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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSF154
Library VS
(Link to library)
Clone ID VSF154
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15035-1
Original site URL
Representative seq. ID VSF154P
(Link to Original site)
Representative DNA sequence 
>VSF154 (VSF154Q) /CSM/VS/VSF1-C/VSF154Q.Seq.d/
ATTATCATCAAGAGGTGATGAAGATCCAGCTCAAGTTGATGAATCAAAGAATATAATTGA
AACTATTATCAAACATGAAATGGAAGAAGAGAAAATTCCAGCTGAAAGAATTATAATTGG
TGGTTTTTCACAAGGTGCAGCATTATCATTATATACATTTTATTCACAAACTGAAACCAA
ATTAGGTGGTTGTATTGCATTATCAGGTTATTTACCATTGGCAACTAAATTTGTAGCAAA
TTCATTAAATAAAGAACAACCATTACTCATGATCCACGGTGATTGTGATCAAGTAGTTCG
TCATCAATGGGGTAAATTATCATTCGACCATTTAAAAAGTCAAGGTATTAATGGTGAATT
TATAACATTAAAAGGTTTAGGTCATCATTCAAGTCCAGAGGAAATTGATTTAATGACAAA
GTTTATTTCTAAAACATTACCAAAATAAAAATAAAAATAAAAATAAAAATAAAAATAATA
AAAAAAAAAXXXXXXXXXXATTATCATCAAGAGGTGATGAAGATCCAGCTCAAGTTGATG
AATCAAAGAATATAATTGAAACTATTATCAAACATGAAATGGAAGAAGAGAAAATTCCAG
CTGAAAGAATTATAATTGGTGGTTTTTCACAAGGTGCAGCATTATCATTATATACATTTT
ATTCACAAACTGAAACCAAATTAGGTGGTTGTATTGCATTATCAGGTTATTTACCATTGG
CAACTAAATTTGTAGCAAATTCATTAAATAAAGAACAACCATTACTCATGATCCACGGTG
ATTGTGATCAAGTAGTTCGTCATCAATGGGGTAAATTATCATTCGACCATTTAAAAAGTC
AAGGTATTAATGGTGAATTTATAACATTAAAAGGTTTAGGTCATCATTCAAGTCCAGAGG
AAATTTATTTAATGACAAAGTTTATTTCTAAAACATTACCAAAATAAAAATAAAAAAAAA
A
sequence update 2001. 3.22
Translated Amino Acid sequence 
yhqevmkiqlklmnqri*lkllsnmkwkkrkfqlkel*lvvfhkvqhyhyihfihklkpn
*vvvlhyqviyhwqlnl*qih*iknnhys*stvivik*fvingvnyhsti*kvkvlmvnl
*h*kv*viiqvqrkli**QSLFLKHYQNKNKNKNKNKNNKKK---

---LSSRGDEDPAQVDESKNIIETIIKHEMEEEKIPAERIIIGGFSQGAALSLYTFYSQT
ETKLGGCIALSGYLPLATKFVANSLNKEQPLLMIHGDCDQVVRHQWGKLSFDHLKSQGIN
GEFITLKGLGHHSSPEEIYLMTKFISKTLPK*k*kk
Translated Amino Acid sequence (All Frames) 
Frame A:
iiikr**rssss**ikeyn*nyyqt*ngrrenss*knynwwfftrcsiiiiyilftn*nq
irwlyciirlftign*icskfik*rttithdpr*l*ssssssmg*iiirpfkksry*w*i
ynikrfrssfksrgn*fndkvyf*nitkikikikikikiikkk---

---iiikr**rssss**ikeyn*nyyqt*ngrrenss*knynwwfftrcsiiiiyilftn
*nqirwlyciirlftign*icskfik*rttithdpr*l*ssssssmg*iiirpfkksry*
w*iynikrfrssfksrgnlfndkvyf*nitkikikkk

Frame B:
lssrgdedpaqvdeskniietiikhemeeekipaeriiiggfsqgaalslytfysqtetk
lggcialsgylplatkfvanslnkeqpllmihgdcdqvvrhqwgklsfdhlksqgingef
itlkglghhsspeeidlmtkfisktlpk*k*k*k*k*k**kk---

---LSSRGDEDPAQVDESKNIIETIIKHEMEEEKIPAERIIIGGFSQGAALSLYTFYSQT
ETKLGGCIALSGYLPLATKFVANSLNKEQPLLMIHGDCDQVVRHQWGKLSFDHLKSQGIN
GEFITLKGLGHHSSPEEIYLMTKFISKTLPK*k*kk

