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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSF149
Library VS
(Link to library)
Clone ID VSF149
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14763-1
Original site URL
Representative seq. ID VSF149P
(Link to Original site)
Representative DNA sequence 
>VSF149 (VSF149Q) /CSM/VS/VSF1-C/VSF149Q.Seq.d/
CGGAACGCGTGGGCAAAGAAGCTTCTTTGTAAATAATTTAATTTTCTTATTATATCTTTT
TCTTTTTTTTTTTTAAAAAAAAAACCTTAACCCAAAAAAAAAAXXXXXXXXXXGTNGGTG
GCTACGGTNATGGAGGTGGCCGTNAGGGATGGAGTTCATNATCAATNTCTTACTTAATAC
ANC
sequence update 2001. 3.22
Translated Amino Acid sequence 
ervgkeasl*ii*FSYYIFFFFFFKKKTLTQKK---

---VGGYGXGGGRXGWSSXSXSYLIX
Translated Amino Acid sequence (All Frames) 
Frame A:
rnawakkllck*fnfliisfsffflkkkp*pkkk---

---VGGYGXGGGRXGWSSXSXSYLIX

Frame B:
gtrgqrsffvnnlifllylflfff*kknlnpkkk---

---xvatvmevavrdgvhxqxlt*yx

Frame C:
ervgkeasl*ii*FSYYIFFFFFFKKKTLTQKK---

---xwlrxwrwpxgmefxinxllnt
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSF149 (VSF149Q) /CSM/VS/VSF1-C/VSF149Q.Seq.d/                        111   4e-24
VSK326 (VSK326Q) /CSM/VS/VSK3-B/VSK326Q.Seq.d/                         90   1e-17
VSI349 (VSI349Q) /CSM/VS/VSI3-C/VSI349Q.Seq.d/                         90   1e-17
VSE449 (VSE449Q) /CSM/VS/VSE4-C/VSE449Q.Seq.d/                         90   1e-17
VSH162 (VSH162Q) /CSM/VS/VSH1-C/VSH162Q.Seq.d/                         82   3e-15
CHR704 (CHR704Q) /CSM/CH/CHR7-A/CHR704Q.Seq.d/                         38   0.043
VHB710 (VHB710Q) /CSM/VH/VHB7-A/VHB710Q.Seq.d/                         36   0.17
SHL690 (SHL690Q) /CSM/SH/SHL6-D/SHL690Q.Seq.d/                         36   0.17
SHE140 (SHE140Q) /CSM/SH/SHE1-B/SHE140Q.Seq.d/                         36   0.17
CHE781 (CHE781Q) /CSM/CH/CHE7-D/CHE781Q.Seq.d/                         36   0.17
own update 2003. 7. 6
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BX601648|BX601648.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    44  0.55  1
BI228838|BI228838.1 RE26485.5prime RE Drosophila melanogaster normalized Embryo pFlc-1 Drosophila melanogaster cDNA clone RE26485 5 similar to Crk: FBan0001587 'signal transduction' located on: 4 102A8-102A8;: 04/12/2001, mRNA sequence.    44  0.55  1
AC068594|AC068594.15 Homo sapiens chromosome 17, clone RP11-285E9, complete sequence.    44  0.55  1
AC014640|AC014640.1 Drosophila melanogaster, WORKING DRAFT SEQUENCE, in ordered pieces.    44  0.55  1
AC010839|AC010839.6 Drosophila melanogaster, chromosome 4, region 101F-102F, BAC clone BACN19N21, complete sequence.    44  0.55  1
AC114394|AC114394.1 Drosophila melanogaster, chromosome 4, region 101B-101C, BAC clone BACR33O09, complete sequence.    44  0.55  1
AE003844|AE003844.4 Drosophila melanogaster chromosome 4 section 2 of 5 of the complete sequence.    44  0.55  1
AC011613|AC011613.4 Drosophila melanogaster, chromosome 4, region 101F-102F, BAC clone BACN04K08, complete sequence.    44  0.55  1
AC068169|AC068169.9 Homo sapiens chromosome 17 clone RP11-651B2 map 17, 2 ordered pieces.    44  0.55  1
AC145839|AC145839.2 Microcebus murinus clone CH257-408H17, WORKING DRAFT SEQUENCE, 4 ordered pieces.    36  0.56  2
dna update 2003. 8.23
Homology vs Protein 

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3268448
Number of Hits to DB: 146,952,335
protein update 2009. 7.31
PSORT 

 psg: 1.01  gvh: 0.43  alm: 0.50  top: 0.80  tms: 0.00  mit: 0.32  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: mitochondrial
	 28.0 %: cytoplasmic
	 20.0 %: nuclear
	  8.0 %: plasma membrane
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for VSF149 is mit
5' end seq. ID VSF149F
5' end seq. 
>VSF149F.Seq
CGGAACGCGTGGGCAAAGAAGCTTCTTTGTAAATAATTTAATTTTCTTATTATATCTTTT
TCTTTTTTTTTTTTAAAAAAAAAACCTTAACCCAAAAAAAAAA----------
Length of 5' end seq. 103
3' end seq. ID VSF149Z
3' end seq. 
>VSF149Z.Seq
----------GTNGGTGGCTACGGTNATGGAGGTGGCCGTNAGGGATGGAGTTCATNATC
AATNTCTTACTTAATACANC
Length of 3' end seq. 70
Connected seq. ID VSF149P
Connected seq. 
>VSF149P.Seq
CGGAACGCGTGGGCAAAGAAGCTTCTTTGTAAATAATTTAATTTTCTTATTATATCTTTT
TCTTTTTTTTTTTTAAAAAAAAAACCTTAACCCAAAAAAAAAA----------GTNGGTG
GCTACGGTNATGGAGGTGGCCGTNAGGGATGGAGTTCATNATCAATNTCTTACTTAATAC
ANC
Length of connected seq. 173
Full length Seq ID -
Full length Seq. -
Length of full length seq. -