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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSF189
Library VS
(Link to library)
Clone ID VSF189
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U06683-1
Original site URL
Representative seq. ID VSF189P
(Link to Original site)
Representative DNA sequence 
>VSF189 (VSF189Q) /CSM/VS/VSF1-D/VSF189Q.Seq.d/
CAAGTGGTATTGGTGCTTGGGGTGGATCTGGTACATGTTATCCAATTTTTTCAGATTTTT
GTACATGTATTTTTGATAAACCATTAGAATTATGTGAACCATACAGAAAAGATTATTTAG
AATGTCTCCATAATGTTAAAGAATATAAGAGTCAATCATCAGAAAATTACCATAAAGGTG
GTGAGGAAAGAATTAACAAAGTTAGAGAAGCAATTAAAAGAGCTCAAGAATATGATCCAA
AAAAAGGAAAATCTGAATAAATTCAATTTTTTTTTTAATAGTAATAAATAATAATAATTA
ATAACTAATAATAATAATAATAATAATATAATTTAGTGATAATACGATAAAACACAAACA
ACAAAAAAAAAAXXXXXXXXXXCAAGTGGTATTGGTGCTTGGGGTGGATCTGGTACATGT
TATCCAATTTTTTCAGATTTTTGTACATGTATTTTTGATAAACCATTAGAATTATGTGAA
CCATACAGAAAAGATTATTTAGAATGTCTCCATAATGTTAAAGAATATAAGAGTCAATCA
TCAGAAAATTACCATAAAGGTGGTGAGGAAAGAATTAACAAAGTTAGAGAAGCAATTNAA
AGAGCT
sequence update 2001. 3.22
Translated Amino Acid sequence 
kwywclgwiwymlsnffrflymyf**tirim*tiqkrlfrmsp*c*ri*esiirklp*rw
*gkn*qs*rsn*kssri*skkrki*insifflivinnnn**liiiiiii*FSDNTIKHKQ
QKK---

---SGIGAWGGSGTCYPIFSDFCTCIFDKPLELCEPYRKDYLECLHNVKEYKSQSSENYH
KGGEERINKVREAIXRA
Translated Amino Acid sequence (All Frames) 
Frame A:
qvvlvlgvdlvhviqffqifvhvflinh*nyvnhtekii*nvsimlknirvnhqkitikv
vrkeltklekqlkelknmiqkkenlnkfnfffnsnk***litnnnnnnnii***ydktqt
tkkk---

---qvvlvlgvdlvhviqffqifvhvflinh*nyvnhtekii*nvsimlknirvnhqkit
ikvvrkeltklekqxke

Frame B:
kwywclgwiwymlsnffrflymyf**tirim*tiqkrlfrmsp*c*ri*esiirklp*rw
*gkn*qs*rsn*kssri*skkrki*insifflivinnnn**liiiiiii*FSDNTIKHKQ
QKK---

---kwywclgwiwymlsnffrflymyf**tirim*tiqkrlfrmsp*c*ri*esiirklp
*rw*gkn*qs*rsnxks

Frame C:
sgigawggsgtcypifsdfctcifdkplelcepyrkdyleclhnvkeyksqssenyhkgg
eerinkvreaikraqeydpkkgkse*iqfff****iiiinn*******ynlviir*ntnn
kkk---

