGTGAAGATTCAACTTTTTATTTAGGTAAAAAAGTCT
GTTTAGTCAGCAAAGTTTCCAAATCAGCCAAAAACCCAACTGGTCACAAAATTTCATGGG
GTAAGATTTGCAAAACCCACGGTAGTTCAGGTGTTGTCCAAGCTCGTTTCGCCAGAAACC
TCCCACCAAAAGCTATGGGTGCCCCAGTCAGAGTTATGTTATACCCATCAAACATCTAAA
TAANNCTTAAAACGAAAAAAAAAA
Length of connected seq. 734
Full length Seq ID -
Full length Seq. -
Length of full length seq. -