Frame C:
yhqevmkiqlklmnqri*lkllsnmkwkkrkfqlkel*lvvfhkvqhyhyihfihklkpn
*vvvlhyqviyhwqlnl*qih*iknnhys*stvivik*fvingvnyhsti*kvkvlmvnl
*h*kv*viiqvqrkli**QSLFLKHYQNKNKNKNKNKNNKKK---

---yhqevmkiqlklmnqri*lkllsnmkwkkrkfqlkel*lvvfhkvqhyhyihfihkl
kpn*vvvlhyqviyhwqlnl*qih*iknnhys*stvivik*fvingvnyhsti*kvkvlm
vnl*h*kv*viiqvqrkfi**qslflkhyqnknkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI690 (VSI690Q) /CSM/VS/VSI6-D/VSI690Q.Seq.d/                        874   0.0
VSF154 (VSF154Q) /CSM/VS/VSF1-C/VSF154Q.Seq.d/                        874   0.0
AHE104 (AHE104Q) /CSM/AH/AHE1-A/AHE104Q.Seq.d/                        837   0.0
SSA104 (SSA104Q) /CSM/SS/SSA1-A/SSA104Q.Seq.d/                        803   0.0
SSJ323 (SSJ323Q) /CSM/SS/SSJ3-A/SSJ323Q.Seq.d/                        737   0.0
FC-BJ08 (FC-BJ08Q) /CSM/FC/FC-BJ/FC-BJ08Q.Seq.d/                      605   e-172
VSJ183 (VSJ183Q) /CSM/VS/VSJ1-D/VSJ183Q.Seq.d/                         38   0.24
SFJ480 (SFJ480Q) /CSM/SF/SFJ4-D/SFJ480Q.Seq.d/                         38   0.24
FC-AA12 (FC-AA12Q) /CSM/FC/FC-AA/FC-AA12Q.Seq.d/                       38   0.24
VHN571 (VHN571Q) /CSM/VH/VHN5-C/VHN571Q.Seq.d/                         36   0.96
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AI888811|AI888811.1 wn36h04.x1 NCI_CGAP_Gas4 Homo sapiens cDNA clone IMAGE:2447575 3', mRNA sequence.    36  0.053  2
BF298121|BF298121.1 060PbE12 Pb cDNA #17, Tommaso Pace, Marta Ponzi, and Clara Frontali Plasmodium berghei cDNA 5', mRNA sequence.    38  0.096  3
BF296904|BF296904.1 044PbC10 Pb cDNA #17, Tommaso Pace, Marta Ponzi, and Clara Frontali Plasmodium berghei cDNA 5', mRNA sequence.    38  0.096  3
BF298173|BF298173.1 061PbC05 Pb cDNA #17, Tommaso Pace, Marta Ponzi, and Clara Frontali Plasmodium berghei cDNA 5', mRNA sequence.    38  0.098  3
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    32  0.26  14
AP002462|AP002462.1 Arabidopsis thaliana genomic DNA, chromosome 3, BAC clone:T8N9.    38  0.76  2
AQ193319|AQ193319.1 HS_3065_A1_A12_MF CIT Approved Human Genomic Sperm Library D Homo sapiens genomic clone Plate=3065 Col=23 Row=A, genomic survey sequence.    34  0.76  2
AZ536609|AZ536609.1 Gm_ISb001_031_N03_R ISU Soybean BAC Library (pBeloBAC11 HindIII) Glycine max genomic clone Gm_ISb001_031_N03, DNA sequence.    34  0.96  2
AQ956307|AQ956307.1 LERAJ24TF LERA Arabidopsis thaliana genomic clone LERAJ24, genomic survey sequence.    38  1.1  2
L40815|L40815.1 Plasmodium berghei heat shock protein 70 (hsp70) gene, complete cds.    38  1.6  3
dna update 2003. 8.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54T49) RecName: Full=Acyl-protein thioesterase 1 homolog 1;   ...   299   8e-80
T52511(T52511)related to lysophospholipase [imported] - Neurospo...   108   3e-22
CU633899_555(CU633899|pid:none) Podospora anserina genomic DNA c...    99   2e-19
BC073342_1(BC073342|pid:none) Xenopus laevis MGC80756 protein, m...    99   3e-19
(Q55FK4) RecName: Full=Acyl-protein thioesterase 1 homolog 2;   ...    99   3e-19
BC064187_1(BC064187|pid:none) Xenopus tropicalis hypothetical pr...    97   1e-18
CR761348_1(CR761348|pid:none) Xenopus tropicalis finished cDNA, ...    96   2e-18
BT044647_1(BT044647|pid:none) Salmo salar clone ssal-rgf-001-321...    94   5e-18
(O77821) RecName: Full=Acyl-protein thioesterase 1;          EC=...    94   7e-18
BT048041_1(BT048041|pid:none) Salmo salar clone ssal-evd-522-113...    94   7e-18
protein update 2009. 7.31
PSORT 