---SGIGAWGGSGTCYPIFSDFCTCIFDKPLELCEPYRKDYLECLHNVKEYKSQSSENYH
KGGEERINKVREAIXRA
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI449 (VSI449Q) /CSM/VS/VSI4-C/VSI449Q.Seq.d/                        472   e-132
VSF189 (VSF189Q) /CSM/VS/VSF1-D/VSF189Q.Seq.d/                        472   e-132
VSG455 (VSG455Q) /CSM/VS/VSG4-C/VSG455Q.Seq.d/                        234   1e-60
VHQ456 (VHQ456Q) /CSM/VH/VHQ4-C/VHQ456Q.Seq.d/                         34   2.4
VHH662 (VHH662Q) /CSM/VH/VHH6-C/VHH662Q.Seq.d/                         34   2.4
VHG211 (VHG211Q) /CSM/VH/VHG2-A/VHG211Q.Seq.d/                         34   2.4
VHC465 (VHC465Q) /CSM/VH/VHC4-C/VHC465Q.Seq.d/                         34   2.4
VHB296 (VHB296Q) /CSM/VH/VHB2-D/VHB296Q.Seq.d/                         34   2.4
VFK306 (VFK306Q) /CSM/VF/VFK3-A/VFK306Q.Seq.d/                         34   2.4
VFH740 (VFH740Q) /CSM/VF/VFH7-B/VFH740Q.Seq.d/                         34   2.4
own update 2004. 8. 6
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AL671910|AL671910.9 Mouse DNA sequence from clone RP23-245M12 on chromosome X.    46  0.053  3
AF528383|AF528383.1 Hypochaeris sessiliflora isolate 1 chloroplast tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer region, partial sequence.    38  0.14  2
AC025726|AC025726.2 Caenorhabditis elegans cosmid Y71G12B, complete sequence.    46  0.30  3
AC006895|AC006895.2 Caenorhabditis elegans clone Y71G12, WORKING DRAFT SEQUENCE, 12 unordered pieces.    46  0.33  3
BG618291|BG618291.1 602645836F1 NIH_MGC_76 Homo sapiens cDNA clone IMAGE:4767417 5', mRNA sequence.    40  0.51  2
AC100776|AC100776.2 Homo sapiens chromosome 18, clone CTD-2009D8, complete sequence.    38  0.54  2
AC110952|AC110952.6 Rattus norvegicus clone CH230-62F23, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    38  0.56  4
AC096799|AC096799.6 Rattus norvegicus clone CH230-94K2, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    38  0.68  4
AC018876|AC018876.4 Homo sapiens chromosome 18 clone RP11-289E15, WORKING DRAFT SEQUENCE, 8 unordered pieces.    38  0.81  3
AC008480|AC008480.4 Homo sapiens chromosome 5 clone CTC-397O13, WORKING DRAFT SEQUENCE, 5 unordered pieces.    42  0.83  2
dna update 2003. 8.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EF084615_1(EF084615|pid:none) Picea sitchensis clone WS02714_K03...    50   5e-05
AK121358_1(AK121358|pid:none) Oryza sativa Japonica Group cDNA c...    49   1e-04
CP001327_171(CP001327|pid:none) Micromonas sp. RCC299 chromosome...    48   2e-04
EF144605_1(EF144605|pid:none) Populus trichocarpa clone WS01111_...    47   4e-04
AC005309_6(AC005309|pid:none) Arabidopsis thaliana chromosome 2 ...    46   7e-04
AY088460_1(AY088460|pid:none) Arabidopsis thaliana clone 6853 mR...    46   7e-04
CR954212_50(CR954212|pid:none) Ostreococcus tauri strain OTTH059...    44   0.003
AL353822_11(AL353822|pid:none) Neurospora crassa DNA linkage gro...    39   0.11
CR382132_764(CR382132|pid:none) Yarrowia lipolytica strain CLIB1...    37   0.57
DQ091254_1(DQ091254|pid:none) Fenneropenaeus chinensis thrombosp...    35   1.6
protein update 2009. 7.31
PSORT 

 psg: 0.55  gvh: 0.42  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 32.0 %: nuclear
	 20.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VSF189 is cyt
5' end seq. ID VSF189F
5' end seq. 
>VSF189F.Seq
CAAGTGGTATTGGTGCTTGGGGTGGATCTGGTACATGTTATCCAATTTTTTCAGATTTTT
GTACATGTATTTTTGATAAACCATTAGAATTATGTGAACCATACAGAAAAGATTATTTAG
AATGTCTCCATAATGTTAAAGAATATAAGAGTCAATCATCAGAAAATTACCATAAAGGTG
GTGAGGAAAGAATTAACAAAGTTAGAGAAGCAATTAAAAGAGCTCAAGAATATGATCCAA
AAAAAGGAAAATCTGAATAAATTCAATTTTTTTTTTAATAGTAATAAATAATAATAATTA
ATAACTAATAATAATAATAATAATAATATAATTTAGTGATAATACGATAAAACACAAACA
ACAAAAAAAAAA----------
Length of 5' end seq. 372
3' end seq. ID VSF189Z
3' end seq. 
>VSF189Z.Seq
----------CAAGTGGTATTGGTGCTTGGGGTGGATCTGGTACATGTTATCCAATTTTT
TCAGATTTTTGTACATGTATTTTTGATAAACCATTAGAATTATGTGAACCATACAGAAAA
GATTATTTAGAATGTCTCCATAATGTTAAAGAATATAAGAGTCAATCATCAGAAAATTAC
CATAAAGGTGGTGAGGAAAGAATTAACAAAGTTAGAGAAGCAATTNAAAGAGCT
Length of 3' end seq. 224
Connected seq. ID VSF189P
Connected seq. 
>VSF189P.Seq
CAAGTGGTATTGGTGCTTGGGGTGGATCTGGTACATGTTATCCAATTTTTTCAGATTTTT
GTACATGTATTTTTGATAAACCATTAGAATTATGTGAACCATACAGAAAAGATTATTTAG
AATGTCTCCATAATGTTAAAGAATATAAGAGTCAATCATCAGAAAATTACCATAAAGGTG
GTGAGGAAAGAATTAACAAAGTTAGAGAAGCAATTAAAAGAGCTCAAGAATATGATCCAA
AAAAAGGAAAATCTGAATAAATTCAATTTTTTTTTTAATAGTAATAAATAATAATAATTA
ATAACTAATAATAATAATAATAATAATATAATTTAGTGATAATACGATAAAACACAAACA
ACAAAAAAAAAA----------CAAGTGGTATTGGTGCTTGGGGTGGATCTGGTACATGT
TATCCAATTTTTTCAGATTTTTGTACATGTATTTTTGATAAACCATTAGAATTATGTGAA
CCATACAGAAAAGATTATTTAGAATGTCTCCATAATGTTAAAGAATATAAGAGTCAATCA
TCAGAAAATTACCATAAAGGTGGTGAGGAAAGAATTAACAAAGTTAGAGAAGCAATTNAA
AGAGCT
Length of connected seq. 596
Full length Seq ID -
Full length Seq. -
Length of full length seq. -