 psg: 0.19  gvh: 0.08  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.06
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for VSF154 is nuc
5' end seq. ID VSF154F
5' end seq. 
>VSF154F.Seq
ATTATCATCAAGAGGTGATGAAGATCCAGCTCAAGTTGATGAATCAAAGAATATAATTGA
AACTATTATCAAACATGAAATGGAAGAAGAGAAAATTCCAGCTGAAAGAATTATAATTGG
TGGTTTTTCACAAGGTGCAGCATTATCATTATATACATTTTATTCACAAACTGAAACCAA
ATTAGGTGGTTGTATTGCATTATCAGGTTATTTACCATTGGCAACTAAATTTGTAGCAAA
TTCATTAAATAAAGAACAACCATTACTCATGATCCACGGTGATTGTGATCAAGTAGTTCG
TCATCAATGGGGTAAATTATCATTCGACCATTTAAAAAGTCAAGGTATTAATGGTGAATT
TATAACATTAAAAGGTTTAGGTCATCATTCAAGTCCAGAGGAAATTGATTTAATGACAAA
GTTTATTTCTAAAACATTACCAAAATAAAAATAAAAATAAAAATAAAAATAAAAATAATA
AAAAAAAAA----------
Length of 5' end seq. 489
3' end seq. ID VSF154Z
3' end seq. 
>VSF154Z.Seq
----------ATTATCATCAAGAGGTGATGAAGATCCAGCTCAAGTTGATGAATCAAAGA
ATATAATTGAAACTATTATCAAACATGAAATGGAAGAAGAGAAAATTCCAGCTGAAAGAA
TTATAATTGGTGGTTTTTCACAAGGTGCAGCATTATCATTATATACATTTTATTCACAAA
CTGAAACCAAATTAGGTGGTTGTATTGCATTATCAGGTTATTTACCATTGGCAACTAAAT
TTGTAGCAAATTCATTAAATAAAGAACAACCATTACTCATGATCCACGGTGATTGTGATC
AAGTAGTTCGTCATCAATGGGGTAAATTATCATTCGACCATTTAAAAAGTCAAGGTATTA
ATGGTGAATTTATAACATTAAAAGGTTTAGGTCATCATTCAAGTCCAGAGGAAATTTATT
TAATGACAAAGTTTATTTCTAAAACATTACCAAAATAAAAATAAAAAAAAAA
Length of 3' end seq. 462
Connected seq. ID VSF154P
Connected seq. 
>VSF154P.Seq
ATTATCATCAAGAGGTGATGAAGATCCAGCTCAAGTTGATGAATCAAAGAATATAATTGA
AACTATTATCAAACATGAAATGGAAGAAGAGAAAATTCCAGCTGAAAGAATTATAATTGG
TGGTTTTTCACAAGGTGCAGCATTATCATTATATACATTTTATTCACAAACTGAAACCAA
ATTAGGTGGTTGTATTGCATTATCAGGTTATTTACCATTGGCAACTAAATTTGTAGCAAA
TTCATTAAATAAAGAACAACCATTACTCATGATCCACGGTGATTGTGATCAAGTAGTTCG
TCATCAATGGGGTAAATTATCATTCGACCATTTAAAAAGTCAAGGTATTAATGGTGAATT
TATAACATTAAAAGGTTTAGGTCATCATTCAAGTCCAGAGGAAATTGATTTAATGACAAA
GTTTATTTCTAAAACATTACCAAAATAAAAATAAAAATAAAAATAAAAATAAAAATAATA
AAAAAAAAA----------ATTATCATCAAGAGGTGATGAAGATCCAGCTCAAGTTGATG
AATCAAAGAATATAATTGAAACTATTATCAAACATGAAATGGAAGAAGAGAAAATTCCAG
CTGAAAGAATTATAATTGGTGGTTTTTCACAAGGTGCAGCATTATCATTATATACATTTT
ATTCACAAACTGAAACCAAATTAGGTGGTTGTATTGCATTATCAGGTTATTTACCATTGG
CAACTAAATTTGTAGCAAATTCATTAAATAAAGAACAACCATTACTCATGATCCACGGTG
ATTGTGATCAAGTAGTTCGTCATCAATGGGGTAAATTATCATTCGACCATTTAAAAAGTC
AAGGTATTAATGGTGAATTTATAACATTAAAAGGTTTAGGTCATCATTCAAGTCCAGAGG
AAATTTATTTAATGACAAAGTTTATTTCTAAAACATTACCAAAATAAAAATAAAAAAAAA
A
Length of connected seq. 951
Full length Seq ID -
Full length Seq. -
Length of full length seq. -